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Effects of Silkworm (Bombyx mori L.) Powder on Oxygen Radicals and Their Scavenger Enzymes in Liver of SD Rats (간장조직의 활성산소 및 그 제거효소에 미치는 누에(Bombyx mori L.) 분말의 영향)

  • 최진호;김대익;박수현;김정민;조원기;이희삼;류강선
    • Journal of Life Science
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    • v.10 no.4
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    • pp.347-353
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    • 2000
  • This study was designed to investigate the effects of silkworm (Bombyx mori L.) powder (SWP) on oxygen radicals and their scavenger enzymes in liver membranes of rats. Sprague-Dawley(SD) male rats(160$\pm$10g) were fed basic diet (control group), and experimental diets(SWP-200 and SWP-400 groups) added 200 and 400 mg/kg BW/day for 6 weeks. Hydroxyl radical (.OH) levels resulted in a consistent decreases (4.0% and 7.2%, 5.0% and 14.1%, respectively) in liver mitochondria and microsomes of SWP-200 SWP-400 groups compared with control group, and O2 radical level was significantly decreased about 12% in liver cytosol of SWP-400 group compared with control group. Lipid peroxide(LPO) levels were significantly decreased (14.4% and 9.1%, respectively) in liver mitochondria and microsomes of SWP-400 group only compared with control group. Oxidized protein (OP) levels were remarkably decreased about 12.7% and 16.3% in liver microsomes only of SWP-200 and SWP-400 groups, but significant difference between liver mitochondria could not obtained. Mn-SOD activities were remarkably increased (15.8% and 25.2%, respectively) in mitochondria of SWP-200 and SWP-400 groups, but significant difference between Cu, Zn-SOD activities in these group could be not obtained. GSHPx activity was significantly increased in liver cytosol of SWP-400 group compared with control group. These results suggest that silkworm powder may play an effective role in a attenuating a oxidative stress and increasing a scavenger enzyme activity in liver membranes.

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Agricultural Methods for Toxicity Alleviation in Metal Contaminated Soils: A Review

  • Arunakumara, Kkiu;Walpola, Buddhi Charana;Yoon, Min-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.2
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    • pp.73-80
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    • 2013
  • Due to the fact that possible risk associated with soil-crop-food chain transfer, metal contamination in croplands has become a major topic of wide concern. Accumulation of toxic metals in edible parts of crops grown in contaminated soils has been reported from number of crops including rice, soybean, wheat, maize, and vegetables. Therefore, in order to ensure food safety, measures are needed to be taken in mitigating metal pollution and subsequent uptake by crop plants. Present paper critically reviewed some of the cost effective remediation techniques used in minimizing metal uptake by crops grown in contaminated soils. Liming with different materials such as limestone ($CaCO_3$), burnt lime (CaO), slaked lime [$Ca(OH)_2$], dolomite [$CaMg(CO_3)_2$], and slag ($CaSiO_3$) has been widely used because they could elevate soil pH rendering metals less-bioavailable for plant uptake. Zn fertilization, use of organic amendments, crop rotation and water management are among the other techniques successfully employed in reducing metal uptake by crop plants. However, irrespectively the mitigating measure used, heterogeneous accumulation of metals in different crop species is often reported. The inconsistency might be attributed to the genetic makeup of the crops for selective uptake, their morphological characteristics, position of edible parts on the plants in respect of their distance from roots, crop management practices, the season and to the soil characteristics. However, a sound conclusion in this regard can only be made when more scientific evidence is available on case-specific researches, in particular from long-term field trials which included risks and benefits analysis also for various remediation practices.

