• KSBB Journal
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• v.29 no.5
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• pp.380-384
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• 2014

Inhibition effects of peptide hydrolysates from Astragalus membranaceus Bunge. on the expression of the matrix metalloproteinases (MMPs) in human dermal fibroblasts were evaluated in vitro. Crude peptides were obtained by the hydrolysis of proteins extracted from A. membranaceus. Peptides were purified partially by the basis on the molecular weight using 40% polyacrylamide gel electrophoresis before treatment with human dermal fibroblasts. Basis on the doseeffect experiments, expressions of MMPs including MMP-1, MMP-3, MMP-8, MMP-13 in human dermal fibroblasts were evaluated. Expressions of MMP-1, MMP-3, MMP-8 and MMP-13 were reduced in 43%, 5%, 22% and 57% respectively. The mass spectrometric analysis of partially purified peptides from A. membranaceus, which strongly inhibit expressions of MMPs, indicated that the peptides were composed of molecules below 1500 Da.

• BMB Reports
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• v.42 no.10
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• pp.691-696
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• 2009

The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.

• Journal of KIISE:Software and Applications
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• v.28 no.1
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• pp.14-25
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• 2001

For having various applications, XML is widely used in various fields. But, there have not been proposed a system to integrate and manage XML and its applications together. In this paper, we propose methods to integrate web-based XML applications(SMIL, RDF, WIDL) and for object-oriented modeling of each DTD and document instance. We prppose and system to merge object modeling of XML applications. With the proposed integrating algorithm, we can not only easily analysis various web-based XML applications which is represented by complex tags but also generate object-oriented schema for each document and store it to OODBMS.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.5
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• pp.72-78
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• 2008

The cooling effects of botanical garden in urban are was investigated at Sungkyunwan University Natural Science Campus and nearby urban area during summer (20/06/2008-30/08/2008). Temperature and humidity data were observed, downloaded and analyzed. After observation single-family residential area (TNH) showed the highest air temperature while botanical garden (ARB) did the lowest one. UHI intensity between TNH and ARB was derived and investigated. The average UHI intensity was $1.5^{\circ}C$ while maximum UHI intensity was recorded at 21 : 20 by $2.29^{\circ}C$, and minimum UHI intensity at 09 : 20 by $0.45^{\circ}C$. Overall the average air temperature of botanical garden was lower to surrounding urban area by $0.5-1.5^{\circ}C$. So it was found out the botanical garden contributes to the cooling effect of the surrounding area as an urban cooling island.

• Food Science and Biotechnology
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• v.16 no.5
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• pp.807-811
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• 2007

Diabetes is marked by high glucose levels and is associated with decreased bone mass and increased fracture rates. To determine if [6]-gingerol could influence osteoblast dysfunction induced by 2-deoxy-D-ribose (dRib), osteoblastic MC3T3-E1 cells was treated with dRib and [6]-gingerol and markers of osteoblast function and oxidized protein were examined. [6]-Gingerol ($10^{-7}\;M$) significantly increased the growth of MC3T3-E1 cells in the presence of 30 mM dRib (p<0.05). [6]-Gingerol ($10^{-7}\;M$) caused a significant elevation of alkaline phosphatase (ALP) activity, collagen content, and osteocalcin secretion in the cells. We then examined the effect of [6]-gingerol on the production of osteoprotegerin and protein carbonyl in osteoblasts. Treatment with [6]-gingerol ($10^{-9}$ and $10^{-7}\;M$) increased osteoprotegerin secretion in osteoblastic cells. Moreover, [6]-gingerol ($10^{-9}$ and $10^{-7}\;M$) decreased protein carbonyl contents of osteoblastic MC3T3-E1 cells in the presence of 30 mM dRib. Taken together, these results demonstrate that [6]-gingerol inhibits dRib-induced damage and may be useful in the treatment of diabetes related bone diseases.

