• Journal of Ginseng Research
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• 제43권3호
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• pp.361-367
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• 2019

Panax ginseng, known as Koran ginseng, one of the most commonly used traditional plants, has been demonstrated to show a wide range of pharmacological applications. Ginsenosides are the major active ingredients found in ginseng and are responsible for the biological and pharmacological activities, such as antioxidation, antiinflammation, vasorelaxation, and anticancer actions. Existing studies have mostly focused on identifying and purifying single ginsenosides and investigating pharmacological activities and molecular mechanisms in cells and animal models. However, ginsenoside studies based on clinical trials have been very limited. Therefore, this review aimed to discuss the currently available clinical trials on ginsenosides and provide insights and future directions for developing ginsenosides as efficacious and safe drugs for human disease.

• 한국컴퓨터정보학회:학술대회논문집
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• 한국컴퓨터정보학회 2022년도 제66차 하계학술대회논문집 30권2호
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• pp.517-518
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• 2022

본 논문에서는 360도 카메라기반 식물 전자 상거래서비스 어플을 제안한다. 식물을 이용한 재테크 일명 "식테크"라고 불리는 재테크가 증가하고 있는 추세이다. 현재 다른 중고거래 플랫폼에서는 본 거래가 제안되어 있어 거래가 어렵고 일종의 재테크의 개념이라 전문적으로 거래하기 힘들다는 문제점이 있다. 이를 해결하기 위해 본 프로젝트에서는 실시간 차트를 활용하여 식물 거래가를 알려주고, 360도 카메라와 VR을 활용하여 식물의 육성일기 공유하여 어플 사용자들의 커뮤니티 장소가 되고, 은퇴 후 귀농을 하는 사용자에게는 식물을 잘 기르는 법의 정보를 알 수 있는 다방면에서 활용이 가능하여 여러 문제를 효율적으로 해결할 것으로 기대된다.

• 대한약침학회지
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• 제12권3호
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• pp.49-59
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• 2009

Background : Anticancer effects of herbal medicine have been reported in various types of cancer, but the systematic approaches to explain molecular mechanism(s) are not established yet. Objective : The purpose of this study is to investigate the apoptotic cell death by Egg White combined Chalcanthite in NCI-H460 human lung cancer cells. Methods : Inhibitory effects were estimated by the MTT-assay. Cancer cells were stained with DAPI and showed condensed and fragmented nuclei. The expression of cleaved caspase-3, bcl-2, and bax was detected by western blotting. To establish a basis of understanding for anti-cancer mechanism, whole proteins have been obtained from NCI-H460 harvested at 24 hrs after the treatment of Egg White combined Chalcanthite, protein expression has been profiled by 2DE-based proteomic approach. Results : NCI-H460 human lung cancer cells were treated by three samples of IS3, IS4 and IS5. IS4 inhibited most effectively the growth of NCI-H460 human lung cancer cells. The expression of cleaved caspase-3 increased in IS4 in a concentration-dependent manner. Various changes of the protein expression have been monitored, and most frequent dysregulation was found in Vimentin, Lamin-A/C. Conclusion : Egg White combined-Chacanthite inhibited the growth of NCI-H460 human lung cancer cells by inducing the apoptotic cell death via caspase-3 activation. Based upon the present findings, the further study will focus on monitoring various cancer survival factors after artificial regulation of the proteins identified, and it would be the basis for the understanding of the Chacabthite anticancer effect(s) at the molecular level.

• 천문학회보
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• 제34권1호
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• pp.113.1-113.1
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• 2009

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 2009년도 한국우주과학회보 제18권1호
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• pp.54.3-55
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• 2009

• 해부∙생물인류학
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• 제31권4호
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• pp.121-131
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• 2018

