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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Peptide Hydrolysates from Astragalus membranaceus Bunge Inhibit the Expression of Matrix Metalloproteinases in Human Dermal Fibroblasts

  • Park, Sun Ki (CIR Center, Cosmecca Korea) ;
  • Van Hien, Pham (Cosmetic Science Major, Department of Applied Biotechnology, Graduate School, Ajou University) ;
  • Van Luong, Hoang (Vietnam Military Medical University) ;
  • Yan, Shao-Wei (Cosmetic Science Major, Department of Applied Biotechnology, Graduate School, Ajou University) ;
  • Byun, Sang Yo (Cosmetic Science Major, Department of Applied Biotechnology, Graduate School, Ajou University)
  • Received : 2014.07.26
  • Accepted : 2014.10.24
  • Published : 2014.10.30
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Abstract

Inhibition effects of peptide hydrolysates from Astragalus membranaceus Bunge. on the expression of the matrix metalloproteinases (MMPs) in human dermal fibroblasts were evaluated in vitro. Crude peptides were obtained by the hydrolysis of proteins extracted from A. membranaceus. Peptides were purified partially by the basis on the molecular weight using 40% polyacrylamide gel electrophoresis before treatment with human dermal fibroblasts. Basis on the doseeffect experiments, expressions of MMPs including MMP-1, MMP-3, MMP-8, MMP-13 in human dermal fibroblasts were evaluated. Expressions of MMP-1, MMP-3, MMP-8 and MMP-13 were reduced in 43%, 5%, 22% and 57% respectively. The mass spectrometric analysis of partially purified peptides from A. membranaceus, which strongly inhibit expressions of MMPs, indicated that the peptides were composed of molecules below 1500 Da.

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References

  1. Calleja-Agius, J. and M. Brincat (2013) Skin connective tissue and ageing. Best Practice & Research Clinical Obstetrics and Gynaecology 27: 727-740. https://doi.org/10.1016/j.bpobgyn.2013.06.004
  2. Drugsite Trust, Astragalus. http://www.drugs.com/npc/astragalus. html (2012).
  3. Kitagawa, I., H. Wang, A. Takagi, et al (1983) Chemical constituents of Astragali radix, the root of Astragalus membranaceus Bunge. Cycloastragenol, the 9,19-cyclolanostane-type aglycone astragalosides, and the artifact aglycone astrgenol. Chem. Pharm. Bull. 31: 689. https://doi.org/10.1248/cpb.31.689
  4. Kitagawa, I., H. Wang, M. Saito, et al (1983) Chemical constituents of Astragali radix, the root of Astragalus membranaceus bunge. Astragalosides, I, II, IV, acetylastragaloside I and isoastragalosides I and II. Chem. Pharm. Bull. 31: 698-708. https://doi.org/10.1248/cpb.31.698
  5. Kitagawa, I., H. Wang, M. Saito, and M. Yoshikawa (1983) Chemical constituents of Astragali radix, the root of Astragalus membranaceus bunge. Astragalosides III, V, and VI. Chem. Pharm. Bull. 31: 709-715. https://doi.org/10.1248/cpb.31.709
  6. He, Z. and J. Findlay (1991) Constituents of Astragalus membranaceus. J. Nat. Prod. 54: 810-815. https://doi.org/10.1021/np50075a009
  7. Kitagawa, I., H. Wang, and M. Yoshikawa (1983) Chemical constituents of Astragali radix, the root of Astragalus membranaceus bunge. Astragalosides VII and VIII. Chem. Pharm. Bull. 31: 716-722. https://doi.org/10.1248/cpb.31.716
  8. Li, R., W. C. Chen, W. P. Wang, W. Y. Tian, and X. G. Zhang (2010) Antioxidant activity of Astragalus polysaccharides and antitumor activity of the polysaccharides and siRNA. Carbohydrate Polymers 82: 240-244. https://doi.org/10.1016/j.carbpol.2010.02.048
  9. Lin, L. Z., X. G. He, M. Lindenmaier, et al (2000) Liquid chromatography- electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A. membranaceus. J. Chromatogr. A. 876:87-95 https://doi.org/10.1016/S0021-9673(00)00149-7
  10. Shardella, D., G. F. Fasciglione, M. Gioia, C. Ciaccio, G. R. Tundo, S. Marini, and M. Coletta (2012) Human matrix metalloproteinases: An ubiquitarian class of enzymes involved in several pathological processes. Mole- cular Aspects of Medicine 33: 119-208. https://doi.org/10.1016/j.mam.2011.10.015
  11. Wang, B., Y. D. Gong, Z. R. Li, D. Yu, C. F. Chi, Jian, and Y. Ma (2014) Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound. Journal of Functional Foods 6: 176-185. https://doi.org/10.1016/j.jff.2013.10.004
  12. Fling, S. P. and D. S. Gregerson (1986) Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea. Analytical Biochem. 155: 83-88. https://doi.org/10.1016/0003-2697(86)90228-9
  13. Shagger, H. and G. V. Jagow (1987) Tricine-sodium dodecyl sulfate- polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochem. 166: 368-379. https://doi.org/10.1016/0003-2697(87)90587-2
  14. Peterson, J. T. (2004) Matrix metalloproteinase inhibitor development and the remodeling of drug discovery. Heart Fail Rev. 9: 63-79. https://doi.org/10.1023/B:HREV.0000011395.11179.af
  15. Lauer-Fields, J. L., D. Juska, and G. B. Fields (2002) Matrix metalloproteinases and collagen catabolism. Biopolymers 66: 19-32. https://doi.org/10.1002/bip.10201
  16. Holmbeck, K., P. Bianco, S. Yamada, and H. Birkedal-Hansen (2004) MT1-MMP: a tethered collagenase. J. Cell. Physiol. 200: 11-19. https://doi.org/10.1002/jcp.20065
  17. Quan, T. H., Z. P. Qin, W. Xia, Y. Shao, J. J. Voorhees, and G. J. Fisher (2009) Matrix-degrading metaloproteinases in photoaging. J. Investig. Dermatol. Symp. Proc. 14: 20-24. https://doi.org/10.1038/jidsymp.2009.8
  18. Jenkins, G. (2002) Molecular mechanisms of skin ageing. Mechanisms of Ageing and Development 123: 801-810. https://doi.org/10.1016/S0047-6374(01)00425-0
  19. Rittie, L. and G. J. Fisher (2002) UV-light-induced signal cascades and skin aging. Ageing Research Reviews. 1: 705-720. https://doi.org/10.1016/S1568-1637(02)00024-7
  20. Benson, H. A. and S. Namjoshi (2008) Proteins and peptides: Strategies for delivery to and across the skin. Journal of Pharmaceutical Sciences 97: 3591-3610. https://doi.org/10.1002/jps.21277
  21. Zhang, S., Y. Qiu, and Y. Gao (2014) Enhanced delivery of hydrophilic peptides in vitro by transdermal microneedle pretreatment. Acta Pharmaceutica Sinica B 4: 100-104. https://doi.org/10.1016/j.apsb.2013.12.011