• 한국측량학회지
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• 제32권6호
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• pp.599-606
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• 2014

Since the accuracy of Global Positioning System (GPS)-based vehicle positioning system is significantly degraded or does not work appropriately in the urban canyon, the integration techniques of GPS with Inertial Navigation System (INS) have intensively been developed to improve the continuity and reliability of positioning. However, its accuracy is degraded as INS errors are not properly corrected due to the GPS signal blockage. Recently, the image-based positioning techniques have been started to apply for the vehicle positioning for the advanced in processing techniques as well as the increased the number of cars installing the camera. In this study, Single Photo Resection (SPR), which calculates the camera exterior orientation parameters using the Ground Control Points (GCPs,) has been integrated with the INS/GPS for continuous and stable positioning. The INS/GPS/SPR integration was implemented in both of a loosely and a tightly coupled modes, based on the Extended Kalman Filter (EKF). In order to analyze the performance of INS/SPR integration during the GPS outage, the simulation tests were conducted with a consideration of factors affecting SPR performance. The results demonstrate that the accuracy of INS/SPR integration is depended on magnitudes of the GCP errors and SPR processing intervals. Additionally, the simulation results suggest some required conditions to achieve accurate and continuous positioning, used the INS/SPR integration.

• Journal of Microbiology and Biotechnology
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• 제17권1호
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• pp.81-88
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• 2007

The sprC gene encodes Streptomyces griseus protease C (SGPC), a bacterial chymotrypsin-like serine protease. Because the published data on sprC was not complete, we cloned and analyzed a new DNA fragment spanning downstream to upstream of the sprC gene from S. griseus IFO13350. The cloned 2.3-kb DNA fragment was placed on a high-copy number plasmid and introduced into Streptomyces lividans TK24. Chymotrypsin activity of the transformant was 8.5 times higher than that of the control after 3 days of cultivation and stably maintained until 9 days of cultivation, which dearly indicated that the cloned 2.3-kb fragment contained the entire sprC gene with its own promoter. When the same construct was introduced in the S. griseus IFO13350 (wild strain) and its two mutant strains in the A-factor regulatory cascade, ${\Delta}adpA$ and HO1, the chymotrypsin activity increased fivefold only in the ${\Delta}adpA$ strain. Transcriptional analysis based on RT-PCR revealed that the sprC gene is normally transcribed in both strains; however, earlier transcription was observed in the wild strain compared with the ${\Delta}adpA$ strain. A gel mobility shift assay showed that the AdpA protein did not bind to the promoter region of sprC. All these data clearly indicate that the expression of sprC is not dependent on the AdpA protein, but is distinctly regulated from other chymotrypsin genes composing an AdpA regulon. Earlier morphological differentiation was observed in S. lividans TK24, and S. griseus IFO13350 and HO1, transformed with the expression vector. The transformant of S. griseus ${\Delta}adpA$ formed markedly larger colonies. Antisense repression of sprC resulted in severe decrease of chymotrypsin activity, down to one-third of the control, and delayed morphological differentiation. All these data suggest that SGPC is related to normal morphogenesis in S. griseus.

• 한국정보처리학회논문지
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• 제4권2호
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• pp.409-429
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• 1997

소프트웨어를 개발하고 운영하는 동안 직면하는 문제들은 소프트웨어나 하드웨어 또는 운영의 결함에 기인한다. 이들의 다양성 문제, 문제들을 조정하고 체계적으로 원인을 규명하기 위한 문제 발생의 규명 및 수정 작업을 중심적으로 관리하는 시스템이 요구 되고 있다.본 논문에서는 소프트웨어 개발 수명 주기(Softwate Development Life Cycle :SDIC)에서 획득할 수 있는 결함 데이터(Fault Data) 프로세스 데이터(Precess Data) 프로덕트 데이터(Preduct Data)를 수집하고 분석하기 위한 소프트웨어 문제 보고서 (Software Change Report:SCR)를 처리하는 SPR/SCR 관리시스를 개발하고 적용하였다. SPR/SCR 관리 시스템의 목적은 4가지가 있다. 첫째로 모든 소프트웨어 결함들을 문서 화하고, 수정하며 무시않는다. 둘째로 SPR의 타당성에 대해 평가된다. 셋째로 SPR의 진행 상태를 개발자 또는 사용자에게 피드백한다., 넷째로 소프트웨어 품질과 신뢰성을 예측하고 측정하기 위한 기본 자료를 제공한다. 이들 목표는 SRE/SCR 관리시스템의 적용에 의해 충족시킬 수 있다. 또한 소프트웨어 신뢰성의 달성 비욜, 테스트의 종료기준, 릴 리 즈 시기의 예측, 효율적인 개발 관리에 반영될 수 있다.

