• 한국식품위생안전성학회지
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• 제30권1호
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• pp.120-125
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• 2015

본 연구는 Sprague-Dawley 계통의 4주령 랫드에서 MSG의 반복경구투여 독성평가와 행동실험을 통해 어린랫드의 인지능력과 불안감에 미치는 영향을 평가하기 위해서 시행하였다. 시험군은 MSG 대신 distilled water를 투여한 대조군(Control, n = 4), MSG를 3 g/kg을 투여한 군(Low, n = 4), MSG를 5 g/kg을 투여한 군(High, n = 4)으로 나누어 4주 동안 주5회 경구투여를 하였다. MSG의 안정성을 확인하기 위해 다음과 같은 관찰 및 검사를 하였다. 검사항목으로는 체중의 변화, 임상증상, 행동실험인 T-maze, Elevated-plus-maze와 혈액학적 검사, 혈청생화학적 검사, 병리조직학적 검사를 관찰한 결과 모든 투여군 및 대조군에서 특이할 만한 임상증상과 체중변화는 관찰되지 않았으며, 폐사 및 빈사 동물은 시험 전 기간을 통하여 발견되지 않았다. 행동실험인 T-maze 결과 MSG 고용량 투여군에서 움직이는 횟수가 감소하다 증가하는 양상을 보였고, Elevated-plus-maze 실험에서는 MSG 고용량 투여군의 Open arm의 출입빈도가 증가하는 등 유의적인 변화가 나타났다. 혈액학적 검사 및 혈청생화학적 검사에서는 대조군과 비교 시 유의적인 변화가 관찰되었지만 그 수치가 정상범위 안에 포함되기 때문에 이상을 나타내지 않았다. 병리조직검사 또한 약간의 염증발생 소견이 나왔지만 정상범위 안에 포함되기 때문에 이상을 나타내지 않았다고 판단했다. 따라서, 어린랫드를 이용한 MSG의 반복투여 독성시험 결과 특이할만한 신체적인 변화는 나타나지 않았으나 고용량의 MSG 투여는 어린랫드에서 인지능력의 저하 및 행동의 불안을 유발할 가능성이 있다고 사료된다.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제5권4호
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• pp.333-338
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• 2005

This paper presents the target object search algorithm under Dynamic Programming (DP) in the Tree-type maze. We organized an experimental environment with the concatenation of Y-shape diverged way, small mobile robot, and a target object. By the principle of optimality, the backbone of DP, an agent recognizes that a given whole problem can be solved whether the values of the best solution of certain ancillary problem can be determined according to the principle of optimality. In experiment, we used two different control algorithms: a left-handed method and DP. Finally we verified the efficiency of DP in the practical application using our real robot.

• 한국주거학회논문집
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• 제16권3호
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• pp.109-117
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• 2005

The purpose of this study is to suggest a basic data of playground design that can arouse various curiosities and interests of children. The 13play areas 'A' kindergartem's playground that parent, music, play equipment, soccer field, water, sand, tunnel, maze, animal, plant, ground hill, bike road, and inner court were evaluated by 16 teachers and 165 children between the age of 4 and 5. POE data of teachers were collected by questionnaire. And children chose and rated very fun and interesting 5 play areas of 13 ones based on the photos. The results were as following. 1) 13 play areas were generally used evenly with diverse purpose. 2) the environment of the play areas was deemed very appropriate. 3) maze area and play equipment area were recognized as the most favored play areas by children. 4) teachers recommended soccer field, plants, maze, tunnel, music area as the most favorable play areas.

• 전자공학회논문지A
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• 제32A권1호
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• pp.222-233
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• 1995

For effective module generation, a detailed router which can handle complicated routing regions on multimple layers of interconnection under various constraints is necessary. In this paper, a new improved maze routing technique is described, which can find the shortest path for each net without allowing stacked vias. In this method, two cost values are stored at each grid point. The cost values represent the routing costs from the terminal of the net being routed to the grid point. One cost value shows the cost of the shortest path without making a via at the grid point and the other cost value shows that with making a via at the grid point. This is the first systematic maze routing technique which can find the shortest path without via-stacking.

