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Alcohol Fermentation at High Temperature and the Strain-specific Characteristics Required to Endow the Thermotolerance of Sacchromyces cerevisiae KNU5377

  • Paik, Sang-Kyoo;Park, In-Su;Kim, Il-Sup;Kang, Kyung-Hee;Yu, Choon-Bal;Rhee, In-Koo;Jin, In-Gnyol
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 2005.06a
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    • pp.154-164
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    • 2005
  • Saccharomyces cerevisiae KNU5377 is a thermotolerant strain, which can ferment ethanol from wasted papers and starch at 40$^{\circ}C$ with the almost same rate as at 30$^{\circ}C$. This strain showed alcohol fermentation ability to convert wasted papers 200 g (w/v) to ethanol 8.4% (v/v) at 40$^{\circ}C$, meaning that 8.4% ethanol is acceptable enough to ferment in the industrial economy. As well, all kinds of starch that are using in the industry were converted into ethanol at 40$^{\circ}C$ with the almost same rate as at 30$^{\circ}C$. Hyperthermic cell killing kinetics and differential scanning calorimetry (DSC) revealed that exponentially growing cells of this yeast strain KNU5377 were more thermotolerant than those of S. cerevisiae ATCC24858 used as a control. This intrinsic thermotolernace did not result from the stability of entire cellular components but possibly from that of a particular target. Heat shock induced similar results in whole cell DSC profiles of both strains and the accumulation of trehalose in the cells of both strains, but the trehalose contents in the strain KNU5377 were 2.6 fold higher than that in the control strain. On the contrary to the trehalose level, the neutral trehalase activity in the KNU5377 cells was not changed after the heat shock. This result made a conclusion that though the trehalose may stabilize cellular components, the surplus of trehalose in KNU5377 strain was not essential for stabilization of whole cellular components. A constitutively thermotolerant yeast, S. cerevisiae KNU5377, was compared with a relatively thermosensitive control, S. cerevisiae ATCC24858, by assaying the fluidity and proton ATPase on the plasma membrane. Anisotropic values (r) of both strains were slightly increased by elevating the incubation temperatures from 25$^{\circ}C$ to 37$^{\circ}C$ when they were aerobically cultured for 12 hours in the YPD media, implying the membrane fluidity was decreased. While the temperature was elevated up to 40$^{\circ}C$, the fluidity was not changed in the KNU5377 cell, but rather increased in the control. This result implies that the plasma membrane of the KNU5377 cell can be characterized into the more stabilized state than control. Besides, heat shock decreased the fluidity in the control strain, but not in the KNU5377 strain. This means also there's a stabilization of the plasma membrane in the KNU5377 cell. Furthermore, the proton ATPase assay indicated the KNU5377 cell kept a relatively more stabilized glucose metabolism at high temperature than the control cell. Therefore, the results were concluded that the stabilization of plasma membrane and growth at high temperature for the KNU5377 cell. Genome wide transcription analysis showed that the heat shock responses were very complex and combinatory in the KNU5377 cell. Induced by the heat shock, a number of genes were related with the ubiquitin mediated proteolysis, metallothionein (prevent ROS production from copper), hsp27 (88-fold induced remarkably, preventing the protein aggregation and denaturation), oxidative stress response (to remove the hydrogen peroxide), and etc.

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A Field Survey on Concentration of Odor Compounds in Pig Buildings and Boundary Areas (돈사 내 및 부지경계에서 악취물질 발생 조사 연구)

