• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2004년도 정기총회 및 제33차 춘계 학술대회
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• pp.353-356
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• 2004

Rotavirus and Norovirus are major causative agents of acute diarrhea and gastroenteritis. In our study, Each viral RNA was isolated from the feces of patients for viral diarrhea in Korea, respectively. And cDNA library were constructed using RT-PCR. Also, cDNAs encoding VP8 derived from Rotavirus and Capsid protein derived from norovirus were subesequently cloned and expressed in Echerichia coli as a fusion antigen. Molecular weight of fusion antigen was approximately 60kDa. Also, substantial overexpression was accomplished. We yielded egg yolk lgY which is potentially useful in controlling of Rotavirus and Norovirus which are one of the most prevalent pathogenic viruses.

• 한국동물위생학회지
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• 제29권4호
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• pp.497-501
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• 2006

Acute hemorrhagic enteritis was diagnosed in a seven-month-old male Shitzu dog dying of blood stained diarrhea and vomiting. Clinical findings were anorexia, dullness and sudden death after massive bloody diarrhea. At necropsy, main lesion was the hemorrhage in small intestine, mainly duodenum and jejunum. Microscopically, Gram positive long bacilli were massively detected on the mucose epithelial cells and necrotic debris of small intestine. Coagulative necrosis of epithelial cells and thrombosis of small intestine were also identified. However, there was no lesion of crypt epithelium. Mineral infiltration in both gastric mucosa and renal tubules was detected and proliferation of fibrous tissue was also shown in corticomedullary regions. In bacterial examination, C perfringens was isolated in anaerobic culture and it was confirmed to type A by multiplex PCR. Therefore, the dog was diagnosed as C perfringens type A associated enteritis with uremia.

• 한국임상수의학회지
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• 제19권3호
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• pp.379-382
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• 2002

A patient (Pointer+Viszula mixed breed, 4 months old, male), with chief historic sign of acute vomiting, hemorrhagic diarrhea, anorexia and dyspnea during 4 days was admitted to College of Veterinary Medicine, Kyungpook National University. Necropsy findings were revealed ulcer and hemorrhage contained hemorrhagic diarrhea in gastrointestinal tract, severe emphysema and hemorrhage in the lung and kidney and cardiac hypertropy. Histopathological changes showed emphysema, hyperemia and hemorrhage in the lung, severe hyperemia, hemorrhage, hepatic vacuolation and cellular necrosis in the liver, hyperemia, hemorrhage, necrosis of tubular epithelium in the kidney, hemorrhage in cardiac muscle and hyperemia, necrosis and sloughing of epithelium in the intestine. Histopathological diagnosis was made as paraquat poisoning.

• Childhood Kidney Diseases
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• 제26권1호
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• pp.58-62
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• 2022

Atypical hemolytic uremic syndrome (aHUS) is a rare disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, and acute kidney injury without any association with preceding diarrhea. Dysregulation of the complement system is the most common cause of aHUS, and monoclonal humanized anti-C5 antibodies are now recommended as the first-line treatment for aHUS. However, if the complement pathway is not the cause of aHUS, C5 inhibitors are ineffective. In this study, we report the second reported case of aHUS caused by DGKE mutations in Republic of Korea. The patient was an 11-month-old infant who presented with prodromal diarrhea similar to typical HUS, self-remitted with conservative management unlike complement-mediated aHUS but recurred with fever. While infantile aHUS often implies genetic dysregulation of the complement system, other rare genetic causes, such as DGKE mutation, need to be considered before deciding long-term treatment with C5 inhibitors.

• Clinical and Experimental Pediatrics
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• 제46권12호
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• pp.1217-1223
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• 2003

목 적 : 전라북도 소아과 개원의의 급성 위장염에 대한 이해와 환자의 진단과 치료에 대한 경험과 인식을 알아보고 최근 개발된 로타 바이러스 백신에 대한 인식을 알아보고자 하였다. 방 법 : 2002년 4월부터 6월까지 전라북도에서 소아과 의원을 개원하고 있는 소아과 전문의를 대상으로 미리 작성된 설문지를 이용하여 우편배달을 통해 조사하였다. 배포된 설문지 수는 총 82매였고 이 중 63명의 소아과 전문의가 응답하여 회수율은 76.8%였다. 급성 위장염 환자의 비율, 선호하는 치료제 및 방법, 항생제 사용빈도 및 종류, 항생제 사용을 고려하는 임상소견, 급성 위장염 환자에서 시행해 보고 싶은 검사, 로타 바이러스 백신에 대한 관심도와 사용 계획에 대해 조사하였다. 결 과 : 급성 위장염 환자는 연중 발생하였으며 겨울과 봄에 약간 많았다. 주로 사용하는 치료제로는 유산균 제제(84.1%), 경구용 수액제(79.4%), 효소제(58.8%)를 선호하였다. 대부분 의사들이 전체 위장염 환자의 20% 이하에서 항생제를 사용하였고, 주로 사용하는 항생제는 amoxicillin(61.3%)과 trimethoprim/sulfamethoxazole(39.7%)이였으며, 항생제 사용을 고려하는 임상증상은 혈변이나 점액변, 고열 등의 증상이었다. 급성위장염 환아에서 중요하다고 생각하거나 가능하면 해보고 싶은 검사는 대변 검사(로타 바이러스 항원, 적혈구, 백혈구), 복부 방사선 검사, 혈청 전해질 검사 등이었다. 대부분의 의사들이 로타 바이러스 백신에 대해 긍정적인 관심을 가지고 백신이 도입될 경우 사용하겠다고 하였으며, 백신사용을 결정할 때 중요하게 생각하는 점으로는 예방효과와 부작용이었다. 결 론 : 전라북도 소아과 개원의들의 급성 위장염 환자에 대한 인식은 대체로 긍정적인 것으로 판단되었으며, 앞으로 보다 적극적인 검사방법의 개발과 이용이 중요할 것으로 생각된다.

