• Ecology and Resilient Infrastructure
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• v.1 no.2
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• pp.49-60
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• 2014

Fish assemblages play an integral role in stream ecosystem and are influenced by stream environmental conditions and habitat disturbances. Fish community structures and habitat parameters of U.S. EPA rapid bio-assessment protocol were surveyed to investigate the effect of stream environment and habitat disturbance on fish communities at 13 study sites in the Gapyeong Stream, a typical gravel-bed stream. Principal component analysis (PCA) based on data from habitat assessment at each study site indicated that the study sites were differentiated by habitat parameters such as embeddedness, velocity/depth regime and sediment deposition, which were related with bed slope. A total of 46 species belonging to 12 families were collected in the Gapyeong Stream. A dominant species was Zacco koreanus, subdominant species was Z. platypus. Hierarchical cluster analysis based on species abundance classified fish communities into the three main groups along the stream longitudinal change. Non-metric multidimensional scaling (NMDS) portrayed that fish community structures were related to major habitat parameters, i.e., epifaunal substrate/available cover, embeddedness, velocity/depth regime, sediment deposition, channel alternation and frequency of riffles. These results suggested that fish community structures were primary affected by the longitudinal environmental changes, and those were modified by habitat disturbance in the Gapyeong Stream, a gravel-bed stream.

• Journal of Environmental Impact Assessment
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• v.20 no.2
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• pp.207-215
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• 2011

In order to establish fundamental data for stream restoration and environmental flow, we investigated physical habitat conditions of Korean chub (Zacco koreanus) in the typical streams of Han River. Field monitoring including fish sampling was conducted from September 2008 to April 2010. A total number of fish caught in the 8 sites was 3,421 representing 8 families 31 species, and 17 species (54.8%) including Korean shinner (Coreoleuciscus splendidus) and Z. koreanus were Korean endemic species during the study period. The most frequently found species in number was pale chub (Z. platypus, 25.7%) followed by Z. koreanus (22.8%) and Microphysogobio longidorsalis (16.8%). Numbers of Z. Koreanus ranged from 8 to 10 cm of total length were the highest in size distribution of their population in all sites. They were widely found in ranges of flow velocity (0.2~0.9 m/sec, 89.6%), water depth (0.3~0.9 m, 91.6%), and different types of substrates except for silt, and they tended to prefer run (58.1%) and riffle (33.7%) with cobble bed (47.0%) microhabitat. Most of upper streams in Korea consist of riffles and runs that are repeatedly followed by another one. However, stream channelization and leveling have caused reduction of habitat diversity. Therefore, it is necessary to make an effort on stream rehabilitation with evaluation of physical habitat condition by indicator species in order to maintain biodiversity and perform ecological restoration.

• Molecules and Cells
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• v.27 no.2
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• pp.205-209
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• 2009

The loci of the 5S and 45S rRNA genes were localized on chromosomes in five species of Capsicum, namely, annuum, chacoense, frutescens, baccatum, and chinense by FISH. The 5S rDNA was localized to the distal region of one chromosome in all species observed. The number of 45S rDNA loci varied among species; one in annuum, two in chacoense and frutescens, and chinense, and four in baccatum, with the exceptions that 'CM334' of annuum had three loci and 'tabasco' of frutescens gad one locus. 'CM334'-derived BAC clones, 384B09 and 365P05, were screened with 5S rDNA as a probe, and BACs 278M03 and 262A23 were screened with 25S rDNA as a probe. Both ends of these BAC clones were sequenced. FISH with these BAC probes on pachytenes from 'CM334' plant showed one 5S rDNA locus and three 45S rDNA loci, consistent with the patterns on the somatic chromosomes. The 5S rDNA probe was also applied on extended DNA fibers to reveal that its coverage measured as long as 0.439 Mb in the pepper genome. FISH techniques applied on somatic and meiotic chromosomes and fibers have been established for chili to provide valuable information about the copy number variation of 45S rDNA and the actual physical size of the 5S rDNA in chili.

