• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.1
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• pp.43-48
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• 2003

The effect of rare earth(RE) at three difference levels of nitrogen on the growth and nitrate content of chicory (Cichorium intybus L.) were studied in terms of leaf size, fresh and dry weight increase, and chlorophyll and nitrate content grown in plastic pots within glasshouse. Leaf size was enlarged as the level of nitrogen applied was high, and leaf within, length, and thickness of leaf were increased by RE treatment showing significant difference at high nitrogen(N+1) plot. As the level of nitrogen applied was high, as fresh and dry weight per plant was increased significantly(p<0.05), and sprinkling cabbage with RE increased fresh and dry matter yield to 2∼12, 4∼6.2% more, respectively. Dry matter content of Chinese cabbage was increased by RE sprinkling. At all levels of nitrogen fertilized chlorophyll contents were increased by RE treatment. As the level of nitrogen was high, as the content of chlorophyll was highly increased by RE. Nitrate was accumulated more at high level of nitrogen application, but nitrate was decreased by RE application, 40% decrease at very high nitrogen plot(N+2). RE stimulated the growth of chicory with high chlorophyll content, and showed the possibility producing high quality agricultural products low in nitrate content.

• Journal of Forest and Environmental Science
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• v.34 no.1
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• pp.12-23
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• 2018

Quantitative information on biomass and available nutrients are essential for developing sustainable forest management strategies to regulate atmospheric carbon. An attempt was made at Chilapatta Reserve Forest in Duars region of West Bengal to quantify its above and below ground carbon along with available "N", "P" and "K" in the soil. Stratified random nested quadrats were marked for soil, biomass and litter sampling. Indirect or non-destructive procedures were employed for biomass estimation. The amount of these available nutrients and organic carbon quantified in soil indicates that the forest soil is high in organic carbon and available "K" and medium in phosphorus and nitrogen. The biomass, soil carbon and total carbon (soil C＋C in plant biomass) in the forest was 1,995.98, 75.83 and $973.65Mg\;ha^{-1}$. More than 90% of the carbon accumulated in the forest was contributed by the trees. The annual litter production of the forest was $5.37Mg\;ha^{-1}$. Carbon accumulation is intricately linked with site quality factors. The estimated biomass of $1,995.98Mg{\cdot}ha^{-1}$ clearly indicates this. The site quality factor i.e. tropical moist deciduous with optimum availability of soil nutrients, heavy precipitation, high mean monthly relative humidity and optimum temperature range supported luxuriant growth which was realized as higher biomass accumulation and hence higher carbon accumulated.

• Journal of Bio-Environment Control
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• v.21 no.4
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• pp.327-335
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• 2012

Nutrient uptake of each part of grapevine in the new grapevine cultivars (Jinok, Hongisul) was analyzed for making standard of annual fertilizations at four years. One year grown diploid cultivar 'Jinok' was showed more vigorous growth of root than other cultivar. Annually total nutrient of grapevine was absorbed with the same ratio of three major nutrients (Nitrogen, Phosphate, and Potassium). However about 30% of total absorbed nutrient of 3~4 year grown grapevine was distributed bunches. Potassium was measured as major nutrient in the bunch, accumulated in the peel and flesh (about 1% of D.W.). Magnesium was mainly accumulated in the petiole (about 1% of D.W.). Calcium has accumulated in the leaf (about 0.95% of D.W.), that concentration in this part was similar concentration of nitrogen (about 1.25% of D.W.). Nutrient requirement of four year grown 'Jinok' (N; 55.5 g, P; 7.7 g, K; 42.0 g, Ca; 34.6 g, Mg; 11.1 g) required less fertilizers than 'Campbell Early' (N; 57.4 g, P; 7.9 g, K; 44.4 g, Ca; 37.3 g, Mg; 12.2 g) needed. 'Hongisul' required less fertilizers compared to other grapevine cultivars, but cultural practice system for production of grape should be develop to improve their poor bud burst and fruit set.

• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1408-1415
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• 2008

