• Biomolecules & Therapeutics
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• 제11권2호
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• pp.126-132
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• 2003

Efficacy and in vivo bioassay of recombinant human erythropoietin (rh-EPO, DWP413) was investigated. Efficacy studies on erythropoiesis were conducted in normal, cisplatin-induced anemic rats and acute hemorrhage - induced anemic rats. Animals were treated intravenously with DWP413 for 5 days, the changes in the number of red blood cells (RBC), hematocrit value (Hct), hemoglobin concentration (Hb) and reticulocyte were examined. In normal rats, at the doses of 50, 250, and 1250 IU/kg/day, in cisplatin-induced anemic rats, at the doses of 50, 100 and 200 IU/kg/day, RBC, Hb, Hct and reticulocyte were increased dose-depen-dently. And in acute hemorrhage-induced anemic rats, DWP413 (150, 450 and 1350 IU/kg/day) significantly increased RBC, Hb, Hct and reticulocytes. In histopathological findings of kidney, cisplatin alone treated rats expressed severe glomerulus and tubular damage. But in the DWP413 treated rats after cisplatin treatment, these were not remarkable compared to cisplatin alone treated rats. In vivo bioassay, DWP413 had 102.43% of bioactivity compared to erythropoietin BRP(Biological Reference Product, European Directorate for the Quality of Medicines). These results suggest that DWP413 might be useful for the therapy of anemia induce by renal failure and acute blood loss.

• 생명과학회지
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• 제19권7호
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• pp.878-885
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• 2009

급성 폐손상과 급성 호흡곤란 증후군은 치사율이 매우 높은 질환임에도 불구하고 현재까지 뚜렷한 치료법이 확립되지 않아서 조기진단에 많은 관심을 기울이고 있다. 본 실험에서는 출혈성 쇼크로 유발되는 급성 폐손상 모델에서 철대사를 조절하는 인자로 알려진 heme oxygenase-1 (HO-1)과 ferritin의 변화 양상을 알아보고 급성 폐손상 또는 급성 호흡곤란 증후군의 조기진단인자로서 적합한지를 알아보고자 하였다. 실험동물은 체중 300-450g의 sprague-Dawley rat을 사용하였으며, 급성폐손상과 ferritin 및 HO-1변화의 관계를 알아보기 위하여 정상군(Sham), 출혈군 및 phospholipase A$_2$ 억제제인 mepacrine (60mg/kg, iv)을 전처치한 출혈군으로 나누어 실험하였다. Sham군은 출혈군과 동일하게 수술하고 출혈은 시키지 않았으며 나머지 과정은 출혈군과 동일하게 처리하였다. 출혈은 withdrawal pump를 이용하여 분당 4ml/kg의 속도로 5분간 총 체중kg 당 20ml의 혈액을 대퇴동맥에 연결한 관을 통하여 출혈시켰다. 출혈로 인하여 급성 폐손상이 유발되었으며 이는 mepacrine 전처치로 유의하게 억제되었다. 출혈 후 혈장 단백질 농도는 감소하였으나 혈장 ferritin 농도는 출혈 60분 후부터, HO-1 농도는 90분 후부터 증가하여 2시간 후에는 Shanm군에 비해 크게 증가하였으며, 이는 mepacrine 전처치한 경우에서 유의하게 둔화되었다. 폐세척액 내의 세포에서 ferritin과 HO-1의 발현량은 출혈군에서 가장 크게 나타났고, mepacrine을 전 처치한 출혈군에서 발현량이 줄어들었다. 이상의 결과로 살펴볼 때 혈장 ferritin및 HO-1은 출혈성 쇼크로 유발되는 급성 폐손상의 정도와 밀접한 상관관계를 가지고, 비록 정도의 차이는 있더라도 폐세척액 내의 염증성 세포에서도 발현량이 증가하는 것을 관찰할 수 있었다. 그러므로, 혈장의 ferritin 및 HO-1농도를 급성 폐손상 및 급성 호흡곤란 증후군의 조기진단을 위한 간접적인 생체지표로 사용할 수 있을 것으로 보이며, 이 실험 모델에서는 ferritin이 HO-1보다 더 예민한 인자로 평가된다.

