• 한국물환경학회지
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• 제26권2호
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• pp.343-348
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• 2010

The objective of this study was to investigate the effects of starvation periods with aerobic or non-aerobic conditions on the organic removal efficiencies and physical characteristics of activated sludge for treating saline and non-saline wastewater. During the experiment, MLSS, MLVSS, sludge volume index (SVI), floc size and fractal dimension, $COD_{Mn}$ removal efficiencies were monitored. The reductions of MLSS, MLVSS and SVI with maintaining the sludge under a non-aerobic condition during starvation periods were smaller than those under a aerobic condition. Floc size, fractal dimension and $COD_{Mn}$ removal efficiencies were less decreased under non-aerobic condition than under aerobic condition. And SVI were strongly correlated with floc size and fractal dimension. Consequently, the result showed that maintaining the activated sludge under non-aerobic starvation conditions was better strategy than that under aerobic starvation conditions as it adapted and resisted to starvation.

• 한국작물학회지
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• 제44권1호
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• pp.32-37
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• 1999

The experiment was conducted to investigate emergence response of lowland weeds at different soil moisture contents, burying depths and upon changes in soil moisture. Rice germination was over 50% at all burying depths under aerobic condition, but the emergence rate of the soil surface placed seeds in saturated and flooded conditions decreased by 19% and 29%, respectively, as compared with that of aerobic condition. Rice seeds at burying depth of over 3 cm did not emerge at all. The emergence rate of Echinochloa crus­galli (L.) Beauv. in aerobic condition was lower than 30%, but the emergence pattern of E. crus galli (L.) Beauv. at different soil moisture contents and seeding depths was similar to that of rice. Emergence behavior of lschaemum rugosum Salisb., Ludwigia octovalvis (Jacq.) Raven and Sphenoclea zeylanica Gaertn. which are dominant lowland weed species in the Philippines also differed depending on soil moisture conditions and burying depths. lschaemum rugosum Salisb. emerged at all burying depths under aerobic condition, whereasLudwigia octovalvis (Jacq.) Raven emerged only at 0 cm deep under saturated and aerobic condition and Sphenoclea zeylanica Gaertn. at 0 cm deep under flooding condition. Weed seeds planted at 1, 3, and 5 cm deep in continuous flooded and saturated condition did not emerge at all, but upon a change of soil moisture condition from saturated to drainage (S$\rightarrow$D) and flooded to drainage (F$\rightarrow$D), grass weeds began to germinate again and the average emergence rate in S$\rightarrow$D and F$\rightarrow$D were 26% and 5% forE. crus­galIi (L.) Beauv., 9% and 8% forI. rugosum SaIisb., respectively. Weed seeds buried in soil in the pot showed great emergence at S$\rightarrow$D but did not emerge under continuous flooded condition. The diversity index accounting for dominance degree and occurrence aspect of weed, was the lowest at F$\rightarrow$D.

• Journal of Microbiology and Biotechnology
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• 제23권4호
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• pp.451-458
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• 2013

The human gastric pathogen Helicobacter pylori (Hp) has been considered a microaerophile. However, we recently reported that, when supplied with 10% $CO_2$, Hp growth is stimulated by an atmospheric level of $O_2$, suggesting that Hp is a capnophilic aerobe. In this study, we investigated the effects of aerobic $O_2$ tension on Hp cells by comparing gene expression profiles of cultures grown under microaerobic and aerobic conditions in the presence of 10% $CO_2$. The results showed that overall differences in gene expression in Hp cells grown under the two $O_2$ conditions were predominantly growth-phase-dependent. At 6 h, numerous genes were down-regulated under the aerobic condition, accounting for our previous observation that Hp growth was retarded under this condition. At 36 h, however, diverse groups of genes involved in energy metabolism, cellular processes, transport, and cell envelope synthesis were highly up- or down-regulated under the aerobic condition, indicating a progression of the cultures from the log phase to the stationary phase. The expression of several oxidative stress-associated genes including tagD, katA, and rocF was induced in response to aerobic $O_2$ level, whereas trxA, trxB, and ahpC remained unchanged. Altogether, these data demonstrate that aerobic $O_2$ tension is not detrimental to Hp cells but stimulates Hp growth, supporting our previous finding that Hp may be an aerobic bacterium that requires a high $CO_2$ level for its growth.