CIGS 박막 태양전지를 위한 $(In,Ga)_2Se_3$ 전구체 제작 및 분석

  • Jo, Dae-Hyeong;Jeong, Yong-Deok;Park, Rae-Man;Han, Won-Seok;Lee, Gyu-Seok;O, Su-Yeong;Kim, Je-Ha
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.285-285
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    • 2010
  • $Cu(In,Ga)Se_2$ (CIGS) 박막 태양전지 제조에는 동시증발법 (co-evaporation)으로 Cu, In, Ga, Se 각 원소의 증발을 세 단계로 제어하여 CIGS 박막을 증착하는 3-stage 방법이 널리 이용된다[1]. 3-stage 중 1st-stage에서는 In, Ga, Se 원소 만을 증발시켜 $(In,Ga)_2Se_3$ 전구체 (precursor) 박막을 성장시킨다. 고효율의 CIGS 태양전지를 위해서는 $(In,Ga)_2Se_3$ 전구체 증착의 공정 변수와 이에 따른 박막 특성의 이해가 중요하다. 본 연구에서는 Mo 박막이 증착된 소다석회유리 (soda lime glass) 기판에 동시증발장비를 이용하여 280 380 의 기판 온도에서 In, Ga, Se 물질을 증발시켜 $(In,Ga)_2Se_3$/Mo/glass 시료를 제작하였으며 XRD, SEM, EDS 등의 방법을 이용하여 특성을 분석하였다. XRD 분석 결과 기판 온도 $280{\sim}330^{\circ}C$에서는 $(In,Ga)_2Se_3$ 박막의 (006), (300) 피크가 관찰되었으며, 기판 온도가 증가할수록 (006) 피크 세기는 감소하였고 (300) 피크 세기는 증가하였다. $380^{\circ}C$에서는 (110)을 포함한 다수의 피크가 관찰되었다. 그레인 (grain) 크기는 기판 온도가 증가할수록 커지며 Ga/(In+Ga) 조성비는 기판 온도에 따라 일정함을 각각 SEM과 EDS 측정을 통해 알 수 있었다. $(In,Ga)_2Se_3$ 전구체의 (300) 배향은 CIGS 박막의 (220/204) 배향을 촉진하고[2], 이것은 높은 광전변환효율에 기여하는 것으로 알려져 있다. 때문에 $(In,Ga)_2Se_3$의 (300) 피크의 세기가 가장 큰 조건인 $330^{\circ}C$를 1st-stage 증착 온도로 하여 3-stage CIGS 태양전지 공정을 수행하였으며, $MgF_2$/Al/Ni/ITO/i-ZnO/CdS/CIGS/Mo/glass 구조의 셀에서 광전변환효율 16.96%를 얻었다.

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A Study on the Influence of Water Quality on the Phosphorus Fraction Properties from Reservoir Sediments (저수지 퇴적물로부터 인의 존재형태가 수질에 미치는 영향에 대한 연구)

  • Lee, Jin-Kyung;Ahn, Tae-Woong;Oh, Jong-Min
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.9
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    • pp.840-850
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    • 2010
  • The present study was attempted to find the effects of structural properties of phosphorus on the water quality of Gyehwa reservoir in Saemangeum. Relationship of phosphorus fractions between water and sediment properties was closely examined, and a few types of phosphorus were found from the sample sediment as : Saloid-P, Al-P, Fe-P, Ca-P, Red-P and Occd-P. Saloid-P (1.4%), Al-P (0.5%), Fe-P (39.8%), Ca-P (56.6%), Red-P (0.4%), Occd-P (1.3%) were extracted in a mass basis from the sediment of Gyehwa reservoir. Approximately more than 97% of phosphorus were calcium related phosphorus (Ca-P, 56%) and iron bound phosphorus (Fe-P, 39.8%). The Fe-P closely relates with water quality of T-N (r=0.761, p<0.05), $NO_3$-N (r=0.754, p<0.05), $NH_4$-N (r=0.728, p<0.05), T-P (r=0.774, p<0.05) and $PO_4$-P (r=0.767, p<0.05) while the Ca-P did not show any consistent dependency on the water quality. On the other hand, the correlation of Ca-P with $P_2O_5$ was high with r=0.783 (p<0.05) in the sediment. The Fe-P was affected significantly on the Ignition Loss (r=0.569, p<0.05), T-N (r=0.715, p<0.05) and T-P (r=0.983, p<0.01). In the research of correlation between phosphorus fraction and heavy metals in the sediment, Ca-P did not show any specific relationships with heavy metals. The Fe-P showed a significant correlation with As (r=0.817, p<0.01), Cu (r=0.793, p<0.05), Cd (r=0.786, p<0.05), Zn (r=0.738, p<0.05), so that it can be stated that the presence of Fe-P may implicate the volume of various metallic elements.

