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Blood Chemistry Profiles in Indigenous Korean Calves According to Age (한우 송아지의 성장에 따른 혈액화학치에 관한 연구)

  • Lee, Soo-Doo;Cho, Kwang-Hyun;Eo, Kyung-Yeon;Kwak, Dongmi;Kwon, Oh-Deog
    • Journal of Veterinary Clinics
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    • v.32 no.4
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    • pp.392-397
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    • 2015
  • Blood chemistry values according to ages have rarely been investigated in indigenous Korean calves. The present study aimed to clarify the changes in blood chemistry values of indigenous Korean calves with age. Blood samples were collected from 29 calves (11 females and 18 males) reared at the Gyeongsangbukdo Livestock Research Institute and the levels of various chemical components in the serum were analyzed. The total protein, albumin and globulin concentration in the calves increased gradually over time. The glucose concentration increased significantly at 2 weeks after birth, and then decreased slightly 4 weeks after birth. The blood urea nitrogen concentration increased from 2 weeks after birth, and the levels in females were higher than those in males at 2 and 4 weeks after birth. The creatinine concentration decreased significantly from 2 weeks after birth, and the levels in females were higher than those in males at 2 weeks and 6 months after birth. The total bilirubin concentration decreased gradually from 2 weeks after birth. The glutamic oxaloacetic transaminase and glutamic pyruvic transaminase concentration increased gradually from 2 weeks after birth. The triglyceride concentration tended to increase from 2 weeks after birth. The total cholesterol concentration increased significantly at 2 weeks after birth, and then decreased slightly 4 weeks after birth. The total high density lipoprotein cholesterol concentration increased significantly from 2 weeks after birth. The calcium concentration was maintained between $9.8{\pm}0.8mg/dL$ and $11.6{\pm}0.9mg/dL$ throughout the investigation period, although some levels fluctuated at 4 weeks and 6 months after birth. The phosphorus concentration increased significantly at 2 weeks after birth, and then decreased slightly 4 weeks after birth. In conclusion, the data obtained from this study may be valuable for use as a standard for interpreting results of blood biochemical analyses in indigenous Korean calves.

Genotype of Group A Rotavirus Isolated in Acute Gastroenteritis Patients and Groundwater in Seoul, Korea (서울지역 급성위장관염 환자 및 지하수에서 분리한 A형 로타바이러스의 유전자형)

  • Kim, Eun-Jeung;Kim, Moo-Sang;Chae, Young-Zoo;Cheon, Doo-Sung
    • Korean Journal of Microbiology
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    • v.47 no.4
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    • pp.323-327
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    • 2011
  • Fecal specimens from acute gastroenteritis in Seoul from 2009 to 2010 were collected and then tested for the presence of Group A Rotavirus by ELISA. Among a total of 1,916 samples investigated, 354 samples (18.4%) were positive. The predominant genotypes of positive samples were confirmed as P6G[4] (35%), P8G[3] (28%), P8G[1] (24%), P4G[2] (10%), P8G[9] (3%), respectively. Among a total of 70 ground water samples investigated, 2 samples (2.8%) were positive. The genotypes of positive samples were confirmed as P8G[3] (100%). By this molecular investigation, genotypic distribution associated with rotavirus will be used for control and prevention of rotavirus related diseases.

Effect of Chrysanthemum Morifolium Extracts on the Synthesis of Laminin of Madin-Darby Canine Kidney Cells (감국(甘菊)이 MDCK 세포의 Laminin 합성에 미치는 영향)

  • Na, Ho-Jeong;Jeon, So-Ra;Cha, Dong-Seok;Eun, Jae-Soon;Lim, Jong-Pil;Shin, Tae-Yong;Oh, Chan-Ho;Yang, Jae-Heon;Kim, Dae-Keun;Leem, Jae-Yoon;Chae, Byeong-Suk;Kim, Sung-Zoo;Jung, Yen-Ok;Jeong, Won-Hwan;Jeon, Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.3
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    • pp.709-713
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    • 2007
  • Basement membranes (BMs) are extracellular matrices associated with epithelia, endothelia, muscle, fat and peripheral nerve. They are involved in cell survival, migration, differentiation. BMs functions also include tissue formation and provide mechanical stability as a selective barriers. Laminins are heterotrimeric glycoproteins found in BMs and have a crucial role in cell adhesion and signalling. Madin-Darby canine kidney (MDCK) cells are the best established mammalian model for studying epithelial cell biology The cells form an epithelial monolayer, with tight junctions separating an apical surface from a basolateral membrane facing the filter support and neighboring cells. In this study, using MDCK cells, the synthesis of the BM protein such as laminin with or without methanol extract of Chrysanthemum morifolium (CM) stimulation was analyzed by immunoblotting and CM showed significant increased cell density and enhanced synthesis of laminin.