• Restorative Dentistry and Endodontics
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• 제48권3호
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• pp.29.1-29.8
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• 2023

Objectives: This study tested the hypothesis that cryotherapy duration influences lipopolysaccharide (LPS)-induced inflammation in a rat model. Materials and Methods: Six Wistar rats (Rattus norvegicus albinus) were used. Five sites were selected per animal and divided into 5 groups: a negative control group (NC), 2 positive control groups (PC1 and PC2), and 2 experimental groups (E1 and E2). Cryotherapy was applied for 1 minute (E1) or 5 minutes (E2). An acute inflammatory response was induced in the PC and E groups via subcutaneous administration of 0.5 mL/kg. In the PC2 group, a catheter was inserted without additional treatment. For the E1 and E2 groups, 2.5℃ saline solution was administered through the implanted catheters for 1 and 5 minutes, respectively. The rats were sacrificed, and samples were obtained and processed for histological analysis, specifically examining the presence of polymorphonuclear neutrophils and hemorrhage. The χ² test was used to compare the presence of acute inflammation across groups. Dependent variables were compared using the linear-by-linear association test. Results: Inflammation and hemorrhage varied significantly among the groups (p = 0.001). A significantly higher degree of acute inflammation was detected (p = 0.0002) in the PC and E1 samples than in the E2 group, in which cryotherapy was administered for 5 minutes. The PC and E1 groups also exhibited significantly greater numbers of neutrophils (p = 0.007), which were essentially absent in both the NC and E2 groups. Conclusions: Cryotherapy administration for 5 minutes reduced the acute inflammation associated with LPS and catheter implantation.

• 대한한의학회지
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• 제32권6호
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• pp.74-84
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• 2011

Objectives: Palmul-tang (hachimotsu-to in Japanese and bawu-tang in Chinese) is a mixture of eight herbs. It is traditionally used for the treatment of anemia, anorexia, general weakness, and female infertility in China, Japan, and Korea. In this study, we investigated the protective effects of Palmul-tang water extract (PTE) against ethanol-induced acute gastric injury in rats. Material and Methods: Acute gastric lesions were induced by intragastric administration of 5mL/kg body weight of absolute ethanol to each rat. Control group rats were given PBS orally and the ethanol group (EtOH group) received absolute ethanol (5mL/kg) by oral gavage. The positive control group and the PTE group were given oral doses of omeprazole (50mg/kg) or PTE (400mg/kg), respectively, 2 h prior to the administration of absolute ethanol. The stomach of each animal was excised and examined for gastric mucosal lesions. To confirm the protective effects of PTE, we evaluated the degree of lipid peroxidation, the level of reduced glutathione (GSH), and the activities of the antioxidant enzymes catalase, glutathione-S-transferase, glutathione peroxidase, and glutathione reductase in the stomach. Results: PTE reduced ethanol-induced hemorrhage and hyperemia in the gastric mucosa. PTE reduced the increase in lipid peroxidation associated with ethanol-induced acute gastric lesions and increased mucosal GSH content and the activities of antioxidant enzymes. Conclusion: These results indicate that PTE protects gastric mucosa against ethanol-induced acute gastric injury by increasing antioxidant status. We suggest that PTE could be developed as an effective drug for the treatment of acute gastric injury.

• 약학회지
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• 제40권5호
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• pp.591-598
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• 1996

The water extracts of ten crude drugs were tested for the spasmolytic and anti-peptic ulcer activities on rat ileum smooth muscle contraction and aspirin-induced acute hemorrhag ic erosive gastritis respectively. The water extract of Aurantii immaturi pericarpium(AIP)($IC_{50}=1.5{\times}1O^{-2}$g/l), Aurantii nobilis pericarpium(ANP)($IC_{50}=2.5{\times}1O^{-2}$g/l), Cyperi rhizoma(CR)($IC_{50}=3.3{\times}1O^{-2}$g/l). Linderae radix(LR) ($IC_{50}=6.8{\times}1O^{-2}$), Aurantii fructus immaturus(AFI)($IC_{50}=11.8{\times}1O^{-2}$), Saussureae radix(SR)($IC_{50}=13.2{\times}1O^{-2}$g/l) and Ponciri fructus(PF)($IC_{50}=23.3{\times}1O^{-2}$g/l) showed inhibitory activity on the isometric contraction of rat ileum smooth muscle induced by electrical stimulation in a concentration-dependent manner, whereas the water extracts of Arecae pericarpium(AP), Agastachis herba(AH) and Magnoliae cortex(MC) potentiated the isometric contraction. In the aspirin-induced acute gastritis, the water extracts of MC, AP and CR reduced significantly the gastric juice secretion, gastric juice acidity and pepsin activity. They also showed protective activity of gastric mucosal layer from erosion and petichial hemorrhage in gross and histological examination.