• Parasites, Hosts and Diseases
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• v.56 no.5
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• pp.509-513
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• 2018

Present study was performed to survey infection status of digenetic trematode metacercariae in 2 alien fish species, Micropterus salmoides (largemouth bass) and Lepomis macrochirus (bluegill), in 2 rivers draining Chungcheongbuk-do, Republic of Korea. A total of 107 largemouth bass and 244 bluegills were caught in Daecheong-ho (ho=lake) and Musim-cheon (a branch of Geum-gang), in Chungcheongbuk-do April-July 2015. Additionally, 68 native fish of 5 species, i.e., Zacco platypus, Hemibarbus longirostris, Carassius auratus, Pseudogobio esocinus and Puntungia herzi, were caught from the same water bodies. All of the fish collected were examined by artificial digestion method. The metacercariae of Centrocestus armatus, Clinostomum complanatum, Metagonimus sp. and Diplostomum spp. were detected from 4 out of 5 native fish species in Daecheong-ho. However, any metacercariae were not found from 87 M. salmoides and 177 L. macrochirus in Daecheong-ho. In Musim-cheon, metacercariae of Exorchis oviformis and Metacercaria hasegawai were detected from 78% Z. platypus and 34% L. macrochirus, but any metacercariae not found in M. salmoides. We report here that the 2 alien fish species were less infected with the metacercariae than the native ones. Surveys on the metacercariae in the alien fish species in geographically various rivers should be undertaken for better understanding on the role of alien fish species in the trematode infections in Republic of Korea.

• Korean Journal of Head & Neck Oncology
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• v.26 no.1
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• pp.27-29
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• 2010

Tracheostomal stenosis after total laryngectomy is brothersome problem to surgeon and patient. To prevent tracheostomal stenosis, tension around the tracheostoma must be released. Advancement flap, V-Y inset and Z-plasty were suggested as solution to tracheostomal stenosis. But such methods need elevation of superior flap and it is very difficult when the patients received radiotherapy and has high change of developing pharyngocutaneous fistula. We suggested new stomaplasty technique which named "Modified Fish-Mouth Technique" that does not need to elevate superior skin flap.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2009.06a
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• pp.299-300
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• 2009

• Microbiology and Biotechnology Letters
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• v.13 no.4
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• pp.409-415
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• 1985

This study has been carried out in order to investigate the bacterial quality of fish meat paste products and the characteristics of isolated thermodurics from the products. Twenty samples of crab-flavored fish stick (Kematsal), 23 samples of plate fish meat paste (Panomuk, Kamaboko), 5 samples of fried fish meat paste (Tigimomuk), 2 samples of roasted fish meat paste (Puduromuk, Chikuwa), 20 samples of fish sausage were collected from processing plants and supermarkets in Pusan, Korea during the period from May to October in 1984. The results obtained are as follows. Amont the samples collected from supermarkets, roasted fish meat paste and fried fish meat paste marked hish counts in coliforms and fungi while very low in the samples of crab-flavored fish stick and plate fish meat paste. Salmonella was not detected in all the samples examined and Staphylococcus aureus was detected only in fried fish meat paste, Thermoduric bacteria were detected less than 10$^2$/g in the samples of crab-flavored fish stick and plate fish meat paste, which might come from subsidiary materials such as starch and seasonings. Among the isolated bacteria, distribution of the proteolytics were more than 87％ and the lipolytics were less than 20％. Gram positive bacteria was more than 70％ in crab-flavored fish stick and plate fish meat paste, 47.3％ in fried fish meat paste. And rod in shape was almost more than 90％ in all the samples. The most heat resistant bacterium isolated from the samples was identified as a Bacillus licheniformis(named B. licheniformis CR-11). The strain showed strong proteolytic activity and also grew well at above 2$0^{\circ}C$. The growth rate and generation time of CR-11 strain were 0.31 hr$^{-1}$ , 2.24 hr at 2$0^{\circ}C$, 0.64 hr$^{-1}$ , 1.09 hr at 3$0^{\circ}C$ and 0.78 hr$^{-1}$ , 0.89 hr at 35$^{\circ}C$. Heat resistance value of the spores of CR-11 strain suspended in phosphate buffer solution was D$_{85}$ $^{\circ}C$=41.9 min, D$_{90}$ $^{\circ}C$=27.9 min, D$_{95}$ $^{\circ}C$=10.2 min, D$_{100}$ $^{\circ}C$=4.3 min (Z=13.8$^{\circ}C$)

• Journal of Korean Society on Water Environment
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• v.28 no.4
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• pp.512-522
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• 2012