  • Yoo Yong-Hee;Kim Tae-Il;Jeong Jong-Won;Gwak Jeong-Hun;Choi Hee-Chul;Song Jun-Ik;Yang Chang Bum;Jang Young-Kee;Kim Ho-Jung;Song Ki-Pong
    • Journal of Animal Environmental Science
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    • v.11 no.1
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    • pp.45-54
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    • 2005
  • A field survey was conducted to determine the concentration of odor compounds from pig buildings and that were 20 meters within the boundary area. The odor compounds were measured from large, medium and small farms with enclosed and open housing systems and slurry and sawdust manure fermentation treatment methods. Among the odor compounds investigated, ammonia ($NH_3$) had the highest concentration at 0.9 ${\~}$ 21.0 ppm followed by Hydrogen Sulfide($H_2S$) with a wide variation concentration of 51.9 ${\~}$ 6,712.4 pub, Uethylmercaptan($CH_3SH$) with non-detectable (N. D.) ${\~}$ 12.9 ppb, Dimethylsulphide($(CH_3)_2S$), with N. D. ${\~}$ 5.2 ppb and Dimethyldisulphide($(CH_3)_2S_2$) with N. D. ${\~}$ 2.6 ppb. Considering the prevailing wind direction and air velocity ranging from 0.23 to 0.73 m/s within the boundary area, the odorous matters; $NH_3$, $H_2S$, $CH_3SH$, $(CH_3)_2S_2$ and $(CH_3)_2S$ were 0.2${\~}$4.5 ppm, 0.01 ${\~}$0.06 ppb, N. D. ${\~}$0.009ppb, N. D.${\~}$0.002ppb and N. D. for $(CH_3)_2S$ respectively. These findings suggested that the Odor compounds $(CH_3)_2S_2$ had the lower detection in the boundary area whilst $(CH_3)_2S$ had no detection level within a 20-meter distance only. However, with these results odor compounds from pig buildings has to be further investigated under more controlled environmental factors.

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Quantitative Measurement of Carbon Dioxide Consumption of a Whole Paprika Plant (Capsicum annumm L.) Using a Large Sealed Chamber (대형 밀폐 챔버를 이용한 파프리카(Capsicum annumm L.) 개체의 이산화탄소 소비량 측정 및 정량화)

  • Shin, Jong-Hwa;Ahn, Tae-In;Son, Jung-Eek
    • Horticultural Science & Technology
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    • v.29 no.3
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    • pp.211-216
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    • 2011
  • This study was carried out to clarify precise $CO_2$ demands of paprika plants (Capsicum annumm L.) by measuring photosynthesis rates of the leaves in high, low positions, and the $CO_2$ consumption of a whole plant in a large sealed chamber. A photosynthesis measuring system (LI-6400) was used to measure the photosynthetic rates of the leaves located in different positions. A large sealed chamber that can control inside environmental factors was developed for measuring $CO_2$ consumption by a whole paprika plant. With increase of radiation, photosynthetic rates of the leaves in higher position became larger than those in lower position. The $CO_2$ consumption by the plant was estimated by using decrement of $CO_2$ concentration from initial level of 1500 ${\mu}mol{\cdot}mol^{-1}$ in the chamber with increase of integrated radiation. A regression model for estimating $CO_2$ consumption by the plant (leaf area = 7,533.4 $cm^2$) was expressed with integrated radiation (x) and was suggested as $y=-0.06234+3.671^*x/(2.589+x)$ ($R^2=0.9966^{***}$). The photosynthetic rate of the whole plant measured in the chamber was 3.4 ${\mu}mol\;CO_2{\cdot}m^{-2}{\cdot}s^{-1}$ under 300 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ light intensity, which is in-between photosynthetic rates of the leaves in high and low positions. For this reason, some differences between required and supplied $CO_2$ amounts in greenhouses might occur when depending too much on photosynthetic rates of leaves. Therefore, we can estimate more accurately $CO_2$ amount required in commercial greenhouses by using $CO_2$ consumption model of a whole plant obtained in this study in addition to leaf photosynthetic rate.

A Study on Performance and Achievement of Village Health Workers in Rural Primary Health Care Program (농촌 일차 보건사업에 있어서 마을건강원 업무량 및 업적에 관한 연구)

