• Journal of Electrochemical Science and Technology
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• v.12 no.4
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• pp.458-464
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• 2021

Lithium ion batteries (LIBs) is a kind of rechargeable secondary battery, developed from lithium battery, lithium ions move between the positive and negative electrodes to realize the charging and discharging of external circuits. Zeolitic imidazolate frameworks (ZIFs) are porous crystalline materials in which organic imidazole esters are cross-linked to transition metals to form a framework structure. In this article, ZIF-67 is used as a sacrificial template to prepare nano porous carbon (NPC) coated cobalt nanoparticles. The final product Co/NPC composites with complete structure, regular morphology and uniform size were obtained by this method. The conductive network of cobalt and nitrogen doped carbon can shorten the lithium ion transport path and present high conductivity. In addition, amorphous carbon has more pores that can be fully in contact with the electrolyte during charging and discharging. At the same time, it also reduces the volume expansion during the cycle and slows down the rate of capacity attenuation caused by structure collapse. Co/NPC composites first discharge specific capacity up to 3115 mA h/g, under the current density of 200 mA/g, circular 200 reversible capacity as high as 751.1 mA h/g, and the excellent rate and resistance performance. The experimental results show that the Co/NPC composite material improves the electrical conductivity and electrochemical properties of the electrode. The cobalt based ZIF-67 as the precursor has opened the way for the design of highly performance electrodes for energy storage and electrochemical catalysis.

• Journal of Microbiology and Biotechnology
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• v.32 no.6
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• pp.740-748
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• 2022

As circ_UBE2D2 has been confirmed to have targeted binding sites with multiple miRNAs involved in septic acute kidney injury (SAKI), efforts in this study are directed to unveiling the specific role and relevant mechanism of circ_UBE2D2 in SAKI. HK-2 cells were treated with lipopolysaccharide (LPS) to construct SAKI model in vitro. After sh-circ_UBE2D2 was transfected into cells, the transfection efficiency was detected by qRT-PCR, cell viability and apoptosis were determined by MTT assay and flow cytometry, and expressions of Bcl-2, Bax and Cleaved-caspase 3 were quantified by western blot. Target genes associated with circ_UBE2D2 were predicted using bioinformatics analysis. After the establishment of SAKI rat model, HE staining and TUNEL staining were exploited to observe the effect of circ_UBE2D2 on tissue damage and cell apoptosis. The expression of circ_UBE2D2 was overtly elevated in LPS-induced HK-2 cells. Sh-circ_UBE2D2 can offset the inhibition of cell viability and the promotion of cell apoptosis induced by LPS. Circ_UBE2D2 and miR-370-3p as well as miR-370-3p and NR4A3 have targeted binding sites. MiR-370-3p inhibitor reversed the promoting effect of circ_UB2D2 silencing on viability of LPS-treated cells, but shNR4A3 neutralized the above inhibitory effect of miR-370-3p inhibitor. MiR-370-3p inhibitor weakened the down-regulation of NR4A3, Bax and Cleaved caspase-3 and the up-regulation of Bcl-2 induced by circ_UB2D2 silencing, but these trends were reversed by shNR4A3. In addition, sh-circ_UBE2D2 could alleviate the damage of rat kidney tissue. Circ_UBE2D2 mitigates the progression of SAKI in rats by targeting miR-370-3p/NR4A3 axis.

• The Journal of the Korea Contents Association
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• v.22 no.7
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• pp.89-101
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• 2022

COVID-19 is significantly changing consumers' demand and habits. In order to understand consumer characteristics and find effective advertising directions in the post-COVID-19 era, this study set young consumers who are more sensitive to market changes and technological transformation from a subjective perspective of advertising audiences. Through the Q methodology, the advertising development model in the post-COVID-19 era was derived exploratively by examining their cognitive status of advertisements in the post-COVID-19 era. The model consists of three types of advertisements: "demand mining online ads" that value consumer demand and adapt to online shopping paths, "added value creation experiential ads" that value derived value and consumer experiences, and "practical and sentimental value creative ads" based on pragmatism and emotional values. In addition, this study also suggested for the sustainable practice of advertising in the post-COVID-19 era in various aspects, such as "seeking multidimensional values," "expanding consumer experience," and "mining and leading demand.