• Toxicological Research
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• 제21권1호
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• pp.63-70
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• 2005

Gami-Honghwa-Tang (KH-19), a herbal prescription for reducing the side effect of radiotherapy, is composed of eight crude herbs such as Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Cnidii Rhizoma, Paeoniae Radix, Corni Fructus, Moutan Cortex Radicis, Lycii Fructus, Carthami Flos, and Glycyrrhizae Radix. In this study, marker substances in KH-19 were analyzed by high performance liquid chromatography-diode array detector (HPLC-DAD) and safety evaluation of standardized KH-19 was evaluated by good laboratory practices (GLP) guideline of Korea Food and Drug Administration. HPLC-DAD was employed to determine the quantities and the qualities of several marker substances such as 5-hydroxymethyl-2-furaldehyde (5-HMF), paeonol, loganin, paeoniflorin, glycyrrhizin, and decursin in the KH-19. In acute oral toxicity study on rat, transient inhibition of body weight was shown, but change in general symptom was not detected. No dead animal was observed up to 5,000 mg/kg in both male and female animals. In acute oral toxicity study on Beagle dog, transient vomiting, diarrhea, anorexia, and body weight reduction were observed, However, no dead animal was observed up to 2,000 mg/kg in both male and female animals.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제17권4호
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• pp.263-265
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• 2014

Epiploic appendagitis is an inflammation of the epiploic appendage in which the small sacs projecting from the serosal layer of the colon are positioned longitudinally from the caecum to the rectosigmoid area. Epiploic appendagitis is rare and self-limiting; however, it can cause sudden abdominal pain in children. Epiploic appendagitis does not typically accompany other gastrointestinal diseases. Here, we report on a healthy eight-year-old girl who presented with abdominal pain, fever, vomiting, and diarrhea. Based on these symptoms, she was diagnosed with acute gastroenteritis, but epiploic appendagitis in the ascending colon was revealed in contrast computed tomography (CT). The patient was treated successfully with conservative management. CT is beneficial in diagnosis and further assessment of epiploic appendagitis. Pediatricians need to be aware of this self-limiting disease and consider it as a possible alternate diagnosis in cases of acute abdominal pain.

• 한국임상수의학회지
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• 제23권3호
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• pp.267-271
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• 2006

The present study was performed to clarity the effect of hemodialysis(HD) on acute renal failure induced by bilateral ligation of the ureter. HB was applied on 48 hours(1st HD) and 72 hours(2nd HD) after ligation of the ureter, respectively. Clinical signs including nausea, vomiting, diarrhea and depression were observed in all cases(6 heads), however, those symptoms were much improved after HD. Hematological values including WBC, PCV and PLT were increased after ligation of the ureter, however, those values were decreased by HD without significance, compared with those of before HD. In addition, serum BUN, creatinine, Ca and P levels were increased after ligation of the ureter, however, BUN(p<0.05), creatinine(p<0.05) and P(p<0.05) were significantly decreased by HD, compared with before HD, respectively. Considering above findings, it was thought that HD was effective for improvement of clinical symptoms of dogs with induced acute renal failure.

• Childhood Kidney Diseases
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• 제10권2호
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• pp.244-248
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• 2006

Shigella infection usually produces gastrointestinal symptoms but rarely causes urinary tract infection. A 7-year-old girl was admitted for fever, chills, right flank pain, and dysuria. She had no vomiting or diarrhea. There was mild tenderness in her right lower abdomen, and right CVA tenderness was also noted. Acute pyelonephritis was diagnosed by abdominal CT. She showed improvement with intravenous administration of antibiotics. The first urine culture grew $1{\times}10^5$ CFU/mL Shigella dysenteri. Although urinary tract infections due to Shigella species are extremely rare, Shigella species should be considered as a possible cause of pediatric urinary tract infection. We report the first case of urinary tract infection caused by S. dysenteri, which presented as acute pyelonephritis without gastrointestinal symptoms in a child.

• 한국수산과학회지
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• 제47권4호
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• pp.356-362
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• 2014

Phlorotannins, the major constituent in brown algae, possess various biological activities; however, there is little information their toxicological effects. To assess the safety of phlorotannins, we investigated the acute oral toxicity of a high-purity phlorotannin preparation (PRT; total phlorotannin content 90%) in beagle dogs. Six beagle dogs (3 males, 3 females) were assigned randomly to three experimental groups. PRT was administered at oral doses of 250, 500, and 750 mg/kg by capsule. Vomiting by male and female beagles was observed with 500 and 750 mg/kg on the first day. In addition, one beagle given 750 mg/kg had soft stool and diarrhea on days 3 and 13. No deaths or abnormal clinical signs were observed during the experiment. All groups showed similar body weight gain and food consumption. Our acute toxicity study showed that PRT did not cause any toxicological effects in beagle dogs.