• 한국자원식물학회지
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• 제29권5호
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• pp.555-563
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• 2016

국내 농가에서 재배되고 있는 산초 및 초피유전자원의 형태학적 특성과 유전적 다양성을 분석하여 분자생물학적 분류와 유전자원평가에 활용하고자 하였다. 산초 수집계통간의 유전적 다형성은 증폭된 총 88개 밴드 중 85개의 다형성 밴드가 검출되어 96.5%였으며, 초피나무 수집계통은 총 58개 밴드 중 다형성 밴드는 35개로 60.3%의 다형성을 나타내었다. 산초나무 수집 계통의 UBC 861 프라이머 500 bp 영역과 UBC 862 프라이머 300 bp 영역에서 종 특이적인 밴드가 검출되었다. 신품종 육종의 기초자료로 활용하고자 유전적 유사도 지수를 산출한 결과, 총 32계통간의 유전적 유사도 지수는 최저 0.116에서 최고 0.816 사이로 산초나무의 종내 유전적 유사도 지수는 0.177∼0.780, 초피나무의 종내 유전적 유사도 지수는 0.250∼0.816 사이로 낮게 나타나 교배육종 소재로 활용이 기대된다. 군집분석 결과, 유전적 유사도 지수 0.302에서 산초나무와 초피나무 수집 계통이 분리되어 산초나무 2개 그룹과 초피나무 1개 그룹으로 유집되었다. 반면 유집된 그룹과 형태학적 특성과 연관성은 없었다. 본 연구 결과를 바탕으로 ISSR 마커로 산초와 초피의 종간 구분 마커 개발이 기대되며, 유전적 유사도를 바탕으로 교배육종 소재 선발의 가능성이 평가되었다.

• 한국약용작물학회지
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• 제16권4호
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• pp.273-278
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• 2008

We aimed to investigate the antiviral activity of Zanthoxylum species against influenza virus A/WS/33, A/PR/8 and B/Lee/40 used by sulforhodamine B (SRB) assay and the action of leaves extracts of Zanthoxylum piperitum on life cycle of influenza virus A/WS/33. Among the twelve extracts, only the leaf extract of Z. piperitum exhibited strong antiviral activity at low concentration of less than 10${\mu}g/m{\ell}$ with no citotoxicity (50${\mu}g/m{\ell}$) against all of three viruses. In addition, only oseltamivir showed antiviral activity with $IC_{50}$ of 65.3${\mu}g/m{\ell}$ against influenza A/WS/33 among the viruses. Furthermore, the leaf extract of Z. piperitum suppressed infection of influenza virus A/WS/33, when added just prior (-1 hr) or after virus inoculation (0 hr). Leaf extract of Z. piperitum directly affect the infectivity of influenza virus A/WS/33 particles. Therefore, Leaf extract of Z. piperitum exhibited higher antiviral activity against three influenza viruses than that of the oseltamivir, which directly interacts with influenza A/WS/33 particles, affecting the initial stages of infection such as receptor binding and virus entry.

• 생약학회지
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• 제33권2호통권129호
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• pp.75-80
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• 2002

The Korean folk medicine 'Cho Pi Na Mu' has been used orally to tuberculosis, neuralgia, acute and chronic gastritis and arthritis. In order to clarify the botanical origin this crude drug, comparative anatomical studies were made on the branches of Zanthoxylum species growing wild in Korea, i.e. Z. coreanum, Z. piperitum, Z. piperitum var. pubescens, Z. planispinum, Z. schinifolium, Z. schinifolium var. inermis, Z. schinifolium var. microphyllum. As a result, it was clarified that 'Cho Pi Na Mu' was the branch of Z. piperitum.

• 한국미생물·생명공학회지
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• 제27권3호
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• pp.179-183
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• 1999

The volatile components (essential oil) showing antimicrobial activity were extracted from the fruit peel of Zanthoxylum piperitum DC by distillation and separated by thin layer chromatography (TLC). The crude volatile components exhibited antimicrobial activity only at very high concentration. The active fraction obtained by TLC inhibited noticeably the growth of bacteria. The minimum inhibitory concentration (MIC) of the fraction were 150ppm, 300ppm, and 300ppm against Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis, respectively. The components in the active fraction were identified by gas chromatography/mass spectrometry to be geranlyl acetate (60.23%), citronellal(36.01%), citronellol(3.77%), geraniol(0.46%), and cumin ldehyde(0.43%).

