• The Korean Journal of Physiology and Pharmacology
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• 제3권5호
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• pp.521-528
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• 1999

Reactive oxygen species (ROS), generated by infiltrating neutrophils, are considered as an important regulator in the pathogenesis and deveolpment of pancreatitis. The present study aims to investigate whether neutrophils primed by $4{\beta}-phorbol\;12{\beta}-myristate\;13{\alpha}-acetate$ (PMA) affect the productions $H_2O_2$ and lipid peroxide (LPO), $NF-_{\kappa}B$ activation and cytokine production in pancreatic acinar cells, and whether these alterations were inhibited by an antioxidant, N-acetylcysteine (NAC) and superoxide dismutase (SOD). $H_2O_2$ (ferrithiocyanate method), LPO (as thiobarbiturate reactive substances), and cytokines $(IL-l{\bata},\; IL-6,\;TNF-{\alpha};\;enzyme-linked\;immunosorbent\;assay)$ and $NF-_{\kappa}B$ activation (electrophoretic mobility shift assay) were analyzed in acinar cells treated with or without PMA-primed neutrophils in the absence or presence of NAC (10 mM) or SOD (300 U/ml). As a result, the productions of H2O2, LPO and $TNF-{\alpha}$ were increased with the ratio of PMA-primed neutrophils to acinar cells while the productions of LPO, $IL-l{\beta},\;IL-6\;and\;TNF-{\alpha}$ were increased with time. PMA-primed neutrophils resulted in the activation of $NF-_{\kappa}B.$ Both NAC and SOD inhibited neutrophil-induced alterations in acinar cells. In conclusion, ROS, generated by neutrophils, activates $NF-_{\kappa}B,$ resulting in upregulation of inflammatary cytokines in acinar cells. Antioxidants might be clinically useful antiinflammatory agents by inhibiting oxidant-mediated activation of $NF-_{\kappa}B$ and decreasing cytokine production.

• Journal of Nutrition and Health
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• 제19권6호
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• pp.374-381
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• 1986

CCK ( Cholecystokinin)와 carbachol (carbamylchline chloride)이 췌장의 효소분비능력을 촉진시킨다는 사실은 잘 알려져있다. 생대두식이를 먹인 쥐에서 췌장세포의 hypertrophy 현상이 두드러지게 나타나고 소화효소의 과다분비 현상이 나타나므로 이번 실험에서는 익힌 대두와 생대두를 먹인 후 정상적인 췌장세포와 hypertrophy 가 일어난 췌장세포에 CCK 와 carbachol 로 자극을 준후 효소분비 능력을 비교하였다. 췌장세포에서 분해되는 단백질 분해효소들이 세포자체에 미치는 영향을 최소한으로 줄이기 위하여 췌장의 acinar cell을 분리한 후 cell column을 만들어 superfusion technique 에 의해 stimulus-enzyme secretion coupling 방법을 사용하였다. 분리한 췌장세포를 CCK와 carbachol 로 자극을 주었을대 chymotrysin 분비는 생대두를 먹인 쥐의 세포에서 익힌 대두를 먹인 군보다 높게 나타났고 amylase의 분비는 chymotrypsin 과는 달리 익힌 대두를 먹인 쥐의 세포에서 생대두를 먹인 군보다 높게 나타났다. 이번 실험의 결과는 지금까지 논쟁중에 있는 췌장의 acinar cell에서의 여러 가지 효소분비 비율은 항상 일정한 것이 아니라 자극의 종류에 따라 효소분비량의 구성비율이 달라진다는 것을 알려주고 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제11권4호
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• pp.428-433
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• 1998

The effects of chromium picolinate supplementation in pig diet were evaluated by measuring the in vitro lipogenic and lipolytic activities in adipose tissue and the protein synthetic activity in liver acinar cell in culture. Thirty-two male and thirty-two female pigs were randomly assigned to one of four dietary groups: Control, 100 ppb, 200 ppb, and 400 ppb of Cr in the form of picolinate. The chromium picolinate supplementation (p < 0.01) increased the in vitro lipolytic activity in adipose tissue of pig, but had no effects on lipogenesis. The chromium picolinate effect was greater in female pigs than in male pigs on lipolytic activity. The results from the studies with the liver acinar cells in culture indicated that chromium picolinate supplementation increased protein synthetic activity (p < 0.05). It was observed through this experiment that chromium picolinate functions not only on fat degradation but also on retained protein synthesis.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.56-56
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• 2003

