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Effect of three nanobiomaterials on microhardness of bleached enamel

  • Khoroushi, Maryam (Dental Materials Research Center, Department of Operative Dentistry) ;
  • Shirban, Farinaz (Dental Research Center and Department of Orthodontics, School of Dentistry, Isfahan University of Medical Sciences) ;
  • Kaveh, Sara (Department of Operative Dentistry, School of Dentistry, Semnan University of Medical Sciences) ;
  • Doustfateme, Samaneh (Persian Gulf Oral and Dental Disease Research Center, Hormozgan University of Medical Sciences)
  • Received : 2016.02.23
  • Accepted : 2016.05.30
  • Published : 2016.08.31

Abstract

Objectives: The aim of this in vitro study was to evaluate the effect of incorporating three different nanobiomaterials into bleaching material on microhardness of bleached enamel. Materials and Methods: The crowns of 24 extracted sound human molars were sectioned. Sixty enamel specimens ($2{\times}3{\times}4 mm$) were selected and divided into five groups (n = 12): Group 1 received no bleaching procedure (control); Group 2 underwent bleaching with a 40% hydrogen peroxide (HP) gel; Groups 3, 4, and 5 were bleached with a 40% HP gel modified by incorporation of bioactive glass (BAG), amorphous calcium phosphate (ACP) and hydroxyapatite (HA), respectively. The enamel microhardness was evaluated. The differences in Knoop microhardness data of each group were analyzed by one-way ANOVA, followed by post hoc Tukey tests. Results: Significant differences were observed between the study groups. The enamel microhardness changes in Groups 1, 3, 4, and 5 were significantly lower than that of Group 2 (p < 0.001). Conclusions: Within the limitations of this study, it can be concluded that incorporation of each one of the three tested biomaterials as remineralizing agents might be effective in decreasing enamel microhardness changes subsequent to in-office bleaching.

