• YAKHAK HOEJI
• /
• v.49 no.4
• /
• pp.312-316
• /
• 2005

To develop a new natural antibiotics from Koran plant resources for dealing with the current situation regarding the rapid increase in antibiotic-resistant pathogen, the in vitro inhibitory activities of essential oils from the young leaves of Glehnia littoralis (Umbelliferae) as well as its main constituents were evaluated against susceptible and resistant species of Streptococcus pneumoniae. The essential oil fraction of G. littoralis and its main components, $\alpha-and\;\beta-pinene$, exhibited significant inhibitory activities against the antibiotic-susceptible and resistant strains of S. pneumoniae, with MICs (minimum inhibiting concentrations) ranging from 4.0mg/ml to 16mg/ml. No remarkable differences were shown between the susceptible and resistant strains. Moreover, the disk diffusion test disclosed that these inhibitory activities were dose­dependent. Furthermore, data from the checkerboard titer test with FICIs (fractional inhibiting concentration indices) from 0.15 to 0.28 indicated synergisms between norfloxacin and $\alpha-or\;{\beta}-pinene$ in activity against S. pneumoniae KCCM49629 and S. pneumoniae CCARM4059.

• Advances in Traditional Medicine
• /
• v.10 no.3
• /
• pp.208-213
• /
• 2010

The aerial parts of methanol extract of Oxystelma esculentum (MEOE) (Asclepiadaceae) was evaluated for in vitro lipid peroxidation and antimicrobial activity. Lipid peroxidation was assayed by the change in optical density of the various concentrations (20 - 320 ${\mu}g$/ml) and the percentage inhibition was estimated. Ascorbate/FeSO4-induced peroxidation was inhibited by MEOE and standard antioxidants such as BHA, BHT and the percentage inhibition of the methanol extract was increased with dose dependent manner. The $IC_{50}$ value of the MEOE, BHA and BHT for lipid peroxidation was found to be 135.24 ${\mu}g$/ml, 25.62 ${\mu}g$/ml and 17.13 ${\mu}g$/ml, respectively. The antimicrobial activity of MEOE was determined by disc diffusion method with three grampositive, five gram-negative and two fungal microorganisms. MEOE exhibited the antimicrobial activity against the tested microorganisms except Salmonella typhimurium (MTCC 98). In present study, it is concluded that MEOE has significant effect in the inhibition of lipid peroxidation and possesses good antimicrobial activity.

• Journal of Pharmacopuncture
• /
• v.27 no.3
• /
• pp.223-233
• /
• 2024

Objectives: The phytochemicals in the aerial parts of Euphorbia paralias (also known as Sea Spurge) and their anti-inflammatory and antimicrobial activities were investigated. Methods: The methanolic extract was characterized using GC-MS and HPLC techniques. The anti-inflammatory feature was estimated through a Human Red Blood Cell (HRBC) membrane stabilization technique, while the antimicrobial feature was evaluated by the disc diffusion agar technique, minimum bactericidal concentration, and minimum inhibitory concentration (MIC) via micro-broth dilution method. Results: The GC/MS results demonstrated the existence of various phytochemicals, such as n-hexadecenoic acid, cis-11-eicosenoic acid, and methyl stearate, recognized for their anti-inflammatory and antibacterial features. The similarity of the phytochemical composition with other Euphorbia species emphasizes the genus-wide similarity. The anti-inflammatory activity exhibited a noteworthy inhibitory effect comparable to the reference drug indomethacin. The extract's antimicrobial potential was tested against a range of microorganisms, demonstrating significant action against Gram-positive bacteria and Candida albicans. The quantification of total phenolics and flavonoids further supported the therapeutic potential of the extract. Conclusion: The methanolic extract from E. paralias emerges as a successful natural source of important active constituents with potential applications as anti-inflammatory and antimicrobial agents. This research provides a first step to valorize Euphorbia paralias insights as a source of worthwhile phytochemicals that have potential applications in the pharmaceutical industry.

