• Journal of the Korean Applied Science and Technology
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• v.41 no.3
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• pp.543-554
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• 2024

In this study, the anti-inflammatory, anti-bacterial and anti-oxidative activities of subcritical water extract from Chamaecyparis obtusa leaves was compared with hot water extract. The yield of subcritical water extract (165℃ and 180℃, 80 bar) was 39.4~48.5%, higher than 31.5% of hot water extract. In the anti-inflammatory tests using lipopolysaccaride (LPS)-induced RAW264.7 macrophages, the subcritical water extract concentration-dependently inhibited production of nitric oxide (NO) without causing cell toxicity. Upon the anti-bacterial studies using Cutibacterium acnes and Staphylococcus epidermidis, subcritical water extract showed the stronger activity than hot water extract. In addition, DPPH and ABTS cation radical scavenging activity experiments showed that the radical scavenging activity of subcritical water extract was similar to that of hot water extract. Moreover, in the study of cell protection effect using HaCaT keratinocytes damaged by hydrogen peroxide (H₂O₂), the subcritical water extract (165℃, 80 bar) indicated protective effect against oxidative stress. These results suggested that the subcritical water extract of C. obtusa leaves as natural ingredients could be used as anti-inflammatory, anti-bacterial and anti-oxidative ingredients in cosmetic formulations.

• Journal of the Korean Recycled Construction Resources Institute
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• v.12 no.3
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• pp.281-289
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• 2024

In this study, CO₂-immobilized desulfurized gypsum(CFDG) was applied to ground granulated blast furnace slag to examine the basic properties of mortar and concrete, and to evaluate its responsiveness through field demonstration test. CFDG had a relatively circular composition compared to desulfurized gypsum(DG), and its main components were CaO 47.6 % and SO₃ 22.1 %. As a result of mortar and concrete tests, the flow tended to increase and the compressive strength was at the same level. In addition, the target properties of concrete for application to farm roads, which were a slump of 120±25 mm and a compressive strength of 24 MPa, were satisfied with a slump of 135 mm and a compressive strength of 42.1 MPa at 28 days. In February 2024, an on-site demonstration of a farm road was conducted in Seongmun-myeon, Dangjin-si, and as a result of reviewing the compressive strength according to curing conditions, the physical properties and durability of unhardened concrete, the target results were satisfied, expanding the use of CFDG by applying fine powder of blast furnace slag and carbon reduction can be expected.

• Journal of the Korean Recycled Construction Resources Institute
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• v.12 no.3
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• pp.254-262
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• 2024

The demand for high strength and ultra-lightweight materials to incorporate the advanced technology of nanomaterials into the lengthening of structures is continuously increasing. Therefore, based on existing research results and numerous mixing trials, we derived a mix of high strength and ultra-light concrete of a compressive strength of 100 MPa with a unit weight of 18 kN/m³ and a compr essive str ength of 80 MPa with a unit weight of 16 kN/m³ and evaluated their per for mance. In this paper, 108 specimens corresponding to high strength and ultra-lightweight concrete with a compressive strength of 100 MPa under a unit weight of 18 kN/m³, and a compressive strength of 80 MPa under a unit weight of 16 kN/m³ were manufactured, and the bond characteristics were identified by performing a directly tensile tests, and the bond characteristics were evaluated by comparing them with the experimental results and the current design criteria. It was judged that the bond strength calculation formula of ACI-408R and the experimental results were not accurately reflected, so an bond stress equation based on ACI-408R was proposed. The result of the proposed equation was that the deviation was somewhat reduced. In addition, the results of calculating the CEB-FIP model and the modified CMR model using statistical analysis showed slight differences from the experimental results, but considering that the bond behavior is a local behavior, the proposed model appears to explain the bond behavior of high strength and ultra-light concrete as a whole.