• 전자공학회논문지A
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• 제30A권12호
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• pp.135-146
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• 1993

A ner general multi-layer area router, called DINOSAUR, is presented in this paper. DINOSAUR can route various types of routing areas, such as L-shaped channel, switchbox with/without obstacles, and rectilinear area with/without internal modules/terminals. The DINOSAUR algorithm consists of three major stages: layerless maze routing, layering by coloring, and rip-up and reroute. In layerless maze roution stage, the route of each net is determined by modified maze algorithm without taking the conflicts(short. circuits) into account. In layering by coloring stage, the layer of each net is determinde by a heuristic coloring algorithm. When the conflicts are not removed, rip-up and reroute process is invoded. In rip-up and reroute stage, the conflicts are removed iteratively. Many test cases have been run, and on all the benchmark data known in the literature DINOSAUR has performed either better than or comparable to the other routers.

• 대한한방소아과학회지
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• 제14권1호
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• pp.9-38
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• 2000

It has been widely said in Korea that early administrations of Cervi cornu parvum (deer antler) to febrile infants affect brain functions. Traditional Oriental Medicine states that the head is easily affected by fever and only an excess of heat causes headaches. Traditional Oriental Medicine also states that Cervi cornu parvum cannot be used in febrile conditions. With the aim of investigating different febrile response to LPS, experiments using intravenous injection of LPS have been carried out on Cervi comu parvum(CCP) and Soahbohyul - tang combined with Cervi comu parvum(SB-CCP) administered rabbits. Experiments were also conducted to evaluate the effects of early administration of CCP on learning and memory in 3 week old rats with LPS fever. These were evaluated by using the Morris water maze and the radial arm maze. Changes in body weight were also observed during this period. The results of these experiments are as follows. 1. In the experiments with febrile rabbits, the CCP and SB-CCP administered group showed statistically significant reductions of fever (p<0.05). 2. In the experiments with febrile rabbits, CCP and SB-CCP administered rabbits resulted in the tendency of lower body temperatures and shorter fever periods than the control group. 3. There were no differences of mean body weight and fever patterns among the 4 groups in the experiments on young rats with LPS fever. 4. There was no statistical difference of mean response latencies among the rats in Group I (DDW administered), GroupIII (CCP administered), and groupIV (SB-CCP administered) in the Morris water maze. However, Group Ⅱ (the scopolamine administered group) showed delayed latencies on the second day of the first session (p<0.05), and the second and third day of the second session (p< 0.05). 5. There were no statistical differences of mean response latencies among the rats in Group I, III and Ⅳ in the radial arm maze, but Group Ⅱ showed delayed latencies on the first and third day of the first session (p<0.05). 6. There was no influence from the administration of CCP and SB-CCP on the general behavior of the rats in Irwin´s test. These results suggest that Cervi cornu parvum and Soahbohyul - tang combined with Cervi comu parvum have anti-pyretic actions on LPS fever. The results also suggest that these drugs have no influence on learning and memory in young rats with LPS fever in the Morris water maze and the radial arm maze.

• 한국식품저장유통학회지
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• 제19권5호
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• pp.767-773
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• 2012

괴각 추출물의 항불안 효능을 탐색하기 위하여 elevated plus-maze, horizontal wire test 및 open field test와 같은 동물행동실험을 통하여 비교한 결과 다음과 같은 결론을 얻었다. Elevated plus-maze를 이용한 본 연구에서 괴각 추출물의 100 mg/kg, 200 mg/kg 및 400 mg/kg 투여군에서 대조군에 비하여 open arm에서 머무른 시간의 백분율이 증가하였고, open arm으로의 출입 백분율 또한 증가하였다. Elevated plus-maze를 이용한 길항실험에서는 benzodiazepine 수용체의 antagonist인 flumazenil에 의해 괴각 추출물 400 mg/kg의 항불안 효능이 차단되는 것이 관찰되었다. Locomotor activity 측정에서도 괴각 추출물의 모든 용량에서 총 이동거리의 변화가 없었으며, 또한 horizontal wire test에서도 대조군과 괴각 추출물 투여군 사이에 차이를 나타내지 않았다. 결론적으로, 본 연구의 결과에서 괴각의 메탄올 추출물이 elevated plus-maze test, horizontal wire test 및 open field test를 통하여 locomotor activity 및 근육이완이나 진정 등의 부작용이 없으면서 우수한 항불안 작용을 가지는 천연물이라고 생각되어지며 이러한 작용이 특히 GABA 신경계와 관련이 있음을 시사하고 있다. 향후 괴각의 항불안 작용의 평가를 위하여 다양한 실험모델의 개발이 필요할 것으로 생각되며 또한 이러한 작용에 대한 기전 연구가 포괄적이고 다각적으로 진행되어져야 할 필요성이 있다고 사료된다.