Keywords

References

  1. Kihn PW. Vital tooth whitening. Dent Clin North Am 2007;51:319-331. https://doi.org/10.1016/j.cden.2006.12.001
  2. Borges AB, Yui KC, D'Avila TC, Takahashi CL, Torres CR, Borges AL. Influence of remineralizing gels on bleached enamel microhardness in different time intervals. Oper Dent 2010;35:180-186. https://doi.org/10.2341/09-117-L
  3. Justino LM, Tames DR, Demarco FF. In situ and in vitro effects of bleaching with carbamide peroxide on human enamel. Oper Dent 2004;29:219-225.
  4. Sulieman M, Addy M, Macdonald E, Rees JS. A safety study in vitro for the effects of an in-office bleaching system on the integrity of enamel and dentine. J Dent 2004;32:581-590. https://doi.org/10.1016/j.jdent.2004.06.003
  5. Maia E, Baratieri LN, Caldeira de Andrada MA, Monteiro S Jr, Vieira LC. The influence of two home-applied bleaching agents on enamel microhardness: an in situ study. J Dent 2008;36:2-7.
  6. Cadenaro M, Breschi L, Nucci C, Antoniolli F, Visintini E, Prati C, Matis BA, Di Lenarda R. Effect of two inoffice whitening agents on the enamel surface in vivo: a morphological and non-contact profilometric study. Oper Dent 2008;33:127-134. https://doi.org/10.2341/07-89
  7. McCracken MS, Haywood VB. Demineralization effects of 10 percent carbamide peroxide. J Dent 1996;24:395-398. https://doi.org/10.1016/0300-5712(95)00113-1
  8. Tezel H, Ertas OS, Ozata F, Dalgar H, Korkut ZO. Effect of bleaching agents on calcium loss from the enamel surface. Quintessence Int 2007;38:339-347.
  9. Al-Salehi SK, Wood DJ, Hatton PV. The effect of 24h non-stop hydrogen peroxide concentration on bovine enamel and dentine mineral content and microhardness. J Dent 2007;35:845-850. https://doi.org/10.1016/j.jdent.2007.08.001
  10. Jiang T, Ma X, Wang Y, Tong H, Shen X, Hu Y, Hu J. Investigation of the effects of 30% hydrogen peroxide on human tooth enamel by Raman scattering and laserinduced fluorescence. J Biomed Opt 2008;13:014019. https://doi.org/10.1117/1.2870114
  11. Rodrigues JA, Marchi GM, Ambrosano GM, Heymann HO, Pimenta LA. Microhardness evaluation of in situ vital bleaching on human dental enamel using a novel study design. Dent Mater 2005;21:1059-1067. https://doi.org/10.1016/j.dental.2005.03.011
  12. Basting RT, Rodrigues AL Jr, Serra MC. The effects of seven carbamide peroxide bleaching agents on enamel microhardness over time. J Am Dent Assoc 2003;134:1335-1342. https://doi.org/10.14219/jada.archive.2003.0047
  13. Cavalli V, Giannini M, Carvalho RM. Effect of carbamide peroxide bleaching agents on tensile strength of human enamel. Dent Mater 2004;20:733-739. https://doi.org/10.1016/j.dental.2003.10.007
  14. Attin T, Vollmer D, Wiegand A, Attin R, Betke H. Subsurface microhardness of enamel and dentin after different external bleaching procedures. Am J Dent 2005;18:8-12.
  15. Lewinstein I, Fuhrer N, Churaru N, Cardash H. Effect of different peroxide bleaching regimens and subsequent fluoridation on the hardness of human enamel and dentin. J Prosthet Dent 2004;92:337-342. https://doi.org/10.1016/j.prosdent.2004.07.019
  16. Park HJ, Kwon TY, Nam SH, Kim HJ, Kim KH, Kim YJ. Changes in bovine enamel after treatment with a 30% hydrogen peroxide bleaching agent. Dent Mater J 2004;23:517-521. https://doi.org/10.4012/dmj.23.517
  17. Deng M, Wen HL, Dong XL, Li F, Xu X, Li H, Li JY, Zhou XD. Effects of 45S5 bioglass on surface properties of dental enamel subjected to 35% hydrogen peroxide. Int J Oral Sci 2013;5:103-110. https://doi.org/10.1038/ijos.2013.31
  18. Severcan F, Gokduman K, Dogan A, Bolay S, Gokalp S. Effects of in-office and at-home bleaching on human enamel and dentin: an in vitro application of Fourier transform infrared study. Appl Spectrosc 2008;62:1274-1279. https://doi.org/10.1366/000370208786401554
  19. da Silva AP, de Oliveira R, Cavalli V, Arrais CA, Giannini M, de Carvalho RM. Effect of peroxide-based bleaching agents on enamel ultimate tensile strength. Oper Dent 2005;30:318-324.
  20. Joiner A. The bleaching of teeth: a review of the literature. J Dent 2006;34:412-419. https://doi.org/10.1016/j.jdent.2006.02.002
  21. Jiang T, Ma X, Wang Z, Tong H, Hu J, Wang Y. Beneficial effects of hydroxyapatite on enamel subjected to 30% hydrogen peroxide. J Dent 2008;36:907-914. https://doi.org/10.1016/j.jdent.2008.07.005
  22. Borges BC, Borges JS, de Melo CD, Pinheiro IV, Santos AJ, Braz R, Montes MA. Efficacy of a novel at-home bleaching technique with carbamide peroxides modified by CPP-ACP and its effect on the microhardness of bleached enamel. Oper Dent 2011;36:521-528. https://doi.org/10.2341/11-013-L
  23. da Costa JB, Mazur RF. Effects of new formulas of bleaching gel and fluoride application on enamel microhardness: an in vitro study. Oper Dent 2007;32:589-594. https://doi.org/10.2341/06-166