• The Journal of Korean Obstetrics and Gynecology
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• v.26 no.1
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• pp.1-24
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• 2013

Objectives: The object of this study was to observe the protective effect of Gamijipaesan aqueous extracts(GJS), which has been traditionally used in Korean medicine in obstetrics & gynecological fields as anti-infectious and anti-inflammatory agents, against mastitis induced by Staphylococcus aureus infection in a rat model through antibacterial, antiinflammatory, immunomodulatory, and anti-oxidant effects. Methods: Antibacterial activities of GJS against S. aureus were detected using standard agar microdilution methods, with the effects on the bacterial invasion and intracellular killing of individual test materials in human mammary gland carcinoma cell(MCF-7) and murine macrophages(Raw 264.7) at MIC1/2, MIC and MIC2 concentration levels. In addition, the effects on the cell viability, nitric oxide(NO), tumor necrosis factor(TNF)-${\alpha}$ and interleukin (IL)-6 productions of LPS activated Raw 264.7 cells. The changes on the mammary tissue viable bacterial numbers, myeloperoxidae(MPO), inducible nitric oxide synthetase(iNOS), TNF-${\alpha}$ and IL-6 contents were observed in the S. aureus in vivo intramammary infectious rat model. The anti-bacterial and anti-inflammatory effects were compared with ciprofloxacin and piroxicam, respectively in the present study. Results: MIC of GJS and ciprofloxacin against S. aureus were detected as $0.860{\pm}0.428$ (0.391-1.563) mg/ml and $0.371{\pm}0.262$(0.098-0.782) ${\mu}g/ml$, respectively. In addition, GJS and ciprofloxacin were also showed marked dosage-dependent inhibition of the both bacterial invasion and intracellular killing assays using MCF-7 and Raw 264.7 cells at MIC1/2, MIC and $MIC{\times}2$ concentrations, respectively. $ED_{50}$ against LPS-induced cell viabilities and NO, TNF-${\alpha}$ and IL-6 releases of GJS were detected as 0.72, 0.04, 0.08 and 0.11 mg/ml, and as 19.04, 4.18, 5.37 and 4.27 ${\mu}g/ml$ in piroxicam, respectively. 250 and 500 mg/kg of GJS also inhibit the intramammary bacterial growth, MPO, iNOS, TNF-${\alpha}$ and IL-6 contents in S. aureus in vivo intramammary infected rats, respectively. GJS 500 mg/kg showed quite similar antibacterial and anti-infectious effects as compared with ciprofloxacin 40 mg/kg and also showed similar anti-inflammatory effects as piroxicam 10 mg/kg, in S. aureus in vivo intramammary infectious models. Conclusions: The results obtained in this study suggest that over 250 mg/kg of GJS showed favorable anti-infectious effects against S. aureus infection in a rat model through their antibacterial, anti-inflammatory, immunomodulatory and anti-oxidant effects and therefore expected that GJS can be used as alternative therapies, having both anti-inflammatory and anti-infectious activities. However, more detail mechanism studies should be conducted in future with the efficacy tests of individual herbal composition of GJS and the screening of the biological active compounds in individual herbs. In the present study, GJS 500 mg/kg showed quite similar anti-infectious effects were detected as compared with ciprofloxacin 40 mg/kg treated rats, and also GJS shows quite similar anti-inflammatory effects as compared with piroxicam 10 mg/kg in S. aureus in vivo intramammary infectious rats, but ciprofloxacin did not showed any anti-inflammatory effects, and piroxicam did not showed anti-infectious effects in this study.

• Korean Journal of Soil Science and Fertilizer
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• v.1 no.1
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• pp.117-123
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• 1968

A simplest and best method to find out lime requirement for Korean soils is described. Twenty six top soil samples were collected from various localities and chemical characteristics were dertermined. Three different buffer curve methods were employed as the standard to find out the most reliable test for lime requirement. In addition, the lime requirement test by the Shoemaker's and Woodruff's as well as the O.R.D. kit method were compared with the most reliable buffer curve method. The results may be summarized as follows. 1. The regression equation of the most reliable lime requirement by buffer curve method, y, on percent base saturation of various soils, X, was y=9.69-0.106 X and the negative correlation coefficient r=-0.84 was statistically significant at 5% level. 2. Lime requirement test by the best, buffer curve, method was more well correlated with the sum of exchangeable hydrogen and exchangeable aluminum contents of the soil samples than the exchangeable hydrogen content only. 3. The ordinary buffer curve method for lime requirement is more reasonable than the lime requirement test by the buffer curve prepared after addition of 1 normal potassium chloride or calcium chloride solutions. 4. The lime requirement tests by the most reasonable buffer curve method, X, and by the Woodruff's, the Shoemaker's and O.R.D. kit were compared. The best method for laboratory lime requirement test was the Shoemaker's method and the O.R.D. kit method also was good enough for the field test and these two method are recommended as the unified method of lime requirement test in each soil testing laboratories throughout Korea.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.12
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• pp.2263-2273
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• 2000

