• Title/Summary/Keyword: Yd

Search Result 131, Processing Time 0.024 seconds

Predicting residual moment capacity of thermally insulated RC beams exposed to fire using artificial neural networks

  • Erdem, Hakan
    • Computers and Concrete
    • /
    • v.19 no.6
    • /
    • pp.711-716
    • /
    • 2017
  • This paper presents a method using artificial neural networks (ANNs) to predict the residual moment capacity of thermally insulated reinforced concrete (RC) beams exposed to fire. The use of heat resistant insulation material protects concrete beams against the harmful effects of fire. If it is desired to calculate the residual moment capacity of the beams in this state, the determination of the moment capacity of thermally insulated beams exposed to fire involves several consecutive calculations, which is significantly easier when ANNs are used. Beam width, beam effective depth, fire duration, concrete compressive and steel tensile strength, steel area, thermal conductivity of insulation material can influence behavior of RC beams exposed to high temperatures. In this study, a finite difference method was used to calculate the temperature distribution in a cross section of the beam, and temperature distribution, reduction mechanical properties of concrete and reinforcing steel and moment capacity were calculated using existing relations in literature. Data was generated for 336 beams with different beam width ($b_w$), beam account height (h), fire duration (t), mechanical properties of concrete ($f_{cd}$) and reinforcing steel ($f_{yd}$), steel area ($A_s$), insulation material thermal conductivity (kinsulation). Five input parameters ($b_w$, h, $f_{cd}$, $f_{yd}$, $A_s$ and $k_{insulation}$) were used in the ANN to estimate the moment capacity ($M_r$). The trained model allowed the investigation of the effects on the moment capacity of the insulation material and the results indicated that the use of insulation materials with the smallest value of the thermal conductivities used in calculations is effective in protecting the RC beam against fire.

Development of Control Technology for Acid Mine Drainage by Coating on the Surface of Pyrite using Chemicals (산성광산배수의 발생저감을 위한 황철석 표면의 피막형성 기술개발)

  • Ji, Min-Kyu;Yoon, Hyun-Sik;Ji, Eung-Do;Lee, Woo-Ram;Park, Young-Tae;Yang, Jung-Seok;Jeon, Byong-Hun;Shim, Yon-Sik;Kang, Man-Hee;Choi, Jae-Young
    • Journal of Soil and Groundwater Environment
    • /
    • v.15 no.4
    • /
    • pp.46-52
    • /
    • 2010
  • Acid mine drainage occurs when sulfide minerals are exposed to an oxidizing environment. The objective of this study was to inhibit the oxidation of pyrite by applying various coating agent such as $KH_2PO_4$, MgO and $KMnO_4$ over its surface as an oxidation inhibitors. Experiments were conducted for 8 days to test the feasibility of oxidation inhibitors. The optimal condition of coating agent for standard pyrite and IK mine was the combination of 0.01M $KH_2PO_4$, 0.01M NaOAc and 0.01M NaClO. Otherwise, for YD mine the combination of 0.01M $KMnO_4$, 0.01M NaOAc and 0.01M NaClO. The $SO_4^{2-}$ reduction efficiency of pyrite, IK and YD mine samples was 70, 92 and 84%, respectively. For 8 days, no significant increase of $SO_4^{2-}$ from pyrite sample coated with inhibitor was observed. The pH of solution remains in between 4 to 6 for the reaction conditions.

Evaluation of 1,1-Diphenyl-2-Picrylhydrazyl Radical Scavenging Effect, Cytotoxicity and Tyrosinase Inhibition Activities in 4 Species of Herb Plants (허브 식물 4종의 1,1-Diphenyl-2-Picrylhydrazyl 라디칼 소거능, 세포 독성 및 tyrosinase 저해활성 검정)

