• Title/Summary/Keyword: Yellow rot

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Isolation and characterization of antifungal violacein producing bacterium Collimonas sp. DEC-B5 (항진균활성 violacein 색소를 생산하는 Collimonas sp. DEC-B5 균주의 분리 및 특성)

  • Lee, Ye-Rim;Mitchell, Robert J.;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.52 no.2
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    • pp.212-219
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    • 2016
  • Forty-nine pigments were extracted from the collections of 106 pigment producing bacteria from the plant rhizosphere soil. Antibacterial activity test was performed in the subjects of the extracted pigments with plant pathogenic bacteria including Xanthomonas axonopodis and Xanthomonas campestris, and with plant pathogenic fungi including Botrytis cinerea, Colletotrichum acutatum, and Fusarium oxysporum. The yellow pigment by Chryseobacterium sp. RBR9 and the red pigment by of Methylobacterium sp. RI13 showed the antibacterial activities against Xanthomonas axonopodis and Xanthomonas campestris. The violet pigment by Collimonas sp. DEC-B5 showed the antibacterial activity as well as the antifungal activities against Botrytis cinerea and Fusarium oxysporum. Especially, the violet pigment inhibited the growth of Botrytis cinerea more than 65% at MIC $20{\mu}M$. Upon the HPLC analysis result for the isolation of pigment with antifungal activity, violacein (91.6%) and deoxyviolacein (8.4%) were isolated for the pigment by Collimonas sp. DEC-B5. The production amount of the pigment was increased more than 10 times higher when D-mannitol 1.5% and yeast extract 0.2% were added as the nitrogen source to SCB medium. This study suggests that produced violacein by Collimonas sp. DEC-B5 will be effective to control strawberry gray-mold rot fungi by its preventive activity.

Gui Valley: A High Yielding Potential and Good Processing Potato Cultivar

  • Lim, Hak-Tae;Dhital, Shambhu Prasad;Khu, Don-Man;Li, Kui-Hwa;Choi, Seon-Phil;Kang, Chang-Won;Kim, Tae-Joo;Mo, Hwang-Sung;Hwang, Won-Nam;Lee, Woo-Jong
    • Korean Journal of Plant Resources
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    • v.22 no.6
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    • pp.483-488
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    • 2009
  • The main objective of this breeding program is to develop high yielding, disease resistance and good processing potato cultivar. 'Gui Valley' is a clonal selection resulting from a cross between 'ND2471-8' and 'Cona'. It has medium plant height and light green foliage. 'Gui Valley' has medium flowering habit and light pink flowers. 'Gui Valley' is a medium maturing potato cultivar and tubers are smooth, yellow skin, light yellow flesh, long-oval tuber shape, distinct red eyes with medium depth and medium dormancy. It has high level of tuber uniformity and good keeping quality. 'Gui Valley' demonstrates resistance to potato virus Y (PVY), soft rot, but moderately susceptible to late blight and common scab. It is also resistant to most of the internal and external physiological disorders particularly dehiscence, hollow heart and internal brown spot. The specific gravity of 'Gui Valley' is significantly higher (1.097) than that of 'Shepody' (1.078). 'Gui Valley' has suitable for processing mainly French fries and chips. This cultivar has high level of tuber uniformity and capable of yielding 37.6 $t{\cdot}ha^{-1}$, which is 18.2% higher than the control potato cultivar 'Shepody' under optimum agronomical practices.

Breeding of a New Saxifraga fortunei Cutivar (Hybrid) 'Whitegreen' with Light Green and White Colored Petals as a Pot Flower (밝은 연녹색의 분화용 바위떡풀 신품종 '화이트그린' 육성)