Arthrostoma miyazakiense (Nematoda: Ancylostomatidae) infection in raccoon dogs of Korea and experimental transmission to dogs

  • Shin, Sung-Shik;Cha, Dae-Jung;Cho, Kyoung-Oh;Cho, Ho-Sung;Choi, Jeong-Ok;Cho, Shin-Hyeong
    • Parasites, Hosts and Diseases
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    • v.45 no.2 s.142
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    • pp.121-128
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    • 2007
  • Arthrostoma miyazakiense (Nematoda: Ancylostomatidae) is a hookworm species reported from the small intestines of raccoon dogs (Nyctereutes procyonoides) in Japan. Five Korean raccoon dogs (N. procyonoides koreensis) caught from 2002 to 2005 in Jeollanam-do (Province), a southeastern area of South Korea, contained helminth eggs belonging to 4 genera (roundworm, hookworm, whipworm, and Capillaria spp.) and cysts of Giardia sp. in their feces. Necropsy findings of 1 raccoon dog revealed a large number of adult hookworms in the duodenum. These hookworms were identified as Arthrostoma miyazakiense based on the 10 articulated plates observed in the buccal capsule and the presence of right-sided prevulval papillae. Eggs of A. miyazakiense were $60-65{\times}35-40{\mu}m$ (av, $62.5{\times}35{\mu}m$), and were morphologically indistinguishable from those of Ancyiostoma caninum. The eggs were cultured to infective 2nd stage larvae via charcoal culture, and 100 infective larvae were used to experimentally infect each of 3 mixed-bred puppies. All puppies harbored hookworm eggs in their feces on the 12th day after infection. This is the first report thus far concerning A. miyazakiense infections in raccoon dogs in Korea, and the first such report outside of Japan.

Determining Method of Sulfites in Foods by Ion Chromatography

  • Kim, Myung-Hee;Chae, Young-Zoo
    • Journal of Food Hygiene and Safety
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    • v.3 no.4
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    • pp.211-215
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    • 1988
  • Anion chromatographic (lC) method was developed for the determination of sulfites infoods. Sulfites refer to sulfur dioxide that was separated from a food sample by addition of acid and heating, and carried into a trapping solution by distillation. The trapping solution was applied to IC system. Sulfites was separated on an anionic separator column, HPIC-AS4A with 0.75mM $NAHCO_3/2.25mM\;Na_2CO_3$ as the eluent and determined by the use of conductivity detector. The recoveries of sulfites added to water, carrot and apple at level of 1 ppm were 99.8%, 91.6% and 83.5%, respectively. The detection limit was 0.2 ppm in the case of a 10 g sample size. All experiment could be finished within 20 minutes

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Diamond-Like Carbon Films Deposited by Pulsed Magnetron Sputtering System with Rotating Cathode

  • Chun, Hui-Gon;You, Yong-Zoo;Nikolay S. Sochugov;Sergey V. Rabotkin
    • Journal of Surface Science and Engineering
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    • v.36 no.4
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    • pp.296-300
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    • 2003
  • Extended cylindrical magnetron sputtering system with rotating 600-mm long and 90-mm diameter graphite cathode and pulsed power supply voltage generator were developed and fabricated. Time-dependent Langmuir probe characteristics as well as carbon films thickness were measured. It was shown that ratio of ions flux to carbon atoms flux for pulsed magnetron discharge mode was equal to $\Phi_{i}$ $\Phi$sub C/ = 0.2. It did not depend on the discharge current in the range of $I_{d}$ / = 10∼60 A since both the plasma density and the film deposition rate were found approximately proportional to the discharge current. In spite of this fact carbon film structure was found to be strongly dependent on the discharge current. Grain size increased from 100 nm at $I_{d}$ = 10∼20 A to 500 nm at $I_{d}$ = 40∼60 A. To deposit fine-grained hard nanocrystalline or amorphous carbon coating current regime with $I_{d}$ = 20 A was chosen. Pulsed negative bias voltage ($\tau$= 40 ${\mu}\textrm{s}$, $U_{b}$ = 0∼10 ㎸) synchronized with magnetron discharge pulses was applied to a substrate and voltage of $U_{b}$ = 3.4 ㎸ was shown to be optimum for a hard carbon film deposition. Lower voltages were not sufficient for amorphization of a growing graphite film, while higher voltages led to excessive ion bombardment and effects of recrystalization and graphitization.