• Journal of Applied Biological Chemistry
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• 제42권2호
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• pp.85-91
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• 1999

The incubation study was conducted under aerobic and anaerobic conditions to study the release of the kinetically labile forms (i. e. chelating ion or anion forms) of Cd, Cu and Zn in sludge-untreated soil ("Control"), sludge 50 and $100dry\;Mg\;ha^{-1}$ treated soils ("Soil-Sludge mixtures"), and sewage sludge ("Sludge"). The chelating ion and anion exchange membranes were embedded into the samples and incubated for 16 weeks under aerobic and anaerobic condition. The total amounts of chelating ion or anionic forms of Cd were too little to be measured during both aerobic and anaerobic incubation. On the other hand, the total amounts of chelating ion or anionic forms of Cu and Zn slightly increased throughout the incubation period under both incubation conditions. For "Control" and "Soil-Sludge mixtures" treatments, the total amounts of Cu and Zn in chelating ion and anion exchange membrane were little difference between aerobic and anaerobic condition, and the total amounts of chelating ion form of Cu and Zn were not different from the those of anionic form of Cu and Zn. However, for "Sludge" treatment, the total amounts of Cu and Zn in anion and chelating ion exchange membrane were greater under aerobic condition than under anaerobic condition, and the total amounts of chelating ion form of Cu and Zn were greater than those of anion form of Cu and Zn under both incubation conditions.

• 한국환경과학회지
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• 제21권12호
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• pp.1455-1462
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• 2012

The vertical distributions of nitrifying bacteria in aerobic fixed biofilm were investigated to evaluate the relationship between nitrification performance and microbial community at different HRT. Fluorescent in situ hybridization (FISH) and portable ion selective microelectrode system were adopted to analyze microbial communities and ions profiles according to the biofilm depth. Cilia media packed MLE (Modified Ludzack-Ettinger) like reactor composed of anoxic, aerobic I/II was operated with synthetic wastewater having COD 200 mg/L and $NH_4{^+}$-N mg/L at HRT of 6 hrs and 4 hrs. Total biofilm thickness of aerobic I, II reactor at 4 hrs condition was over two times than that of 6 hrs condition due to the sufficient substrate supply at 4 hrs condition (6 hrs; aerobic I 380 ${\mu}m$ and II 400 ${\mu}m$, 4 hrs; aerobic I 830 ${\mu}m$ and II 1040 ${\mu}m$). As deepen the biofilm detection point, the ratio of ammonia oxidizing bacteria (AOB) was decreased while the ratio of nitrite oxidizing bacteria (NOB) was maintained similar distribution at both HRT condition. The ratio of AOB was higher at 4 hrs than 6 hrs condition and $NH_4{^+}$-N removal efficiency was also higher at 4 hrs with 89.2% than 65.4% of 6 hrs. However, the ratio of NOB was decreased when HRT was reduced from 6 hrs to 4 hrs and $NO_2{^-}$-N accumulation was observed at 4 hrs condition. Therefore, it is considered that insufficient HRT condition could supply sufficient substrate and enrichment of AOB in all depth of fixed biofilm but cause decrease of NOB and nitrite accumulation.

• Applied Biological Chemistry
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• 제11권
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• pp.167-171
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• 1969

마늘(Allium Sativum L.) 재배(栽培)에 있어서 몇가지 환경조건(環境條件) 즉 통기조건(通氣條件)(완전통기(完全通氣) 및 불완전통기(不完全通氣)) 및 시비량(施肥量)(다비구(多肥區), 관구(慣行區) 및 무비구(無肥區))을 달리했을때 마늘의 생육(生育) 및 인산흡수(燐酸吸收)(표식중과석(標識重過石))에 미치는 영향(影響)을 조사(調査)하였다. 1) 생장(生長)에 있어서 비료(肥料)의 시용수준(施用水準)에 관계(關係)없이 불완전통기구(不完全通氣區)가 초장(草長)이 길었다. 2) 생체중(生體重)은 초장(草長)과 비슷한 경향(傾向)을 보였으나 건물중(乾物重)은 처리간(處理間)에 차이(差異)가 없었다. 3) 인산흡수(燐酸吸收)는 불규칙(不規則)하나마 불완전통기구(不完全通氣區)가 전체적(全體的)으로 증가(增加)하는 경향(傾向)을 보였다. 이상(以上)으로 볼때 마늘의 생육(生育) 및 구(球)의 발육(發育)에 있어서 불완전통기(不完全通氣)가 좋은 결과(結果)를 가져왔다.

