• 한국광학회지
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• 제24권2호
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• pp.86-91
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• 2013

본 논문에서는 근접장-분자반응 간의 중첩을 이용한 표면 플라스몬 공명 (SPR) 바이오센서의 측정감도 평가방법을 연구하였다. 전달행렬 방법을 사용하여 다양한 형태의 중첩적분으로 정의된 광학자취 값을 계산하였고, 샌드위치 및 역샌드위치 면역글로뷸린 (IgG) 어세이에 대해서 실험적으로 측정된 수치와 비교하였다. 이론 및 실험적인 결과와의 비교를 통하여 접선 방향의 전기장을 사용한 광학자취의 경우 그 연속성으로 말미암아 가장 높은 상관계수를 얻을 수 있었으며 이때 광학자취와 측정감도 사이에 97% 이상의 높은 상관계수가 존재함을 보았다. 이러한 상관관계는 SPR 바이오센서의 측정 감도에 관한 메커니즘을 분명하게 설명하며, 분자 스케일 감도를 가지는 SPR 바이오센서 개발에 기여하게 될 것이다.

• Journal of Ginseng Research
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• 제45권2호
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• pp.218-227
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• 2021

Background: Panax ginseng is one of the most important medicinal plants and is usually harvested after 5 to 6 years of cultivation in Korea. Heavy metal (HM) exposure is a type of abiotic stress that can induce oxidative stress and decrease the quality of the ginseng crop. Siderophore-producing rhizobacteria (SPR) may be capable of bioremediating HM contamination. Methods: Several isolates from ginseng rhizosphere were evaluated by in vitro screening of their plant growth-promoting traits and HM resistance. Subsequently, in planta (pot tests) and in vitro (medium tests) were designed to investigate the SPR ability to reduce oxidative stress and enhance HM resistance in P. ginseng inoculated with the SPR candidate. Results: In vitro tests revealed that the siderophore-producing Mesorhizobium panacihumi DCY119^T had higher HM resistance than the other tested isolates and was selected as the SPR candidate. In the planta experiments, 2-year-old ginseng seedlings exposed to 25 mL (500 mM) Fe solution had lower biomass and higher reactive oxygen species level than control seedlings. In contrast, seedlings treated with 10⁸ CFU/mL DCY119^T for 10 minutes had higher biomass and higher levels of antioxidant genes and nonenzymatic antioxidant chemicals than untreated seedlings. When Fe concentration in the medium was increased, DCY119^T can produce siderophores and scavenge reactive oxygen species to reduce Fe toxicity in addition to providing indole-3-acetic acid to promote seedling growth, thereby conferring inoculated ginseng with HM resistance. Conclusions: It was confirmed that SPR DCY119^T can potentially be used for bioremediation of HM contamination.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2003년도 동계 학술대회 논문집
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• pp.200-208
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• 2003

미생물수를 측정하는 가장 정확하고 보편적인 방법은 배양법이다. 그러나 배양법은 많은 시간과 노력이 소요되기 때문에 현장에서 실시간에 준하는 측정법으로 사용하기에는 원천적으로 한계를 가지고 있다. 이러한 한계를 극복하기 위해서는 실시간(real-time) 또는 준실시간(near real-time)으로 미생물을 검출할 수 있는 기술이 요구된다. 표면플라즈몬공명(surface plasmon resonance: SPR)은 생물분자 또는 미생물을 실시간 또는 준실시간으로 검출할 수 있는 센서로서 특이성(specificity)과 정확도(accuracy) 측면에서 일찍이 큰 관심을 받아왔다. 1902년, Wood(1902)는 반사 회절격자를 사용하여 연속광원의 스펙트럼을 관찰한 결과, 회절광 스펙트럼에서 어두운 좁은 밴드를 발견하였으며, Fano(1941)는 이 현상이 surface plasma waves와 관련이 있음을 이론적으로 밝혔다. (중략)

• 전기학회논문지
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• 제56권1호
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• pp.156-160
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• 2007

Surface plasmon resonance(SPR) has been widely studied for biological and chemical sensing applications. The present study conducts numerical simulation for the single and bimetallic layer SPR configurations by using the multiple beam interference matrix(MBIM) method to investigate the influence of wave interference and complex refractive indices of materials on optical characteristics such as reflectance and optical phase shift which are used for sensing. First, calculated reflectances are compared with experimental data for validation. In addition, in the single film structures this study finds out the appropriate film thicknesses with minimum reflectance for cases of gold film and silver film. For a bimetallic silver-gold film structure, in particular, the bimetallic film thicknesses that has the minimum reflectance are found 36 nm for silver and 5 nm for gold. From the results, the use of phase shift would be useful compared to reflectance in determining the SPR configuration because the phase shift becomes more sensitive than reflectance.