  24. Chen HP, Chang CH, Liu JK, Chuang SF, Yang JY. Effect of fluoride containing bleaching agents on enamel surface properties. J Dent 2008;36:718-725. https://doi.org/10.1016/j.jdent.2008.05.003
  25. Burgmaier GM, Schulze IM, Attin T. Fluoride uptake and development of artificial erosions in bleached and fluoridated enamel in vitro. J Oral Rehabil 2002;29:799-804. https://doi.org/10.1046/j.1365-2842.2002.00966.x
  26. Tschoppe P, Neumann K, Mueller J, Kielbassa AM. Effect of fluoridated bleaching gels on the remineralization of predemineralized bovine enamel in vitro. J Dent 2009;37:156-162. https://doi.org/10.1016/j.jdent.2008.11.001
  27. Kanemura N, Sano H, Tagami J. Tensile bond strength to and SEM evaluation of ground and intact enamel surfaces. J Dent 1999;27:523-530. https://doi.org/10.1016/S0300-5712(99)00008-1
  28. Faraoni-Romano JJ, da Silveira AG, Turssi CP, Serra MC. Bleaching agents with varying concentrations of carbamide and/or hydrogen peroxides: effect on dental microhardness and roughness. J Esthet Restor Dent 2008;20:395-402. https://doi.org/10.1111/j.1708-8240.2008.00216.x
  29. Soldani P, Amaral CM, Rodrigues JA. Microhardness evaluation of in situ vital bleaching and thickening agents on human dental enamel. Int J Periodontics Restor Dent 2010;30:203-211.
  30. Turkun M, Sevgican F, Pehlivan Y, Aktener BO. Effects of 10% carbamide peroxide on the enamel surface morphology: a scanning electron microscopy study. J Esthet Restor Dent 2002;14:238-244. https://doi.org/10.1111/j.1708-8240.2002.tb00169.x
  31. Featherstone JD, ten Cate JM, Shariati M, Arends J. Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res 1983;17:385-391. https://doi.org/10.1159/000260692
  32. Cunha AG, de Vasconcelos AA, Borges BC, Vitoriano Jde O, Alves-Junior C, Machado CT, dos Santos AJ. Efficacy of in-office bleaching techniques combined with the application of a casein phosphopeptide-amorphous calcium phosphate paste at different moments and its influence on enamel surface properties. Microsc Res Tech 2012;75:1019-1025. https://doi.org/10.1002/jemt.22026
  33. Attin T, Schmidlin PR, Wegehaupt F, Wiegand A. Influence of study design on the impact of bleaching agents on dental enamel microhardness: a review. Dent Mater 2009;25:143-157. https://doi.org/10.1016/j.dental.2008.05.010
  34. de Vasconcelos AA, Cunha AG, Borges BC, Machado CT, dos Santos AJ. Tooth whitening with hydrogen/carbamide peroxides in association with a CPP-ACP paste at different proportions. Aust Dent J 2012;57:213-219. https://doi.org/10.1111/j.1834-7819.2012.01683.x
  35. Niwa M, Sato T, Li W, Aoki H, Aoki H, Daisaku T. Polishing and whitening properties of toothpaste containing hydroxyapatite. J Mater Sci Mater Med 2001;12:277-281.
  36. Generosi A, Rau JV, Rossi Albertini V, Paci B. Crystallization process of carbonate substituted hydroxyapatite nanoparticles in toothpastes upon physiological conditions: an in situ time-resolved X-ray diffraction study. J Mater Sci Mater Med 2010;21:445-450.
  37. Curtis AR, West NX, Su B. Synthesis of nanobioglass and formation of apatite rods to occlude exposed dentine tubules and eliminate hypersensitivity. Acta Biomater 2010;6:3740-3746. https://doi.org/10.1016/j.actbio.2010.02.045
  38. Rehder Neto FC, Maeda FA, Turssi CP, Serra MC. Potential agents to control enamel caries-like lesions. J Dent 2009;37:786-790. https://doi.org/10.1016/j.jdent.2009.06.008
  39. Burwell AK, Litkowski LJ, Greenspan DC. Calcium sodium phosphosilicate (NovaMin): remineralization potential. Adv Dent Res 2009;21:35-39. https://doi.org/10.1177/0895937409335621
  40. Andersson OH, Kangasniemi I. Calcium phosphate formation at the surface of bioactive glass in vitro. J Biomed Mater Res 1991;25:1019-1030. https://doi.org/10.1002/jbm.820250808
  41. Chow LC, Sun L, Hockey B. Properties of nanostructured hydroxyapatite prepared by a spray drying technique. J Res Nati Inst Stand Technol 2004;109:543-551. https://doi.org/10.6028/jres.109.041
  42. Onuma K, Yamagishi K, Oyane A. Nucleation and growth of hydroxyapatite nanocrystals for nondestructive repair of early caries lesions. J Crys Gro 2005;282:199-207. https://doi.org/10.1016/j.jcrysgro.2005.04.085
  43. Vano M, Derchi G, Barone A, Genovesi A, Covani U. Tooth bleaching with hydrogen peroxide and nanohydroxyapatite: a 9-month follow-up randomized clinical trial. Int J Dent Hyg 2015;13:301-307. https://doi.org/10.1111/idh.12123
  44. Walker GD, Cai F, Shen P, Adams GG, Reynolds C, Reynolds EC. Casein phosphopeptide-amorphous calcium phosphate incorporated into sugar confections inhibits the progression of enamel subsurface lesions in situ. Caries Res 2010;44:33-40. https://doi.org/10.1159/000275572
  45. Khoroushi M, Shirban F, Doustfateme S, Kaveh S. Effect of three nanobiomaterials on the surface roughness of bleached enamel. Contemp Clin Dent 2015;6:466-470. https://doi.org/10.4103/0976-237X.169853

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