• Title/Summary/Keyword: acp

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Effect of three nanobiomaterials on microhardness of bleached enamel

  • Khoroushi, Maryam;Shirban, Farinaz;Kaveh, Sara;Doustfateme, Samaneh
    • Restorative Dentistry and Endodontics
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    • v.41 no.3
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    • pp.196-201
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    • 2016
  • Objectives: The aim of this in vitro study was to evaluate the effect of incorporating three different nanobiomaterials into bleaching material on microhardness of bleached enamel. Materials and Methods: The crowns of 24 extracted sound human molars were sectioned. Sixty enamel specimens ($2{\times}3{\times}4 mm$) were selected and divided into five groups (n = 12): Group 1 received no bleaching procedure (control); Group 2 underwent bleaching with a 40% hydrogen peroxide (HP) gel; Groups 3, 4, and 5 were bleached with a 40% HP gel modified by incorporation of bioactive glass (BAG), amorphous calcium phosphate (ACP) and hydroxyapatite (HA), respectively. The enamel microhardness was evaluated. The differences in Knoop microhardness data of each group were analyzed by one-way ANOVA, followed by post hoc Tukey tests. Results: Significant differences were observed between the study groups. The enamel microhardness changes in Groups 1, 3, 4, and 5 were significantly lower than that of Group 2 (p < 0.001). Conclusions: Within the limitations of this study, it can be concluded that incorporation of each one of the three tested biomaterials as remineralizing agents might be effective in decreasing enamel microhardness changes subsequent to in-office bleaching.

Flip Chip Interconnection Method Applied to Small Camera Module

  • Segawa, Masao;Ono, Michiko;Karasawa, Jun;Hirohata, Kenji;Aoki, Makoto;Ohashi, Akihiro;Sasaki, Tomoaki;Kishimoto, Yasukazu
    • Proceedings of the International Microelectronics And Packaging Society Conference
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    • 2000.10a
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    • pp.39-45
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    • 2000
  • A small camera module fabricated by including bare chip bonding methods is utilized to realize advanced mobile devices. One of the driving forces is the TOG (Tape On Glass) bonding method which reduces the packaging size of the image sensor clip. The TOG module is a new thinner and smaller image sensor module, using flip chip interconnection method with the ACP (Anisotropic Conductive Paste). The TOG production process was established by determining the optimum bonding conditions for both optical glass bonding and image sensor clip bonding lo the flexible PCB. The bonding conditions, including sufficient bonding margins, were studied. Another bonding method is the flip chip bonding method for DSP (Digital Signal Processor) chip. A new AC\ulcorner was developed to enable the short resin curing time of 10 sec. The bonding mechanism of the resin curing method was evaluated using FEM analysis. By using these flip chip bonding techniques, small camera module was realized.

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Estimation of Atmospheric PAH Concentrations and Sources at Several Sites Using Pine Needles as a Passive Air Sampler (소나무잎을 Passive Air Sampler로 이용하여 지역별 대기 중 다환방향족 탄화수소의 농도 및 발생원 추정)

  • Chun, Man-Young
    • Journal of Environmental Health Sciences
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    • v.40 no.1
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    • pp.38-46
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    • 2014
  • Objectives: This study was carried out in order to estimate atmospheric polycyclic aromatic hydrocarbon (PAH) concentrations and sources using pine needles as a passive air sampler (PAS) in urban (Pyeongtaek), semirural (Anseong) and rural (Jincheon) sites. Methods: One-year-old pine needles were collected for analysis of their PAH concentrations ($C_{p,n}g/g$ dry) at the end of December. PAHs concentrations in the ambient air ($C_a$, $ng/m^3$) were calculated with a $Log(C_p/C_a)-LogK_{oa}$ correlational equation. Results: PAHs concentrations in ambient air ($C_a$) were high, in the order of urban ($114.03ng/m^3$), semirural ($105.17ng/m^3$) and rural ($61.91ng/m^3$) sites. However, distributions of PAH isomer concentrations were very similar. PAHs of which molecular weight is smaller than 228.30 (AcPy, Acp, Flu, Phen, Ant, Flt, Pyr, BaA, Chry) made up most of the PAHs in the ambient air (96.6-98.5%). Conclusion: At urban, semirural and rural sites, it was concluded that the main source of PAHs in the ambient air ratio of each PAH isomer concentration was cars, especially diesel vehicles.

