• Animal cells and systems
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• v.15 no.4
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• pp.259-267
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• 2011

This study was undertaken to produce a $F_{ab}$ fragment of a human monoclonal antibody reactive to oxidized and carbamylated low-density lipoprotein (oxLDL and cLDL) using phage display technology. An analysis of DNA sequences of this $F_{ab}$, termed plaque 15,16-46 $F_{ab}$, revealed that the rearranged $V_H$ was highly mutated. Complementarity-determining regions of the $V_H$ showed a very high R/S ratio and contained many positively charged amino acids. In direct binding and competitive ELISA, the $F_{ab}$ reacted strongly with both MDA-LDL and Cu-oxLDL forms of oxLDL, and also showed high affinity for cLDL. Immunofluorescence and immunohistochemical analyses showed that this $F_{ab}$ positively stained atherosclerotic aortic plaques in $ApoE^{-/-}$ mice as well as those in patients with atherosclerosis. The $F_{ab}$ also showed positive staining in placental decidua from patients with preeclampsia. It is suggested that the plaque 15,16-46 $F_{ab}$ against oxLDL and cLDL might possibly be applicable for developing a diagnostic reagent for both human and rodent animal research to detect and characterize atherosclerotic disease progression in atherosclerotic lesions as well as exploring the pathogenesis of atherogenic diseases such as preeclampsia.

• Korean Institute of Interior Design Journal
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• v.21 no.1
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• pp.11-19
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• 2012

The purpose of this study is to categorize elderly people's behavior using the questionnaire filling up by oneself for 24hours and to analyze the time required according to behavior and space. Also, through analytical researches, we find a way to develop smart home service. In this study, we carry out a questionnaire targeting a number of 20 elderly people. The questionnaire is to survey elderly people's behaviors for 24hours. Questionnaire items consist of three parts : items for checking time required about a daily behavior, items for analyzing a space in daily lives, generalities. This study is analyzed through descriptive statistics. The results of this study were as follows. Firstly, the elderly people's behaviors were eight categories. Among these behavior's categories, the highest portion is the behavior related to Individual maintenance such as sleep, personal hygiene, nap, etc. by 49%. Secondly, the portion of time required in residence based on daily lives is higher than outside of residence by 73%. Lastly, analyzing each space in residence focused on time required of elderly people's behaviors, bed room is the highest portion by 49%, and followed by living room with 29% and kitchen with 15%, and bath room with 7%.

• Journal of Korean Clinical Health Science
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• v.3 no.1
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• pp.268-275
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• 2015

Purpose. Most of halitosis is found by people than by themselves in their life. There are some people who are suffering from oral malodor psychasthenia although they do not have halitosis which may disturb social life by sociophobia. Methods. The subjects of this survey were the dental hygienic college students in Busan city and Gyeongnam province from December 1 to December 20, 2013, we use the 281 parts except for the 19 parts of a bad faith response of 300 parts. Results. As a response to the awareness of the halitosis of college students, "the smell became sometime" was highest in 63.3%, and tooth brushing of halitosis, In according to about awareness of halitosis brushing the number of cases showed a 73.4% increase when you answered "no" in case one day the number of brushing at least three times less than a minute halitosis awareness conducted brushing (p<0.05),in case of less than 1 minute brushing time, this was a statistically significant increase halitosis awareness (p<0.05). Conclusions. This study assessment the association between halitosis awareness and oral care habits was to provide students with the basis for education and counseling halitosis in clinical.

• Journal of the Korean Geotechnical Society
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• v.21 no.1
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• pp.93-103
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• 2005

Both static pile load test with load transfer measuring system and the pile dynamic load test are performed to estimate the skin friction and behavior characteristics of a large drilled shaft. And the numerical modeling of large drilled shaft is performed by applying the FDM program. Since the magnitude of friction resistance depends on the relative displacement between soil and shaft, load and displacement at the arbitrary depth along the large drilled shaft are estimated to analyze the correlation. According to the measuring results of load transfer, unit skin friction along the large drilled shaft was fully mobilized at gravel layer in the middle of shaft and the frictional resistance transmitted to bedrock was relatively small. Also, even for the same drilled shaft, the results of PDA and static load test are different with each other and the difference is discussed.

• Korean Journal of Construction Engineering and Management
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• v.8 no.1 s.35
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• pp.159-168
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• 2007

The importance of informatization of architectural/engineering firms has been addressed since technical problems caused by owner and users$^{\circ}{\O}s$ needs and requirements increased. However, the investment and the human resource support have not been enough, and the effectiveness and efficiency of jnformatization has not been verified and validated, either. Therefore, the objective of this paper is to analyze the effectiveness and efficiency of informatization based on the corporation performance through considering the present status of corporation performance and jnformatization of architectural/engineering firms and analyzing the correlations between them. In addition, this study proposed the performance measurement framework to evaluate the performance and jnformatization level on architectural/engineering firms based on the previous studies.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2008.11a
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• pp.305-310
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• 2008

A lot of changes are required in a management method of a project because the character of construction projects gets bigger and higher gradually. Especially, for the efficiency of the management method the plans of various viewpoints focused on the construction supply chain field that the technical application of Ubiquitous Technologies such as RFID/USN is being advanced actively are presented. Recently, the introduction of the intelligent equipment which is developed for intelligence of construction site requires the new process model in the construction supply chain management on the construction site. Therefore, this study is to propose the intelligent construction supply chain reference model development in order to provide the guide that the numerous materials for construction could easily be applied in the changed process, as mentioned above, by overcoming the limit of the existing construction supply chain process which is proposed as bar codes and RFID/USN based individual material types and reflecting the change factors like the intelligent equipment of construction supply chain management.

