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Seasonal Variations of Metacercarial DEnsity of Clonorchis sinensis in Fish Intermediate Host, Pseudorasbora parva (간흡충 유행지역에서 참붕어내 피낭유충 감염밀도의 계절적 변동)

  • Gang, Sin-Yeong;Kim, Seok-Il;Jo, Seung-Yeol
    • Parasites, Hosts and Diseases
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    • v.23 no.1
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    • pp.87-94
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    • 1985
  • The seasonal variations of the rate and intensity of metacercarial infection of C. sinensis in P. parva were observed. The fish were collected at Sun-Am River which located in Kim-Hae City, Kyong-Sang Nam Do (=Province), Korea, from March 1983, to February 1984 every month. A total of 788 fish was examined. The number of metacercariae in each fish was individually counted after the individual digestion by artificial gastric juice. The results were as follows: 1. During one year, 513 (65.1%) out of 788 fish were infected with metacercariae. In May, June, July and September, the infection rates ranged from 82.0% to 98.6% whereas the rates was relatively low in March, April, November and February ranging from 11.4% to 64.7%. 2. The intensity of infection was similar with those of infection rates. The mean intensity per infected fish was 103.0 and standard deviation was 118.9 throughout one year. The highest mean intensity was in June (294.8) and the lowest in November (11.1). 3. The observed frequency of fish with certain intensities of metacercariae were fitted to theoretical equations derived from negative binomial distribution in March, April, November and February (p>0.05). Meanwhile, the equation of lognormal distribution were fitted with the observed frequencies in May, June, July and September (p>0.05, p>O. 75). The variance/mean ratio varied by month. The value was the highest in July (814.3) and the lowest in November (158.8). Unlike our hypothesis, the metacercarial density of Clonorchis sinensis in its the most favorable fish host, Pseudorasbora parva showed considerable seasonal variations in the hyperendemic area. The possible factors were discussed.

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Characteristics of Low Density Fiberboards Bonded with Different Adhesives for Thermal Insulation (II) - Formaldehyde·Total Volatile Organic Compounds Emission Properties and Combustion Shapes - (다양한 접착제로 제조한 단열재용 저밀도섬유판의 특성(II) - 폼알데하이드·총휘발성유기화합물 방출 특성 및 연소 형상 -)

  • Jang, Jae-Hyuk;Lee, Min;Kang, Eun-Chang;Lee, Sang-Min
    • Journal of the Korean Wood Science and Technology
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    • v.45 no.5
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    • pp.580-587
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    • 2017
  • Woodfiber insulation board can be considered as a one of the key material for low energy consumption, comfortable and safety construction of residential space because of its eco-friendly and high thermal insulation performance. This study was carried out to investigate the formaldehyde (HCHO) total volatile organic compounds (TVOC) emission properties and combustion shapes by flame test of low density fiberboards (LDFs) prepared with different adhesives. HCHO TVOC emission and combustion properties of LDFs prepared by melamine urea formaldehyde (MUF), phenol formaldehyde (PF), emulsified methylene diphenyl diisocyanate (eMDI) and latex resin adhesives were measured by desiccator method, 20 L chamber method, and flame test, respectively. As results, LDFs manufactured by MUF, eMDI and latex resin adhesives satisfied the Super $E_0$ grade of HCHO emission performance except PF resin. Furthermore, TVOC emission of all LDFs were satisfied the Korean indoor air quality standard (below $400{\mu}g/m^2{\cdot}h$). Especially, LDF with eMDI resin adhesive showed the lowest HCHO and TVOC emissivity, that $0.14mg/{\ell}$, $12{\mu}g/m^2{\cdot}h$, respectively. However, eMDI emitted the small amount ($3{\mu}g/m^2{\cdot}h$) of toluene in VOC components. In the flame test, LDF with MUF resin adhesives showed the most favorable shape after flame test compare to LDFs prepared other adhesives. Based on HCHO and TVOC emission, and combustion shapes, MUF resin adhesive may be recommended to prepare LDF for insulation purpose.