• Advances in nano research
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• v.13 no.1
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• pp.87-96
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• 2022

Drug self-delivery systems can easily realize combination drug therapy and avoid carrier-induced toxicity and immunogenicity because they do not need non-therapeutic carrier materials. So, designing appropriate drug self-delivery systems for specific diseases can settle most of the problems existing in traditional drug delivery systems. Retinal pigment epithelium is very important for the homeostasis of retina. However, it is vulnerable to oxidative damage and difficult to repair. Worse still, the antioxidants can hardly reach the retina by non-invasive administration routes due to the ocular barriers. Herein, the targeted group (folic acid) and antioxidant (melatonin) have been grafted on the surface of ZnO quantum dots to fabricate a new kind of drug self-delivery systems as a protectant via eyedrops. In this study, the negative nanoparticles with size ranging in 4~6 nm were successfully synthesized. They could easily and precisely deliver drugs to retinal pigment epithelium via eyedrops. And they realized acid degradation to controlled release of melatonin and zinc in retinal pigment epithelium cells. Consequently, the structure of retinal pigment epithelium cells were stabilized according to the expression of ZO-1 and β-catenin. Moreover, the antioxidant capacity of retinal pigment epithelium were enhanced both in health mice and photic injury mice. Therefore, such new drug self-delivery systems have great potential both in prevention and treatment of oxidative damage induced retinal diseases.

• Structural Engineering and Mechanics
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• v.83 no.3
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• pp.341-351
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• 2022

During bolted flange assembly, the contact stiffness of some areas of the joint surface may be low due to the elastic interaction. In order to improve the contact stiffness at the lowest position of bolted flange, the correlation model between the initial bolt pre-tightening force and the contact stiffness of bolted flange is established in this paper. According to the stress distribution model of a single bolt, an assumption of uniform local contact stiffness of bolted flange is made. Moreover, the joint surface is divided into the compressive stress region and the elastic interaction region. Based on the fractal contact theory, the relationship model of contact stiffness and contact force of the joint surface is proposed. Considering the elastic interaction coefficient method, the correlation model of the initial bolt pre-tightening force and the contact stiffness of bolted flange is established. This model can be employed to reverse determine the tightening strategy of the bolt group according to working conditions. As a result, this provides a new idea for the digital design of tightening strategy of bolt group for contact stiffness of bolted flange. The tightening strategy of the bolted flange is optimized by using the correlation model of initial bolt pre-tightening force and the contact stiffness of bolted flange. After optimization, the average contact stiffness of the joint surface increased by 5%, and the minimum contact stiffness increased by 6%.

• Journal of Broadcast Engineering
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• v.26 no.6
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• pp.725-737
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• 2021

Although the performance of cameras is gradually improving now, there are noise in the acquired digital images from the camera, which acts as an obstacle to obtaining high-resolution images. Traditionally, a filtering method has been used for denoising, and a convolutional neural network (CNN), one of the deep learning techniques, has been showing better performance than traditional methods in the field of image denoising, but the details in images could be lost during the learning process. In this paper, we present a CNN for image denoising, which improves image details by learning the details of the image based on wavelet transform. The proposed network uses two subnetworks for detail enhancement and noise extraction. The experiment was conducted through Gaussian noise and real-world noise, we confirmed that our proposed method was able to solve the detail loss problem more effectively than conventional algorithms, and we verified that both objective quality evaluation and subjective quality comparison showed excellent results.

• Journal of the Korean Society of Industry Convergence
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• v.24 no.6_2
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• pp.849-860
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• 2021

Recently, it is easy to find cases of 3D printing product, equipment, and materials in the architecture field. However, there is a lack of distribution environment where 3D printing products can be traded on an online platform or to access on-demand services in the architecture field. Therefore, in this study, a distribution platform development plan was proposed in consideration of the maturity level of the 3D printing distribution market in the domestic architecture field. For this purpose, the research was carried out as follows. First, by analyzing the case of the 3D printing distribution platform, the development stage of the distribution platform was set as three stages from the perspective of market maturity, platform development level, and sales/purchase experience level of suppliers and consumers. Second, the market maturity of the current domestic architecture field was evaluated as the first stage, and a distribution platform that could be implemented in the first stage was presented as a pilot. Third, we presented the first stage pilot, collected practical opinions on future construction plans through in-depth interviews, and presented detailed implementation plans for each stage necessary to achieve the second and third stage market maturity goals. Based on the roadmap derived from this study, it is expected that the domestic distribution platform market will grow step by step in the future and be utilized for business model development.