• Progress in Medical Physics
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• v.28 no.3
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• pp.106-110
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• 2017

The variable density phantom fabricated with varying the infill values of 3D printer to provide more accurate dose verification of radiation treatments. A total of 20 samples of rectangular shape were fabricated by using the $Finebot^{TM}$ (AnyWorks; Korea) Z420 model ($width{\times}length{\times}height=50mm{\times}50mm{\times}10mm$) varying the infill value from 5% to 100%. The samples were scanned with 1-mm thickness using a Philips Big Bore Brilliance CT Scanner (Philips Medical, Eindhoven, Netherlands). The average Hounsfield Unit (HU) measured by the region of interest (ROI) on the transversal CT images. The average HU and the infill values of the 3D printer measured through the 2D area profile measurement method exhibited a strong linear relationship (adjusted R-square=0.99563) in which the average HU changed from -926.8 to 36.7, while the infill values varied from 5% to 100%. This study showed the feasibility fabricating variable density phantoms using the 3D printer with FDM (Fused Deposition Modeling)-type and PLA (Poly Lactic Acid) materials.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• autumn
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• pp.521-524
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• 2003

Recently various construction management systems are applied for more efficient project management, and through them as-built construction information is collected. However, the accuracy of the collected information shows big differences among projects, since tile collection of tile as-built information is not based on objective manner or criteria and also each management personnel uses difference criteria on this based on individual experiences. This initiates the need for a development of methods and information frameworks for efficient collection and management of as-built information with assurance of data accuracy. Therefor the objective of this study is to suggest a method to determine the task performance measurement unit depending on the characteristics of work types. A prototype system was developed for generating task performance measurement units by utilizing MS ACCESS. The information framework and the system developed in this research is expected to help standardized performance measurements across projects by improving the objectivity and the accuracy of the collected information.

• Natural Product Sciences
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• v.26 no.3
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• pp.191-199
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• 2020

Fucoidan, a natural component of brown seaweed, has various biological activities such as anti-cancer activity, anti-oxidant, and anti-inflammatory against various cancer cells. However, the fucoidan has been implicated in melanoma cells via apoptosis signaling pathway. Therefore, we investigated apoptosis with fucoidan in A2058 human melanoma cells with dose- and time-dependent manners. In our results, A2058 cells viability decreased at relatively short-time and low-concentration through fucoidan. This effects of fucoidan on A2058 cells appeared to be mediated by the induction of apoptosis, as manifested by morphological changes through DNA-binding dye Hoechst 33342 staining. When a dose of 80 ㎍/mL fucoidan was treated, the cells were observed: crescent or ring-like structure, chromatin condensation, and nuclear fragmentation. With the increase at 100 ㎍/mL fucoidan, the cell membrane is intact throughout the total process, including membrane blebbing and loss of membrane integrity as well as increase of sub-G1 DNA. Furthermore, to understand the exact mechanism of fucoidan-treated in A2058 cells, western blotting was performed to detect apoptosis-related protein expression. In this study, Bcl-2 family proteins can be regulated by fucoidan, suggesting that fucoidan-induced apoptosis is modulated by intrinsic pathway. Therefore, expression of Bcl-2 and Bax may result in altered permeability, activating caspase-3 and caspase-9. And the cleaved form of poly ADP-ribose polymerase was detected in fucoidan-treated A2058 cells. These results suggest that A2058 cells are highly sensitive to growth inhibition by fucoidan via apoptosis, as evidenced by activation of extracellular signal-regulated kinases/p38/Bcl-2 family signaling, as well as alteration in caspase-9 and caspase-3.

• The Journal of Engineering Geology
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• v.18 no.1
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• pp.7-16
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• 2008

Limestone area have mostly certain geological defects such as the internal cavities due to melting and fractured zone by external pressures. Especially, in case of constructing grand bridge, the treatment of the limestone cavities area having the geological defects is inevitable. In order to reduce foundation settlement and to reinforce the ground in the limestone cavities area, high pressure jet grouting has been carried out as a countermeasure method. Despite the fact that high pressure jet grouting method has already adopted at a lot of limestone cavities area, but the amount of research and technical data on the high pressure jet grouting have not been accumulated properly so for. Therefore this paper intends to investigate the strength characteristics and deformation characteristics for reinforced limestone cavities area by high pressure jet grouting method. In addition, load carrying capacity obtained by static pile load test with load transfer measuring system is analyzed.