The Long-term Clinical Outcomes after Coronary Artery Bypass Graft Surgery (관상동맥 우회술의 장기 임상성적)

  • Park, Chan Beom;Jo, Min-Seop;Kim, Young-Du;Jin, Ung;Moon, Seok-Whan;Kim, Chi-Kyung;Jo, Keon Hyon
    • Journal of Chest Surgery
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    • v.42 no.1
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    • pp.22-27
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    • 2009
  • Background: Coronary artery bypass grafting (CABG) is the standard surgical treatment for coronary artery disease. Although there are many clinical reviews of the long term results after CABG in the Western countries, not many such studies have been done for Korea. Therefore, we reviewed the long term clinical results for the patients who underwent CABG at our hospital. Material and Method: We retrospectively reviewed the medical records of 342 patients who underwent CABG at our hospital from February 1984 to December 2006, which is when CABG was first performed in our institution. A total of 286 patients (83.6%) were able to be followed-up, and the mean follow-up period was $75.7{\pm}46.1$ months. Result: The early mortality rate was 5.6%, and late mortality rate was 23.1%. The one-year survival rate, the five-year survival rate, the ten-year survival rate and the fifteen-year survival rate were 91.5%, 82.1%, 60.7% and 50.0%, respectively. The survival rate was significantly lower for the patients over the age of 60 (p=0.002) and for those with diabetes mellitus (p=0.000), hypertension (p=0.002), multivessel disease (p=0.006) and left ventricular dysfunction (p=0.015). No significant difference was observed between the genders. Multivariate analysis showed that the statistically significant risk factors were diabetes mellitus (p=0.001), age (p=0.005) and those cases for which the left internal thoracic artery was not used (p=0.037). Conclusion: CABG is the effective method of treatment for coronary artery disease. Therefore, active usage of the internal thoracic artery and appropriate medical treatment after surgery, and especially for diabetes mellitus patients, are mandatory for achieving good long-term survival.

Determination of finasteride in human serum by LC-MS/MS (LC-MS/MS를 이용한 혈청 중 finasteride 분석)

  • Nam, Hye-Seon;Nam, Kyong-Hee;Jung, Su-Hee;Lee, Jang-Woo;Kang, Jin-Yeong;Hong, Soon-Keun;Kim, Tae-Sung;Kang, Tae-Seok;Yoon, Hae-Jung;Lee, Kwang-Ho;Rhee, Gyu-Seek
    • Analytical Science and Technology
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    • v.24 no.5
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    • pp.345-351
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    • 2011
  • A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS) method was developed and validated for the determination of finasteride in human serum. Beclomethasone was used as internal standard (IS) and liquid-liquid extraction (LLE) using methyl tert-butyl ether (MTBE) was carried out to isolate analyte. The mass transitions monitored in multiple reaction monitoring (MRM) in positive ion mode were m/z 373.2${\rightarrow}$305.2 for finasteride and m/z 409.3${\rightarrow}$391.2 for IS. Retention times of finasteride and IS were 5.81 and 5.46 min, respectively. The limit of quantitation (LOQ) was 0.1 ng/mL and the calibration curve showed good linearity in the range of 0.1~20.0 ng/mL ($R^2$=0.9997). The intra-day assay precision and accuracy were in the range 6.3~10.6% and 97.3~103.6%, respectively, and the inter-day assay precision and accuracy were in the range 0.8~5.2% and 99.8~102.5%, respectively. The sample extract recovery of the method was 80~83%.

