• 한국동물학회지
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• 제3권1호
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• pp.1-4
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• 1960

The test group and control group, which were grouped with the same weight of five frogs (Rana nigromaculata) respectively, were adapted to light for two hours. Then the anterior lymph heart of the test group were injected with 0.5 cc of 0.1% acetyl choline and 0.5cc of Vitamin B12 (50mc gm/cc). After the frogs of this group were dark-adapted for 90 minutes, their heads were cut off under the dim red light and then their retina removed from eye-balls were extracted with 2% digitonin solution for 20 hours at $0^{\circ}C$. The results of the comparison of these two groups whose optical densities were measured before and after the illumination areas follows : (1) The group which had been injected with 0.5cc of 0.1% acetyl choline solution had the protomotive action on the regeneration of rhodopsin in comparison with the control group. (2) The group which had been injected with 25 mc gm of Vitamin B12 and 0.5cc of 0.1% acetyl choline solution had the controlling action on the regeneration of rhodopsin in comparison with the control group.

• 대한화학회지
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• 제9권1호
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• pp.33-36
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• 1965

5-Acetyl-8-hydroxyquinoline 의 酸解離定數를 $25^{\circ}C({\mu}$=0.1)에서 分光光度法과 pH滴定法에 依하여 測定하였다. 두 方法에 의하여 얻은 $pK_1$은 近似하게 一致하였으며 pK값은 8-hydroxyquinoline의 該當 pK값보다 작다. 그것은 acetyl group의 芳香族環에 대한 電子吸引性에 基因한 結果라고 생각한다.

• Clinical and Experimental Reproductive Medicine
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• 제28권4호
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• pp.295-300
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• 2001

Objective : To assess the effects of progesterone and acetyl-L-carnitine used after treated with Isolate�� gradient before semen cryopreservation-thawing on sperm parameters and membrane integrity. Material and Methods : From April 2001 to July 2001, ten normal male partner of couples who were visited in vitro fertilization (IVF) clinics. the semens were treated with $Isolate^{(R)}$ gradient before cryopreservation, spermatozoa was incubated with progesterone (1, 5 and $10{\mu}M$), acetyl-L-carnitine (2.5, 5 and $10{\mu}M$), or both (progesterone, $1{\mu}M$; and acetyl-L-carnitine, $5{\mu}M$) for 30 min. Results: There were no differences in sperm parameters and vital stain among isolate only treated group, progesterone (1, 5 and $10{\mu}M$), acetyl-L-carnitine (2.5, 5 and $10{\mu}M$) and both (progesterone, $1{\mu}M$; and acetyl-L-carnitine, $5{\mu}M$). But, in high concentration of acetyl-L-carnitine ($10{\mu}M$) treated group, sperm parameters and vital stain were decreased. The statistical method was used ANOVA (Kruskal-Wallis test) and p value was <0.01. Conclusions : Neither progesterone nor acetyl-L-carnitine show to be protective effect on the cryodamage assessed by sperm parameters and vital stain (eosin-Y stain) in normal sperm. High concentration of acetyl-L-carnitine ($10{\mu}M$), however, was harmful effect on cryoprevention.

• BMB Reports
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• 제29권4호
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• pp.277-285
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• 1996