This study is focused on fish fauna and distributional features along with physical gradients and stream orders in the Tamjin River basin, Korea. Fish sampling and investigation were conducted for 2 years from 2009 to 2010. Total number of fish species collected were 47 species of 16 family. The group of cyprinidae was mostly abundant. Both fish abundance and biotic index were declined with increasing altitude and longitudinal distance from the river mouth. Two species of Zacco temminckii and Pungtungia herzi were prevalent in most of sampling sites and showed no altitudinal and longitudinal differences. Most other species, however, were spatially restricted to specific regional altitudes. Several species including Zacco platypus, Squalidus gracilis majimae and P. herzi were broadly distributed from river mouth to 50 km. Eight species including Z. temminckii and Rhynchocypris oxycephalus only appeared in over 10 km from the river mouth. Both Odontobutis platycephala and Pseudobagrus koreanus were classified as "upstream dewelling species" observed only in over 30 km from the river mouth. High proportion of tolerant and omnivorous species were more dominated in the downstream zone of the dam, whereas intolerant and insectivorous species were abundant in the upstream zone of the dam. The variable of stream order showed positive relationship between number of fish species and total individuals collected. Sensitive species including insectivore had a declining tendency with increasing stream order, while tolerant specie including omnivore species had relatively increased with stream order. Overall, our study suggested that fish distribution considerably depends on altitudinal gradient and longitudinal distance from the river mouth as well as physical habitat.

• Korean Journal of Environment and Ecology
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• v.27 no.6
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• pp.695-703
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• 2013

The fish fauna and population Zacco koreanus in the Cheonggye stream after the rehabilitation was investigation from April 2011 to May 2012. The collected species during the survey period were 24 species belonging to three families. Among them, Korean endemic species such as Rhodeus uyekii, Acheilognathus yamatsutae, Acheilognathus gracilis, Squalidus japonicus coreanus and Zacco koreanus were observed in the stations. Dominant species was Zacco platypus (St. 1, 3, 4) and Z. koreanus (St. 2). A total of species of A. gracilis, Hemibarbus longirostris, Gnathopogon strigatus, S. japonicus coreanus, Pseudogobio esocinus, Erythroculter erythropterus, and Tridentiger brevispinis were first observed this study with compared the previous data (2005, 2008). It seems that the introduction species of artificially to the Cheonggye stream were Cyprinus carpio (fancy carp), Carassius auratus (gold fish), Carassius auratus (chinese carp), Zacco temmincki, Z. koreanus and Misgurnus mizolepis. Calculation of the length-weight relationship of Z. koreanus showed the value of constant a as 0.000007 and b as 3.58, and condition factor (K) was 0.82 on average. The frequency analysis of total length indicated that the group 20~80mm in total length is represented by one year-old individuals, the group 90~120mm by two year-old individuals, and the group over 130mm by three year-old individuals. It is assessed that stable spawning occurred following the introduction to the Cheonggye stream.

• Ecology and Resilient Infrastructure
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• v.1 no.1
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• pp.39-48
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• 2014

In order to establish fundamental data for stream restoration and environmental flow, we investigated optimal ecological flowrate (OEF) and riverine health condition in the Chogang Stream, a tributary to Geum River, Korea. The number of fish individuals sampled in this period were 4,669 in 36 species of 9 families. The most abundant species was Korean chub (Zacco koreanus, 34.0%) followed by pale chub (Z. platypus, 22.6%) and Korean shinner (Coreoleuciscus splendidus, 13.3%). Index of biological integrity (IBI) and qualitative habitat evaluation index (QHEI) values decreased from upstream to downstream along the stream. The estimated IBI value ranged from 27.9 to 38.6 with average 32.2 out of 50, rendering the site ecologically fair to good health conditions. OEF was estimated by the physical habitat simulation system (PHABSIM) using the habitat suitability indexes (HSI) of three fish species Z. koreanus, C. splendidus and Pseudopungtungia nigra selected as indicator species. In Z. koreanus, HSI for flow velocity and water depth were estimated at 0.1 to 0.4 m/s and 0.2 to 0.4 m, respectively. In P. nigra, HSI for flow velocity, water depth and substrate size were estimated at 0.2 to 0.5 m/s and 0.4 to 0.6 m and fine gravel to cobbles, respectively. OEF values increasing from up to downstream was found to increase, weighted usable area (WUA) values increased accordingly.