• 한국항공우주학회지
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• 제39권5호
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• pp.442-450
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• 2011

회전 블레이드의 모션을 측정하기 위해 스테레오 패턴 인식 (stereo pattern recognition, SPR) 기법을 적용한 측정 시스템을 구성하고 실험을 수행하였다. 시스템 요구사항을 만족시키는 스테레오 카메라를 확보하여 SPR 기반의 측정 시스템을 준비하였다. 시스템의 측정불확도를 계산하기 위한 일련의 실험을 통해, 본 SPR 시스템이 2m~4.2m의 측정 거리에서 0.2mm 이내의 표준불확도를 가짐을 확인하였다. 이를 이용하여 계산한 블레이드 모션의 합성표준불확도는, 리드-래그, 플래핑, 비틀림 운동에 대해 각각 0.296mm, 0.209mm, $0.238^{\circ}$ 이었다. 본 SPR 시스템을 한국항공우주연구원의 소형로터 시험장치에 설치하고 회전속도와 콜렉티브 피치각을 각각 360rpm, 589rpm, 그리고 $0^{\circ}$, $4^{\circ}$, $6^{\circ}$로 바꾸어가며, 지상 제자리비행 조건에서 블레이드의 모션과 탄성 변형을 성공적으로 측정하였다. Higher Harmonic Control Aeroacoustic Rotor Test -II 에서 사용한 측정 시스템과의 비교를 통하여 본 시스템의 장점을 분석하였다.

• Journal of Microbiology and Biotechnology
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• 제16권2호
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• pp.312-317
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• 2006

The chromosomal sprD gene encoding Streptomyces griseus protease D (SGPD), a chymotrypsin-like protease, was disrupted in Streptomyces griseus by insertion of the neomysin-resistance gene. The production of chymotrypsin activity of sprD disruptant was not completely abolished, but delayed by 24 h, compared with that of wild-type strain. The aerial mycelial formation of sprD disruptant was retarded, and specifically the formation of spores was not observed in the central region of colonies. However, normal morphological development into spores was observed in the marginal region of colonies. In addition, the production of yellow pigment that might be dependent on A-factor was also decreased in the sprD disruptant, compared with that of the wild-type strain. Introduction of the sprD gene, which was placed on a high copy-numbered plasmid into S. griseus ${\Delta}sprD$, partially restored the ability of morphological development, and a significant level of sporulation was observed. When the overexpression vector for sprD, pWHM3-D, was introduced in S. griseus, there was no significant change in the chymotrypsin activity or colonial morphology, in contrast to Streptomyces lividans, indicating the presence of a tight regulation system for the overexpression of the sprD gene in S. griseus.

• 센서학회지
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• 제25권2호
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• pp.143-147
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• 2016

Characteristics of the conventional gold (Au) surface plasmon resonance (SPR) chip, bi-metallic(Au/silver (Ag)) SPR chip, and tri-metallic(Au/Ag/Au) SPR chip were investigated and compared in the intensity measurement mode for the enhancement of SPR image sensor reactivity. Reflectance curves of the Au, bi- and tri-metallic SPR chips were acquired in phosphate-buffered saline (PBS) solution and were compared. The line width of the reflectance curve of the bi-metallic chip was the narrowest among the three different types of the chips. Also, the tangential slope of the bi-metallic chip was steeper than those of the other chips. Various concentrations of bovine serum albumin (BSA) were utilized in the SPR experiment. As a result, among the above three chips reflectance variation value of the bi-metallic chip was the largest.

