• Molecules and Cells
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• 제42권11호
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• pp.810-819
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• 2019

For physiological or pathological understanding of bone disease caused by abnormal behavior of osteoclasts (OCs), functional studies of molecules that regulate the generation and action of OCs are required. In a microarray approach, we found the suppression of tumorigenicity 5 (ST5) gene is upregulated by receptor activator of nuclear $factor-{\kappa}B$ ligand (RANKL), the OC differentiation factor. Although the roles of ST5 in cancer and ${\beta}-cells$ have been reported, the function of ST5 in bone cells has not yet been investigated. Knockdown of ST5 by siRNA reduced OC differentiation from primary precursors. Moreover, ST5 downregulation decreased expression of NFATc1, a key transcription factor for osteoclastogenesis. In contrast, overexpression of ST5 resulted in the opposite phenotype of ST5 knockdown. In immunocytochemistry experiments, the ST5 protein is colocalized with Src in RANKL-committed cells. In addition, ST5 enhanced activation of Src and Syk, a Src substrate, in response to RANKL. ST5 reduction caused a decrease in RANKL-evoked calcium oscillation and inhibited translocation of NFATc1 into the nucleus. Taken together, these findings provide the first evidence of ST5 involvement in positive regulation of osteoclastogenesis via Src/Syk/calcium signaling.

• 품질경영학회지
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• 제49권3호
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• pp.299-312
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• 2021

Purpose: We provide a condition-based maintenance policy where a surrogate variable is used for monitoring system performance. We constructed a risk function by taking into account the risk and losses accompanied with erroneous decisions. Methods: Assuming a unique degradation process for the performance variable and its specific relationship with the surrogate variable, the maintenance policy is determined. A risk function is developed on the basis of producer's and consumer's risks accompanied with each decision. With a strategic safety factor considered, the optimal threshold value for the surrogate variable is determined based on the risk function. Results: The condition-based maintenance is analyzed from the point of risk. With an assumed safety consideration, the optimal threshold value of the surrogate variable is provided for taking a maintenance action. The optimal solution cannot be obtained in a closed form. An illustrative numerical example and solution is provided with a source code of R program. Conclusion: The study can be applied to situation where a sensor signal is issued if the system performance begins to degrade gradually and reaches eventually its functional failure. The study can be extended to the case where two or more performance variables are connected to a same surrogate variable. Also estimation of the distribution parameters and risk coefficients should be further studied.

• 한국콘텐츠학회논문지
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• 제21권12호
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• pp.842-854
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• 2021

코로나19의 출현 이후 국내외 공연계는 온라인 플랫폼을 활용해 다양한 예술적 실험을 시도하고 있다. 이에 본 연구는 연극 공연을 위한 창작 도구로 활용된 줌(Zoom)의 기능적 특성에 주목하였다. 우선 줌을 활용한 국내외 연극 공연 사례와 그 특징들을 살펴본 후, 줌 연극 <프로젝트, 홍도 Project, Hong-Do>의 제작 단계를 리서치 중심의 프리-프로덕션(Pre-production)단계, 대본을 바탕으로 각 장면을 구성하는 장면 워크숍(Scene Workshop)단계, 장면별 실시간 녹화를 진행하는 레코딩(Recording) 단계와 공연을 상영하기 위한 스트리밍(Streaming) 단계로 구분하여 살펴봄으로써 줌을 통해 창작한 연극의 특성을 고찰할수 있었다. 또한 이러한 제작 과정에서 나타난 배우의 연기 접근 방법을 시선의 분리, 공간의 재인식, 표현적 제스처의 활용, 능동적 행동으로서의 리액션(Reaction)으로 상정하여 살펴보았다. 그 결과 줌을 활용한 연극 작업의 지속적인 발전 가능성과 이에 따른 배우의 연기 접근 방법을 제안할 수 있었다.

• PNF and Movement
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• 제19권1호
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• pp.147-152
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• 2021

Purpose: This study was conducted to investigate the functional differences of the two heads of the biceps brachii by measuring the functions in supination according to pronation by the angle of the flexion of the elbow joint, except for the activities of the elbow flexors. Methods: This study was conducted with 25 healthy men in their 20s. At a glenohumeral-joint 0˚ flexion posture, angles of flexion of the elbow joint of 0˚, 30˚, 60˚, 90˚, and 120˚ were randomly provided, and they were asked to perform supination and pronation with an elastic band. Using an eight-channel surface electromyogram, the muscle activities of the long and short heads of the biceps brachii were measured. The activities of the short and long heads according to the angle were analyzed using a one-way ANOVA, and as a post-test, LSD was employed. Results: The analysis of the impact of the resistance of the elastic band in supination on the differences in the muscle activities in the short and long heads suggests there was a significant difference between 0˚ and 30˚ (p < 0.05). Conclusion: In supination according to forearm pronation, the long head had greater action in the early flexion posture of the elbow joint, and it is judged that the ratio of the flexion of the elbow joint according to the actions of the biceps brachii was considered small.