• Journal of Microbiology and Biotechnology
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• v.32 no.9
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• pp.1178-1185
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• 2022

Steroids are a class of compounds with cyclopentane polyhydrophenanthrene as the parent nucleus, and they usually have unique biological and pharmacological activities. Most of the biosynthesis of steroids is completed by a series of enzymatic reactions starting from cholesterol. Synthetic biology can be used to synthesize cholesterol in engineered microorganisms, but the production of cholesterol is too low to further produce other high-value steroids from cholesterol as the raw material and precursor. In this work, combinational strategies were established to increase the production of cholesterol in engineered Saccharomyces cerevisiae RH6829. The basic medium for high cholesterol production was selected by screening 8 kinds of culture media. Single-factor optimization of the carbon and nitrogen sources of the culture medium, and the addition of calcium ions, zinc ions and citric acid, further increased the cholesterol production to 192.53 mg/l. In the 5-L bioreactor, through the establishment of strategies for glucose and citric acid feeding and dissolved oxygen regulation, the cholesterol production was further increased to 339.87 mg/l, which was 734% higher than that in the original medium. This is the highest titer of cholesterol produced by microorganisms currently reported. The fermentation program has also been conducted in a 50-L bioreactor to prove its stability and feasibility.

• Nuclear Engineering and Technology
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• v.53 no.5
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• pp.1556-1568
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• 2021

The plate-type fuel assembly adopted in nuclear research reactor suffers from complicated effect induced by non-uniform irradiation, which might affect stress conditions, mechanical behaviors and thermal-hydraulic performance of the fuel assembly. This paper is the Part II work of a two-part study devoted to analyzing the complex unique mechanical deformation and thermal-hydraulic characteristics for the typical plate-type fuel assembly under irradiation effect, which is on the basis of developed and verified numerical thermal-fluid-structure coupling methodology under irradiation in Part I of this work. The mechanical deformation, thermal-hydraulic performance and Mises stress have been analyzed for the typical plate-type fuel assembly consisting of support plates under non-uniform irradiation. It was interesting to observe that: the plate-type fuel assembly including the fuel plates and support plates tended to bend towards the location with maximum fission rate; the hot spots in the fuel foil appeared at the location with maximum thickness increment; the maximum Mises stress of fuel foil was located at the adjacent location with the maximum plate thickness increment et al.

• Wind and Structures
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• v.34 no.5
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• pp.437-450
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• 2022

Vortex-induced vibration (VIV) is a significant concern when designing slender structures with square cross sections. VIV strongly depends on structural dynamics and flow states, which depend on the conditions of the approaching flow and shape of a structure. Therefore, the effects of the angle of attack on the coupling effects of VIV for a square cylinder are expected to be significant in practice. In this study, the aerodynamic forces for a fixed and elastically mounted square cylinder were measured using wind pressure tests. Aerodynamic forces on the stationary cylinder are firstly discussed by comparisons of variation of statistical aerodynamic force and wind pressure coefficient with wind angle of attack. The coupling effect between the aerodynamic forces and the motion of the oscillating square cylinder by VIV is subsequently investigated in detail at typical wind angels of attack with occurrence of three typical flow regimes, i.e., leading-edge separation, separation bubble (reattachment), and attached flow. The coupling effect are illustrated by discussing the onset of VIV, characteristics of aerodynamic forces during VIV, and interaction between motion and aerodynamic forces. The results demonstrate that flow states can be classified based on final separation points or the occurrence of reattachment. These states significantly influence coupling effects of the oscillating cylinder. Vibration enhances vortex shedding, which creates strong fluctuations in aerodynamic forces. However, differences in the lock-in range, aerodynamic force, and interaction process for angles of attack smaller and larger than the critical angle of attack revealed noteworthy characteristics in the VIV of a square cylinder.