• Safety and Health at Work
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• 제14권3호
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• pp.267-271
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• 2023

Background: The objective of this study is to identify the working conditions and health status of Vietnamese male migrant workers in Republic of Korea, in comparison to the Korean general population. Methods: We conducted our survey through the Migrant People Center, and we received completed questionnaires from 87 male Vietnamese migrant workers. The questionnaire employed was identical to those used in the Korean Working Conditions Survey and the 2020 Korea National Health and Nutrition Examination Survey. The collected data from the Vietnamese migrant workers was then compared with the Korean reference population using indirect age-standardization. Results: Vietnamese male workers demonstrated a higher prevalence of health problems including hearing problems (age-standardized prevalence ratio (aSPR) 13.22, 95% confidence interval [CI]: 8.07-20.4), skin problems (aSPR 13.49, 95% CI: 8.07-20.4), and low back pain (aSPR 8.40, 95% CI: 6.50-10.69). Elevated exposure to workplace hazards such as chemicals (aSPR 2.36, 95% CI: 1.51-3.51), organic solvents (aSPR 2.22, 95% CI: 1.44-3.28), handling of heavy objects (aSPR 1.67, 95% CI: 1.24-2.21), and high temperatures (aSPR 1.96, 95% CI: 1.46-2.57) was observed among them. Additionally, they faced a higher risk of no personal protective equipment (aSPR 2.53, 95% CI: 1.26-4.52) and a greater prevalence of unmet medical needs (aSPR 7.14, 95% CI: 4.74-10.32). Conclusion: Our findings highlight the elevated workplace hazards, health problems, and unmet medical needs among Vietnamese male workers compared to the Korean reference population. These findings underscores the urgency for enhanced scrutiny over working conditions and protective equipment provision, coupled with efforts to improve healthcare accessibility and worker education.

• 한국기계가공학회지
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• 제19권9호
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• pp.33-39
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• 2020

Self-piercing rivets are often used in the automotive industry, among other industries, as mechanical components to join multiple materials such as aluminum alloys. Self-piercing rivets have a strong sealing property, although there is considerable scope for their performance improvement. In this study, to enhance the performance of self-piercing rivets, the hybrid self-piercing riveting (SPR) technique, using the existing SPR and structural adhesive, was proposed. Moreover, heterogeneous material specimens subjected to the hybrid SPR technique were manufactured and tested. The joint strength of the test pieces of different materials was evaluated through finite element analyses.

• 센서학회지
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• 제17권5호
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• pp.369-374
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• 2008

A novel fiber optic surface plasmon resonance (SPR) sensor using cyclic olefin copolymer (COC) prism with the spectral modulation is presented. The SPR sensor chip is fabricated using the SU-8 photolithography, Ni-electroplating and COC injection molding process. The sidewall of the COC prism is partially deposited with Au/Cr (45/2.nm thickness) by e-beam evaporator, and the thermal bonding process is conducted for micro fluidic channels and optical fibers alignment. The SPR spectrum for a phosphate buffered saline (0.1.M PBS, pH.7.2) solution shows a distinctive dip at 1300.nm wavelength, which shifts toward longer wavelength with respect to the bovine serum albumin (BSA)concentrations. The sensitivity of the wavelength shift is $1.16\;nm{\cdot}{\mu}g^{-1}{\cdot}{\mu}l^{-1}$. From the wavelength of SPR dips, the refractive indices (RI) of the BSA solutions can be theoretically calculated using Kretchmann configuration, and the change rate of the RI was found to be $2.3{\times}10^{-5}RI{\cdot}{\mu}g^{-1}{\cdot}l^{-1}$. The realized fiber optic SPR sensor with a COC prism has clearly shown the feasibility of a new disposable, low cost and miniaturized SPR biosensor for biochemical molecular analyses.

• Food Science and Biotechnology
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• 제17권3호
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• pp.547-552
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• 2008

The detection of carbamate (carbofuran, carbaryl, benfracarb, thiodicarb, and methomil) and organophosphate (diazinon, cadusafos, ethoprofos, parathion-methyl, and chlorpyrifos) pesticide residues with very low detection limits was carried out using surface plasmon resonance (SPR) based equipment. The capacity to develop a portable SPR biosensor for food safety was also investigated. The applied ligand for the immunoassays was polyclonal goat anti-rabbit immunoglobulin (IgG) peroxidase conjugate. Concentration tests using direct binding assays showed the possibility of quantitative analysis. For ligand fishing to find a proper antibody to respond to each pesticide, acetylcholinesterase (AChE), and glutathione-S-transferase (GST) were tested. The reproducibility and precision of SPR measurements were evaluated. With this approach, the limit of detection for pesticide residues was 1 ng/mL and analysis took less than 11 min. Thus, it was demonstrated that detecting multi-class pesticide residues using SPR and IgG antibodies provides enough sensitivity and speed for use in portable SPR biosensors.