  • Hur, Dal-Young;Lee, Myoung-Sook;Yum, Yong-Tae;Kim, Soon-Duck
    • Journal of agricultural medicine and community health
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    • v.12 no.1
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    • pp.36-53
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    • 1987
  • It is utmostly important to establish the efficient fitable way of peoples' active participation in primary health care especially in the areas where the public or governmental service input for the basic health care is insufficient like as in rural areas of Korea. In light of above reason, this study focused mainly on the evaluation of roles and activities of village health workers (VHWs) who were selected from grass- root level of village people in order to derive further motivation for active participation. This is believed to be a sort of feedback mechanisms. Actually, the authors collected the activity reports of VHWs who had been devoting themselves in the primary health care services of Jeomdong Area, of Yeoju Gun one of Korea University Community Health Action Programmes and survey record on the VHWs activity from correspondent people. 1 hose data were analyzed through computer programmed package. The activities performed by VHWs were limited to the performance in 1985 for conveniance. The summarized results were as follows; 1) General characteristics of VHWs. Among a total of 28 VHWs in the area, about 39.3g of them have been replaced up to the date since the implementation in 1983, because of moving out, occupational employment and of others. The age of majority (75.0%) lied between the range of 30-50, and educational background of 67.9% belonged to category of primary school graduation, about 50% of them experienced to be or were also entiled "chief of women club" of corresponding villages. 2) Work-load of VHWs. Each VHW was assigned for tasks of health care for average 55 households of 248 persons. They shared approximately 6 days a month for the activity in average and it covered 17 cases of basic health care in a month. A half of the VHWs performed home visits irregularly without solidified schedule. 3) Work performance analysis. Informations collected through VHWs were compared with data from official vital registration at local administration center "Myon Office" in 1985. VHWs collected 100.8 of new born, 116.2 of death, 58.3 of move in and 74.8 of move out in comparison with 100.0 of official registration each. Pregnant women of 79.8% of mothers among the total pregnancy of 94 which were confirmed as normally delivered or aborted cases by all means afterwards had been detected by VHWs as being pregnant and all of them received some of antenatal cares by VHWs. All(100%) of delivered women were detected by VHWs through home visits and they were cared postnatally. Whereas, according to the records of birth registration, the places of delivery were clinic in 33.7%, and mother's home in 66.3%, VHWs reported them to be clinic in 48.9%, midwifery in 20.2%. It was cleared that most of misinformation was caused by uncautious filling of birth registration at notification. Among the total of 717 eligible women under age 44 years, family planning status of 92.6% was reported by VHWs confirming practice of control to be 70.8% of reported fertile women. 4) Attitude of VHW on the roles and functions. Although 92.0% of VHWs expressed VHWs to be worthwhile, only 52.0% of them had dignity and satisfaction in their activity and 44.0% of them had passive attitude of working saying they followed direction regardlessly. Concerning difficulties in performance as a VHW, 60.7% of them pointed out lacking of medical and health related knowledge by themselves. Still, 64.0% of them thought visiting unfamilier house to be awful and 40.0% complained forms of activity to be difficult and hard. It was also revealed that 56.6% confessed lack of interest on community health service itself. Most of VHWs needed more educational training especially on clinical fields such as cares of gynecological diseases, hypertension, diabetes, and other chronic diseaes of the aged. Regular on-the-job basic trainings were said to be needed twice a year.

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The Study on Improvement of the Digital Transformation of Small and Medium-Sized Manufacturing Industries through Foreign Countries (주요국 정책을 통한 중소 제조기업의 디지털 전환 추진 방향 모색)

  • An, Jung-in
    • Journal of Venture Innovation
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    • v.5 no.4
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    • pp.109-115
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    • 2022
  • As the 4th industrial revolution progresses, foreign countries are promoting smart manufacturing innovation through digital transformation as a priority task early on to secure a competitive edge in the manufacturing industry. In response, the Korean government is also promoting a policy to enhance the competitiveness of small and medium-sized manufacturing companies by promoting digital transformation in the corporate sector to meet the global trend of the 4th industrial revolution era. Manufacturing powerhouses such as Germany and Japan see manufacturing as a key sector in digital transformation and are leading related policies, while emerging countries such as China are also promoting manufacturing innovation strategies such as building digital infrastructure and creating a digital innovation ecosystem. Korea is promoting the 'Korean-style smart factory dissemination and expansion strategy' by transforming Germany's manufacturing innovation strategy for smart factory supply to suit the domestic situation. However, the policy to supply smart factories so far has been conducted with support from individual companies under the leadership of the government, and most of the smart factories are at the basic level, and it is evaluated that there are limitations such as the lack of manpower to operate smart factories. In addition, while the current policy focuses on expanding the supply of smart factories in SMEs, it is necessary to establish a smart manufacturing system through linkages between large and small businesses in order to achieve the original goal of establishing a smart manufacturing system. Therefore, it can be said that from the standpoint of small and medium-sized enterprises (SMEs), who are consumers of smart factories, it can be said that the digital transformation policy can achieve the expected results only when appropriate incentives are provided for the introduction of smart factories in a situation where management resources such as funds, technology, and human resources are lacking. In addition, it is judged that the uncertainty of the performance of digital investment always exists, and as long as large and small companies are maintained as an ecosystem of delivery and subcontracting, there is very little incentive for small and medium-sized manufacturing companies to voluntarily invest in or advance digital transformation. Therefore, the digital transformation policy of small and medium-sized manufacturing companies in the future has practical significance in that it suggests that there is a need to seek ways to attract SMEs' digital-related voluntary investment.

