• Molecules and Cells
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• v.27 no.1
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• pp.99-103
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• 2009

Ribose-5-phosphate isomerase A (RpiA) plays an important role in interconverting between ribose-5-phosphate (R5P) and ribulose-5-phosphate in the pentose phosphate pathway and the Calvin cycle. We have determined the crystal structures of the open form RpiA from Vibrio vulnificus YJ106 (VvRpiA) in complex with the R5P and the closed form with arabinose-5-phosphate (A5P) in parallel with the apo VvRpiA at $2.0{\AA}$ resolution. VvRpiA is highly similar to Escherichia coli RpiA, and the VvRpiA-R5P complex strongly resembles the E. coli RpiA-A5P complex. Interestingly, unlike the E. coli RpiA-A5P complex, the position of A5P in the VvRpiA-A5P complex reveals a different position than the R5P binding mode. VvRpiA-A5P has a sugar ring inside the binding pocket and a phosphate group outside the binding pocket: By contrast, the sugar ring of A5P interacts with the Asp4, Lys7, Ser30, Asp118, and Lys121 residues; the phosphate group of A5P interacts with two water molecules, W51 and W82.

• Biomedical Science Letters
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• v.2 no.1
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• pp.109-114
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• 1996

A study was conducted to evaluate isolation rate and distribution of mating type of M.gypseum complex Arthroderma(A.) gypsea and A.incurvata. Seventeen(17) strains were isolated from children patients and sixty(60) strains were from elementary school playgrounds, flower beds, and childrens playgrounds in Taegu area. The strains were crossed with test strains of A.gypsea and A. incurvata which were provided by Tokyo university. The results were summarized as follows; Of seventeen(17) strains, thirteen(13) were isolated from male children and ten(10) were A.incurvata and three(3) A.gypsea, and four(4) were from female children and all iou, strains were A.incurvata. Face(Tinea facial) was more frequently affected to compare with other clinical type. Of seventeen(17) strains isolated from patients, six(6) were A.incurvata "+" type and eight (8) were "-" type, and two(2) were A.gypsea "+" type and one(1) was "-" type. Of sixty (60) strains isolated from soil, sixteen (16) were A.gypsea "+" type and fifteen(15) were"-" type. and fifteen(15) were A. incurvata "+" type and fourteen(14) were "-" type.) were A. incurvata "+" type and fourteen(14) were "-" type. &"-&" type.t; type.

• Korean Journal of Microbiology
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• v.38 no.1
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• pp.38-44
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• 2002

Pneumococcal surfacce protein A (PspA) is an important virulence factor and an antigenically variable surface protein of the pneumococci. To purify the PspA from S. pneumoniae KNIH1156 , a clinical isolate (type 19F), we have taken advantage of the fact that PspA is released from the surface of pneumococci into the medium by growing in a CDM-ET medium and PspA is capable of binding human lactoferrin, the iron carrier protein. PspA of S. pneumoniae KNIH1156 was purified from culture supernatant by human lactoferrin (hLf) affinity chromatography. The purified PspA was confirmed with anti-PspA antiserum and also had the binding capacity to hLf specifically. To determine whether the purified PspA could elicit protection in mice against pneumococcal inflection, we immunized the mice with purified PspA and subsequently challenged with S. pneumoniae KNIH1156. Immunization with purified PspA protected mice from 500 times the $LD^{50}$ of S. pneumoniae KNIH1156. Therefore, it has been shown that purified PspA fromS. pneumoniae KNIH1156 (type 19F) is a protective immunogen.