Determination of Net Energy and Protein Requirements for Growth in Hanwoo Steers by Comparative Slaughter Experiment (비교도체법에 의한 한우 거세우의 증체에너지 및 단백질 요구량)

  • Kim, K.H.;Oh, Y.G.;Lee, S.C.;Shin, K.J.;Chung, W.T.;Kang, S.W.;Hong, S.K.;Ju, J.C.;Baek, B.H.
    • Journal of Animal Science and Technology
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    • v.49 no.1
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    • pp.41-50
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    • 2007
  • Data from a comparative slaughter experiment with two hundreds of Korean native (Hanwoo) beef steers were utilized to determine net energy and protein requirements for growth (NEg and NPg). Eight randomly selected steers were used in the initial slaughter group of 6 month of age and the remaining steers allocated to treatments within two groups. The restricted groups were fed 1.2 to 1.5% concentrate per kg body weight (BW) in the growing period (6~12 month of age), 1.7 to 1.8% concentrate per kg BW in the early fattening period (13~18 month of age), and concentrate feed ad libitum in the late fattening period (19~30 month of age). Ad libitum groups were fed concentrate ad libitum through the whole period. Rice straw was offered ad libitum for 24 month of two feeding systems. The steers were slaughtered when animals reached every 2 month from 8 to 30 month of age. For all body composition determinations, whole empty body components were weighed, taken each proportional subsample was ground for chemical analysis. Equations developed with the pooled data to predicted NEg and NPg were similar to the equations of Japanese Feeding Standard for Beef Cattle (2000). First equations were developed to predicted NEg; NEg = 0.05332×LBW0.75×DG for restricted treatment and NEg = 0.04912×LBW0.75×DG for ad libitum treatment. Second equations were developed to predicted NPg; NPg = DG × (224.7-0.251×LBW) for restricted treatment and NPg=DG×(210.1-0.214×LBW) for ad libitum treatment.

Emission Rate of Greenhouse Gases from Bedding Materials of Cowshed Floor: Lab-scale simulation study (우사깔짚에서 발생되는 온실가스 배출량 산정: 모의 실험결과)

  • Cho, Won Sil;Lee, Jin Eui;Park, Kyu Hyun;Kim, Jeong Dae;Ra, Chang Six
    • Journal of Animal Science and Technology
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    • v.55 no.1
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    • pp.67-74
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    • 2013
  • To know the emission amount of greenhouse gases from bedding materials of cowshed floor, the emission rates of methane ($CH_4$) and nitrous oxide ($N_2O$) gases from a simulated cowshed floor (SCF) with sawdust that manure loading rate into the bedding material could be accurately controlled were assessed in this study. The manure loading rates of Korean beef and Holstein dairy cattle into the SCF of $0.258m^2$ surface area with 10 to 15 cm height sawdust were $1.586kg/m^2/d$ and $3.588kg/m^2/d$, respectively, and those were calculated on the basis of "Standard model for sustainable livestock" and "Data for excretion amount of manure from livestock". All experiments were done in triplicates in three different seasons (May to July, Sep. to Nov., and Feb. to Apr.) using 12 SCFs. The effects of bedding material thickness on $CH_4$ and $N_2O$ emission from SCFs for both Korean beef cattle and Holstein dairy cattle were not statistically significant (p<0.05). Emission amount of $CH_4$ and $N_2O$ per square meter of SCF for Holstein dairy cattle was 7.5 and 1.2 times higher than that of Korean beef cattle, respectively. The yearly $CH_4$ amount per head was 17.7 times higher in Holstein dairy cattle, obtaining 130.4 g/head/year from SCF for Holstein dairy cattle and 7.4 g/head/year from SCF for Korean beef cattle, and $N_2O$ was also 3.8 times higher in Holstein dairy cattle (3,267 g/head/year in Korean beef cattle and 14,719 g/head/year in Holstein dairy cattle). However, the $N_2O$-N per loaded nitrogen into SCF was higher in Korean beef cattle, having 0.2148 and 0.1632 kg $N_2O$-N/kg N in Korean beef cattle and Holstein dairy cattle, respectively, and those values were 3.07 and 2.33 times higher than that of Intergovernmental Panel on Climate Change (IPCC) 2006 guideline (GL) (0.07 kg $N_2O$-N/kg N).