Catalytic wet air oxidation of acetic acid over Mn-Ce based catalysts deposited on various supports ($SiO_2$, $TiO_2$, $ZrO_2$), $ZrSiO_4$, $ZrO_2(10wt%)/TiO_2$) have been carried out in high pressure microreactors. Also, promotional effects by small addition(O.5~1.0 wt%) of p-type semiconductors (CoO, $Ag_2O$, SnO) have been investigated. From the screening tests for initial activity ranking, both Mn(2.8)-Ce(7.2 wt%) and Ru(O.4)Mn(2.7)-Ce(6.9 wt%) supported on $TiO_2$ were selected as the promising reference candidates. In $Mn-Ce/TiO_2$ reference catalyst, addition of small amount of each p-type semiconductor (Co, Sn and Ag) resulted in activity promotional effect and the degree of the increase was in the following order: Co> Ag > Sn. Especially, $Mn-Ce/TiO_2$ promoted with 0.5 wt% Co gave the 2.6 folds activity increase compared to the reference case attributing to the surface area increase as well as synergy effect. In $Ru-Mn-Ce/TiO_2$ reference catalyst, only Co(1.0 wt%) promoted case showed a little reaction rate increase.

• The Korean Journal of Mycology
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• v.46 no.3
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• pp.295-306
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• 2018

In the present study, we compared the effects of 50% ethanolic extracts of Chinese and Korean Wolfiporia cocos (CPE and KPE) on in vitro lipid accumulation in 3T3-L1 cells and their anticancer activities in Sarcoma 180 cells. We further compared the anticancer activities and the 50% inhibitory concentrations ($IC_{50}$) of CPE with KPE with cultivated for one and two years in a landfill and a facility (LPE and FPE), respectively. In addition, the single oral dose toxicities of CPE and KPE were evaluated in mice. Lipid accumulation was inhibited after 48 hours, in CPE and KPE treated 3T3-L1 cells; however, no significant difference was observed between CPE and KPE in their lipid accumulation inhibitory activities. The anticancer activity of KPE was higher than that of CPE at $300{\mu}g/mL$ (p<0.05), revealing the possibility of an auxiliary biological means for origin identification. The anticancer activities of LPE and FPE were significantly stronger than that of CPE (p<0.05) but there was no difference between extracts from one- and two-year-old W. cocos, irrespective of the cultivation method. In single oral dose toxicity tests, CPE and KPE did not induce mortality during the 14-day observation. Thus, the 50% of lethal dose ($LD_{50}$) of CPE and KPE were estimated to be higher than 2,000 mg/kg. Taken together, our results indicate that the anticancer assay could be an auxiliary means of identifying the origin of W. cocos. In addition, artificial cultivation could be an alternative way to reduce the import of W. cocos. Lastly, 50% ethanolic W. cocos extracts could be potential candidates for obesity and cancer managements.

• The Korean Journal of Nuclear Medicine
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• v.34 no.3
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• pp.183-198
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• 2000