Isolated single pancreatic acinar cells have long been used as a good model for studying many kinds of signaling processes due to their good structural and functional polarities without a significant validation. In this study, we have examined morphological and functional changes of the dissociated single pancreatic acinar cells by imaging cytosolic Ca$\^$2+/ concentration, exocytosis of granules, and by observing their shapes with confocal microscopy.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2001년도 학술 발표회 진행표 및 논문초록
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• pp.27-27
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• 2001

In exocrine acinar cells, $Ca^{2＋}$ -activated Cl$^{[-10]}$ channels in the apical membrane are essential for fluid secretion, but it is unclear whether such channels are important for Cl$^{[-10]}$ uptake at the base. Whole cell current recording, combined with local uncaging of caged $Ca^{2＋}$, was used to reveal the Cl$^{[-10]}$ channel distribution in mouse pancreatic acinar cells, where ~90％ of the current activated by $Ca^{2＋}$ in response toacetylcholine was carried by Cl$^{[-10]}$ .(omitted)

• Applied Microscopy
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• 제35권4호
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• pp.91-97
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• 2005

이하선의 선세포와 분비과립을 작은땃쥐 Crocidura suaveolens와 우수리땃쥐 C. lasiura에서 연구하였다. 두 종 모두의 이하선은 장액선세포 한 종류만을 가지는 장액선이었고 선세포, 사이관, 과립관 그리고 줄무늬관들은 평범하였다. 작은땃쥐의 경우, 장액선세포는 잘 발달된 조면소포체, 현저한 골지체, 몇몇의 큰 미토콘드리아와 성숙 혹은 융합하고 있는 많은 중간 정도의 전자 밀도 과립들을 가지고 있었다. 미성숙 선세포의 분비과립들은 미세하고 강한 전자 밀도의 알갱이만으로 혹은 알갱이들이 주가 되었고 불분명한 경계막을 가지고 있었고, 성숙 분비과립들은 균일하고 옅은 중심부를 가지고 그 주변에 미세하고 강한 전자밀도의 알갱이들과 명확한 경계막을 가지고 있었다. 우수리땃쥐의 경우, 선세포는 잘 발달된 조면소포체, 현저한 골지체, 몇몇의 큰 미토콘드리아 뿐 아니라 성숙 혹은 융합하고 있는 많은 진한 전자 밀도의 분비과립들을 가지고 있었다. 미성숙 선세포 분비과립들은 옅고 거친 알갱이들만으로 채워져 있었으며 불명확한 경계막을 가지고 있었고, 성숙 분비과립들은 진하고 거친 알갱이들만으로 채워져 있었고 명확한 경계막을 가지고 있었다. 결국, 작은땃쥐의 이하선 분비과립은 광학과 전자현미경 수준에서 중간 정도의 전자밀도로, 우수리땃쥐의 분비과립은 광학 현미경 수준에서는 아주 진하게, 전자현미경에서는 진하게 관찰되었다.

• Molecules and Cells
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• 제19권2호
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• pp.268-278
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• 2005

We investigated the effect of cytosolic and extracellular $Ca^{2+}$ on $Ca^{2+}$ signals in pancreatic acinar cells by measuring $Ca^{2+}$ concentration in the cytosol($[Ca^{2+}]_c$) and in the lumen of the ER($[Ca^{2+}]_{Lu}$). To control buffers and dye in the cytosol, a patch-clamp microelectrode was employed. Acetylcholine released $Ca^{2+}$ mainly from the basolateral ER-rich part of the cell. The rate of $Ca^{2+}$ release from the ER was highly sensitive to the buffering of $[Ca^{2+}]_c$ whereas ER $Ca^{2+}$ refilling was enhanced by supplying free $Ca^{2+}$ to the cytosol with $[Ca^{2+}]_c$ clamped at resting levels with a patch pipette containing 10 mM BAPTA and 2 mM $Ca^{2+}$. Elevation of extracellular $Ca^{2+}$ to 10 mM from 1 mM raised resting $[Ca^{2+}]_c$ slightly and often generated $[Ca^{2+}]_c$ oscillations in single or clustered cells. Although pancreatic acinar cells are reported to have extracellular $Ca^{2+}$-sensing receptors linked to phospholipase C that mobilize $Ca^{2+}$ from the ER, exposure of cells to 10 mM $Ca^{2+}$ did not decrease $[Ca^{2+}]_{Lu}$ but rather raised it. From these findings we conclude that 1) ER $Ca^{2+}$ release is strictly regulated by feedback inhibition of $[Ca^{2+}]_c$, 2) ER $Ca^{2+}$ refilling is determined by the rate of $Ca^{2+}$ influx and occurs mainly in the tiny subplasmalemmal spaces, 3) extracellular $Ca^{2+}$-induced $[Ca^{2+}]_c$ oscillations appear to be triggered not by activation of extracellular $Ca^{2+}$-sensing receptors but by the ER sensitised by elevated $[Ca^{2+}]_c$ and $[Ca^{2+}]_{Lu}$.