• Animal Bioscience
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• 제34권1호
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• pp.143-153
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• 2021

Objective: To explore the molecular mechanisms of fatty liver hemorrhagic syndrome (FLHS) in laying hens, an experiment was conducted to reveal the differences in histopathological observation and gene expression between FLHS group and normal group. Methods: We compared the histopathological difference using hematoxylin and eosin staining and proceeded with RNA sequencing of adipose tissue to search differentially expressed genes and enriched biological processes and pathways. Then we validated the mRNA expression levels by real-time polymerase chain reaction and quantified protein levels in the circulation by enzyme-linked immunosorbent assay. Results: We identified 100 differentially expressed transcripts corresponding to 66 genes (DEGs) were identified between FLHS-affected group and normal group. Seven DEGs were significantly enriched in the immune response process and lipid metabolic process, including phospholipase A2 group V, WAP kunitz and netrin domain containing 2, delta 4-desaturase sphingolipid 2, perilipin 3, interleukin-6 (IL-6), ciliary neurotrophic factor (CNTF), and suppressor of cytokine signaling 3 (SOCS3). And these genes could be the targets of immune response and be involved in metabolic homeostasis during the process of FLHS in laying hens. Based on functional categories of the DEGs, we further proposed a model to explain the etiology and pathogenesis of FLHS. IL-6 and SOCS3 mediate inflammatory responses and the satiety hormone of leptin, induce dysfunction of Jak-STAT signaling pathway, leading to insulin resistance and lipid metabolic disorders. Conversely, CNTF may reduce tissue destruction during inflammatory attacks and confer protection from inflammation-induced insulin resistance in FLHS chickens. Conclusion: These findings highlight the therapeutic implications of targeting the JAK-STAT pathway. Inhibition of IL6 and SOCS3 and facilitation of CNTF could serve as a favorable strategy to enhance insulin action and improve glucose homoeostasis, which are of importance for treating obesity-related disorders for chickens.

• 공업화학
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• 제22권5호
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• pp.522-525
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• 2011

본 연구에서는, enzyme-linked immunosorbent assay (ELISA)와 면역크로마토그래픽 기법을 결합하여 Staphylococcus aureus 검출을 위한 면역스트립을 제작하였다. 면역스트립은 4개의 서로 다른 기능을 가진 멤브레인을 이용하여 만들어졌다. 니트로셀룰로오스 멤브레인은 항체와의 결합력이 높기 때문에, 포획항체를 고정화하였고, 다공성 멤브레인들을 통하여 모세관 현상으로 인해 시료흐름을 유도하였다. 효소반응에 의해 생성된 발색신호는 디지털카메라와 자체 제작된 소프트웨어를 이용하여 정성, 정량분석 하였다. 최적의 분석조건 하에서 30 min 이내에 $2.7{\times}10^4{\sim}2.7{\times}10^7CFU/mL$ 범위의 S. aureus 농도를 측정할 수 있었다.

• 동남아시아연구
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• 제28권2호
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• pp.119-145
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• 2018

2017년 창설 50주년을 맞은 아세안(ASEAN)은 제도적 보완과 기능적 협력을 강화하기 위해 일련의 선언과 행동 계획을 채택했다. 아세안의 '회합력'(convening power)이 아세안 중심성(centrality)과 회복력(resilience)을 촉진하고 있다. 반면 아세안 회원국의 민주주의 후퇴는 전통적 규범의 한계를 심화시키고 아세안의 이행력을 저하시킨다. 이 글은 아세안 주요 쟁점을 고찰하고 전망을 도출하고자 한다. 첫째, 아세안의 제도적 발전과 기능적 협력의 현황을 아세안 공동체 건설의 현황과 쟁점을 중심으로 살피고자 한다. 둘째, 아세안의 대외 관계를 중국, 미국, 한국과의 관계를 중심으로 살피고자 한다. 남중국해 이슈 및 중국의 경제적 영향력 확대는 아세안-중국관계의 복잡성을 심화시키고 있다. 트럼프 행정부의 대 동남아 정책 변화에 대응하기 위해서는 아세안의 단결이 필요하다. 한국의 신남방정책은 여러 제약에도 불구하고 양자 관계에 있어 전환점이 될 것으로 기대된다. 대외 환경의 변화 속에 아세안 단결과 중심성의 강화가 더욱 절실해지고 있다.