• Journal of the Korean Institute of Rural Architecture
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• v.12 no.3
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• pp.35-46
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• 2010

The purpose of this study is to interpretate the Korean traditional Private Garden Pavilion -'byoul-seo(別墅)' in the view point of the architectural methodology - the separation of space in Honam province. It is presumed that nature union is archived through the 'yan-sang(玩賞)' which is ultimate state of knowledge that is base on the premises of opened signification in emancipation from narrow view point in the past and cognition of value. therefore, it is needed to know the unworldly point of space perception about the pavilion territory, furthermore to have conceptual method to distinct pavilion from the world. there are two methods in the distinct concept in this pavilion research: unworldly separation and meditative separation. As a result, the followings are conclusions; There two distinctive methods of planning of pavilion by use of the room and column. There are enclosement, turnaround, division of floor area in the room organization method, and it's modification of modulation, diversification of column height and diversification of column use in different space.

• Biomolecules & Therapeutics
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• v.22 no.4
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• pp.334-340
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• 2014

In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-${\kappa}B$ inhibitory activity of the isolated compounds was measured in $TNF{\alpha}$-treated HepG2 cells using a luciferase reporter system. Compounds 19-23 inhibited $TNF{\alpha}$-stimulated NF-${\kappa}B$ activation in a dose-dependent manner, with $IC_{50}$ values ranging from $0.75-8.30{\mu}M$. Compounds 19 and 20 also inhibited the $TNF{\alpha}$-induced expression of iNOS and ICAM-1 mRNA. Moreover, effect of the isolated compounds on PPARs transcriptional activity was assessed. Compounds 7-11 and 19-23 activated PPARs the transcriptional activity significantly in a dose-dependent manner, with $EC_{50}$ values ranging from $0.9-10.8{\mu}M$. These results suggest the presence of potent anti-inflammatory components in P. koreana, and will facilitate the development of novel anti-inflammatory agents.

• Journal of Acupuncture Research
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• v.20 no.1
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• pp.228-235
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• 2003

Objective : Psoriasis is a common skin disease and the difficulty of its medical management is well known. This study was designed to investigate the effect of bee venom aqua-acupuncture on psoriasis. Methods : Bee venom aqua-acupuncture was performed two times a week. The following points were selected: LI-11(曲池), LI-15, K-9(築賓) on both sides of body. For herbal medicine, YanHyulJiHwangTang-gami was given. We checked involvement in plaque, erythematous popules and invasiveness according to psoriasis area severity index(PASI). Results :After 9 weeks of treatment, the patient showed that itching symptoms was disappeared and score of PASI changed from 26.2 to 0. Conclusions : These results suggest that bee venom aqua-acupuncture and herbal medicine has good effect on psoriasis. But further studies are required to concretely prove the effectiveness of bee venom aqua-acupuncture for treating psoriasis.

• Herbal Formula Science
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• v.17 no.2
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• pp.143-150
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• 2009

The effects of aqueous extract of Schizandrae Fructus (AESC) on lead (Pb)-induced changes of monoamine neurotransmitters in the hippocampus (HIP) of adult rats were investigated. Male Sprague-Dawley rats were received intraperitoneal (i.p.) administration of Pb acetate (5 mg/kg/d) for 28 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in HIP were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Pb treated rats (P < 0.05), while pretreatment with AESC (100 mg/kg/d or 300 mg/kg/d, p.o., 2 h before Pb) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC (300 mg/kg/d) significantly increased the reduction of glutathione contents and superoxide dismutase activities in HIP induced by chronic Pb. These results suggest that AESC ameliorates Pb-induced depletion of monoamine neurotransmitters in HIP through its antioxidant activity.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.1
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• pp.44-51
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• 2009

In order to determine air temperature difference by canopy layer in the forest, air temperatures were observed at Seolleung Park, Gahngnam-ku, Seoul. from November 9, 2007 to November 8, 2008 by 10 minute interval. The data were analyzed in terms of diurnal variation based on annual and monthly temperature difference. Using calm, less cloudy and no rainy weather data, average air temperature difference between forest and grass was observed as $0.8^{\circ}C$. The maximum air temperature difference was observed at 22:10, 23:20, 23:30 and 23:40 by $2.13^{\circ}C$ and the minimum one observed at 13:00 by $-0.84^{\circ}C$ in diurnal variation. The maximum temperature difference occurred at 19 : 50 on September by $3.67^{\circ}C$, Overall the air temperature in the forest was higher than that of grass at night and lower in midday.