Characteristics of NbN Films Deposited on AISI 304 Using Inductively Coupled Plasma Assisted DC Magnetron Sputtering Method

  • Jun, Shinhee;Kim, Junho;Kim, Sunkwang;You, Yong Zoo;Cha, Byungchul
    • Journal of Surface Science and Engineering
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    • v.46 no.5
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    • pp.187-191
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    • 2013
  • Niobium nitride (NbN) films were deposited on AISI 304 stainless steels by inductively coupled plasma (ICP) assisted dc magnetron sputtering method at different ICP powers, and the effects of ICP power on the phase formation, mechanical and chemical properties of the films were investigated. X-ray diffraction analysis (XRD) and field emission scanning electron microscopy (FESEM) were used to analyze the crystal structure and micro-knoop hardness was used to measure the hardness of the films. Also, 3-D mechanical profiler and a ball-on-disk wear tester were used to measure the thickness of the films and to estimate wear characteristics, respectively. The thickness of the films decreased but their hardness increased with increasing ICP power, and it was confirmed that only cubic ${\delta}$-NbN(200) remained at high ICP power. At lower ICP powers, a mixture of the hexagonal ${\delta}^{\prime}$-NbN and cubic ${\delta}$-NbN phases was obtained in the films and the hardness decreased. The corrosion potential value increased gradually with increasing ICP power, but the changes of ICP power did not significantly influence the overall corrosion resistance.

The Development and Application of Intelligent Welding Carriage with High Deposition Rate by 3-D Weaving (3차원 위빙 대용착 지능 용접캐리지 개발 및 적용)

  • Kim, Young-Zoo;Cho, Bang-Hyun;Amit, Amit;Lee, Sang-Bum;Lee, Weon-Gu;Kim, Jin-Yong;Huh, Man-Joo
    • Journal of Welding and Joining
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    • v.28 no.2
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    • pp.32-38
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    • 2010
  • In shipbuilding industry, welding position are usually flat and vertical position at the erection stage. Application of SAW and EGW for these positions makes it possible to achieve enhanced productivity and high quality. But owing to their large size and weight it is difficult to apply these techniques in short and narrow regions. To overcome this problem, our company developed light weight and compact size 4-axis welding carriage which perform 3D weaving. The purpose of this study is to explain the development and application of intelligent welding carriage using 3D weaving pattern that can fill a large amount of welds and thereby making it possible to achieve high quality of welding. This study shows 3D weaving pattern, development of weaving database, and skill of adaptive control response for the variable gap. Also, it shows the results of procedure qualification test for the AH-grade steel when applied to the intelligent welding carriage.

Effects of environmental enrichment on behaviour, physiology and performance of pigs - A review

  • Mkwanazi, Mbusiseni Vusumuzi;Ncobela, Cypril Ndumiso;Kanengoni, Arnold Tapera;Chimonyo, Michael
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.1
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    • pp.1-13
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    • 2019
  • This paper aims to critically analyse and synthesise existing knowledge concerning the use of environmental enrichment and its effect on behavior, physiology and performance of pigs housed in intensive production systems. The objective is also to provide clarity as to what constitutes successful enrichment and recommend when and how enrichment should be used. Environmental enrichment is usually understood as an attempt to improve animal welfare and to a lesser extent, performance. Common enrichment objects used are straw bedding, suspended ropes and wood shavings, toys, rubber tubings, colored plastic keys, table tennis balls, chains and strings. These substrates need to be chewable, deformable, destructible and ingestible. For enrichment to be successful four goals are essential. Firstly, enrichment should increase the number and range of normal behaviors; secondly, it should prevent the phenomenon of anomalous behaviors or reduce their frequency; thirdly, it should increase positive use of the environment such as space and fourthly it should increase the ability of the animals to deal with behavioral and physiological challenges. The performance, behavior and physiology of pigs in enriched environments is similar or in some cases slightly better when compared with barren environments. In studies where there was no improvement, it should be borne in mind that enriching the environment may not always be practical and yield positive results due to factors such as type of enrichment substrates, duration of provision and type of enrichment used. The review also identifies possible areas that still need further research, especially in understanding the role of enrichment, novelty, breed differences and other enrichment alternatives.

Epidemiological Investigation of Tick Species from Near Domestic Animal Farms and Cattle, Goat, and Wild Boar in Korea

  • Chae, Jeong-Byoung;Cho, Young-Sun;Cho, Yoon-Kyoung;Kang, Jun-Gu;Shin, Nam-Shik;Chae, Joon-Seok
    • Parasites, Hosts and Diseases
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    • v.57 no.3
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    • pp.319-324
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    • 2019
  • This study aimed to investigate the tick species and give background for tick-borne investigations in Korea. Ticks were collected from the area within 2 km radius of the 4 domestic animal farms, where they were located in mountainous areas and raising animals on pasture, and from animal bodies in 2014 and 2015. In total, 7,973 nymphal and adult ticks were collected from the farms - 7,758 Haemaphysalis longicornis, 198 Haemaphysalis flava, and 17 Ixodes nipponensis, and 1,763 were collected from animals - 729 H. longicornis from cattle; 569 H. longicornis from goats; and 297 H. longicornis, 118 H. flava, 1 I. nipponensis, and 49 Amblyomma testudinarium from wild boars. As more species of ticks were collected from wild boars than domesticated animals and their habitats, various animal hosts should be considered while investigating tick species.