• International Journal of Oral Biology
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• 제34권3호
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• pp.151-155
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• 2009

The role of $Cl^-$ channels in regulatory volume decrease (RVD) in human salivary gland acinar cells was examined using a whole-cell patch clamp technique. Human tissues were obtained from healthy volunteers or from patients with oromaxillofacial tumors. During the measurements, $K^+$-free solutions were employed to eliminate contamination of whole-cell conductance by $K^+$ currents. When the cells were exposed to a 70% hypotonic solution, outward-rectifying currents, which were not observed in the resting state, were found to have significantly increased both in human labial and parotid gland acinar cells. The amplitudes of the currents were reduced in a low $Cl^-$ bath solution. Furthermore, the addition of $100{\mu}M$ 5-Nitro-2- (3-phenyl propylamino) benzoic acid (NPPB) or $100{\mu}M$ 4,4'-diisothio cyanatostilbene-2,2'-disulphonic acid (DIDS), known to partially block $Cl^-$ channels, significantly inhibited these currents. Its outward-rectifying current profile, shift in reversal potential in a low $Cl^-$ bath solution and pharmacological properties suggest that this is a $Ca^{2+}$-independent, volume activated $Cl^-$ current. We conclude therefore that volume activated $Cl^-$ channels play a putative role in RVD in human salivary gland acinar cells.

• International Journal of Oral Biology
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• 제33권4호
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• pp.125-129
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• 2008

Inositol 1,4,5-trisphosphate ($IP_3$) plays an important role in the release of $Ca^{2+}$ from intracellular stores into the cytoplasm in a variety of cell types. $IP_3$ translocation dynamics have been studied in response to many types of cell signals. However, the dynamics of cytosolic $IP_3$ in salivary acinar cells are unclear. A green fluorescent protein (GFP)-tagged pleckstrin homology domain (PHD) was constructed and introduced into a phospholipase C ${\delta}1$ (PLC ${\delta}1$) transgenic mouse, and then the salivary acinar cells were isolated. GFP-PHD was heterogeneously localized at the plasma membrane and intracellular organelles in submandibular gland and parotid gland cells. Application of trypsin, a G protein-coupled receptor activator, to the two types of cells caused an increase in GFP fluorescence in the cell cytoplasm. The observed time course of trypsin-evoked $IP_3$ movement in acinar cells was independent of cell polarity, and the fluorescent label showed an immediate increase throughout the cells. These results suggest that GFP-PHD in many tissues of transgenic mice, including non-cultured primary cells, can be used as a model for examination of $IP_3$ intracellular dynamics.

• 생명과학회지
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• 제23권11호
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• pp.1404-1408
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• 2013

본 연구에서는 마우스 췌장의 선포세포(primary acinar cell)에서 에탄올에 의해 유도된 염증성 손상에 미치는 녹차의 생리활성 물질인 EGCG의 효과를 췌장분비 효소의 활성과 선포세포에서의 기능 회복 관련 마크로 알려진 유전자들(AMPK, RKIP 및 Reg1)의 발현 조절 측면에서 조사하였다. 대조군에 비해 에탄올이 처리된 세포에서는 ${\alpha}$-amylase와 chymotrypsin의 활성이 증가되었지만, EGCG 처리에 의하여 그들 활성이 유의적으로 감소하였다. 세포 생존 및 보호와 관련 있는 AMPK 인산화 수준은 에탄올 단독 처리시 그 발현이 감소하였지만, EGCG 처리에 의하여 유의적으로 회복되었다. 세포 사멸과 세포독성 조절과 연관이 있는 RKIP 또한 그 발현 경향이 AMPK인산화 변화의 정도와 유사하였다. 또한 베타세포 재생에 관여하는 Reg1 발현은 에탄올이 처리된 선포세포에 EGCG를 처리하였을 경우 그 발현량이 유의적으로 증가하였다. 이상의 결과들을 종합하여, 항산화성 폴리페놀인 EGCG는 알코올성 췌장염으로 인한 세포의 손상이나 당뇨병 예방과 회복에 치료적 효과를 가질 수 있다고 제안한다.