A Study on the Evaluation of Fertilizer Loss in the Drainage(Waste) Water of Hydroponic Cultivation, Korea (수경재배 유출 배액(폐양액)의 비료 손실량 평가 연구)

  • Jinkwan Son;Sungwook Yun;Jinkyung Kwon;Jihoon Shin;Donghyeon Kang;Minjung Park;Ryugap Lim
    • Journal of Wetlands Research
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    • v.25 no.1
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    • pp.35-47
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    • 2023
  • Korean facility horticulture and hydroponic cultivation methods increase, requiring the management of waste water generated. In this study, the amount of fertilizer contained in the discharged waste liquid was determined. By evaluating this as a price, it was suggested to reduce water treatment costs and recycle fertilizer components. It was evaluated based on the results of major water quality analysis of waste liquid by crop, such as tomatoes, paprika, cucumbers, and strawberries, and in the case of P component, it was analyzed by converting it to the amount of phosphoric acid (P2O5). The amount of nitrogen (N) can be calculated by discharging 1,145.90kg·ha-1 of tomatoes, 920.43kg·ha-1 of paprika, 804.16kg·ha-1 of cucumbers, 405.83kg·ha-1 of strawberries, and the fertilizer content of P2O5 is 830.65kg·ha-1 of paprika, 622.32kg·ha-1 of tomatoes, 477.67kg·ha-1 of cucumbers. In addition, trace elements such as potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), and manganese (Mn) were also analyzed to be emitted. The price per kg of each item calculated by averaging the price of fertilizer sold on the market can be evaluated as KRW, N 860.7, P 2,378.2, K 2,121.7, Ca 981.2, Mg 1,036.3, Fe 126,076.9, Mn 62,322.1, Zn 15,825.0, Cu 31,362.0, B 4,238.0, Mo 149,041.7. The annual fertilizer loss amount for each crop was calculated by comprehensively considering the price per kg calculated based on the market price of fertilizer, the concentration of waste by crop analyzed earlier, and the average annual emission of hydroponic cultivation. As a result of the analysis, the average of the four hydroponic crops was 5,475,361.1 won in fertilizer ingredients, with tomatoes valued at 6,995,622.3 won, paprika valued at 7,384,923.8 won, cucumbers valued at 5,091,607.9 won, and strawberries valued at 2,429,290.6 won. It was expected that if hydroponic drainage is managed through self-treatment or threshing before discharge rather than by leaking it into a river and treating it as a pollutant, it can be a valuable reusable fertilizer ingredient along with reducing water treatment costs.

Physico-chemical Meat Quality Properties and Nutritional Composition of Hanwoo Steer Beef with 1++ Quality Grade (1++ 등급 거세한우의 부위별 이화학적 육질특성 및 영양성분조성)

  • Cho, S.H.;Kim, J.H.;Seong, P.N.;Cho, Y.M.;Chung, W.T.;Park, B.Y.;Chung, M.O.;Kim, D.H.;Lee, J.M.;Ahn, C.N.
    • Food Science of Animal Resources
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    • v.28 no.4
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    • pp.422-430
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    • 2008
  • This study was conducted to investigate the nutritional composition and meat quality properties of Hanwoo steer beef by different cut. Ten cuts [Abjin (short plate), Bosup (top sirloin), Cheggt (striploin), Dngsim (loin), Guri (chuck tender), Hongduke (eye of round), Moksim (chuck roll), Sulgit (bottom round), Udoon (top round), Yanaji (brisket)] prepared from 10 Hanwoo steers (28-30 months old) were used for this experiment. The range of pH was 5.46-5.64. In the chemical analysis, Hongduke contained highest protein contents (21.15%) and Dngsim had lowest protein contents (16.94%) (p<0.05). Fat contents were higher in Dngsim whereas those of Guri, Hongduke and Udoon were significantly lower when compared to the other cuts (p<0.05). There were not significantly different in meat color (L, a and b values) properties only except that L values were significantly higher in Dngsim among 10 cuts (p<0.05). Cooking loss (%) was higher in Guri, Hongduke, Udoon and Yangji whereas it was significantly love. in Cheggt (p<0.05). Warner-Bratzler shear force (WBS) was significantly higher in Abjin $(6.24inch^2/kg)$, whereas Dngsim $(3.45inch^2/kg)$ and Cheggt $(3.50inch^2/kg)$ were significantly lower than the other cuts (p<0.05). There was no significant difference in water-holding capacity (WHC) among 10 cuts (p>0.05). Total amino acid contents were significantly higher in Hongduke (p<0.05). Hongduke was highest in most kinds of amino acids, however, Udoon had the highest methionine and histidine contents among 10 cuts (p<0.05). Glutamate contents were high in all cuts and followed by aspartate, leucine and lysin. With regard to mineral contents, Ca was ranged from 47.63-70.69 mg/kg and Fe was ranged from 15.09-26.68 mg/kg in 10 cuts. Zn was highly contained in Guri (50.56 mg/kg) when compared to the other cuts (p<0.05).