Crystallographic Study on the Selectivity and Distribution of Sr2+ Ions Within Zeolite A In the Presence of Competing Na+ Ions in Aqueous Exchange Solution (Na+ 경쟁이온이 존재하는 수용액에서 Zeolite A 내 Sr2+ 이온의 선택성 및 분포에 관한 결정학적 연구)

  • kim, Hu Sik;Park, Jong Sam;Lim, Woo Taik
    • Korean Journal of Mineralogy and Petrology
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    • v.35 no.1
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    • pp.41-50
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    • 2022
  • To study the properties of Sr2+ exchange into zeolite A with increasing the molar concentration of Na+ in given exchange solution, four single crystals of fully dehydrated Sr2+- and Na+- exchanged zeolite A were prepared by the bath method using mixed ion-exchange solutions. The Sr(NO3)2:NaNO3 molar rations of the ion exchange solution were 1:1(crystal 1), 1:100(crystal 2), 1:250(crystal 3), and 1:500 (crystal 4), respectively, with a total concentration of 0.05 M. The single-crystals were then vacuum dehydration at 623 K and 1×10-4 Pa for 2 days. Their single-crystal structures were determined by single-crystal synchrotron X-ray diffraction techniques in the cubic space group Pm3-m, at 100(1) K, and were then refined to the final error indices of R1/wR2=0.047/0.146, 0.048/0.142, 0.036/0.128, and 0.040/0.156 for crystals 1, 2, 3, and 4, respectively. In crystals 1 and 2, the 6 Sr2+ ions are found at three different crystallographic sites. In crystal 3, 1 Sr2+ and 10 Na+ ions are found in large cavity and sodalite unit. In crystal 4, only 12 Na+ ions occupy three equipoints. The degree of Sr2+ ion-exchange decreased sharply from 100 to 16.7 to 0% as the initial Na+ concentration increase and the Sr2+ concentration decrease. In addition, the unit cell constant of the zeolite framework decreased with this lower level of Sr2+ exchange.