• Journal of Life Science
• /
• v.16 no.4
• /
• pp.647-652
• /
• 2006

Plants of the genus Dioscorea have long been used as oriental folk medicine, and Dioscorea batatas Decne has been cultivated for healthy food in Korea. Although the bulbils were produced 2,000 ton annually, there are few reports for bioactive compounds in bulbils. In this study, three phenanthrenes and two phenanthraquinones were isolated from the aerial bulbils of D. batatas Decne, and their structures were elucidated. Among them, compound 2 (6-hydroxy-2,7-dimethoxy-1,4-phenanthraquinone) has not been reported previously. Evaluation of antimicrobial activities based on disk-diffusion assay, MIC and MFC showed the compound 12 (6,7-dihydroxy-2,4-dimethoxyphenanthrene) has strong antimicrobial activity with $25\;{\mu}g/ml$ of MIC and MFC against Candida albicans. Our results suggested that compound 12 has a potent antifungal activity, and the antimicrobial activity and its spectrum are modulated by hydroxylation and methoxylation of phenanthrene ring moiety of the compound.

• Korean Journal of Plant Resources
• /
• v.23 no.5
• /
• pp.436-444
• /
• 2010

Methanol extracts of the aerial and rhizome parts obtained from ten species of ferns has been screened for antimicrobial activities against Bacillus subtilis, Escherichia coli, Listeria monocytogenes, Propionibacterium acnes, Streptococcus mutans and Streptococcus sobrinus. Antimicrobial activities were carried out using broth microdilution method and paper disc diffusion assay and the extracts which showed clear zones more than 15mm in concentration of 2 mg/disc were tested for its antimicrobial activities at the $0.125{\sim}2\;mg{\cdot}mL^{-1}$ concentration of each extract for three days. The non-sterilized crude methanol extracts of Osmunda cinnamomea var. fokiensis rhizome showed the highest antimicrobial activities on B. subtilis(39%), E. coli (33%) and L. monocytogenes(58%) at the concentration of $2\;mg{\cdot}mL^{-1}$ after 72 hours. In P. acnes, frond extract of O. cinnamomea var. fokiensis showed most vigorous antimicrobial activities in the all extracts but it showed weak activity(clear zone diameters below 15 mm). All extracts has the antimicrobial activities on Streptococcus, but they exhibited weak activity. At the concentration of $2\;mg{\cdot}mL^{-1}$, only Osmunda japonica rhizome extracts showed 28 and 39% of antimicrobial activities on S. mutans and S. sobrinus after 72 hours and the other extracts showed below 10% of antimicrobial activities on S. mutans and S. sobrinus.

• Korean journal of applied entomology
• /
• v.58 no.4
• /
• pp.271-280
• /
• 2019

We investigated the control efficacy and phytotoxicity of unmanned aerial vehicle-applied pesticides against the insect Spodoptera exigua, a major emerging pest in Chinese cabbage. Phytotoxicity was investigated in cabbage and 7 crops cultivated in the perconducted on 8 surrounding crops including Chinese cabbage at 1 to 2 times the recommended pesticide dosage. We treated cabbage fields with spinetoram suspension concentrate (16×), methoxyfenozide, sulfoxaflor suspension concentrate (16×). Then, we used water-sensitive paper to measure the distribution pattern of falling pesticide particles and the degree of coverage. Two of the pesticides showed 97% control efficacy, however, control efficacy might differ in resistant populations. Phytotoxicity was not observed in Chinese cabbage and the 7 surrounding crops treated with 1 to 2 times the recommended pesticide dosage. Analysis of the distribution pattern of falling pesticide particles revealed that breeze caused particle diffusion. Thus, wind is an important factor affecting the uniform treatment and diffusion of multicopter-applied pesticides. It follows that setting optimal conditions is necessary for effective control and treatment.

• The Journal of the Korea Contents Association
• /
• v.18 no.6
• /
• pp.303-313
• /
• 2018

The purpose of this study is digitalization of earthwork such as development of 3D terrain analysis platform using latest technologies including unmanned aerial vehicle and terrestrial laser scanner to ultimately achieve earthwork automation. It is necessary to develop related element technologies and to establish regulations so that it can be applied to the domestic construction projects. As a result of pilot project about the earthwork surveying automation technology, it was confirmed that information such as terrain coordinates, soil, boring, and excavation volume is acquired smoothly. In this paper, we investigate related regulations and manuals in Japan and propose the improvement plan of domestic regulation. We plan to study regulations from early to final construction stage, combine with the 'regulation for public surveying', and improve the regulations in detail.