To produce polyhydroxyalkanoate (PHA) from inexpensive substrates by bacteria, vegetable-oil-degrading bacteria were isolated from a rice field using enrichment cultivation. The isolated Pseudomonas sp. strain DR2 showed clear orange or red spots of accumulated PHA granules when grown on phosphate and nitrogen limited medium containing vegetable oil as the sole carbon source and stained with Nile blue A. Up to 37.34% (w/w) of intracellular PHA was produced from corn oil, which consisted of three major 3-hydroxyalkanoates; octanoic (C8:0, 37.75% of the total 3-hydroxyalkanoate content of PHA), decanoic (C10:0, 36.74%), and dodecanoic (C12:0, 11.36%). Pseudomonas sp. strain DR2 accumulated up to 23.52% (w/w) of $PHA_{MCL}$ from waste vegetable oil. The proportion of 3-hydroxyalkanoate of the waste vegetable-oil-derived PHA [hexanoic (5.86%), octanoic (45.67%), decanoic (34.88%), tetradecanoic (8.35%), and hexadecanoic (5.24%)] showed a composition ratio different from that of the corn-oil-derived PHA. Strain DR2 used three major fatty acids in the same ratio, and linoleic acid was the major source of PHA production. Interestingly, the production of PHA in Pseudomonas sp. strain DR2 could not occur in either acetate- or butyrate-amended media. Pseudomonas sp. strain DR2 accumulated a greater amount of PHA than other well-studied strains (Chromobacterium violaceum and Ralstonia eutropha H16) when grown on vegetable oil. The data showed that Pseudomonas sp. strain DR2 was capable of producing PHA from waste vegetable oil.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.3
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• pp.265-270
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• 2016

Accumulated Phosphate can be released by ligand exchange reaction of organic acids. The objective of this study was to evaluate effects of the organic acids on the availability of phosphate and the growth of crop in phosphate and salts accumulated soil. Soil samples were collected from farmer's plastic film house. Available phosphate and electrical conductivity of soil were $3,005mg\;kg^{-1}$ and $16.63mg\;kg^{-1}$ which were 6 and 8 times higher than the optimum range of soil for crop growth, respectively. Corns were cultivated in pots for 2 months. Treatments were no treatment (control), phosphate fertilizer (P), citric acid (CA) 1, 5, 10 mM, and oxalic acid (OA) 1, 5, 10 mM. Water soluble phosphorus, available phosphate, corn growth and uptake were determined after cultivation. Results showed that organic acids increased water soluble phosphorus and available phosphate. For the level of 10 mM, the order of effectiveness of organic acids for water soluble P was citric acid (44%) > oxalic acid (32%). Height and dry weight of corns were increased significantly by the treatment of citric acid 1 and 5 mM. Also, corn absorbed more phosphorus, nitrogen, potassium, calcium and magnesium in the treatment of citric acid 1 mM than these of other treatments. Even though phosphate availability of soil was enhanced by addition of citric acid 10 mM, the growth of corns decreased because high concentration of citric acid caused salt damage by increasement of electrical conductivity. Thus, the citric acid of 1 mM has the potential to improve the availability of phosphate and the healthy growth of corns.

• Mycobiology
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• v.33 no.1
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• pp.51-60
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• 2005

Pot greenhouse experiments were carried out to attempt to increase the salinity tolerance of one of the most popular legume of the world; cowpea; by using dual inoculation of an Am fungus Glomus clarum and a nitrogen-fixer Azospirillum brasilense. The effect of these beneficial microbes, as single- or dual inoculation-treatments, was assessed in sterilized loamy sand soil at five NaCl levels ($0.0{\sim}7.\;2ds/m$) in irrigating water. The results of this study revealed that percentage of mycorrhizal infection, plant height, dry weight, nodule number, protein content, nitrogenase and phosphatase activities, as well as nutrient elements N, P, K, Ca, Mg were significantly decreased by increasing salinity level in non-mycorrhized plants in absence of NFB. Plants inoculated with NFB showed higher nodule numbers, protein content, nitrogen concentration and nitrogenase activities than those of non-inoculated at all salinity levels. Mycorrhized plants exhibited better improvement in all measurements than that of non-mycorrhized ones at all salinity levels, especially, in the presence of NFB. The concentration of $Na^+$ was significantly accumulated in cowpea plants by rising salinity except in shoots of mycorrhizal plants which had $K^+/Na^+$ ratios higher than other treatments. This study indicated that dual inoculation with Am fungi and N-fixer Azospirillum can support both needs for N and P, excess of NaCl and will be useful in terms of soil recovery in saline area.

• Nutritional Sciences
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• v.6 no.4
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• pp.189-194
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• 2003