Residual characteristic of tebuconazole and fludioxonil in Allium victorialis (Allium ochotense Prokh.) (소면적 재배작물 산마늘(Allium ochotense Prokh.) 중 살균제 Tebuconazole 및 Fludioxonil의 잔류특성)

  • Woo, Min-Ji;Hur, Kyung-Jin;Kim, Ji-Yoon;Saravanan, Manoharan;Kim, Se-Weon;Hur, Jang-Hyeon
    • The Korean Journal of Pesticide Science
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    • v.19 no.4
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    • pp.354-360
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    • 2015
  • In recent years, Allium victorialis has been extensively used as a pharmacological agent for various diseases in the form of anti-arteriosclerotic, anti-diabetic and anti-cancer. Allium victorialis is severely affected by various fungal diseases since it naturally grow in the shady and humid environments in Korea. In this case, different types of fungicides are applied to control the fungal diseases in Allium victorialis. The present study was aimed to determine the residual characteristics of two fungicides namely tebuconazole and fludioxonil on Allium victorialis. For this study, the fungicides were drenched soil on Allium victorialis in the cultivation area Pyeongchang by the standard (two thousand fold) and double (thousand fold) dilutions. At the end of $15^{th}$, $30^{th}$ and $40^{th}$ days samples were collected for residue analysis. Residues of tebuconazole and fludioxonil were analyzed using GC/NPD (Gas Chromatography/Nitrogen Phosphorus Detector) and their recovery were found to be 108.8~119.5% and 91.3~104.8%, respectively. The method of limits of quantification for both fungicides was $0.01mg\;kg^{-1}$. Further, the results of this study shows that the residue levels of both fungicides on Allium victorialis were <$0.01{\sim}0.12mg\;kg^{-1}$ and $0.01{\sim}0.09mg\;kg^{-1}$ and their % ADI (% Acceptable Daily Intake) were 17.44% and 25.75%, respectively. Based on the results obtained in this study, we suggest that the residue levels of both of the fungicides on Allium victorialis are safe and these fungicides can also be used to control fungal diseases in Allium victorialis.

Chemical and Spectroscopic Characterization of Soil Humic and Fulvic Acids and Sorption Coefficient of Phenanthrene: A Correlation Study (토양 휴믹물질의 화학적.분광학적 특성에 따른 페난트린 흡착상수와의 상관성 규명에 대한 연구)

  • Lee, Doo-Hee;Lee, Seung-Sik;Shin, Hyun-Sang
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.11
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    • pp.1067-1074
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    • 2008
  • In this study, the organic carbon normalized-sorption coefficients (Koc) for the binding affinity of phenanthrene (PHE) to 16 different soil humic and fulvic acids of various origins were determined by fluorescence quenching. The humic and fulvic acids used in this study were isolated from 6 different domestic soils including Mt. Hanla soil, IHSS standard soil and peat as well as Aldrich humic acid and characterized by elemental composition, ultraviolet absorption at 254 nm, composition of main structural fragments determined by CPMAS $^{13}$C NMR. The Koc values($\times$10$^4$, L/kg C) for each of HA and FA samples were in the range of 1.48$\sim$8.65 and higher in HA compared to that of FA(3.13$\sim$8.65 vs 1.48$\sim$2.48) in the experimental condition([PHE]/[HS] = 0.02$\sim$0.2(mg/L)/(mg-OC/L), pH 6). The correlation study between the structural descriptors of humic and fulvic acids and log Koc values of phenanthrene, show that the magnitude of Koc values positively correlated with the UV$_{254}$ absorptivity([ABS]$_{254}$) and two $^{13}$C NMR descriptors (C$_{Ar-H,C}$, $\sum$C$_{Ar}$/$\sum$C$_{Alk}$), while negatively correlated with the independent descriptors of the(N+O)/C atomic ratios and $^{13}$C NMR descriptors (I$_{C-O}$/I$_{C-H,C}$). These results confirmed that the binding affinity for the hydrophobic organic compound, phenanthrene are significantly influenced by the polarity and aromaticity of soil humc and fulvic acids.