• 한국정보통신학회논문지
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• 제26권6호
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• pp.850-858
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• 2022

리소스가 제한된 임베디드 장치에 GRU를 배포하기 위해 이 논문은 구조적 압축을 가능하게 하는 재구성 가능한 FPGA 기반 GRU 가속기를 설계한다. 첫째, 조밀한 GRU 모델은 하이브리드 양자화 방식과 구조화된 top-k 프루닝에 의해 크기가 대폭 감소한다. 둘째, 본 연구에서 제시하는 재사용 컴퓨팅 패턴에 의해 외부 메모리 액세스에 대한 에너지 소비가 크게 감소한다. 마지막으로 가속기는 알고리즘-하드웨어 공동 설계 워크플로의 이점을 얻는 구조화된 희소 GRU 모델을 처리할 수 있다. 또한 모든 차원, 시퀀스 길이 및 레이어 수를 사용하여 GRU 모델에 대한 추론 작업을 유연하게 수행할 수 있다. Intel DE1-SoC FPGA 플랫폼에 구현된 제안된 가속기는 일괄 처리가 없는 구조화된 희소 GRU 네트워크에서 45.01 GOPs를 달성하였다. CPU 및 GPU의 구현과 비교할 때 저비용 FPGA 가속기는 대기 시간에서 각각 57배 및 30배, 에너지 효율성에서 300배 및 23.44배 향상을 달성한다. 따라서 제안된 가속기는 실시간 임베디드 애플리케이션에 대한 초기 연구로서 활용, 향후 더 발전될 수 있는 잠재력을 보여준다.

• Animal Bioscience
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• 제36권2호
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• pp.175-190
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• 2023

Objective: The study was conducted to screen differentially expressed long noncoding RNA (lncRNA) in chickens by high-throughput sequencing and explore its mechanism of action on intramuscular fat deposition. Methods: Herein, Rose crown and Cbb broiler chicken embryo breast and leg muscle lncRNA and mRNA expression profiles were constructed by RNA sequencing. A total of 96 and 42 differentially expressed lncRNAs were obtained in Rose crown vs Cobb broiler chicken breast and leg muscle, respectively. lncRNA-ENSGALT00000046546, with high interspecific variability and a potential regulatory role in lipid metabolism, and its predicted downstream target gene 1-acylglycerol-3-phosphate-O-acyltransferase 2 (AGPAT2), were selected for further study on the preadipocytes. Results: lncRNA-46546 overexpression in chicken preadipocyte 2 cells significantly increased (p<0.01) the expression levels of AGPAT2 and its downstream genes diacylglycerol acyltransferase 1 and diacylglycerol acyltransferase 2 and those of the fat metabolism-related genes peroxisome proliferator-activated receptor γ, CCAAT/enhancer binding protein α, fatty acid synthase, sterol regulatory element-binding transcription factor 1, and fatty acid binding protein 4. The lipid droplet concentration was higher in the overexpression group than in the control cells, and the triglyceride content in cells and medium was also significantly increased (p<0.01). Conclusion: This study preliminarily concludes that lncRNA-46546 may promote intramuscular fat deposition in chickens, laying a foundation for the study of lncRNAs in chicken early embryonic development and fat deposition.

• 대한본초학회지
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• 제28권2호
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• pp.55-60
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• 2013

Objectives : Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by eczematous inflammtion of the skin. The chestnut inner shell extracts (CI) has been used as a cosmetic material for a long time in Korea. However, the precise anti-allergy effects of CI have yet to be clearly elucidated. In this study, we attempted to evaluate the effect of CI on mast cell-mediated allergy inflammation. Methods : To find the anti-allergy and inflammatory effect of CI, we investigated the inhibitory effect of CI on the production of inflammatory mediators using by enzyme-linked immunosorbent assay in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore (A23187) stimulated-human mast cell (HMC-1). Results : In this study, we found that CI did not show cytotoxic effect at up to 10 ug/ml on HMC-1. CI inhibited the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6 and IL-8 in stimulated HMC-1. Maximal rate of TNF-${\alpha}$, IL-6 and IL-8 inhibition by CI (10 ug/ml) were about 47.6%, 44.1% and 22.5% respectively. In addition, we showed that Fr.3 isolated from n-Butyl alcohol layer of CI attenuated the production of TNF-${\alpha}$, IL-6 and IL-8 in HMC-1. Conclusion : Taken together, the findings of this study provide us with a novel insight action of CI as a potential molecule for use in the treatment of allergic inflammation diseases.