In Pseudomonas fluorescens grown on malonate as sole carbon source, acetyl-CoA synthetase was induced, suggesting that malonate is metabolized through acetate and then acetyl-CoA. Acetyl-CoA synthetase was purified 18.6-fold in 4 steps to apparent homogeneity. The native molecular mass of the enzyme estimated by a native acrylamide gel electrophoresis was 130 kDa. The enzyme was composed of two identical subunits with a molecular mass of 67 kDa. Optimum pH was 70. The acetyl-CoA synthetase showed typical Michaelis-Menten kinetics for the substrates, acetate, ATP and CoA, whose $K_m$ values were calculated to be 33.4, 74.8, and 40.7 mM respectively. Propionate. butyrate and pentanoate were also used as substrates by the enzyme, but the rate of the formation of the CoA derivatives was decreased in the order of the increase in carbon number. The enzyme was inhibited by the group-specific reagents diethylpyro-carbonate, 2,3-butanedione, pyridoxal-5'-phosphate and N-bromosuccinimide. In the presence of substrates the inactivation rate of the enzyme, by all of the group-specific reagents mentioned above decreased, indicating the presence of catalytically essential histidine, arginine, lysine and tryptophan residues at or near the active site. Preincubation of the enzyme with ATP, $Mg^{2+}$ resulted in the increase of its susceptibility to diethylpyrocarbonate, suggesting that ATP, $Mg^{2+}$ may induce a conformational change in the active site exposing the essential histidine residue to diethylpyrocarbonate. The enzyme was acetylated in the presence of acetyl-CoA, indicating that this is one of acyl-enzyme.

• 대한화학회지
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• 제24권3호
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• pp.193-200
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• 1980

N-Acetyl-L-cysteine, $C_5H_9NO_3S,$의 결정 및 분자구조를 X-선 회절법으로 연구하였다. 이 화합물의 결정은 삼사비등축정계에 속하며 공간군은 $P_1$이다. 단위세포상수는 a = 7.04(3), b = 5.14(2), c = 8.25(3)${\AA}$, ${\alpha}$ = 106(2), ${\beta}$ = 51(1), ${\gamma}$ = 124(2)$^{\circ}$이고, 단위세포는 분자 한개만을 포함하고 있다. Weissenberg 사진촬영으로 얻은 회절반점의 총수는 629개이며 direct method를 적용하여 구조해명을 한 후 full matrix least-squares method로 정밀화하였으며 최종 R값은 0.12이다. C-카르복실기 및 N-아세틸기의 원자들은 각각 평면을 이루고 있다. 카르복실기의 산소원자 O(1)은 이웃분자의 아세틸기의 산소원자 O(3)와 $O-H{\cdot}{\cdot}{\cdot}O$ 수소결합을 하며 그 길이는 2.59${\AA}$이다.

• 한국응용과학기술학회지
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• 제31권1호
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• pp.120-129
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• 2014

나이를 먹으면 피부의 구조와 생리적 기능이 계속적인 감퇴를 일으켜서 노화된다. 외적요인에 의한 노화는 장기간에 걸친 자외선의 노출로 인한 광노화와 바람, 열, 담배 등이 원인으로 내인성 노화를 촉진시키거나 그 자체도 피부노화를 유발한다. 팔미토일 올리고 펩타이드 또는 세라마이드 올리고 펩타이드는 콜라겐 생산을 자극함으로써 피부의 상층부를 재생하며 아세틸 헥사펩타이드는 피하근육과 피부를 유연하게 하여 주름을 완화하는 성분으로 보톡스 대체 항주름 성분으로 대표적인 고기능성 뷰티성분이다. 대조군과 아세틸 헥사펩타이드 함유 7%, 14%, 20% 성분을 실험 군 A, B, C로 나눠서 주름의 변화, 모공의 변화, 수분량 변화, 과각질의 변화를 분석하였다. 아세틸 헥사펩타이드 함유 성분 분석 결과 통계적으로 주름과 모공, 수분에는 대조군에 비해 영향을 준 것으로 보이며, 과각질의 제거에서는 대조군과 실험군의 값에서 비슷한 결과를 얻을 수 있어 피시험자의 자가 평가의 만족도에서 주름과 모공, 수분에는 대조군에 비해 영향을 준 것으로 보이며, 과각질의 제거에서는 대조군과 실험군의 값에서 비슷한 결과를 얻었다.