사람의 키는 당사자가 갖고 있는 고유한 생물학적 특질이기 때문에 어떤 사람의 신원을 특정하고자 할 때 유용한 단서로 활용할 수 있다. 이러한 이유로 범죄 사건이나 재난 상황이 발생한 경우 신원 불상 피해자의 신원을 확인하기 위해 표준화된 절차에 따라 키를 추정한다. 하지만 키를 추정하는 절차나 방법이 올바르지 않다면 추정된 키의 정확도는 낮을 수밖에 없다. 본 연구에서는 국내에서 발견된 백골 변사자에 대한 법의인류학적 감정 내용 가운데 키 추정을 위해 사용되는 방법의 적정성을 검토하고 키 추정치의 정확도를 높일 수 있는 방법들을 살펴보고자 한다. 이를 위해 국립과학수사연구원에 의뢰된 560건의 백골 변사자에 대한 부검 결과를 검토하였고, 구체적인 논의를 위해 고(故) 유병언씨(이하 '유씨'로 칭함)의 키 추정 사례를 이용하였다. 유씨의 키는 Trotter (1970)의 공식 가운데 표준오차가 더 적은 종아리뼈 공식이 있음에도 불구하고 넙다리뼈 공식을 이용해 추정됐다. 추정 결과를 보고하는데 있어서 '표준오차(standard error)'를 '오차구간'으로 간주함으로써 추정치의 범위를 지나치게 좁게 제시했다. 또, 나이듦에 따른 키 감소분을 고려하지 않았기 때문에 부검감정서 상의 유씨의 키는 사망 당시의 키가 아닌 생전 당시의 최고 키라고 해석하는 게 타당하다. 마지막으로, 한국인 여성의 키를 추정하기 위해 백인 여성 공식을 사용하게 되면 실제보다 키를 작게 추정할 가능성이 높다. 키 추정의 정확도를 높이기 위해 해부학적 방법을 고려할 필요가 있다. 만약 해부학적 방법의 적용이 곤란한 경우라면 한국인 자료를 이용해 개발된 공식을 적용하는 게 바람직하다. 1980년대 이후 한국인 자료를 이용해 키를 추정할 수 있는 방법들이 다수 개발됐다. 한국인을 대상으로 한 공식을 적용해 변사자의 키를 보다 정확히 추정한다면 향후 한국에서 발견된 변사자의 신원 확인 가능성 또한 높아질 것으로 기대된다.

• Biomolecules & Therapeutics
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• 제24권6호
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• pp.595-603
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• 2016

(E)-3-Phenyl-1-(2-pyrrolyl)-2-propenone (PPP) is a pyrrole derivative of chalcone, in which the B-ring of chalcone linked to ${\beta}$-carbon is replaced by pyrrole group. While pyrrole has been studied for possible Src inhibition activity, chalcone, especially the substituents on the B-ring, has shown pharmaceutical, anti-inflammatory, and anti-oxidant properties via inhibition of NF-${\kappa}B$ activity. Our study is aimed to investigate whether this novel synthetic compound retains or enhances the pharmaceutically beneficial activities from the both structures. For this purpose, inflammatory responses of lipopolysaccharide (LPS)-treated RAW264.7 cells were analyzed. Nitric oxide (NO) production, inducible NO synthase (iNOS) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) mRNA expression, and the intracellular inflammatory signaling cascade were measured. Interestingly, PPP strongly inhibited NO release in a dose-dependent manner. To further investigate this anti-inflammatory activity, we identified molecular pathways by immunoblot analyses of nuclear fractions and whole cell lysates prepared from LPS-stimulated RAW264.7 cells with or without PPP pretreatment. The nuclear levels of p50, c-Jun, and c-Fos were significantly inhibited when cells were exposed to PPP. Moreover, according to the luciferase reporter gene assay after cotransfection with either TRIF or MyD88 in HEK293 cells, NF-${\kappa}B$-mediated luciferase activity dose-dependently diminished. Additionally, it was confirmed that PPP dampens the upstream signaling cascade of NF-${\kappa}B$ and AP-1 activation. Thus, PPP inhibited Syk, Src, and TAK1 activities induced by LPS or induced by overexpression of these genes. Therefore, our results suggest that PPP displays anti-inflammatory activity via inhibition of Syk, Src, and TAK1 activity, which may be developed as a novel anti-inflammatory drug.

• BMB Reports
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• 제55권4호
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• pp.198-203
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• 2022

As negative regulators of cytokine signaling pathways, suppressors of cytokine signaling (SOCS) proteins have been reported to possess both pro-tumor and anti-tumor functions. Our recent studies have demonstrated suppressive effects of SOCS1 on epithelial to mesenchymal signaling in colorectal cancer cells in response to fractionated ionizing radiation or oxidative stress. The objective of the present study was to determine the radiosensitizing action of SOCS1 as an anti-tumor mechanism in colorectal cancer cell model. In HCT116 cells exposed to ionizing radiation, SOCS1 over-expression shifted cell cycle arrest from G2/M to G1 and promoted radiation-induced apoptosis in a p53-dependent manner with down-regulation of cyclin B and up-regulation of p21. On the other hand, SOCS1 knock-down resulted in a reduced apoptosis with a decrease in G1 arrest. The regulatory action of SOCS1 on the radiation response was mediated by inhibition of radiation-induced Jak3/STAT3 and Erk activities, thereby blocking G1 to S transition. Radiation-induced early ROS signal was responsible for the activation of Jak3/Erk/STAT3 that led to cell survival response. Our data collectively indicate that SOCS1 can promote radiosensitivity of colorectal cancer cells by counteracting ROS-mediated survival signal, thereby blocking cell cycle progression from G1 to S. The resulting increase in G1 arrest with p53 activation then contributes to the promotion of apoptotic response upon radiation. Thus, induction of SOCS1 expression may increase therapeutic efficacy of radiation in tumors with low SOCS1 levels.