• Korean Journal of Food Science and Technology
• /
• v.33 no.1
• /
• pp.84-88
• /
• 2001

About 100 bacterial strains producing proteolytic enzymes were isolated from Korean traditional soy paste Doenjang. Among them, strain SYG3 producing the highest level of angiotensin converting enzyme (ACE) inhibitor into the culture medium was selected and identified as Bacillus pumilus according to the Bergey's mannual of systematic bacteriology. Soybean powder as a nitrogen source and glucose as a carbon source supported high level of ACE inhibitor production. The presence of 3% NaCl also enhanced the production of ACE inhibitor in the medium. The optimum initial pH of the medium and culture temperature for the production of ACE inhibitor were 7.0 and $32^{\circ}C$, respectively. The maximal level of ACE inhibitory effect was obtained after 36 hours of cultivation under the optimized conditions, which was about 98% of inhibition ratio.

• BMB Reports
• /
• v.37 no.5
• /
• pp.552-555
• /
• 2004

Several studies have demonstrated the importance of angiotensin-converting enzyme (ACE) insertion (I)/deletion (D) polymorphisms in the pathogenesis of hypertension. This study sought to determine the association between the ACE I/D polymorphism and essential hypertension in young Pakistanis. The frequency of the ACE I/D polymorphism was established by a comparative cross-sectional survey of Pakistani patients suffering from essential hypertension and ethnically matched normotensive controls. Samples were collected from tertiary care hospitals in northern Pakistan. Hypertensive individuals were defined as those with a systolic blood pressure > 140 mmHg and/or diastolic blood pressure > 90 mmHg on three separate occasions, or those currently receiving one, or more, anti-hypertensive agents. DNA samples obtained from hypertensive (n=211) and normotensive (n=108) individuals were typed by PCR. The frequency of the ACE I/I genotype was significantly higher in hypertensive patients, aged 20-40 years, than in normotensive controls of the same age group ($\chi^2$ = 4.0, P = 0.041). Whereas no overall significant differences were observed between the I/I, I/D and D/D ACE genotypes (One way ANOVA, F=0.672; P=0.413). The association between the ACE I/I genotype and essential hypertension in individuals aged $\leq$ 40 years suggests that ACE has a role in early onset essential hypertension in Pakistan.

• Natural Product Sciences
• /
• v.29 no.2
• /
• pp.104-112
• /
• 2023

COVID-19 caused a catastrophe in human health. People infected with COVID-19 also suffer from various clinical illnesses during and after the infection. The Boerhavia diffusa plant is well known for its antihypertensive activity. ACE-II inhibitors and calcium channel blockers are reported as mechanisms for the antihypertensive activity of B. diffusa phytoconstituents. Various studies have said ACE-II is the virus's binding site to attack host cells. COVID-19 treatment commonly employs a variety of synthetic antiviral and steroidal drugs. As a result, other clinical illnesses, such as hypertension and hyperglycemia, emerge as serious complications. Safe and effective drug delivery is a prime objective of the drug development process. COVID-19 is treated with various herbal treatments; however, they are not widely used due to their low potency. Many herbal plants and formulations are used to treat COVID-19 infection, in which B. diffusa is the most widely used plant. The current study relies on discovering active phytoconstituents with ACE-II inhibitory activity in the B. diffusa plant. As a result, it can be used as a treatment option for patients with COVID-19 and related diseases. Different phytoconstituents of the B. diffusa plant were selected from the reported literature. The activity of phytoconstituents against ACE-II proteins has been studied. Molecular docking and ligand-protein interaction computation tools are used in the in-silico experiment. Physicochemical, drug-likeness, water solubility, lipophilicity, and pharmacokinetic parameters are used to evaluate phytoconstituents. Liriodenine has the best drug-likeness, bioactivity, and binding score characteristics among the selected ligands. The in-silico study aims to find the therapeutic potential of B. diffusa phytoconstituents against ACE-II. Targeting ACE-II also shows an effect against SARS-CoV-2. It can serve as a rationale for designing a drug for patient infected with COVID-19 and associated diseases.

• Journal of Genetic Medicine
• /
• v.3 no.1
• /
• pp.11-13
• /
• 1999

Recently it was reported that Insertion/Deletion polymorphism in the gene coding for Angiotensin-Converting Enzyme (ACE) is associated with human capacity for physical performance. This study was performed to genotyping of the ACE gene to determine the correlation between elite endurance performance and ACE I/D gene polymorphism. DNA sample was obtained from peripheral blood, hair roots and mouth epithelial cell in 739 general population and 200 elite athletic performance students. The ACE gene was amplified by polymerase chain reaction (PCR) using allele specific oligonucleotide primers. 155, 525 bp and 237 bp PCR products indicating the presence of insertion(I) and deletion(D) alleles, respectively, were clearly resolved after electrophoresis on a 2% agarose gel with ethidium bromide. Of the 200 elite athletic performance population subjects, 68(34%) showed ACE genotype 11,100(50%) genotype ID and 32(16%) genotype DD. Of the 739 general population subjects, 259(35.1%) showed ACE genotype 11,363(49.1%) genotype ID and 117(15.8%) genotype DD. Therefore ACE I/D gene polymorphism was not associated with human capacity for physical performance.(p>0.05)

• Proceedings of the Korean Society For Composite Materials Conference
• /
• 2004.10a
• /
• pp.6-8
• /
• 2004

A big boom in nanocomposites research has landed also in Japan. As a virtual 'center of excellence' in composites technology there, ACE TeC of ISTA/JAXA has led pioneering portions of nanocomposites research particularly in mechanical properties oriented applications. An overview of research activities based on nano-technologies in ACE TeC/JAXA will be given first and some remarkable results will be introduced briefly.