• 한국균학회소식:학술대회논문집
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• 2015.11a
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• pp.28-28
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• 2015

Asexual reproduction or conidiation in aspergilli is a primary mean to produce their progenies that is environmentally and genetically controlled tightly. Previously, intensive researches in the model fungus Aspergillus nidulans disclosed some genes playing important roles in asexual and sexual development. Among them, one gene encoding a putative helix-loop-helix (HLH) transcription factor, named ndrA, has been isolated and characterized as a downstream regulator of developmental master regulator NsdD. By using comparative genome search of A. niduans NdrA protein, its orthologues have been identified in A. fumigatus and A. flavus, respectively (AfudrnA and AfldrnA). Deletion of the ndrA genes in both Aspergillus species made them unable to produce the conidia yet abundant production of sclerotia in A. flavus. Complementation of ndrA deletion strains by intact ndrA ORFs has restored the conidiation as in the control strains. In A. fumigatus, ndrA deletion also resulted in loss of conidiation phenotype. Northern analyses showed that the ndrA genes in both Aspergillus species are highly expressed at the early stage of the conidiation. Interestingly, the ndrA genes were found to be necessary for the proper expression of brlA genes. Antifungal sensitivity test revealed that the ndrA genes might be responsible for the sensitivity or resistance to some antifungal agents. However, ndrA deletion did not greatly influence the growth in both strains. And the A. flavus ndrA gene did not affect the aflatoxin production. Taken together, ndrA genes in Aspergillus species could be an important positive regulator of conidiation under the regulation of the nsdD gene yet upstream of the brlA gene.

• Membrane Journal
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• v.25 no.2
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• pp.107-114
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• 2015

PTMSP-NaA zeolite composite membranes were prepared by adding 0~50 wt% NaA zeolite to PTMSP. The membranes were characterized by FT-IR, $^1H$-NMR, GPC, DSC, TGA, SEM. The permeabilities of $H_2$ and $N_2$ gases through PTMSP-NaA zeolite composite membranes was studied as a function of NaA zeolite contents. According to TGA measurements, when zeolite was inserted into the polymer, weight loss temperature and weight loss wt% of PTMSP-NaA zeolite composite membranes were decreased. Based on SEM observation, NaA zeolite was dispersed in the PTMSP-NaA zeolite composite membrane with the size $2{\sim}5{\mu}m$. The permeability of PTMSP-NaA zeolite composite membranes increased added as NaA zeolite content increased. On the contrary, the selectivity ($H_2/N_2$) of the composite membranes decreased as NaA zeolite content increased. PTMSP-NaA zeolite composite membrane showed better permeability and selectivity ($H_2/N_2$) of $H_2$ and $N_2$ than PTMSP-NaY zeolite composite membrane.

• The Journal of Churna Manual Medicine for Spine and Nerves
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• v.12 no.1
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• pp.71-81
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• 2017

Objectives : It is important to develop a new Chuna manual therapy as a representative of Chuna manual therapy technique at the time of KSCMM's (Korean Society of Chuna manual medicine) joining a $F{\acute{e}}d{\acute{e}}ration$ Internationale de $M{\acute{e}}decine$ Manuelle (FIMM). Therefore, Sunu manual therapy (SMT) will be introduced as a purely new Chuna manual therapy. Main subject : Sunu manual therapy (SMT) was discovered about two decade ago by a Korean Medicine doctor who wanted to develop a new manual therapy to treat a internal disease. It is very important to make the best use of four powers such as a compression force, a torsion force, a bending force, a tension force using a resonance principle and to experience a feeling of spine axis. SMT is different from Chiropractic technique and Osteopathy technique, in that SMT could treat so many disease using only one technique, but other manual therapy could utilize so many technique to treat only one disease. Discussion : Because SMT utilize a power of a moving in silience, it will take several years to totally master a SMT which will make a patient and a doctor experience a feeling of spine axis. As a feeling of spine axis is deeper, a nerve could be excited and treated by a SMT. Also as a feeling of spine axis is shallower, a muscle could be excied and treated by a SMT. Conclusions : SMT could be classed as a craniosacral chuna therapy or visceral chuna therapy to treat a internal disease considering SMT's action and purpose.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.48-51
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• 2000