Identification of Differentially Expressed Genes (DEGs) by Malachite Green in HepG2 Cells

  • Kim, Youn-Jung;Song, Mee;Ryu, Jae-Chun
    • Molecular & Cellular Toxicology
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    • v.4 no.1
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    • pp.22-30
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    • 2008
  • Malachite Green (MG), a toxic chemical used as a dye, topical antiseptic and antifungal agent for fish, is highly soluble in water, cytotoxic to various mammalian cells and also acts as a liver tumor promoter. In view of its industrial importance and possible exposure to human beings, MG possesses a potential environmental health hazard. So, we performed with HepG2, a human hepatocellular carcinoma cell line, to identify the differentially expressed genes (DEGs) related to toxicity of MG. And we compared gene expression between control and MG treatment to identify genes that are specifically or predominantly expressed by employing annealing control primer (ACP)-based $GeneFishing^{TM}$ method. The cytotoxicity $(IC_{20})$ of MG was determined above the $0.867{\mu}M$ in HepG2 cell for 48 h treatment. And the DEGs of MG were identified that 5 out of 6 DEGs were upregulated and 1 out of 6 DEGs was down-regulated by MG. Also, MG induced late apoptosis and necrosis in a dose dependent in flow cytometric analysis. Through further investigation, we will identify more meaningful and useful DEGs on MG, and then can get the information on mechanism and pathway associated with toxicity of MG.

A test of Visual Acuity and Refractive error to Kindergarten's Children (유치원 어린이 나안시력과 굴절이상 측정)

  • Kang, I.S.
    • Journal of Korean Ophthalmic Optics Society
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    • v.3 no.1
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    • pp.87-92
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    • 1998
  • Unaided visual acuity was tested by ACP-7 TOPCON chart projector on 376 kindergarteners and objective refraction error was measured by NIDEK ARK-700A auto-refractokeratometer on 554 eyes aged 3 to 5. The results were as follows ; The average unaided visual acuity of children aged 3 was 0.82, aged 4 was 0.90 and aged 5 was 0.92 respectively theerfore children s visual acuity has been gradually developed with their age. The kind of refractive error was 1% for hyperopia, 14% for hyperopic astigmatism, 3% for myopia, 50% for myopic astigmatism, 18% for mixed astigmatism and 14% for emmetropia respectively.

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Development and Performance Tests of the Bridge-Transported Servo Manipulator System for Remote Maintenance Jobs in a Hotcell

  • Jin Jaehyun;Park Byungsuk;Ko Byungseung;Yoon Jisup;Jung Ki-Jung
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.3 no.3
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    • pp.257-268
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    • 2005
  • In this paper, a prototype of the Bridge-Transported Servo Manipulator (BTSM) system is introduced, which has been developed to do operation and maintenance jobs remotely in a hot cell. The system consists of a telescopic transporter, a slave arm, a master arm, and a control system. Several tests such as a positional tracking, a weight handling, reliability, and operability have been performed and test results are presented. Based on the test results, an upgraded system which will be used during demonstrations of the advanced spent fuel conditioning process (ACP) has been designed.

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Characterization and cDNA Cloning of a Defensin-Like Peptide, Harmoniasin, from Harmonia axyridis

  • Kim, In-Woo;Lee, Joon-Ha;Park, Ha-Yan;Kwon, Young-Nam;Yun, Eun-Young;Nam, Sung-Hee;Kim, Seong-Ryul;Ahn, Mi-Young;Hwang, Jae Sam
    • Journal of Microbiology and Biotechnology
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    • v.22 no.11
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    • pp.1588-1590
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    • 2012
  • We compared the mRNA expression profile of the Harmonia axyridis larvae that were either untreated or treated with LPS. The extracted mRNAs were subjected to ACP RT-PCR analysis using a combination of arbitrary primers and oligo (dT) primer. Among the 47 DEGs differentially expressed, we identified a cDNA showing homology with defensin-like antibacterial peptide. The cDNA showed a putative 32-residue signal sequence and a 50-residue mature peptide named harmoniasin. We also investigated the antibacterial activity of the harmoniasin analog, which exhibited potent antibacterial activities against Gramnegative and -positive bacteria strains and it also evidenced no hemolytic activity.