• 대한화학회지
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• 제23권1호
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• pp.46-51
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• 1979

Silver triflate와 syn-collidine 존재하에서 3,4,6-tri-O-acetyl-2-phthalimido-${\beta}$-D-glucopyranosyl bromide (2)와 1,2;3,4-di-O-isopropylidene-${\alpha}$-D-galactopyranose (3)를 반응시켜 1,2;3,4-di-O-isopropylidene-6-O-(3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-${\beta}$-D-glucopyranosyl)-${\alpha}$-D-galactopyranose (4)를 $86{\%}$의 수득률로 얻었다. 화합물 4를 hydrazine과 작용시켜 phthalimido기와 acetyl기를 동시에 제거한후, 다시 acetyl화하고 isopropylidene기와 O-acetyl기를 가수분해하면 6-O-(2-acetamido-2-deoxy-${\beta}$-D-glucopyranosyl)-D-galactopyranose (1)가 총수득율 $65.8{\%}$로 얻어졌다. 또한 화합물 4를 변형시켜 특정위치에 hydroxyl기를 가진 몇 가지 유도체도 합성하였다.

• BMB Reports
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• 제36권3호
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• pp.312-318
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• 2003

The purpose of this study was to investigate the effects of altering relative intakes of fat and carbohydrates on serum lipid profiles, hepatic acyl-CoA synthetase (ACS), carnitine palmitoyltransferase-I (CPT-I), and the acetyl-CoA carboxlyase (ACC) mRNA level in Sprague-Dawley rats. For four weeks the rats were fed either an AIN-76 diet or one of its modified diets that were supplemented with 20% beef tallow (high-fat diet, HF) and 66.3% sucrose (highsucrose diet, HS). The HS group had significantly higher serum triglyceride and total cholesterol concentrations when compared with the other groups. Serum LDL-cholesterol concentrations in the HS and HF groups were significantly higher when compared to the normal diet (ND) group. Serum HDL-cholesterol levels of the ND and HS groups were significantly higher than those of the HF group. The hepatic total lipid level of the HF group was significantly higher than those of other groups; triglyceride levels of the HS and HF groups were significantly higher than those of the ND group. Hepatic ACS mRNA levels of the HF group were significantly higher than those of the ND group. Hepatic CPT-I mRNA levels were higher in the HF group than other groups. Also, ACC mRNA levels in the liver increased in the HF group. In conclusion, changes in the composition of dietary fat and carbohydrates could affect the hepatic ACS, CPT-I, and ACC mRNA levels. These results facilitate our understanding of the coordinated regulation of the ACS, CPT-I, and ACC mRNA levels and will serve to enhance our understanding of the molecular mechanisms that underlie the regulation of fatty acid metabolism.

• 대한화학회지
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• 제23권3호
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• pp.175-179
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• 1979

Phthalimido기와 2,2,2-trichloroethyl기는 acetic acid와 같은 산성용매에서 zine dust에 의하여 각각 3-hydroxyphthalimidino기로 환원 되거나 혹은 환원분해된다. 그러나 THF-pH 4.5 buffer 혼합용매를 사용하므로써, free carboxylic acid가 존재하지 않는 경우, phthalimido는 환원시키지 않고 2,2,2-trichloroethyl기만을 선택적으로 환원분해시킬 수 있음을 발견하였다. 따라서 $2,2,2-trichloroethyl 3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-{\beta}-D-glucopyranose$ (1)를 THF-pH 4.5 butter 혼합용매에서 zinc dust와 반응시키면, 2,2,2-trichloroethyl 기만이 선택적으로 환원분해된 $3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-{\beta}-D-glucopyranose$ (5)를 좋은 수득율로 얻을 수 있었다.

• Archives of Pharmacal Research
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• 제13권4호
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• pp.306-309
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• 1990

The synthesis of 5-hyterocyclimethyl-2'-deoxyuridines (4a-f) has been accomplished by displacement reaction of 5-(bromomethyl)-3', 5'-di-O-acetyl-2'-deoxyuridine with heterocyclic compounds, followed by removal of acetyl protecting group with methanolic ammonia. The compoudns synthesized were evaluated the inhibitory effects on L1210 cell probiferation and antiviral activities against Herpes simplex virus type 1 (HSV-1) None of the compounds exhibited sufficient biological activities.