Fertilizing Effects of Swine Compost Fermented with Sawdust on Mixed Pastures (혼파초지에 대한 톱밥발효돈분의 시용효과)

  • Shin, J. Soon;Cho, Young-Mu;Lee, Hyo-Ho;Yoon, Sea-Hung;Park, Geun-Je;Choi, Ki-Chun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.3
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    • pp.245-252
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    • 2004
  • Experiment was carried out to find the fertilizing effects of 8 different application rates of swine compost fermented with sawdust(SCS) including Chemical fertilizer(CF) on forage yield and soil chemical characteristics of mixed pastures sown in Sep. 1993 at National Livestock Research Institute, RDA., in Suwon during low years. It was arranged in a randomized complete block design with three replicates. Dry matter yield were shown at similar among treatments except Control and $50\%$ SCS of standard amount plot. In botanical composition, the legume and weeds percentages of each treatment were increased as advancing year. The final year's legume percentage were high in line with SCS fertilizing plots($39\%{\sim}43\%$), SCS + CF plots($30\%{\sim}41\%$) and CF plot($32\%$). In productions of TDN, NE and crude protein yield, SCS or SCS($75\%$) + CF($25\%$) were nearly same comparing those of CF, respectively. Phosphate, potassium, magnesium contents and K/(Ca + Mg) except calcium contents of those SCS fertilizing plots in plant were generally high with comparing CF. Those contents were proportional according to the fertilizing amount These result indicate the possibility to substitute chemical fertilizer for SCS($75\%$, 25ton/ha) + CF, $25\%$) as manure-N 210 kg/ha, but might be considered accumulation phosphate in the soil.

Geochemical Characteristics of Intertidal Surface Sediments along the Southwestern Coast of Korea (한국 서해남부 조간대 표층 퇴적물의 지화학적 특성)

  • Hwang, Dong-Woon;Ryu, Sang-Ok;Kim, Seong-Gil;Choi, Ok-In;Kim, Seong-Soo;Koh, Byoung-Seol
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.2
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    • pp.146-158
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    • 2010
  • In order to evaluate the characteristics of sediments and pollution by organic matter and metallic elements in intertidal sediments along the southwestern coast of Korea, we measured various geochemical parameters, including the mean grain size (Mz), water content (WC), ignition loss (IL), chemical oxygen demand (COD), acid volatile sulfide (AVS), and metallic elements (Al, Fe, Cu, Pb, Zn, Cd, Cr, Mn, Hg, As), in intertidal surface sediments. The Mz of the surface sediments ranged from 2.1 to 8.3$\phi$, indicating that the surface sediments consist of various sedimentary facies, such as sand, slightly gravelly mud, sandy mud, and silt. The IL and COD in surface sediment ranged from 0.8 to 5.5% (mean $2.9\pm1.2%$) and from 3.9 to $13.8\;mgO_2/g{\cdot}dry$ (mean $8.5\pm2.6\;mgO_2/g{\cdot}dry$), respectively, which were lower than the values for surface sediment in areas near fish and shellfish farms or industrial complexes. No AVS was detected at any sampling station, despite various sedimentary facies. Most of metallic elements in surface sediments showed relatively good positive correlations with Mz and IL, which imply that the concentrations of metallic elements are mainly controlled by grain size and the organic matter content. The concentrations of metallic elements, except As, at some stations were considerably lower than those in the Sediment Quality Guideline (Effect Range Low, ERL) proposed by the National Oceanic and Atmospheric Administration (NOAA) in the United States. Similarly, the geoaccumulation index (Igeo) class indicated that pollution by metallic elements in intertidal surface sediment, except As, was moderate or non-existent. Our results imply that the intertidal surface sediments along the southwestern coast of Korea are not polluted by organic matter and metallic elements and are healthy for benthic organisms.