  • Park, Hye-Won;Jang, Ka-Hee;Hussain, Mubshar;Lee, Dong-Jin
    • Journal of Applied Biological Chemistry
    • /
    • v.55 no.4
    • /
    • pp.201-205
    • /
    • 2012
  • The present study was conducted to evaluate of the 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) radical scavenging effect, cytotoxicity and tyrosinase inhibition activities using methanol extracts from different parts of four herb plants. The results showed that whole and root extracts of yarrow the highest total polyphenol and flavonoid contents as well as whole of yarrow revealed the highest DPPH radical scavenging effect. In cytotoxicity test against three cancer cell lines, HeLa (uterus), SK-Hep-1 (liver), and YD-15 (oral), the whole extract of feverfew showed the highest toxicity with $IC_{50}$ values of $102.58-138.68{\mu}g/mL$. Also, mallow root extract ($71.24{\mu}g/mL$) exhibited potent tyrosinase inhibitory activity comparable to arbutin ($69.56{\mu}g/mL$), which was used as the control.

An orthotopic nude mouse model of tongue carcinoma (구강암 세포주를 이종이식한 설암의 동소위 누드마우스 모델)

  • Chung, Jae-Seung;Kim, So-Mi;Hwang, Young-Sun;Zhang, Xianlan;Cha, In-Ho
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
    • /
    • v.37 no.6
    • /
    • pp.490-495
    • /
    • 2011
  • Introduction: Development of carcinoma on oral tongue may cause bilateral cervical lymph node metastasis, rapid invasion and growth of the cancer cells due to rich blood supply in muscle tissues. It is not only difficult to develop an animal experimental model, but also to proceed follow-up research after the development of such model as the induction of cancer lead to difficulty in taking nutrition for the experimental animals that often causes early death. Materials and Methods: IIn this study, author have transplanted YD-$10B_{mod}$ cells into nude mouse oral tongues with different cells number ($5{\times}10^4$, $5{\times}10^5$, $5{\times}10^6$ cells/mouse) and observed the development aspect of oral tongue cancers. Results: The cancer developed from orthotopic transplantation of YD-$10B_{mod}$ cells into nude mouse oral tongue show invasion and central necrosis of the tumor, similar to the cancers developed human oral tongue cancer. The difference in tumor size and the time of central necrosis development depending on the number of transplanted tumor cells shows the feasibility of extending the survival period of the nude mouse by limiting the transplanted tumor cells to < $5{\times}10^4$ cells/mouse or under per nude mouse. Conclusion: This nude mouse model could be used effectively in developing effective chemotheray agent and establishing an animal experimental model that can be used to study the mechanism of cervical lymph node metastasis of the oral tongue cancer.

Effect of Resveratrol on Oral Cancer Cell Invasion Induced by Lysophosphatidic Acid

  • Kim, Jin Young;Cho, Kyung Hwa;Lee, Hoi Young
    • Journal of dental hygiene science
    • /
    • v.18 no.3
    • /
    • pp.188-193
    • /
    • 2018
  • The aim of the current study was to demonstrate the potential therapeutic efficacy of resveratrol in oral cancer patients. Lysophosphatidic acid (LPA) intensifies cancer cell invasion and metastasis, whereas resveratrol, a natural polyphenolic compound, possesses antitumor activity, suppressing cell proliferation and progression in various cancer cell lines (ovarian, gastric, oral, pancreatic, colon, and prostate cancer cells). In addition, resveratrol has been identified as an inhibitor of LPA-induced proteolytic enzyme expression and ovarian cancer invasion. Furthermore, resveratrol was shown to inhibit oral cancer cell invasion by downregulating hypoxia-inducible factor $1{\alpha}$ and vascular endothelial growth factor expression. Recently, we demonstrated that LPA is important for the expression of transcription factors TWIST and SLUG during epithelial-mesenchymal transition (EMT) in oral squamous carcinoma cells. In this study, we treated serum-starved cultures of oral squamous carcinoma cell line YD-10B with resveratrol for 24 hours prior to stimulation with LPA. To identify an optimal resveratrol concentration that does not induce apoptosis in oral squamous carcinoma cells, we determined the toxicity of resveratrol in YD-10B cells by assessing their viability using the MTT assay. Another assay was performed using Matrigel-coated cell culture inserts to detect oral cancer cell invasion activity. Immunoblotting was applied for analyzing protein expression of SLUG, TWIST1, E-cadherin, and GAPDH. We demonstrated that resveratrol efficiently inhibited LPA-induced oral cancer cell EMT and invasion by downregulating SLUG and TWIST1 expression. Therefore, resveratrol may potentially reduce oral squamous carcinoma cell invasion and metastasis in oral cancer patients, improving their survival outcomes. In summary, we identified new targets for the development of therapies against oral cancer progression and characterized the therapeutic potential of resveratrol for the treatment of oral cancer patients.