  • Suh, Jong-Taek;Hong, Su-Young;Yoo, Dong-Lim
    • Korean Journal of Plant Resources
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    • v.27 no.5
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    • pp.588-592
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    • 2014
  • A new Saxifraga fortunei cultivar 'Whitegreen' was bred by crossing between 'Daewoojoo' with Pale Green (Y-G145-C) colored petals and 'Kwanglim' with light Yellow green (Y-G145A) colored petals. The selection and flower characterization were conducted from 2004 to 2008 in greenhouse of Highland Agriculture Research Center, NICS, RDA. Flowering in 'Whitegreen' Showed from September 20 th for 33 days. The number of petals were 11.4 ea., with a width of 1.4 cm and length of 1.4 cm. The plant height was 20.7 cm, and number of leaves per plant was 48.3 ea., with a width of 3.4 cm and length of 3.0 cm. 'Whitegreen' cultivar can be used as a pot flower. 'Whitegreen' plants grown under 50% shade of sunlight have higher degree of acclimation compared to those under direct sunlight. And soft rot disease would occur usually by over-irrigation. 'Whitegreen' cultivar was registered as a novel variety with No. 3456 in Korea seed and variety service on April 2011.

Characteristics of Korean ginseng varieties of Gumpoong, Sunun, Sunpoong, Sunone, Cheongsun, and Sunhyang

  • Lee, Jang-Ho;Lee, Joon-Soo;Kwon, Woo-Saeng;Kang, Je-Yong;Lee, Dong-Yun;In, Jun-Gyo;Kim, Yun-Soo;Seo, Jiho;Baeg, In-Ho;Chang, Il-Moo;Grainger, Keith
    • Journal of Ginseng Research
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    • v.39 no.2
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    • pp.94-104
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    • 2015
  • Background: Ginseng (Panax ginseng Meyer) is an important medicinal herbs in Asia. However, ginseng varieties are less developed. Method: To developed ginseng varieties, a pure line selection method was applied in this study. Results: Gumpoong was testing of 4-yr-old specimens in 2002, the proportions of the below-ground roots that were rusty colored for Gumpoong was 1.29 in Daejeon and 1.45 in Eumseong, whereas the proportions for its yellow berry variant were 2.60 and 2.45 in the two regions, respectively. Thus the Gumpoong was resistant to root rust. Sunpoong has a high yielding property. Its average root weight is 70.6 g for 6-yr-old roots. Its yield is 2.9 kg/$1.62m^2$ and the rate of heaven- and earth-grade product is 20.9%, which is very high compared to 9.4% for Yunpoong. Sunone is resistance to root rot and the survival rate of 4-yr-old roots was 44.4% in 1997, whereas that of the violet-stem variant landrace was 21.7%. Sunhyang has content of arginyl-fructosyl-glucose (AFG), which produces the unique scent of red ginseng, is $95.1{\mu}mol/g$ and greater than the $30.8{\mu}mol/g$ of Chunpoong in 6-yr-old plants. Sunun and Cheongsun are being nurtured to protect genetic resources. Conclusion: Developed ginsneg varieties will be used as the basis for the protection of genetic resources and breeding.

Use of hot water, combination of hot water and phosphite, and 1-MCP as post-harvest treatments for passion fruit (Passiflora edulis f. flavicarpa) reduces anthracnose and does not alter fruit quality

  • Dutra, Jaqueline Barbosa;Blum, Luiz Eduardo Bassay;Lopes, Leonardo Ferreira;Cruz, Andre Freire;Uesugi, Carlos Hidemi
    • Horticulture, Environment, and Biotechnology : HEB
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    • v.59 no.6
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    • pp.847-856
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    • 2018
  • This research aimed to evaluate the effectiveness of hot water ($43-53^{\circ}C{\cdot}5min^{-1}$; $47^{\circ}C{\cdot}2-6min^{-1}$), 1-methylcyclopropene (1-MCP) at $50-300nL\;L^{-1}$ and a combination of hot water ($47/49^{\circ}C{\cdot}5min^{-1}$) and phosphite $40%\;P_2O_5+20%\;K_2O$;$40%\;P_2O_5+10%\;Zn$) in anthracnose control and the effect on fruit quality [fresh weight loss (FWL-%); pH, total soluble solids ($TSS-^{\circ}Brix$), and titratable acidity (TA = % citric acid (CA)] of passion fruit ( Passiflora edulis f. flavicarpa ) at the postharvest stage. When the fruits were in the stage of 0% dehydration and fully yellow peels, they were disinfested and inoculated with Colletotrichum gloeosporioides. They were then subjected to the above mentioned treatments; this was followed by incubation for 120 h. The diameter of the disease lesions was monitored daily. After the incubation, a physico-chemical analysis was performed. Hot-water treatment resulted in disease reduction at 47 and $49^{\circ}C$ for 4 and 5 min. The combination of hot-water treatment at $47^{\circ}C$ (4 or 5 min) and application of the phosphite of K or Zn significantly reduced disease severity in fruits. The 1-MCP treatment reduced anthracnose severity in passion fruit mainly at $200nL\;L^{-1}{\cdot} 24h^{-1} $. None of the treatments significantly changed the physico-chemical characteristics of the fruit [FWL (2.6-4.1%); pH (3.2-3.5), TSS ($8.9-10.9^{\circ}Brix$), and TA (1.8-2.5% CA)].

