• 한국식품과학회지
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• 제48권4호
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• pp.392-397
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• 2016

천연물 유래 단일 성분의 항염증 효과에 대한 잠재성을 평가하는 스크리닝의 일환으로 11종의 단일 물질을 대상으로 항염증 효과를 탐색한 결과, 단풍취에서 분리정제한 zaluzanin C의 산화질소(II) 생성 억제능이 뛰어난 것을 확인하였다. 따라서 본 연구에서는 단풍취에서 분리정제한 zaluzanin C가 지질다당류로 자극한 마우스 대식세포인 RAW264.7 세포에서 염증반응에 미치는 영향에 대해 평가하고, 관련 메커니즘에 대해 검토하였다. Zaluzanin C는 LPS 자극에 의해 유도된 iNOS 단백질 발현양을 감소시킴으로써 산화질소(II) 생성을 억제할 뿐만 아니라 IL-6와 같은 염증 유발 사이토카인의 분비를 억제하였다. 이러한 효과는 전사인자인 NF-kB의 세포질에서 핵으로의 이동을 억제함으로써 나타나는 것으로 판단된다. 이러한 결과로부터, zaluzanin C가 염증 반응을 저해하는 효과가 있는 것으로 나타나, 향후 염증성 질환을 예방, 개선 및 치료하는데 유용한 물질로 사용될 가능성이 있을 것으로 생각된다. 하지만 이를 위해서는 zaluzanin C의 생체 내 이용률 및 생리적 활성 농도 등에 대한 추가 연구가 필요한 것으로 생각된다.

• 한국어병학회지
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• 제37권1호
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• pp.133-138
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• 2024

This study is a report on combinational effects between four sesquiterpene lactones (SLs) from bay laurel (Laurus nobilis) leaves, and oxytetracycline (OTC) or amoxicillin (AMX) against four fish pathogenic bacteria such as Vibrio anguillarum, V. harveyi, Edwardsiella tarda, and Streptococcus iniae. Individually, four SLs exerted little antibacterial activity against fish pathogenic bacteria. However, when combined with OTC or AMX, they showed synergistic interaction against pathogenic bacteria. Especailly, zaluzanin C (1) reduced the MIC of OTC (or AMX) eight-fold. Our results showed that combinations of SLs with antibiotics (ABTs) are more effective than ABTs alone to control pathogenic bacteria. The highest synergistic effect was observed when zaluzanin C (1) was combined with OTC or AMX against V. harvey or S. iniae, displaying significant reductions of MICs up to 8-fold (0.125 to 0.015 ㎍/mL and 0.0078 to 0.0009 ㎍/mL). In addition, zaluzanin C (1) improved the antibiotic potency of OTC against OTC resistant V. harveyi (250 ㎍/mL to 62.5 ㎍/mL). Synergism between ABTs and phytochemical such as SLs could be a therapeutically helpful concept to improve the efficacy of ABTs and prevent antibiotic resistance. These results suggest that SLs can be used as an alternative to reduce antibiotic resistance in aquaculture.

• 생약학회지
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• 제31권2호
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• pp.125-129
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• 2000

Three sesquiterpene lactones (1, 2 and 5) and two lipid glycerols (3 and 4) were isolated from MeOH extract of Ainsliaea acerifolia (Compositae). Based on spectral data, their chemical structures were determined as estafiatone (1), zaluzanin C (2), 3-O-(9Z, 12Z, 15Z-octadecatrienoyl) glycerol (3), 3-O-(9Z, 12Z-octadecadienoyl) glycerol (4) and glucozaluzanin C (5). Compounds 1, 3 and 4 were previously not reported from Ainsliaea species.

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 춘계총회 및 학술대회
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• pp.150.2-151
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• 2000

• Archives of Pharmacal Research
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• 제29권3호
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• pp.203-208
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• 2006

The chromatographic separation of the MeOH extract of the roots of Ainsliaea acerifolia (Compositae) led to the isolation of six known terpenes and two known lignans. Their structures were identified by spectroscopic methods as mokko lactone (1), betulonic acid (2), betulinic acid (3), zaluzanin C (4), $1{\beta}-hydroperoxygermacra-4(15)$, 5, 10(14)-triene (5), pluviatilol (6), (+)-syringaresinol (7), and glucozaluzanin C (8). Compounds $1{\sim}4$ and 8 showed non-specific significant cytotoxicity against five human tumor cell lines with $ED_{50}$ values ranging from $0.36{\sim}5.54{\mu}g/mL$.

• Archives of Pharmacal Research
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• 제29권11호
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• pp.937-941
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• 2006

Ixeris dentata forma albiflora was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_{2}O$. Eight sesquiterpenes were isolated through repeated silica gel and octadecyl silica gel ($C_{18},\;ODS$) column chromatography of the EtOAc and n-BuOH fractions. Physicochemical analysis using NMR, MS and IR revealed the chemical structures of the sesquiterpenes, which were zaluzanin (1), 9a-hydroxyguaian-4(15), 10(14), 11 (13)-triene-6, 12-olide(2), $3{\beta}-O-{\beta}-D-glucopyranosyl-8{\beta}-hydroxyguaian$-4(15), 10(14)-diene-6, 12-olide (3), $3-O-{\beta}-D-glucopyranosyl-8{\beta}-hydroxyguauan$-10(14)-ene-6, 12-olide (4), ixerin M (5), glucozaluzanin C (6), crepiside I (7), and ixerin D (8). This is the first time that these sesquiterpene lactones have been isolated from this plant. Compounds 1, 2 and 7 revealed relatively high cytotoxicities on human colon carcinoma cell and lung adeno-carcinoma cell, while compounds 5 and 7 showed acyl-CoA: cholesterol acyltransferase (ACAT) inhibitory activity.

• Applied Biological Chemistry
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• 제47권2호
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• pp.251-257
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• 2004

식용식물자원으로부터 활성소재를 찾기 위하여 흰씀바귀 뿌리를 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH및 $H_2O$로 용매 분획하였다. EtOAc와 n-BuOH 분획에 대하여 column chromatography를 반복하여 4종의 화합물을 분리하였다. 각각에 대하여 2D-NMR을 포함한 스펙트럼 데이터의 해석과 문헌 자료를 조사하여 zaluzanin C (1), $9{\alpha}-hydroxyguaian-4(l5),10(14),11(13)-triene-6,12-olide$ (2), $3{\beta}-O-{\beta}-D-glucopyranosyl-8{\beta}-hydroxyguaian-4(15),\;10(14 )-diene-6,12-olide$ (3), $3-O-{\beta}- D-glucopyranosyl-8{\beta}hydroxyguaian-10(14)-ene-6,12-olide$ (4)로 구조를 결정하였다. 이들 화합물에 대하여 ACAT(Acyl-CoA: cholesterol acyltransferase), DGAT (diacylglycerol acyltransferase) 및 FPTase(farnesyl-protein transferase)의 활성에 미치는 억제효과를 측정하였다. Compound 1과 Compound 2는 DGAT에 대한 활성억제효과에 있어서 $IC_{50}$ 값이 각각 0.13 mM, 0.10 mM로 나타났고, FPTase에 대하여는 각각 0.15 mM, 0.18 mM로 나타났으며, ACAT에 대하여는 약한 억제 활성을 나타냈다. 따라서 흰씀바귀는 항암 및 항고혈압의 소재 개발에 있어서 유용한 자원으로 활용될 수 있을 것이다.