Purpose: The purpose of this study was to evaluate alterations of regional cerebral blood flow (CBF) and cerebrovascular reserve (CVR), and correlation between these alternations and cognitive dysfunction in patients with chronic traumatic brain injury (TBI) and normal brain MRI findings. Materials and Methods: Thirty TBI patients and 19 healthy volunteers underwent rest/acetazolamide brain SPECT using Tc-99m HMPAO. Korean-Wechsler Adult Intelligence scale test was also performed in the patient group. Statistical analysis was performed with statistical parametric mapping software (SPM'97) Results: CBF was diminished in the left hemisphere including Wernicke's area in all patients with lower verbal scale scores. In addition, a reduction in CBF in the right frontal, temporal and parietal cortices was related with depressed scores in information, digital span, arithmetic and similarities. In patients with lower performance scale scores, CBF was mainly diminished in the right hemisphere including superior temporal and supramarginal gyri, premotor, primary somatomotor and a part of prefrontal cortices, left frontal lobe and supramarginal gyrus. CVR was diminished in sixty-four Brodmann's areas compared to control. A reduction in CVR was demonstrated bilaterally in the frontal and temporal lobes in patients with lower scores in both verbal and performance tests, and in addition, both inferior parietal and occipital lobes in information subset. Conclusion: Alterations of CBF and CVR were demonstrated in the symptomatic TBI patients with normal MRI finding. These alterations were correlated with the change of intelligence, of which the complex functions are subserved by multiple interconnected cortical structures.

• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.133-145
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• 1984

To study the effect of foaming agent on the characteristic of mortar, the tests of water-cement ratio and bulk density of mortar were done under the different mixing ratio with G. U and J foaming agents. The results obtained were summarized as follows: 1. At the mixing ratio of 1 : 4 and 0.5% of foaming agent, the highest water-cement ratio was 90% by G, 88.3% by U and 70% by J foaming agent, respectively, being lower than 91.6% of that of cement mortar. 2. At the mixing ratio of 1 : 3 and 3.0% of foaming agent, the water-cement ratio was decreased up to 22.0% by G and 24.1% by U foaming agent, respectively, but it gradually was increased in richer and poorer mixing ratio. At the mixing ratio of 1 : 4 and 3.0% of foaming agent, the water-cement ratio was decreased up to 53.1% by J foaming agent, but it gradually was increased in richer mixing ratio. 3. At the mixing ratio of 1 : 1 and 0.5% of foaming agent, the highest bulk density was $1.981g/cm^3$ by G, $1.863g/cm^3$ by U and $1.149g/cm^3$ by J foaming agent, respectively, being lower than $2.048g/cm^3$ of that of cement mortar. 4. At the mixing ratio of 1 : 2 and 3.0% of foaming agent, the bulk density was decreased up to 20.7% by G, 23.7% by U and 56.5% by J foaming agent, respectively, but it gradually was increased in richer and poorer mixing ratio. 5. The water-cement ratio and bulk density were decreased in more addition of foaming agent, respectively, multiple regression equations of water-cement ratio and bulk density were computed depending on a function of mixing ratios and addition of foaming agents.

• Korean Journal of Agricultural Science
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• v.10 no.2
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• pp.310-323
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• 1983

To study the effect of foaming agent on the production and property of light weight concrete, the tests of compressive, tensile, bending strengths and absorption rates of mortar were done under the different mixing ratio with J, A and D foaming agents. The results obtained were summarized as follows : 1. The strengths were decreased in richer mixing ratio and more addition of foaming agent. The decrease of strengths was the greatest at the level of 0.75% of foaming agent. The decreasing rate of strengths was in order of J, A and D foaming agent. 2. At the mixing ratio of 1:1, ${\sigma}_{28}$ and 0.75% of foaming agent, the compressive strength was decreased up to 34.9% by D, 47.8% by A and 86.8% by J foaming agent, respectively, the tensile strength was decreased up to 14.8% by D, 20.2% by A and 77.9% by J foaming agent, respectively, bending strength was decreased up to 19.9% by D, 35.0% by A and 79.1% by J foaming agent, respectively. The decrease of compressive strength was more severe than that of tensile and bending strengths. 3. The absorption rates were increased in poorer mixing ratio and more addition of foaming agent. The absorption rate was significantly higher at the early stage of immersed water. The absorption rate was in order of J, A and D foaming agent. 4. The decrease of strengths was inevitable in cement-mortar with foaming agent, but the cement mortar with foaming agent has such the properties of the light-weight, lnsulation, Keeping-warmth, sound proof and fire-proof that if could be utilized to the constructions which need low strengths.