• Journal of Applied Biological Chemistry
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• 제58권2호
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• pp.101-104
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• 2015

긴털가루응애 성충을 청색(470 nm), 녹색(520 nm), 황색(590 nm)과 적색(625 nm) light-emitting diode (LED)에서 파장별 광량을 20, 40 및 60 lx로 달리하여 주광성 행동반응 판별장치(LED-equipped Y-maze chamber)에서 상업적 양성대조구 BLB와 유인활성을 비교하였다. 긴털가루응애는 사용된 청색, 녹색, 황색 및 적색 LED 파장과 20, 40 및 60 lx 광량에서 특별한 행동반응특성을 발견하지 못하였으며, 양성대조구로 이용된 BLB에서도 긴털가루응애는 음성/양성주광성 반응을 보이지 않았다. 이러한 결과를 근거로 긴털가루응애는 LED 주광성 행동반응 판별장치에서는 친환경적 해충방제 가능성을 제시하지 못했다.

• 동의신경정신과학회지
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• 제21권2호
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• pp.61-73
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• 2010

Objectives : The purpose of this study was to assess anti-depressive effects of Bee Venom(BV) on an Animal Model of Depression induced immobility stress. Methods : There was 2 pre-experiments MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and Western blot test and 3 main experiments ; forced swimming test, tail suspension test and Y-maze task. Male rats were used for main experiment. The subject was divided into 4 groups(1. control group injected only saline, without immobility stress 2. Negative group injected saline after 2 hours immobility stress 3. Positive group injected Amitriptyline after 2 hours immobility stress 4. BV group injected Bee Venom after 2 hours immobility stress). Each group consisted of 6 rats. Forced swimming test, tail suspension test, Y-maze task were used to evaluate anti-depressive effect of Bee Venom. Results : In MTT assay, as the density of BV increased, the existence rate of primary neuronal cell increased. In Western blot test, the density of CREB and AKT was increasing as time went by. In forced swimming test, BV group showed immobility decreased more than Normal group and Positive group. In tail suspension test, Normal group and Positive group showed immobility decreased more than BV group. In Y-maze task, BV group showed immobility decreased more than Normal group, but Positive group showed immobility decreased more than BV group. Conclusions : These results suggest that Bee Venom may have anti-depressive effect on depression.

• 동의신경정신과학회지
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• 제21권1호
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• pp.125-144
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• 2010

Objectives: The purpose of this study was to characterize the effect of the fraction of Korean mountain ginseng folium (FKG) on the learning and memory impairments induced by scopolamine. Methods: The memory ameliorating effect of FKG was investigated using a passive avoidance test, the Y-maze test, and the Morris water maze test in mice. Drug-induced amnesia was induced by treating animals with scopolamine(1mg/kg, i.p.). Results: FKG (2 or 4mg/kg, p.o.) administration significantly reversed scopolamine-induced cognitive impairments in mice by the passive avoidance test and the Y-maze test(P<0.05), and also improved escape latency in the Morris water maze test at 2 or 4mg/kg(P<0.05). Although FKG has little inhibitory activity for AChE (IC50 value; 1847 ${\mu}g/ml$) in an invitro study, phosphorylated extracellular signal-regulated kinase(pERK) was increased by the administration of FKG inhippocampus on immunohistochemistry. Conclusions: These results suggest that FKG may be a useful cognitive impairment treatment, and its beneficial effects are mediated, in part, via activation of ERK pathway.