Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Toxicity Response of Biosensor Using Sulfur-Oxidizing Bacteria to Various Nitrogenous Compounds

다양한 질소화합물에 대한 황산화미생물 바이오센서의 응답 특성

  • Hwang, Ji-Hoon (Department of Biological Environment, Kangwon national university) ;
  • Kang, Woo-Chang (Department of Biological Environment, Kangwon national university) ;
  • Shin, Beom-Soo (Department of Biosystems Engineering, Kangwon national university) ;
  • Chae, Kyu-Jung (Department of Environmental Engineering, Korea maritime and ocean university) ;
  • Oh, Sang-Eun (Department of Biological Environment, Kangwon national university)
  • 황지훈 (강원대학교 바이오자원환경학과) ;
  • 강우창 (강원대학교 바이오자원환경학과) ;
  • 신범수 (강원대학교 바이오시스템공학과) ;
  • 채규정 (한국해양대학교 환경공학과) ;
  • 오상은 (강원대학교 바이오자원환경학과)
  • Received : 2014.10.29
  • Accepted : 2014.12.08
  • Published : 2014.12.31
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Abstract

BACKGROUND: Run off from agricultural sites contaminates water bodies with nitrogen which is toxic and causes eutrophication when excessively accumulated. Hence, the interest in monitoring nitrogen toxicity in aquatic environment has been continuously increasing. METHODS AND RESULTS: To detect a real time toxicity of various nitrogen compounds, we applied biomonitoring method (biosensor) based on sulfur-oxidizing bacteria (SOB). The toxicity biomonitoring test was conducted in semi-continuous mode in a reactor filled with sulfur particles (2~4 mm diameter) under aerobic condition. Relative toxicity was simply determined by measuring the change in electrical conductivity (EC). Various nitrogenous compounds at different concentrations were evaluated as a potential toxic substance. Nitrite was found to be very toxic to SOB with a 90% inhibition even when the concentration as low as 3 mg/L. However, nitrate and ammonia have any inhibitory effect on SOB's activity. CONCLUSION: The biosensor based on SOB responded sensitively to nitrite even at substantially low concentrations. Therefore, it can be used as a reliable biological alarm system for rapid detection of contaminants due to its simplicity and sensitive nature.

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References

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