Determination of secondary aliphatic amines in surface and tap waters as benzenesulfonamide derivatives using GC-MS (Benzenesulfonamide 유도체로 GC-MS를 사용한 지표수 및 수돗물 중 2차 지방족 아민의 분석)

  • Park, Sunyoung;Jung, Sungjin;Kim, Yunjeong;Kim, Hekap
    • Analytical Science and Technology
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    • v.31 no.2
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    • pp.96-105
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    • 2018
  • This study aimed to improve the method for detecting eight secondary aliphatic amines (SAAs), so as to measure their concentrations in fresh water and tap water samples. NaOH (8 mL, 10 M) and benzenesulfonyl chloride (2 mL) were added to a water sample (200 mL), and the mixture was stirred at $80^{\circ}C$ for 30 min. An additional NaOH solution (10 mL) was added and the stirring was continued for another 30 min. The pH of the cooled mixture was adjusted to 5.5-6.0 by adding HCl (35 %), and the SAAs were extracted using dichloromethane (50 mL). This extraction was repeated once. The extract was then washed with $NaHCO_3$ (15 mL, 0.05 M) and dried over $Na_2SO_4$ (4 g). The extract was finally concentrated to 0.1 mL, of which $1{\mu}L$ was analyzed for SAAs by GC-MS. The linearity of the spike calibration curves was high ($r^2=0.9969-0.9996$). The detection limits of the method ranged from 0.01 to $0.20{\mu}g/L$, and its repeatability and reproducibility (expressed as relative standard deviation) were both less than 10 % (6.6-9.4 %). Its accuracy (measured in percentage error) ranged between 2.4 % and 6.1 %. The established method was applied to the analysis of five surface water and 82 tap water samples. Dimethylamine was the only SAA detected in all the water samples, and its average concentration was $0.79{\mu}g/L$ (range: $0.20-2.54{\mu}g/L$). Therefore, this study improved the analytical method for SAAs in surface water and tap water, and the regional and seasonal concentration distributions were obtained.

Empirical Orthogonal Function Analysis of Surface Pressure, Sea Surface Temperature and Winds over the East Sea of the Korea (Japan Sea) (한국 동해에서의 해면기압, 해수면온도와 해상풍의 경험적 직교함수 분석)

  • NA Jung-Yul;HAN Snag-Kyu;SEO Jang-Won;NOH Yi-Gn;KANG In-Sik
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.2
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    • pp.188-202
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    • 1997
  • The seasonal variability of the sea surface winds over the last Sea of Korea (Japan Sea) is investigated by means of empirical orthogonal function (EOF) analysis. The combined representation of fields of three climatic variables by empirical orthogonal functions is discussed. The eigenvectors are derived from daily sea level pressure, wind speed and 10-day mean sea surface temperature (SST) during 15 years $(1978\~1992)$. The spatial patterns of the mean pressure are characterized by the high pressure in the western part and the low pressure in the eastern part. The spatial distribution of the standard deviation (SD) of pressure are characterized by max SD of 6.6 mb near the Vladivostok, and minima along the coast of the Japan. In Vladivostok, the maxima of SD of SST and south-north wind (WV) were also occurred. The representation of fields of individual meteorological variables by EOF shows that the first mode of the west-east wind (WU) explain over $47.3\%$ of the variance and the second mode of WU represents $30\%$. Especially, the first mode of the WV explain $70.9\%$ of the variance and their time series coefficients show 1-cpy, 0.5-cpy frequency spectrum. The spatial distribution of the first mode eigenvectors of SST are characterized by maximum near Vladivostok. The combined representation of fields of several variables (pressure, wind, SST) reveals that the first mode magnitudes of the variance of the combined eigenvectors (WU-PR) are increased. By means of this result, the 1-year peak and the 6-months peak are remarkable. In the three combined patterns (wind, pressure, SST), the second mode of the eigenvector (wind) is affected by the SST. Their time coefficients of the first mode show noticeable 1-year peak. The spectral analysis of the second mode shows broad seasonal signal with the period of 4-months and a significant peak of variability at 3-month period.

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