Production and Quality Parameters of Oat Grown in Conventional/Organic Farming

  • Petr Konvalina;Ivana Capouchova
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.19-19
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    • 2022
  • Hulled and naked oat is a perspective crop for the low input production systems due to its low requirements for soil quality and nutrition. Oats have good competitive ability against weeds and can provide appropriate yield in organic farming in comparison with other cereal species such as wheat or barley. It is a perspective crop from the point of view of use in the food industry too. The aim of our study was to compare the production and quality parameters of naked and hulled oat grown in both organic (OF) and conventional fields (CF). Small plot trials were conducted in two locations in the Czech Republic (České Budějovice, Prague) for four years (2018-2021) in two production systems (OF, and CF). We used four varieties of hulled oat (Korok, Kertag, Raven, Seldon) and one variety of naked oat (Patrik). During the vegetation, agronomically important data were recorded. After harvest samples were processed in the laboratory and analyzed selected quality parameters of grain dry matter (the protein content was determined by the Kjeldahl method, starch content in grain according to Ewers, fat content in grain dry matter by the modified method according to Soxhlet, and ash content in grain dry matter). The data were evaluated using the program STATISTICA version 13.2, StatSoft, Inc., California, USA. It is clear from the results that the number of panicles before the harvest was influenced by the location, cultivation system, year, and, to a lesser extent, the influence of the variety. The number of panicles in OF averaged 340 per square meter, which was 90% of the value of CF. For thousand grain weight (TGW), a significantly predominant effect of year was found. The independent effect of location on TGW was statistically not significant. Grain yield was predominantly influenced by cultivation system and location. In OF, it reached an average of 3.97 t.ha-1, which was 75% of the yield of CF. As part of the evaluation of the basic grain quality indicators, the content of protein, starch, fat, and ash in the dry matter of the grain was evaluated. The content of protein in the dry matter of the grain was predominantly influenced by year, followed by the influence of the variety and a fairly comparable influence of the cultivation system and locality. On average, it achieved 16.05% in OF and 17.01% in CF. The starch content was then related to the protein content, where as a result of the lower protein content in the grain of OF oats, the content of starch and fat was on the contrary increased. The year turned out to be the most significant factor, affecting both the starch content in the dry matter of the grain and the fat content. This was followed again by a fairly comparable influence on the cultivation system and locality. The influence of the cultivation system and location was not statistically significantly applied in the case of ash content in dry matter. Based on our results we can propose both types of oat (hulled and naked) as perspective crops for OF. An organic farmer can expect to achieve stable yields which, in less favorable conditions for the production of cereals in the OF, may be close to the level of conventional yields. In the future, it will be important to change agrotechnology in OF and increase oat yield because this crop has a good potential to grow in areas with low nitrogen input or less fertile soil.

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Development and Application Effect of Design-based STEAM Program for Boosting the Career Consciousness of 5~6th Grade Elementary School Students for Natural Sciences and Engineering (이공계 진로의식 신장을 위한 초등 5~6학년용 설계기반 미래 유망직업 STEAM 프로그램 개발 및 적용 효과)

  • Lim, Yoo-Na;Min, Bu-Ja;Hong, Hoo-Jo
    • Journal of The Korean Association For Science Education
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    • v.35 no.1
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    • pp.73-84
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    • 2015
  • In this research, two careers connectable with the contents of the curriculum of fifth to sixth grade elementary school students were selected among other promising future careers in biotechnology and medical engineering fields. 'Design-based promising future career STEAM program' was developed and its validity and effectiveness were verified. Reflecting recent issues, and complying with the STEAM standard (frame) instructional materials were developed through group deliberations for nine months, based on the achievement standards through an analysis of subject curriculum revised in 2009. This was prepared so that students are able to experience biotechnology and medical engineering related careers in a simulational form emphasized with creative design to make them prefer natural sciences and engineering careers and draw their interests and recognition of the relevant careers under the two disciplines. As a result of such application to STEAM Leader School students at the verification stage of the program, the contents and level of the program were verified suitable, receiving favorable reviews. And as a result of applying the developed program on other elementary school students, it was discovered that significant improvements were found in their career consciousness. Through this research, it was suggested that there is a need for a simplification of the curriculum content standards, a provision of 'standard for integration,' development of teachers' ability in reconstituting or organizing the STEAM and proceeding classes, continuous long-term support to see the effects of a policy or a program, and a reinforcement of career education integrated in the curriculum.

Influence of Gypsum, Popped Rice Hulls and Zeolite on Contents of Cation in Reclaimed Tideland Soils in Mangyeong (새만금 간척지에서 석고, 팽화왕겨 및 제올라이트 처리가 토양 중 양이온 함량에 미치는 영향)

