• 한국환경보건학회지
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• 제40권1호
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• pp.55-62
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• 2014

Objectives: $^{32}P$-postlabeling assay is the most sensitive method of detecting DNA adduct formation. However, it is limited by a low sample throughput and use of radioisotopes (RI). In this study, we modified it to minimize these limitations and applied it to Z. platypus exposed to Benzo(a)pyrene (BaP) in order to investigate DNA adduct formation (effect biomarker for pollutants) in Z. platypus for assessing risk of waterborne BaP exposure. Methods: DNA hydrolysis was performed only with Micrococcal nuclease (MNase), RI reduction test was performed and the overlapping steps between thin layer chromatography (TLC) and radioisotope high-performance liquid chromatography (RI-HPLC) were omitted. The application of a modified method to Z. platypus exposed to BaP was performed. Results: The results revealed that the amount of RIs used can be reduced roughly 10-fold. Because the analysis time was shortened by 8.5 hours, the sample throughput per hour was increased compared with the previous method. The results of applying modified $^{32}P$-postlabeling assay to Z. platypus, DNA adduct formation in Z. platypus showed dose-dependency with the BaP concentration. Only BPDE-dGMP was detected as a DNA adduct. Conclusion: These results demonstrate that the modified $^{32}P$-postlabeling assay is a suitable method for detecting DNA adduct formation in Z. platypus exposed to waterborne BaP and will be useful in risk assessment of carcinogenic effect in aquatic environment due to BaP.

• 대한전자공학회논문지
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• 제22권5호
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• pp.69-74
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• 1985

본 논문에서는 먼저 Sensitivity가 가장 낮은 LC Ladder 필터로 부터 FDNR frequency dependent negative resists.) 방식을 이용하여 5차 Butterworth 저역 통과 능동 RC필터를 설계하였으며 능동 RC 필터를 SCF(switched capacito, filter)로 변조하는 과정에서 Bilinear Z변환을 이용하였다. Bilinear Z 변환으로 설계된 SCF에서 aliasing 현상은 피할수 있으나 주파수 대곡이 발생한다. 아나로그 필터를 디지틀 릴터로 변환하면 이러한 영향으로 속단 주파수 영역이 일치하지 않으므로 이것을 적게 하기 위하여 Prewarping을 해준다. 그 결과 Prewarping된 SCF는 Bilinear Z 변환된 SCF보다 각파수 이유이 현저하게 개선됨을 확인하였다.

• Korean Journal of Mathematics
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• 제23권2호
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• pp.231-248
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• 2015

In this paper, we solve the following quadratic $\rho$-functional inequalities ${\parallel}f(\frac{x+y+z}{2})+f(\frac{x-y-z}{2})+f(\frac{y-x-z}{2})+f(\frac{z-x-y}{2})-f(x)-x(y)-f(z){\parallel}\;(0.1)\\{\leq}{\parallel}{\rho}(f(x+y+z+)+f(x-y-z)+f(y-x-z)+f(z-x-y)-4f(x)-4f(y)-f(z)){\parallel}$ where $\rho$ is a fixed complex number with ${\left|\rho\right|}<\frac{1}{8}$, and ${\parallel}f(x+y+z)+f(x-y-z)+f(y-x-z)+f(z-x-y)-4f(x)-4f(y)-4f(z){\parallel}\;(0.2)\\{\leq}{\parallel}{\rho}(f(\frac{x+y+z}{2})+f(\frac{x-y-z}{2})+f(\frac{y-x-z}{2})+f(\frac{z-x-y}{2})-f(x)-f(y)-f(z)){\parallel}$ where $\rho$ is a fixed complex number with ${\left|\rho\right|}$ < 4. Using the fixed point method, we prove the Hyers-Ulam stability of the quadratic $\rho$-functional inequalities (0.1) and (0.2) in complex Banach spaces.

• ALGAE
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• 제20권4호
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• pp.379-388
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• 2005

Seagrass bed is an important component in coastal and estuarine ecosystems, providing food and shelter to a wide variety of fauna. Recently, seagrass coverage has declined significantly due to anthropogenic influences such as reclamation, dredging, and eutrophication and consequently, necessity of seagrass habitat restoration is rising. Transplantation experiments with Zostera marina using TERFS, staple method, and shell method have been conducted at Dadae Bay, Kosung Bay and Jindong Bay on the south coast of Korea to select an optimal transplanting method for restoration of Z. marina habitat. Three experimental sites located at the vicinity of natural Z. marina beds with an average water depth of about 4m. Z. marina plants, which were collected from donor bed in Koje Bay were also transplanted at 7 different time from October 2003 to July 2004 to find appropriate transplanting time. Density of Z. marina was monitored monthly at both transplanted areas and natural beds. Transplantation using the staple method showed the highest survival rate of transplant. Shell method was also an effective transplanting method at muddy areas in Kosung Bay and Jindong Bay, but not suitable at sandy areas in Dadae Bay. These results suggest that sediment composition of transplanting areas should be considered for the selection of the optimal transplanting method. Z. marina transplanted during fall usually showed the highest survival rate, while most Z. marina plants transplanted in summer died due to high lethal temperature during this period.