Different Effects of Acidic pH Shock on the Prodiginine Production in Streptomyces coelicolor M511 and SJM1 Mutants

  • Mo, SangJoon;Kim, Jae-Heon;Oh, Chung-Hun
    • Journal of Microbiology and Biotechnology
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    • v.23 no.10
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    • pp.1454-1459
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    • 2013
  • The changes in prodiginines productions caused by pH shock culture of Streptomyces coelicolor strains were estimated. In Streptomyces coelicolor M511, undecylprodiginine and streptorubin B productions increased 1.8-fold (37.22 mg/g) and 2.5-fold (18.61 mg/g), respectively, by pH shock (from 7.2 to 4.0). In contrast, this resulted in the significantly decreased prodigignines production in the redP deletion mutant SJM1; 3.7-fold for undecylprodiginine, 4.4-fold for streptorubin B, 5.2-fold for methylundecylprodiginine, and 6.4-fold for methyldodecylundecylprodiginine, respectively. RT-PCR analyses showed that, during pH shock, expression of redD, the transcription activator gene, was increased while the expression of fabH, the decarboxylative condensation enzyme gene in fatty acid biosynthesis, was decreased in both strains. The enhanced redD expression was in good accordance with the increased total prodiginines production of M511. However, for SJM1 mutant, the decrease of fabH expression occurred more strikingly, such that it became almost completely turned off during acidic pH shock culture. Therefore, a down-regulation of fabH was considered to be the cause of decreased amount of total prodiginines produced, although redD expression was high in SJM1 mutant.

${\beta}$-ketoacyl-acyl carrier protein synthases for fatty acid biosynthesis in bacteria

  • Lee, Hee-Jung;Youn, Youn-Ji;Ok, Jung-In;Lee, Jung-Won;Park, Hyo-Young;Cho, Kyung-Hae;Choi, Keum-Hwa
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.315.3-316
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    • 2002
  • A universal set of genes encodes the components of dissociated. type II. fa11y acid synthase system that is responsible for producing the multitude of fa11y acid structures found in bacterial membranes. We examined the biochemical basis for the production of fatty acids by bacteria. Several genes from HaemophHus influenzae Rd and three genes from Enterococcus faecalis V583 were predicted to encode homologs of the ${\beta}$-ketoacyl-acyl carrier protein synthases I or II or III of Escherichia coli(FabB or BabF, or FabH)were identified in the genomic database. The protein products were expressed. purified, and biochemically characterized. efFabH and hF abH carried out the initial condensation reaction of fatty acid biosynthesis with acetyl-Coenzyme A as a primer. and hFabB and efFabF1 carried out the elongation condensation reaction of fatty acid biosynthesis with myrixtoyl-ACP.

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AN EXPERIMENTAL STUDY ON AN ELECTROCHEMICAL REDUCTION OF AN OXIDE MIXTURE IN THE ADVANCED SPENT-FUEL CONDITIONING PROCESS

  • Jeong, Sang-Mun;Park, Byung-Heung;Hur, Jin-Mok;Seo, Chung-Seok;Lee, Han-Soo;Song, Kee-Chan
    • Nuclear Engineering and Technology
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    • v.42 no.2
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    • pp.183-192
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    • 2010
  • An electrochemical reduction of a mixture of metal oxides was conducted in a LiCl molten salt containing 3 wt% $Li_2O$ at $650^{\circ}C$. The oxide reduction was carried out by applying a current to an electrolysis cell, and the $Li_2O$ concentration was analyzed during each run. The concentration of $Li_2O$ in the electrolyte bulk phase gradually decreases according to Faraday's law due to a slow diffusion of the $O^{2-}$ ions. A hindrance effect of the unreduced metal oxides was observed for the reduction of the uranium oxide. Cs, Sr, and Ba of high heat-load fission products were diffused into and accumulated in the salt phase as predicted with thermodynamic consideration.