• International Journal of Automotive Technology
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• 제7권5호
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• pp.565-570
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• 2006

The development of a light-weight vehicle is in great demand for enhancement of fule efficiency and dynamic performance. The vehicle weight can be reduced effectively by using lightweight materials such as aluminum and magnesium. However, if such materials are used in vehicles, there are often instances when different materials such as aluminum and steel need to be joined to each other. The conventional joining method, namely resistance spot welding, cannot be used in joining different materials. Self-piercing rivet(SPR) and adhesive bonding, however, are good alternatives to resistance spot welding. This paper is concerned with the crushing test of double hat-shaped member made by resistance spot welding, SPR and adhesive bonding. Various parameters of crashworthiness are analyzed and evaluated. Based on these results, the applicability of SPR and adhesive bonding are proposed as an alternative to resistance spot welding.

• 전기학회논문지
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• 제59권5호
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• pp.922-926
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• 2010

We have investigated the grating coupled surface plasmon resonance (GC-SPR) sensors using ZnO nano-grating structures to enhance the sensitivity of an SPR sensor. The GC-SPR sensors were analyzed using the finite-difference time-domain method. The optimum resonance angles of 49 degrees are obtained in the 150 nm wide grating structure with a period of 300 nm for the ZnO thickness of 30 nm. Then, the ZnO nano-grating patterns were fabricated by using laser interference lithography. The measured resonance angle of nano-grating patterns was around 49 degrees. Here, an enhanced evanescent field is obtained due to the surface plasmon on the edge of the bandgap when the ZnO grating structures are used to excite the surface palsmon.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2008년도 춘계학술대회논문집
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• pp.442-448
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• 2008

This paper presents an approach to define an optimal piezoactuator length to actively control structural vibration. The optimal ratio of the piezoactuator length against beam length when a pair of piezoceramic actuator and accelerometer is used to suppress unwanted vibration with direct velocity feedback (DVFB) control strategy is not clearly defined so far. It is well known that direct velocity feedback (DVFB) control can be very useful when a pair of sensor and actuator is collocated on structures with a high gain and excellent stability. It is considered that three different collocated pairs of piezoelectric actuators (20, 50 and 100 mm) and accelerometers installed on three identical clamped-clamped beams (300 * 20 * 1 mm). The response of each sensor-actuator pair requires strictly positive real (SPR) property to apply a high feedback gain. However the length of the piezoactuator affects SPR property of the sensor-actuator response. Intensive simulation and experiment shows the effect of the actuator length variation is strongly related with the frequency range of SPR property. A shorter actuator gave a wider SPR frequency range as a longer one had a narrower range. The shorter actuator showed limited control performance in spite of a higher gain was applied because the actuation force was relatively small. Thus an optimal length ratio (actuator length/beam length) was suggested to obtain relevant performance with good stability with DVFB strategy. The result of this investigation could give important information in the design of active control system to suppress unwanted vibration of smart structures with piezoelectric actuators and accelerometers.

• Journal of the Optical Society of Korea
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• 제11권2호
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• pp.55-58
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• 2007

We propose a systematic method for design of fiber-optic surface plasmon resonance (SPR) sensors. We used rigorous coupled wave analysis (RCWA) for analysis of the transmission spectrum, and the (1+1) evolution strategy (ES) was employed as an optimization tool. The simulation results show that the optimization method presented here is very useful in designing fiber-optic SPR sensor for strain and temperature measurement. This algorithm can be extended to another objective function with other weighting factors and optical parameters.

• Bulletin of the Korean Chemical Society
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• 제33권1호
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• pp.100-104
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• 2012

Thrombin is a serine protease that catalyzes the conversion of soluble fibrinogen to insoluble fibrin, and thus induces physiological and pathological blood coagulation. Therefore, it is important to detect thrombin in blood serum for purposes of diagnosis. To achieve this goal, it has been suggested that a 15-mer aptamer strongly binds with thrombin to form a G-quartet structure of the aptamer. Generally, 5'-end thiol-functionalized aptamer has been used as an anti-thrombin binder. Herein, we evaluate the possibility of utilizing a 3'-SH aptasensor for thrombin detection using SPR spectroscopy, and compare the enhancement of the electrochemical signal of the thrombin-aptamer bound on a porous gold substrate. Although the two aptamers have similar configurations, in SPR analysis, the 3'-SH aptamer was a effective aptasensor as well as 5'-SH aptamer. Results from electrochemical analysis showed that the porous gold substrate acted as a good substrate for an aptasensor and demonstrated 5-fold enhancement of current change, as compared to gold thin film.

• Journal of Biosystems Engineering
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• 제29권4호
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• pp.323-328
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• 2004

A surface plasmon resonance (SPR) sensing system, which belonged to a family of thin film refractometry-based sensors, was fabricated to measure the alcohol contents in alcoholic beverages. In this study, a SPR owe was obtained by a photodiode array with 128 pixels and transformed into a refractive index which was calculated by means of the first moment of values below a baseline of 0.85. When the sensing system was applied to Soju, Cheonju, Igwaduju and Tacju highly linear relationships between the refractive index by SPR and the contents of alcohol were obtained. That is, the coefficients of determination in their linear models were 0.992, 0.933, 0.918 and 0.954, respectively. Meanwhile, the effect of sample preparations on the accuracy of measurement was analyzed. As for Soju and Tacju, the best calibration equations were obtained when no sample preparation was applied. The best calibration models for measurement of Cheonju and Igwaduju were obtained through the samples filtered by $C_{18}$ disks.