• Journal of Advanced Marine Engineering and Technology
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• 제26권2호
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• pp.219-225
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• 2002

Parameter tuning methods by Ziegler-Nichols for PID controllers are generally classified into Z-N(1) and Z-N(2). The purpose of this paper is to describe what relations exist between the methods of Z-N(1) and Z-N(2), or how Z-N(1) can be originated from Z-N(2) by analyzing one loop control system composing of P or PI controller and time delay process. In this paper, for the first step to seek mutual relations, the simple formulas of Z-N(2) are transformed into those composing of the same parameters as Z-N(1) which is derived from the analysis of frequency characteristics. Then, the approximation of the actual ultimate frequency is proposed as important premise in the translation between Z-N(1) and (2). Such equalization and approximation brings a simple approximated formula which can explain how Z-N(1) is originated from the Z-N(2) in the form of formula.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 춘계학술대회 논문집 전기기기 및 에너지변환시스템부문
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• pp.449-451
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• 2001

This study presents a study on modified algorithm to operating train simulation of AT feeding systems. In this study equivalent impedance is constructed by $Z_c,\;Z_r,\;Z_f,\;Z_{cr},\;Z_{rf},\;Z_{fc}$. The train current flows through the all auto-transformer corresponding to track impedance. To calculate train voltage from analyzing the railway systems, the algorithm is based on the K. C. L, K. V. L, superposition and circuit separation method. Multi-train's voltages are determined by calculating the catenary voltage at each train's position and adding up these train's voltage drop. Case studies use a field operational data, show that the proposed method is easily applied.

• 충청수학회지
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• 제31권1호
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• pp.29-42
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• 2018

In this paper, we investigate the stability of two functional equations f(ax+by + cz) - abf(x + y) - bcf(y + z) - acf(x + z) + bcf(y) - a(a - b - c)f(x) - b(b - a)f(-y) - c(c - a - b)f(z) = 0, f(ax+by + cz) + abf(x - y) + bcf(y - z) + acf(x - z) - a(a + b + c)f(x) - b(a + b + c)f(y) - c(a + b + c)f(z) = 0 by applying the direct method in the sense of Hyers and Ulam.

• 전자공학회논문지A
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• 제31A권6호
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• pp.26-33
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• 1994

Much time and labor has been conventionally paid for fine adjustment of circuit elements by experience to improve the characteristics of Tap-off for CATV systems. To solve the problem, in this paper, the relations between the even- and odd-mode impedance. Z$_{oe}$ and Z$_{oo}$ of symmetrical 4-port coupled-line directional coupler and power dividing characteristics of Tap-off for CATV system have been derived. After the coupling, the reflectivity and the isolation are represented by Z$_{oe}$ and Z$_{oo}$ only, the fine adjustment of all S-parameters can be performed by controling the elements of Z$_{oe}$ and Z$_{oo}$ only. Furthermore, the validity of the new fine adjustment method proposed here has been confirmed by experiments.

• Steel and Composite Structures
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• 제51권3호
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• pp.225-241
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• 2024

For several reasons, cold-formed steel (CFS) beams are often manufactured with holes. Nevertheless, because of holes, the reduction in the web area causes a decrease in the bending strength. Edge stiffeners are presently added around the holes to improve the bending strength of flexural members. Therefore, this research studies CFSZ-beams with stiffened holes and investigates how edge stiffener affects bending strength and failure modes. Nonlinear analysis was carried out using ABAQUS software and the developed finite element (FE) model was verified against tests from previous studies. Using the verified FE model, a parametric study of 104 FE models was conducted to investigate the influence of key parameters on bending strength of Z- sections. The results indicated that the effect of holes is less noticeable in very thin Z-sections. Moreover, adding edge stiffeners around the holes improves the flexural capacity of Z-beams and sometimes restores the original bending capacity. Because the computational techniques used to solve the CFS buckling mode with stiffened holes are still unclear, a numerical method using constrained and unconstrained finite strip method (CUFSM) software was proposed to predict the elastic distortional buckling moment for a wide variety of CFSZ-sections with stiffened holes. A numerical method with two procedures was applied and validated. Upon comparison, the numerical method accurately predicted the distortional buckling moment of CFS Z-sections with stiffened holes.

• 소음진동
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• 제7권1호
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• pp.135-142
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• 1997

This paper presents a digital modeling technique of the distributed system. The basic idea of the proposed technique is to discretize a continuous system with respect to the spatial coordinates using bilinear method. The response of the discretized system is analyzed by Laplace transform and z-transform. The computational results in torsional shaft and Timoshenko beam using the proposed technique are compared with the exact solutions and the results of finite element method.