Synthesis of Dodecyl Phenol Novolac Epoxy Resin and Physical Properties of Coatings (Dodecyl phenol novolac 에폭시수지의 합성과 도막물성)

  • Lee, Dong-Chan;Kim, Jin-Wook;Choi, Joong-So
    • Journal of the Korean Applied Science and Technology
    • /
    • v.33 no.4
    • /
    • pp.615-626
    • /
    • 2016
  • In the paper, mild solvent soluble alkyl group modified epoxy resins were prepared via a three-step method; (1) the condensation reaction of dodecyl phenol (DP) and formaldehyde, (2) the crosslinking reaction of dodecyl phnol novolac compound (DPC) and bisphenol A diglycidyl ether, (3) the dodecyl phenol novolac epoxy resins containing fatty acid (DPFA) was prepared by introducing fatty acid to DPC. Equivalent ratios of DP and formaldehyde were 1.25~1.333/1.0. Equivalent ratio of DPC and bisphenol A diglycidyl ether (YD-128) was 1.0/2.0. Reactivity, viscosity, molecular weight, solvent solubility, and physical properties of DPFA were investigated. The result show that as the number of aromatic ring of DPFA increased, viscosity increased and solvent solubility improved. When we test the properties of coatings by blending the synthesized DPFA with a white pigment, DPFAC-5 using triphenylphosphine (TPP) as a ring-open catalyst showed optical performance for drying time, adhesion, hardness, impact resistance, acid resistance and storage stability.

Effect of STAT3 on Lysophosphatidic Acid-Induced Oral Cancer Cell Invasion

  • Song, Zi Hae;Cho, Kyung Hwa;Kim, Jin Young;Lee, Hoi Young
    • Journal of dental hygiene science
    • /
    • v.19 no.2
    • /
    • pp.141-146
    • /
    • 2019
  • Background: Oral cancer has a high incidence worldwide and has been closely associated with smoking, alcohol, and infection by the human papillomavirus. Metastasis is highly important for oral cancer survival. Lysophosphatidic acid (LPA) is a bioactive lipid mediator that promotes various cellular processes, including cell survival, proliferation, metastasis, and invasion. Signal transducer and activator of transcription (STATs) are transcription factors that mediate gene expression. Among the seven types of STATs in mammals, STAT3 is involved in invasion and metastasis of numerous tumors. However, little is known about the role of STAT3 in oral tumor invasion. In the present study, we hypothesized that STAT3 mediates LPA-induced oral cancer invasion. Methods: Immunoblotting was performed to analyze LPA-induced STAT3 activation. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was performed to assess the survival rates of YD-10B cells. STAT3 levels in LPA-treated oral tumor cells were evaluated by performing in vitro invasion assay. Results: To the best of our knowledge, this is the first study to demonstrate that LPA enhances STAT3 phosphorylation in oral cancer. In addition, treatment with WP1066, a selective inhibitor of STAT3, at a concentration that does not cause severe reduction in cell viability, significantly attenuated LPA-induced YD-10B cancer cell invasion. Conclusion: The results suggested that LPA induces oral tumor cells with greater invasive potential via STAT3 activation. Our findings provided important insights into the mechanisms underlying mouth neoplasms.