• 한국식품저장유통학회지
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• 제7권2호
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• pp.195-200
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• 2000

Yields of various solvent extracts for Zanthoxylum schinifolium and Z. piperitum leaves were higher in ethanol extract layer. Ethanol Extract in sancho and chopi leaves was fractionated with different solvents, such as hexane, chloroform, ethyl acetate, butanol and water, yields of hexane fraction was higher. In the solvent extracts using methanol, ethanol, hexane, chloroform, and water, ethanol extract showed the most effective antimicrobial activities. Ethanol extract in sancho and chopi leaves was fractionated, the most antimicrobial activities of sancho leaf were ethyl acetate fraction, and chopi leaf were chloroform fraction.

• 한국식품과학회지
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• 제21권4호
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• pp.562-568
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• 1989

9월 중에 채취한 성숙한 초피 (Zanthoxylum piperitum DC)의 과피와 잎성분의 변성을 고려한 방법인 gas co-distillation으로 추출하고, GC/MS와 함께 workstation BASIC program들을 사용하여 극성이 다른 두 column(FFAP, HP-1)에 대한 authentic standard들의 retention index(RI) 값들을 구하여 HP-1 및 FFAP, RI reference library를 만든 후 초피의 정유성분들의 두 column library 탐색을 병행하여 분석함으로써, 과피에서 myrcene외 14 hydrocarbon들, linalool외 8 alcohol들, citronellal외 3 aldethyde들, carvone외 2 ketone들, methyl salicylate외 7 ester들 그리고 1,8-cineol 외 3 oxide를 확인하였고, 잎에서 limonene외 7 hydrocarbon들, citronellol외 7 alcohol들, cumin aldehyde외 1 aldehyde, carvone, 그리고 estragole외 1 oxide를 확인하였다. 과피에서는 1,8-cineol(25.47%), limonene(11.9%), geranylacetate(9.01%), myrcene(6.15%), geraniol(2.88%), citronellal(2.25%)등이 주성분이었고, 잎에서는 citronellal(23.11%), 1,8-cineol(18.38%), citronellol(6.04%), methyl cinnamate(4.08%)등이 주성분이었다.

• Food Science and Biotechnology
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• 제14권4호
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• pp.529-532
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• 2005

Volatile compounds, isolated from Chopi (Zanthoxylum piperitum A.P. DC.) using steam distillation, were analyzed by gas chromatography/mass spectrometry-olfactometry (GC-MS-O). Forty-six volatile compounds, consisting of 12 hydrocarbons, 8 aldehydes, 5 esters, 12 alcohols, 4 ketones, 4 oxides and 1 acid, were tentatively identified from the essential oil of Chopi. Unidentified compounds constituted 7.2% of the total peak area. Limonene was the most abundant compound, followed by geranyl acetate, citronellal, cryptone and ${\beta}$-myrcene. In addition, aroma-active compounds, in particular citronellal and limonene, which are related to the citrus and Chopi flavors of Chopi essential oil, were detected. The aroma of Chopi essential oil had a score of 4.8 on the preference test (neither like nor dislike) and a score of 5.97 on the intensity test (slightly strong) using the 9-point hedonic scale.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1371-1378
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• 2009

The aim of this study is to elucidate the anti-inflammatory activities of chopi (Zanthoxylum piperitum A.P. DC.) essential oil. Essential oil (EO) of chopi was extracted by steam distillation method, and its major constituents were limonene and geranyl acetate. Chopi-EO decreased approximately 38% of nitrite production, as compared to the lipopolysaccharde (LPS)-induced nitrite production. However, chopi-EO and its components did not quench nitric oxide (NO) chemically in cellfree system, and markedly inhibited approximately 40.4% of inducible nitric oxide synthase (iNOS) mRNA transcription. In addition, the inhibition of E-selectin gene transcription by chopi-EO caused the suppression of cellular adhesion. These results suggest that chopi-EO may exert potential anti-immunological inflammatory activity.

• 대한화학회지
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• 제53권5호
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• pp.613-619
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• 2009

• 한국식품영양과학회지
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• 제22권5호
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• pp.617-620
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• 1993

한국산 초피나무의 물고기에 대한 독성력을 확인하기 위하여 물질을 용매추출, silica gel column chromatography와 재결정을 통하여 분리 정제하였다. 추출물의 물고기에 대한 독성실험 결과, methanol 추출물에서 가장 강한 독성이 나타났으며, 그 다음으로는 물추출물, 초피의 건조분말 그리고 chloroform추출물 순서로 독성을 보였다. 이 추출물의 구조를 기기분석을 통해 해석한 결과 L-asarinin 임을 확인하였다.