Most of the ethyl alcohol consumed by humans is oxidized to acetaldehyde in the liver by the cytoplasmic alcohol dehydrogenase (ADH) system. For this ADH-catalyzed oxidation of alcohol, $NAD^+$ is required as the coenzyme and $NAD^+$becomes reduced to NADH. As the $NAD^+$becomes depleted and NADH accumulates, alcohol oxidation is reduced. For continued alcohol oxidation, the accumulated NADH must be quickly reoxidized to $NAD^+$, and it is this reoxidation of NADH to $NAD^+$that is known to be the rate-limiting step in the overall oxidation rate of alcohol The reoxidation of NADH to $NAD^+$is catalyzed by lactate dehydrogenase in the cytoplasm of hepatocytes, with pyruvate being utilized as the substrate. The pyruvate may be supplied from alanine as a result of amino acid metabolism via the urea cycle. Also, glutamine is thought to help with the supply of pyruvate indirectly, and to activate the urea cycle by producing $NH_3$. Thus, in the present study, we have examined the effects of alanine and glutamine on the alcohol oxidation rate. We utilized isolated perfused liver tissue in a system where media containing alanine and glutamine was circulated. Our results showed that when alanine (5.0mM) was added to the glucose-free infusion media, the alcohol oxidation rate was increased by 130%. Furthermore, when both glutamine and alanine were added together to the infusion media, the alcohol oxidation rate increased by as much as 190%, and the rate of urea nitrogen production increased by up to 200%. The addition of glutamine (5.0mM) alone to the infusion media did not accelerate the alcohol oxidation rate. The increases in the rates of alcohol oxidation and urea nitrogen production through the addition of alanine and glutamine indicate that these amino acids have contributed to the enhanced supply of pyruvate through the urea cycle. Based on these results, it is concluded that the dietary supplementation of alanine and glutamine could contribute to increased alcohol detoxification through the urea cycle, by enhancing the supply of pyruvate and $NAD^+$to ensure accelerated rates of alcohol oxidation.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.314-320
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• 2014

BACKGROUND: Run off from agricultural sites contaminates water bodies with nitrogen which is toxic and causes eutrophication when excessively accumulated. Hence, the interest in monitoring nitrogen toxicity in aquatic environment has been continuously increasing. METHODS AND RESULTS: To detect a real time toxicity of various nitrogen compounds, we applied biomonitoring method (biosensor) based on sulfur-oxidizing bacteria (SOB). The toxicity biomonitoring test was conducted in semi-continuous mode in a reactor filled with sulfur particles (2~4 mm diameter) under aerobic condition. Relative toxicity was simply determined by measuring the change in electrical conductivity (EC). Various nitrogenous compounds at different concentrations were evaluated as a potential toxic substance. Nitrite was found to be very toxic to SOB with a 90% inhibition even when the concentration as low as 3 mg/L. However, nitrate and ammonia have any inhibitory effect on SOB's activity. CONCLUSION: The biosensor based on SOB responded sensitively to nitrite even at substantially low concentrations. Therefore, it can be used as a reliable biological alarm system for rapid detection of contaminants due to its simplicity and sensitive nature.

• Microbiology and Biotechnology Letters
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• v.12 no.4
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• pp.271-276
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• 1984

Effect of C/N ratios on the cell growth, cell lipid, cell protein and fatty acids of Rhodotorula sp. was examined. At low concentration of nitrogen in cultural medium the growth of the yeast was retarded, whereas its growth was favorable at high concentration but lipid yield was decreased due to a high content of cell protein. In order to increase lipid yield, the optimum C/N ratio obtained was 45-50 (5% glucose, 0.0414% nitrogen). During the cultivation cell protein synthesis occurred mainly in initia stage and the lipid was accumulated rapidly at the late phase. The highest content of total fatty acid was observed at the optimum C/N ratio. The higher C/N ratio was the more unsaturation degree of fatty acid decreased. It was attributed principally to a decreased oleic acid with palmitic acid increased. No significant changes occurred in content of other fatty acids.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.486-491
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• 2002

Spikelet number and its components of rice plant are closely associated with nitrogen accumulation and biomass production during panicle formation stage. To elucidate this relationship and also compare the differences of the sink formation efficiency among cultivars, spikelet number, its components, nitrogen content, nonstructural carbohydrate content, and plant dry matter were investigated under 5 nitrogen levels with two split application methods and shading treatments by using three rice varieties. The nitrogen amount in shoot at panicle initiation stage and at 15 days after panicle initiation showed significant positive correlation with primary rachis branches per square meter, and that at 15 days after panicle initiation and at heading stage with secondary rachis branches per square meter, Primary and secondary rachis branches per square meter showed positive significant correlation with the shoot dry weight at panicle initiation stage and at 15 days after panicle initiation stage, respectively, The amount of degenerated secondary rachis branches and spikelets per square meter showed significant negative correlation with the dry weight and nonstructural carbohydrate increase of stem during 15days after panicle initiation, and the contents of nonstructural carbohydrate at 15 days after panicle initiation. Spikelets per unit area showed significant positive correlation with nitrogen amount in shoot and shoot dry weight at heading stage. The sink formation efficiency expressed as the spikelet number produced by the unit amount of nitrogen in shoot at heading stage was higher in Nampoongbyeo than Choocheongbyeo and Hwaseongbyeo. Sink formation efficiency was negatively correlated with the dry weight increase of shoot and stem during reproductive stage. but not significantly with that of leaf in all varieties. Sink formation efficiency was not significantly correlated with nonstructural carbohydrate, but was significantly negatively correlated with structural carbohydrate increase during reproductive stage.