• The Journal of the Korea Contents Association
• /
• v.19 no.3
• /
• pp.261-274
• /
• 2019

This paper aims to develop an appropriate methodology for establishing an investment strategy for 'demonstration of artificial rainfall technology using UAV' and that include establishment of a technology classification, set of indicators for technology evaluation, suggestion of final key technology as a whole study area. It is designed to complement the latest research trend analysis results and expert committee opinions using quantitative analysis. The key indicators for technology evaluation consisted of three major items (activity, technology, marketability) and 10 detailed indicators. The AHP questionnaire was conducted to analyze the importance of indicators. As a result, it was analyzed that the attribute of the technology itself is most important, and the order of closeness to the implementation of the core function (centrality), feasibility (feasibility). Among the 16 technology groups, top investment priority groups were analyzed as ground seeding, artificial rainfall verification, spreading and diffusion of seeding material, artificial rainfall numerical modeling, and UAV sensor technology.

• Korean Journal of Remote Sensing
• /
• v.36 no.2_2
• /
• pp.249-261
• /
• 2020

Coastal monitoring using multiple platforms/sensors is a very important tools for accurately understanding the changes in offshore marine environment and disaster with high temporal and spatial resolutions. However, integrated observation studies using multiple platforms and sensors are insufficient, and none of them have been evaluated for efficiency and limitation of convergence. In this study, we aimed to suggest an integrated observation method with multi-remote sensing platform and sensors, and to diagnose the utility and limitation. Integrated in situ surveys were conducted using Rhodamine WT fluorescent dye to simulate various marine disasters. In September 2019, the distribution and movement of RWT dye patches were detected using satellite (Kompsat-2/3/3A, Landsat-8 OLI, Sentinel-3 OLCI and GOCI), unmanned aircraft (Mavic 2 pro and Inspire 2), and manned aircraft platforms after injecting fluorescent dye into the waters of the South Sea-Yeosu Sea. The initial patch size of the RWT dye was 2,600 ㎡ and spread to 62,000 ㎡ about 138 minutes later. The RWT patches gradually moved southwestward from the point where they were first released,similar to the pattern of tidal current flowing southwest as the tides gradually decreased. Unmanned Aerial Vehicles (UAVs) image showed highest resolution in terms of spatial and time resolution, but the coverage area was the narrowest. In the case of satellite images, the coverage area was wide, but there were some limitations compared to other platforms in terms of operability due to the long cycle of revisiting. For Sentinel-3 OLCI and GOCI, the spectral resolution and signal-to-noise ratio (SNR) were the highest, but small fluorescent dye detection was limited in terms of spatial resolution. In the case of hyperspectral sensor mounted on manned aircraft, the spectral resolution was the highest, but this was also somewhat limited in terms of operability. From this simulation approach, multi-platform integrated observation was able to confirm that time,space and spectral resolution could be significantly improved. In the future, if this study results are linked to coastal numerical models, it will be possible to predict the transport and diffusion of contaminants, and it is expected that it can contribute to improving model accuracy by using them as input and verification data of the numerical models.

• Korean Journal of Weed Science
• /
• v.30 no.4
• /
• pp.445-453
• /
• 2010

This study was conducted to investigate herbicidal efficacy and diffusibility of halosulfuron-methyl+mefenacet in water treated with 500 g great granule (GG) and 3 kg granule (GR). The 500 g GG was been spreading on the surface of the water within 6 minutes, 53 seconds, and it's active ingredient was diffused in the water bath of $10m^2$ size between 30 and 60 minutes. In addition, the diffusion of 500 g GG was influenced by moisture contents, so it have to immediately use 500 g GG in paddy fields when it was been unpacked. The herbicidal efficacy of the 500 g GG and 3 kg GR of halosulfuron-methyl+mefenacet was excellent to most weed species, but showed different efficacy for the control of Aneilema keisak and Scirpus juncoides, that was may be distribution pattern of active ingredient as different formulation.