Some Aspects of High Lysine Maize Breeding using Opaque-2 Gene (Opaque-2 인자를 이용한 고라이신 옥수수의 육종)

  • Bong-Ho Chae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.5 no.1
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    • pp.57-64
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    • 1969
  • Several field and sweet corn varietiea from several sources were crossed with a variety carrying the opaque-2 gene to determine the phenotypic interactions in the breeding of high lysine maize. Although opaque-2 lines showed lower protein content than the corresponding normal varieties, there was no correlation between the protein levels of the two types. opaque-2 maize contained more lysine, but no relationship was found between the protein content and the lysine content of either normal or opaque-2 types, suggesting that high lysine corn using the opaque-2 gene may be developed independently from the protein content. The F2 segregation ratios for normal and opaque-2, 100-kernel weights, percentage seed set, opaque-2 phenotype, disease susceptibility, and the relationship between protein and lysine content of normal and opaque-2 were investigated. The determinations and observations were made on the F2, F3, and BC1 Lysine content was determined by the ion exchangeresin combined with paper chromatography method. Most crosses segregated in a 1-opaque-2 : 3-normal ratio as expected. Opaque-2 segregates were lighter than the normal type and smaller in size. A mottled phenotype of opaque-2 maize observed in the Philippines yellow endersperm. In some varieties opaque-2 maize was very susceptible to the ear and kernel rot disease. No. 5(female) and opaque-2(male). Selectlon of a double mutant of waxy and opaque-2 by using the iodine technique and electric lamp was discussed. opaque-2 and floruy-2 were not allels. Different percentage of seed set were observed in the segregation of aewx crossed with opaque-2. An unusual gametophytie relationship was involved in a cross between Glutinous.

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Suppression of Powdery Mildew Using the Water Extract of Xylogone ganodermophthora and Aqueous Potassium Phosphonate Solution on Watermelon under Greenhouse Conditions (Xylogone ganodermophthora 배양체 추출물 및 아인산칼륨 수용액을 이용한 시설수박 흰가루병 발생 억제효과)

  • Kang, Hyo-Jung;Kim, Youngsang;Kim, Taeil;Jeong, Taek Ku;Han, Chong U;Nam, Sang Young;Kim, Ik-Jei
    • Research in Plant Disease
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    • v.21 no.4
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    • pp.309-314
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    • 2015
  • Xylogone ganodermophthora (Xg) is an ascomycetous fungus that causes yellow rot on cultivated Ganoderma lucidum. Previously, we reported in vitro antifungal activities of a Xg culture extract against several watermelon pathogens. In 2014, we conducted greenhouse experiments to evaluate the control efficacy of a water extract of cultured Xg on watermelon powdery mildew (WPM). The test material (stock solution, ca. $4,000{\mu}g/ml$) was prepared by an autoclaved Xg culture in water at a ratio of 800 g of culture per 6 liter of water, and then filtering it through filter paper. Six foliar applications of the solutions (diluted 100- and 1,000-fold) significantly suppressed the formation of conidiophores and conidia. The inhibitory effect of aqueous potassium phosphonate solution on the disease and its phytotoxicity was tested. Phytotoxicity on watermelon plants was observed at concentrations of 1,000 and $2,000{\mu}g/ml$ as irregular brownish spots. The control efficacies against WPM were 91.9% at $2,000{\mu}g/ml$, 64.9% at $1,000{\mu}g/ml$, and 62.2% at $500{\mu}g/ml$.