• 상하수도학회지
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• 제27권4호
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• pp.457-468
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• 2013

Based on the activated sludge model(ASM), a mathematical model which represents the aerobic sludge digestion by sequencing batch reactor(SBR) combined with ultrasonic treatment was composed and performed in this study. Aerobic digestion using sequencing batch reactor(SBR) equipped with ultrasound treatment was also experimented for the purpose of parameter calibration. Most of the presented kinetic parameters in ASM or ASM2 could be used for the aerobic digestion of sludge but the parameters related in hydrolysis and decay rate needed modification. Hydrolysis rate constant of organic matter in aerobic condition was estimated at $0.3day^{-1}$ and the maximum growth rate for autotrophs in aerobic condition was $0.618day^{-1}$. Solubilization reactions of particulate organics and nitrogen by ultrasonication was added in this kinetic model. The solubilization rate is considered to be proportional to the specific energy which is defined by specific ultrasound power and sonication time. The solubilization rate constant by ultrasonication was estimated at $0.202(W/L)^{-1}day^{-1}$ in this study. Autotrophs as well as heterotrophs also decomposed by ultrasonic treatment and the nitrification reaction was limited by the lack of autotrophs accumulation in the digester.

• 한국환경보건학회지
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• 제30권3호
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• pp.253-258
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• 2004

Synthetic organic polyelectrolytes can be used to condition sludges to enhance their dewaterability. Intermittent aerobic digestion is an useful digestion technology and has many advantages like neutral pH, low installation cost and easiness to operation. The objectives of this study were to investigate the dewaterability of intemittent aerobic digestion sludge and to find the relationship between dewaterability and particle size distribution change under the conditioning of intermittent aerobic digestion sludge by cationic polyelectrolyte. Digested sludge from intermittent aerobic digestion was used and cationic polyacrylamide polymer was added as a conditioner. CST(capillary suction time), TTF(time-to-filtration) were tested as a dewaterability index and the number of particle distribution was analyzed using particle size analyzer. The results indicate that cationic polyelectrolytes is useful to enhance dewaterability of intermittent aerobic digestion sludge. Mean particle diameter was increased as polymer dosage increased and its value was reached up to 100 mm on the condition of optimal cationic polymer dosage. CST and TTF are well correlated with mean particle diameter when the weighting order is 1.7. By the optimal conditioning with cationic polymer, particles in the filtrate are also reduced significantly and this means that conditioning is helpful to main stream by reducing SS loading from return flow.

• 한국환경과학회지
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• 제14권10호
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• pp.955-963
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• 2005

From sludge of S municipal wastewater treatment plant in Busan, Korea, we isolated the denitrifier DN-9 which showed the ability of denitrification under aerobic conditionby the color change and gas formation in liquid culture with Giltay medium. The isolated strain was identified as Pseudomonas sp. DN-9 on the basis of the morphological, physiological, biochemical and nucleotide sequence analysis of l6S rRNA. The isolated strain, Pseudomonas sp. DN-9, has cytochrome $cd_1$, nirS of nitrite reductase. By the co-existance of additional ammonium and nitrate ion, the strain was not affected largely on growth in SL series broth. It seemed the result of denitrification. Although Pseudomonas sp. DN-9 has a good nitrate reduction activity under aerobic condition, the activity is less than Pseudomonas stutzeri in same cultivation condition. However, Escherichia coli had little the activity of aerobic denitrification and Pseudomonas putida showed lower activity of aerobic denitrification than Pseudomonas sp. DN-9 and Pseudomonas stutzeri in this study.

• Bulletin of the Korean Chemical Society
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• 제22권8호
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• pp.903-908
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• 2001

The gene aeg-46.5, which is expressed under anaerobic condition, has putative triple -10 regions and four transcription start sites. The mRNA transcription level and its start point change depending on the aerobic/anaerobic growth condition. RNA polymerase and its regulatory proteins must choose which of three -10 region to use. The putative triple 10 region was mutated to make only one of them function with consensus -10 region sequence (TATAAT) and the other two as non-functional region. The results show that the second and third -10 regions are used for the aerobic/anaerobic expression. The third -10 region is responsible for the high aerobic to anaerobic switch ratio. This suggests that only the last two of the putative triple -10 region have functions on aeg-46.5 gene expression switch control. The phenotype of the mutated promoter was tested in the wild type cell and narL - cell. The results indicate that the control by NarL is independent from the selection of -10 region. The expression patterns on multi-copy plasmids and on single-copy chromosome were compared. These results show that the aerobic/anaerobic switch control of aeg-46.5 is through the choice of -10 region. The mechanism of choosing different -10 region remains to be seen.