  • Baek, Seung-Hwa;Lee, Sang-Uk;Kim, Dae-Geun;Heo, Jong-Wook;Kim, Seong-Jo
    • Korean Journal of Environmental Agriculture
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    • v.27 no.4
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    • pp.321-327
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    • 2008
  • Soil conditioner, such as $CaSO_4{\cdot}2H_2O$ (gypsum), popped rice hulls (PRH), and PRH with zeolite, were treated to the silt loam of Mangyeong in Saemangeum tideland reclaimed as 1550 (G1), 3100 (G2) and 6200 (G3) of gypsum kg/10 a, 1000(H1), 2000(H2), and 3000 (H3) of PRH kg/10 a, and 200 (HZ1), 400 (HZ2), 800 (HZ3) of zeolite kg/10 a added to 1500 PRH kg/10 a, respectively, each year until 2006 from 2004 for soil aggregation. Under these conditions with growing bermuda grass (Cynodon dactylon) it was analyzed cations in soil, such as $K^+$, $Na^+$, $Mg^{2+}$, and $Ca^{2+}$, at 60, 90, and 120 days after treatment (DAT) to research how soil conditioners influenced to change those contents in soils, respectively. The change of cations in soil was almost the same things as fine sandy loam that gypsum treated decreased remarkably contents of $K^+$, $Na^+$, $Mg^{2+}$ in soil. The change of $K^+$ content in soil by continuous using soil conditioners was gradually decreased in the order of 2004>2005>2006, regardless of the sorts and levels of soil treated conditioners, and $K^+$ content was high in the order of gypsum$Na^+$ content was high in the order of gypsum$Mg^{2+}$ content in soil was increased in the order of gypsum$Ca^{2+}$ content in soil was remarkably increased with continuous treatment of gypsum, and its level was in the order of 2004<2005<2006.

The Pattern Analysis of Financial Distress for Non-audited Firms using Data Mining (데이터마이닝 기법을 활용한 비외감기업의 부실화 유형 분석)

  • Lee, Su Hyun;Park, Jung Min;Lee, Hyoung Yong
    • Journal of Intelligence and Information Systems
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    • v.21 no.4
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    • pp.111-131
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    • 2015
  • There are only a handful number of research conducted on pattern analysis of corporate distress as compared with research for bankruptcy prediction. The few that exists mainly focus on audited firms because financial data collection is easier for these firms. But in reality, corporate financial distress is a far more common and critical phenomenon for non-audited firms which are mainly comprised of small and medium sized firms. The purpose of this paper is to classify non-audited firms under distress according to their financial ratio using data mining; Self-Organizing Map (SOM). SOM is a type of artificial neural network that is trained using unsupervised learning to produce a lower dimensional discretized representation of the input space of the training samples, called a map. SOM is different from other artificial neural networks as it applies competitive learning as opposed to error-correction learning such as backpropagation with gradient descent, and in the sense that it uses a neighborhood function to preserve the topological properties of the input space. It is one of the popular and successful clustering algorithm. In this study, we classify types of financial distress firms, specially, non-audited firms. In the empirical test, we collect 10 financial ratios of 100 non-audited firms under distress in 2004 for the previous two years (2002 and 2003). Using these financial ratios and the SOM algorithm, five distinct patterns were distinguished. In pattern 1, financial distress was very serious in almost all financial ratios. 12% of the firms are included in these patterns. In pattern 2, financial distress was weak in almost financial ratios. 14% of the firms are included in pattern 2. In pattern 3, growth ratio was the worst among all patterns. It is speculated that the firms of this pattern may be under distress due to severe competition in their industries. Approximately 30% of the firms fell into this group. In pattern 4, the growth ratio was higher than any other pattern but the cash ratio and profitability ratio were not at the level of the growth ratio. It is concluded that the firms of this pattern were under distress in pursuit of expanding their business. About 25% of the firms were in this pattern. Last, pattern 5 encompassed very solvent firms. Perhaps firms of this pattern were distressed due to a bad short-term strategic decision or due to problems with the enterpriser of the firms. Approximately 18% of the firms were under this pattern. This study has the academic and empirical contribution. In the perspectives of the academic contribution, non-audited companies that tend to be easily bankrupt and have the unstructured or easily manipulated financial data are classified by the data mining technology (Self-Organizing Map) rather than big sized audited firms that have the well prepared and reliable financial data. In the perspectives of the empirical one, even though the financial data of the non-audited firms are conducted to analyze, it is useful for find out the first order symptom of financial distress, which makes us to forecast the prediction of bankruptcy of the firms and to manage the early warning and alert signal. These are the academic and empirical contribution of this study. The limitation of this research is to analyze only 100 corporates due to the difficulty of collecting the financial data of the non-audited firms, which make us to be hard to proceed to the analysis by the category or size difference. Also, non-financial qualitative data is crucial for the analysis of bankruptcy. Thus, the non-financial qualitative factor is taken into account for the next study. This study sheds some light on the non-audited small and medium sized firms' distress prediction in the future.