The poly(3-hydroxybutyrate) (PHB) synthase of Ralstonia. eutropha, which was produced by a recombinant strain E. coli and purified in one-step with a methyl-HIC column to a purity of more than 90%, was used to polymerize 3-hydroxypropionyl-CoA (3HPCoA) and to copolymerize 3HPCoA with 3-hydroxybutyryl-CoA (3HBCoA) in vitro. A $K_m$ of $189\;{\mu}M$ and a $k_{cat}$ of $10\;sec^{-1}$ were determined for the activity of the enzyme in the polymerization reaction of 3HPCoA based on the assumption that the dimer form of PHB synthase was the active form. Free coenzyme A was found to be a very effective competitive inhibitor for the polymerization of 3HPCoA with a $K_i$ of $85\;{\mu}M$. The maximum degree of conversion of 3HPCoA to polymer was less than 40 %. In the simultaneous copolymerization reactions of these two monomers, both the turnover number for the copolymerization reaction and the maximum degree of conversion of 3HPCoA and 3HBCoA to copolymers increased with an increase in the amount of 3HBCoA in the monomer mixture. However, the maximum conversion of 3HPCoA to a copolymer was less than 35 % regardless of the ratio of 3HPCoA to 3HBCoA. Block copolymers were obtained by the sequential copolymerization of the two monomers and these copolymers had a much narrower molecular weight distribution than those obtained by the simultaneous copolymerization of the same molar ratio of 3HPCoA and 3HBCoA.

• Journal of Fashion Business
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• v.9 no.6
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• pp.141-159
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• 2005

Targeting a film that is the medium of having powerful influence upon the masses, the present study examined about a role and characteristics in the movie costume, and a role of costume designers, which are shown in process of being changed the film costume. There are many designers who were in charge of the film costume, but the present study examined centering on designers who participated aiming to create the image of a character from the stage of manufacturing a movie. It presented and analyzed visual materials by dividing four genres such as a historical drama movie, a horror movie, a fantasy movie, and a modern-play movie, and by selecting a typical work. A Historical drama movie needs to be investigated costume by the historical background in a movie, but inside it was shown clothes that were elaborately reproduced and newly created. A horror movie plays a role of medium that reflects the human society and the internal mentality of a human being along with the attribute of entertainment. As a genre that requires much costume, make-up and special effect aiming at dramatic effect, a role of film costume possesses great weight. As a fantasy movie is a field based on 'fiction' of a writer who creates a work, it is a field that requires creativity of a costume designer most. As a modern-play movie is what reproduces reality, it best reflects the phases of that time, and is the field that is influenced by costume or fashion trend. Costume needs to be designed in a bid to allow spectators to be inspired the wholly united and harmonious mood with leading a story of a movie, and the individual image.

• Journal of Korean Medical classics
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• v.19 no.3
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• pp.165-183
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• 2006

A study of scientific methods in a study of comparative on the oriental medicine and the western medicine evidence to find on the cause-effect relationship. The rationality in a study of process and the confidence in a study of result were improved by a study of methods on the cause-effect relationship. Also a comparative study on the Jeong(精) of the oriental medicine and the cell of the western medicine, after established a model of the cause-effect relationship secured a suitability of a comparative subject and operationalization of a comparative variable to the rationality in a study of process. The Jeong(精) and the cell are the character of a unit and the character of a matter on the human body. The Jeong(精) and the cell are a point of similarity in the function. The Jeong(精) and the cell are a relation in the mechanism. Our paper reviewed a study of comparative methods on the oriental medicine and the western medicine, and suggest an identic interpretation on the human body.

• Journal of Fisheries and Marine Sciences Education
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• v.21 no.2
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• pp.247-258
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• 2009

Although the problem about a token of parents' gratitude is restricted by small number of teachers, it must be solved for normal and good schooling. The purpose of this study was to investigate the problem about a token of parents' gratitude, an extent of a token of parents' gratitude, seriousness of a token of parents' gratitude, the experience of a token of parents' gratitude, an amount of money a token of parents' gratitude, the time of a token of parents' gratitude, a compensation of a token of parents' gratitude using a questionnaire to 549 parents. And a Chi-square test was used as a research tool for this study. This study indicates that many parents recognize a token of parents' gratitude as money or gift certificate. And most parents recognize different significantly about a token of parents' gratitude according to teachers' sex, ages, level of school, and type of school. We do not need a vast and experimental revolution in education based on unproved reasoning. If we want to be normal and good schooling, we have to make an effort small changes which solve a problem of a token of parents' gratitude. Our educational policies have to move from vast approaches generated from 'above' with political purpose to more particular approaches based on specific problems and generated by the community.