• Proceedings of the KIEE Conference
• /
• 2002.11c
• /
• pp.465-468
• /
• 2002

Today large corporations are constructing distributed server environment. Many corporations are respectively operating Web server, FTP server, Mail server and DB server on heterogeneous operation. However, there is the problem that a manager must manage each server individually. In this paper, we present distributed FTP server for log mining system on ACE. Proposed log mining system is based upon ACE (Adaptive Communication Environment) framework and data mining techniques. This system provides a united operation with distributed FTP server.

• Tuberculosis and Respiratory Diseases
• /
• v.58 no.1
• /
• pp.25-30
• /
• 2005

Background : An insertion-deletion polymorphism of angiotensin converting enzyme (ACE) gene has been shown to be associated with enzyme activity levels of ACE. Reported results that have been mutually contradictory about asthmatic hypersensitiveness and occurrence according to ACE gene insertion (I)/deletion (D) polymorphism. Also, the involvement of the ACE genes as the genetic basis of bronchial asthma is currently controversy. We investigated whether there was any association between polymorphisms of the ACE genes and airway hyper-responsiveness in chronic obstructive pulmonary disease (COPD). Methods : A total of 100 patients with COPD were enrolled in this study. The ACE genotypes were determined in all subjects by polymerase chain reaction. Pulmonary function test including bronchodilator response (BDR), methacholine bronchial provocation test (MBPT) were done in those patients. Airway hyper-responsiveness include any findings of positive BDR or MBPT. Results : In COPD patients, the ACE genotype distribution did not differ significantly among groups of patients with severities of COPD, and with or without airway hyper-responsiveness. Conclusions : These results suggest that polymorphisms of the ACE gene may not be associated with airway hyper-responsiveness, development and severity of COPD.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.44 no.2
• /
• pp.118-125
• /
• 2011

In this study, an angiotensin I-converting enzyme (ACE) inhibitor from squid skin was purified and characterized. Squid (Todarodes pacificus) skin protein isolates were hydrolyzed using six commercial proteases: alcalase, ${\alpha}$-chymotrypsin, neutrase, papain, pepsin, and trypsin. The peptic hydrolysate had the highest ACE inhibitory activity. The ACE inhibitory peptide was purified using Sephadex G-25 column chromatography and reverse phase high-performance liquid chromatography (HPLC) with a $C_{18}$ column. The purified ACE inhibitory peptide was identified and sequenced, and found to consist of seven amino acid residues: Ser-Ala-Gly-Ser-Leu-Val-Pro (657Da). The $IC_{50}$ value of the purified ACE inhibitory peptide was 766.2 ${\mu}M$, and Lineweaver-Burk plots suggested that the purified peptide acts as a noncompetitive ACE inhibitor. These results suggest that the ACE inhibitory peptide purified from the peptic hydrolysate of squid skin may be of benefit in developing antihypertensive drugs and functional foods.

• Proceedings of the Korean Information Science Society Conference
• /
• 2001.10c
• /
• pp.304-306
• /
• 2001

ACE2000 MPLS 시스템은 대용량 국가 교환시스템으로 사용하기 위해 개발한 ACE2000 ATM 교환시스템에 Ships-in-the-night 모드 형태로 MPLS 기능을 추가함으로써 사용자들에게 차별화 된 서비스를 제공할 수 있는 시스템이다. MPLS 시스템에서는 채널 사용의 유연성과 채널 자원의 효율적인 사용을 위해 VC 머징 기능을 요구하고 있다. ACE2000 MPLS 시스템에서는 고성능 SAR 소자를 이용하여 VC 머징 모듈을 개발하여 사용하고 있다. VC 머징 기능을 위해서는 고성능 SAR로 구성되어 있는 입력부로부터 패킷을 받아들여 채널 연결설정 정보에 따른 VC 머징 제어기의 패킷 처리 후 또 하나의 고성능 SAR로 구성되어 있는 출력부로 전달하여야 만다. VC 머징 제어기에서는 머징 수신부로부터 UTOPIA-2 인터페이스를 통해 패킷을 받아들여 일반 AAL-5 데이터 패킷, 포워딩 엔진 제어 및 PPP 제어 등틀 위한 EFC 패킷 그리고, 순수 ATA 셀로 구분/저리한 후 UTOPIA-2 인터페이스를 통해 머징 송신부로 보내고 있다. 본 논문에서는 ACE2000 MPLS 시스템에 도입된 VC 머징 기술에 대한 내용으로서 고성능 SAR 소자를 이용한 VC Merger의 핵심 기술인 VC머징 제어기 설계기술에 대하여 언급하였다.

• Korean Journal of Mathematics
• /
• v.26 no.4
• /
• pp.757-775
• /
• 2018

For any non-negative real number ${\epsilon}_0$, we shall introduce a concept of the ${\epsilon}_0$-dense subset of $R^m$. Applying this concept, for any sequence {${\epsilon}_n$} of positive real numbers, we also introduce the concept of the {${\epsilon}_n$}-attainable sequence and of the points of {${\epsilon}_n$}-attainable ace in the open subset of $R^m$. We also study the characteristics of those sequences and of the points of {${\epsilon}_n$}-dense ace. And we research the conditions that an {${\epsilon}_n$}-attainable sequence has no {${\epsilon}_n$}-attainable ace. We hope to reconsider the social consideration on the ace in social life by referring to these concepts about the aces.