Breeding of a New Mid-season Apple Cultivar 'Yeohong' (중생종 사과 '여홍(麗紅)' 품종 육성)

  • Kwon, Soon-Il;Kim, Mok-Jong;Paek, Pong Nyeol;Shin, Yong-Uk;Kim, Jung-Hee;Choi, Cheol;Kang, In-Kyu
    • Horticultural Science & Technology
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    • v.30 no.6
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    • pp.776-779
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    • 2012
  • A new cultivar 'Yeohong' was originated from an artificial cross between 'Jonathan' and 'Fuji' carried out at National Institute of Horticultural & Herbal Science in 1993. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2002. After regional adaptability test at five districts for subsequent four years as 'Wonkyo Ga-31', it was finally selected in 2007. 'Yeohong' is characterized by the following. Optimum harvest time is late September. Shape of mature fruit at 278 g on mean weight is oblate a light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains favorable total soluble solids at $14.3^{\circ}Brix$ and titratable acidity at 0.4%, which results in a gustatory harmony between sugars and acids. Safe storage period for the fruit is 3 weeks at room temperature. It is not resistant to Bitter rot. 'Yeohong' also reveals a physiological cross compatibility with leading cultivars such as 'Hongro' and 'Tsugaru', but a physiological cross incompatibility with 'Fuji'. It has weak growth habit.

'Hongan', a New Mid-Season Apple Cultivar (중생종 사과 '홍안(紅顔)' 육성)

  • Kwon, Soon-Il;Kim, Jung-In;Kim, Mok-Jong;Paek, Pong-Nyeol;Shin, Yong-Uk;Hwang, Jung-Hwan;Kang, Sang-Jo;Kim, Dae-Il;Choi, Cheol;Kang, In-Kyu
    • Horticultural Science & Technology
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    • v.29 no.6
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    • pp.655-658
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    • 2011
  • A new cultivar 'Hongan' was originated from an artificial cross between 'Fuji' and 'Jonathan' carried out at National Institute of Horticultural & Herbal Science in 1993. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2002. After regional adaptability test at five districts for subsequent four years as 'Wonkyo Ga-32', it was finally selected in 2006. 'Hongan' is characterized by the following. Optimum harvest time is late September. Shape of the mature fruit at 309 g on mean weight is globosity with a light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains favorable total soluble solids at $13.7^{\circ}Brix$ and an acidity at 0.28%. Safe storage period for the fruit is 3 weeks at room temperature. It is resistant to Bitter rot. 'Hongan' also reveals a physiological cross compatibility with leading cultivars such as 'Gamhong' and 'Tsugaru' in addition to its maternal parent 'Fuji'. Tree topology is semi-spreading with vigorous growth habit.

Ecological and Physiological Characteristics of Armillaria solidipes and A. gallica in Korea (잣뽕나무버섯과 곤봉뽕나무버섯의 생태학적 및 생리학적 특성)

  • Kim, Jin-Gun;Lee, Hwa-Yong;Park, Yong-Woo;Lee, Hee-Su;Koo, Chang-Duck
    • The Korean Journal of Mycology
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    • v.46 no.3
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    • pp.255-269
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    • 2018
  • Armillaria is a white-rot fungus that decomposes organics; it is also a root pathogen that kills trees. Recently, since the forest tending practice, the incidence of Armillaria infections on tree stumps has been increasing. Herein, we have examined the morphological features of Armillaria solidipes and A. gallica collected from 10 areas in Korea, and investigated the activities of the decomposing enzymes cellulase and laccase of these two Armillaria species. A. solidipes was found in eight host tree species including Pinus densiflora, Quercus serrata, and Castanea crenata at the altitude of 107~1,396 m, while A. gallica was found in three host species including P. densiflora and Fraxinus rhynchophylla at the altitude of 119~556 m. The fruiting bodies of A. solidipes are close with lamellae, and have brown to black hairs on the stipe, while those of A. gallica are distant with lamellae and clavate-shaped, and have yellow hairs on the base. The activites of cellulase of the two species were not different, but the activity of A. solidipes laccase was higher ($12.94{\pm}0.4U/mL$) than that of A. gallica laccase ($9.14{\pm}0.3U/mL$).