Porphyra tenera induces apoptosis of oral cancer cells (구강암 세포주에서 김 추출물에 의한 세포자멸사 유도)

  • Kim, Sang Chan;Lee, Jong Rok;Park, Sook Jahr
    • The Korea Journal of Herbology
    • /
    • v.30 no.2
    • /
    • pp.25-30
    • /
    • 2015
  • Objectives : Laver (Porphyra tenera), a red algae species, is one of the most widely consumed edible seaweed in Korea. Laver contains various substances such as essential amino acid, fiber, minerals and polyphenols that benefit human health. In the present study, we prepared ethanol extracts from commercially processed product of Porphyra tenera, and evaluated the growth inhibitory effect against human oral squamous carcinoma YD-10B cells. Methods : Cell viability was measured by MTT assay. Apoptosis was confirmed by TUNEL assay and flow cytometry with the green fluorescent dye FITC annexin V entering apoptotic cells and the red fluorescent dye PI not entering. The expression of the relevant proteins was detected using Western blot. Results : Ethanol extracts of Porphyra tenera (PTE, $50-200{\mu}g/m{\ell}$) caused a significant decrease of cell viability in a dose dependant manner. The cell death occurred as a result of apoptotic process as determined by TUNEL assay and flow cytometric analysis. In line with this observation, decrease in procaspase proteins and increase in cytosolic cytochrome c were observed in cells treated with PTE. In addition, exposure to PTE decreased the expression levels of Bcl-2, and induced PARP cleavage and AIF translocation from mitochondria to nucleus. Conclusions : In conclusion, PTE exerts anti-cancer effects by inducing apoptosis via caspase activation and AIF nuclear translocation in YD-10B cells. These results provide evidence for the possible therapeutic effect of Porphyra tenera in oral cancer cells.

Anti-periodontitic Effects of Weissella cibaria SPM402 and Lactobacillus paracasei SPM412 Isolated from Korean Traditional Foods (한국전통식품에서 분리한 Weissella cibaria SPM402와 Lactobacillus SPM412의 항치주염 효능)

  • So Won Kang;Chae Hyeon Seo;Sungsook Choi
    • Journal of Food Hygiene and Safety
    • /
    • v.39 no.4
    • /
    • pp.343-352
    • /
    • 2024
  • This study aimed to develop probiotics with anti-periodontitic effects to help treat inflammation in the tissues surrounding the teeth. We isolated Weisiella cibaria (W. cibareia) SPM402 and Lactobacillus paracasei (L. paracasei) SPM412 from homemade kimchi and used their cell-free supernatants. At a concentration of 10 mg/mL of L. paracasei SPM412 (LP412) inhibited the formation of Fusobacterium nucleatum (F. nucleatum) biofilm by 95.99±0.73%. In addition, 10 mg/mL of LP412 reduced the RQ value of fimA, an adhesin gene of Porphyromonas gingivalis (P. gingivalis) to 0.08±0.05, and the RQ value of radD, an adhesin gene of F. nucleatum, to 0.08±0.008. When the P. gingivalis outer membrane vehicle (Pg OMV) induced inflammation in YD-38 cells, the RQ value of TNF-α was increased to 36.68±1.85, but was reduced to 4.15±0.37 in the presence of 1 mg/mL of W. cibareia SPM402 (WC402). Similarly, in Pg OMV-induced inflammation in THP-1 cells, the RQ value of IL-1β increased to 2,330.65±204.61 but was reduced to 15.19±4.57 in the presence of 15 mg/mL of WC402. In F. nucleatum-induced inflammation in YD-38 cells, the RQ value of IL-8 increased to 15.10±1.11 and was decreased to 2.67±0.50 in the presence of 1 mg/mL of LP412. In conclusion, W. cibaria SPM402 and L. paracasei SPM412 showed potent anti-inflammatory effects against oral pathogenic bacteria and hold promise as functional probiotics with anti-periodontitic activity.

Bile acids from a Marine Sponge-Associated Fungus Penicillium sp.

  • Pil, Gam Bang;Won, Ho Shik;Shin, Hee Jae
    • Journal of the Korean Magnetic Resonance Society
    • /
    • v.20 no.2
    • /
    • pp.41-45
    • /
    • 2016
  • Chemical investigation of a marine-derived fungus, Penicillium sp. 108YD020, resulted in the discovery of six bile acid derivatives, glycocholic acid (1), glycocholic acid methyl ester (2), cholic acid (3), glycochenodeoxycholic acid (4), glycodeoxycholic acid methyl ester (5), and cholic acid methyl ester (6). The structures of six bile acid derivatives 1-6 were determined by the detailed analysis of 1D, 2D NMR and LC-MS data, along with chemical methods and literature data analysis.