Layered structure of sialoliths compared with tonsilloliths and antroliths

  • Buyanbileg Sodnom-Ish;Mi Young Eo;Yun Ju Cho;Mi Hyun Seo;Hyeong-Cheol Yang;Min-Keun Kim;Hoon Myoung;Soung Min Kim
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.50 no.1
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    • pp.13-26
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    • 2024
  • Objectives: The aim of this study was to perform a comparative analysis of the ultrastructural and chemical composition of sialoliths, tonsilloliths, and antroliths and to describe their growth pattern. Materials and Methods: We obtained 19 specimens from 18 patients and classified the specimens into three groups: sialolith (A), tonsillolith (B), and antrolith (C). The peripheral, middle, and core regions of the specimens were examined in detail by histology, micro-computed tomography (micro-CT), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy, and transmission electron microscopy (TEM). Results: In the micro-CT, group A showed alternating radiodense and radiolucent layers, while group B had a homogeneous structure. Group C specimens revealed a compact homogeneous structure. Histopathologically, group A showed a laminated, teardrop-shaped, globular structure. Group B demonstrated degrees of immature calcification of organic and inorganic materials. In group C, the lesion was not encapsulated and showed a homogeneous lamellar bone structure. SEM revealed that group A showed distinct three layers: a peripheral multilayer zone, intermediate compact zone, and the central nidus area; groups B and C did not show these layers. The main elemental components of sialoliths were O, C, Ca, N, Cu, P, Zn, Si, Zr, F, Na, and Mg. In group B, a small amount of Fe was found in the peripheral region. Group C had a shorter component list: Ca, C, O, P, F, N, Si, Na, and Mg. TEM analysis of group A showed globular structures undergoing intra-vesicular calcification. In group B, bacteria were present in the middle layer. In the outer layer of the group C antrolith, an osteoblastic rimming was observed. Conclusion: Sialoliths had distinct three layers: a peripheral multilayer zone, an intermediate compact zone and the central nidus area, while the tonsillolith and antrolith specimens lacked distinct layers and a core.

Chemical Changes of Meju made with Barly Bran Using Fermentation (보리등겨로 제조한 메주의 발효기간에 따른 각종 성분 변화)

  • Kwon, O-Jun;Choi, Ung-Kyu;Lee, Eun-Jeong;Cho, Young-Je;Cha, Won-Senp;Son, Dong-Hwa;Chung, Yung-Gun
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.1135-1141
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    • 2000
  • For investigation of new utilization as jang-products, Meju was prepared using barely bran. As barley meju was fermented, change of pH was $5.2{\sim}5.6$, it was indistinguishable change. L-value of color was changed from 46.9 to 60.3, that meant it was getting moe dark. The counts of aerobic bacteria were $4.8{\times}10^7{\sim}5.6{\times}10^9$ CFU/g, it was extraordinarily increased during fermentation. Counts of Yeast, molds, and bacteria were $9.1{\times}10^6{\sim}5.0{\times}10^8$ CFU/g, $8.3{\times}10^5{\sim}6.9{\times}10^7$, and $2.0{\times}10^2{\sim}4.5{\times}10^6$ CFU/g, respectively. Crude ash content was $3146.0{\sim}7147.4$ mg%. The level of K was the highest in quantity among the crude ash in barely meju. 7 free sugars(i.e., raffnose, stachyose, inositol, fructose, glucose, arabinose, and maltose), 3 volatile organic acid(i.e., acetic acid, propionic acid, and butyric acid) and 4 non-volatile organic acid(i.e., fumaric acid, ${\alpha}-ketoglutaric$ acid, malic acid, and citric acid) were detected. The content of free amino acid was $596.3{\sim}1580.8$ mg%. Glutamic acid was most abundant component among the amino acids, 2nd abundant component was alanine, it's content was $79.9{\sim}165.3$ mg%, 3rd abundant component was leucine, it's count was $41.7{\sim}161.6$ mg%. Finally, essential amino acid content was revealed $33.2{\sim}40.38%$.

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