• 한국전자파학회논문지
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• 제18권9호
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• pp.1064-1069
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• 2007

본 논문에서는 MMIC 응용을 위한 초소형화된 능동형 $90^{\circ} 전력 결합기를 제안한다. 기존의 수동형 $90^{\circ} 전력 결합기는 매우 큰 크기 때문에 MMIC 상에 집적되어질 수가 없다. 따라서 초소형화된 $90^{\circ} 전력 결합기는 고집적된 MMIC의 개발을 위해 요구되어진다. 본 논문을 위해서 InGaP/GaAs HBT를 이용한 초소형화된 능동형 $90^{\circ} 전력 결합기는 고집적된 MMIC 응용을 위해 GaAs 기판 상에 제작하였다. 제작된 능동형 $90^{\circ} 전력 결합기의 크기는 $2.42{\times}1.05$ mm이며, 이는 기존의 수동형 $90^{\circ} 전력 결합기의 약 2.2 % 정도의 크기였다. 제작된 능동형 $90^{\circ} 전력 결합기는 2.4 GHz에서 기존의 수동형 $90^{\circ} 전력 결합기보다 약 10 dB의 이득 특성과 $-92.6^{\circ}의 양호한 위상차 결합 특성을 보였다. 초소형화된 능동형 $90^{\circ} 전력 결합기는 기존의 수동형 $90^{\circ} 전력 결합기들에 비해서 좋은 RF 성능을 나타내었다. 본 논문은 능동형 $90^{\circ} 전력 결합기에 대한 최초의 보고이다.

• 생명과학회지
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• 제26권7호
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• pp.826-834
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• 2016

본 연구는 Hsp90 inhibitor 및 SIRT1 inhibitor의 병용처리가 항암제 다제내성(MDR) 인간 암세포의 증식 억제에 효과적임을 밝혔다. SIRT1 활성 억제가 Hsp90 inhibitor인 17-AAG의 세포 독성의 효과를 증강시켰으며, 이로 인해 Hsp90 inhibitors에 대한 내성을 극복시킬 수 있음을 인간 자궁암세포인 HeyA8의 MDR 변이주인 HeyA8- MDR 세포에서 확인하였다. SIRT1 inhibitor는 Hsp90 inhibitor에 의한 Hsp90 샤페론 기능 억제를 증강시키며, ubiquitin ligase CHIP의 발현 증강을 유발하여, Hsp90 client protein 인 mutant p53 (mut p53)의 분해를 촉진시킨다. Mut p53 의 발현 감소는 암세포의 Hsp90 inhibitor 내성 획득의 가장 중요한 원인으로 지적되는 heat shock factor 1 (HSF1)/heat shock proteins (Hsps)의 발현 억제와 관련됨을 알 수 있었으며, 이는 항암제 다제내성 세포에서 SIRT1 inhibitor에 의하여 Hsp90 inhibitor에 대한 감수성이 증강되는 분자적 기전임을 밝혔다. 그러므로, SIRT1 억제에 의한 mut p53/HSF1 발현 감소가 MDR 암세포의 Hsp90 inhibitors 내성 극복에 매우 유효함을 시사하는 결과를 얻었다.

• 대한화학회지
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• 제13권2호
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• pp.181-186
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• 1969

The concentration of Strontium-90 in liquid whole milk produced in Korea were measured during past four years. The samples of milk were purchased from dairies in Seoul. Strontium-90 was analysed radiochemically and the amount of stable calcium was also determined. Radioactivity of Yttrium-90 was counted in low background beta counter which has the background of 1.38 cpm. The concentrations of Strontium-90 in the milk are 25.1 PCi $^{90}Sr$/g.Ca in 1965, 26.8 PCi $^{90}Sr$/g. Ca in 1966, 13.7 PCi 90Sr/g.Ca in 1967 and 18.2 PCi $^{90}Sr$/g.Ca in 1968 in annual average. The concentrations of Strontium-90 in the milk of 1967 and of 1968 were decreased approximately compared with the values of 60% 1965 and 1966. From the results we can see that Strontium-90 concentrations in the milk vary roughly proportionally with the specific activity of fallout. Considering on the safety problems, the Strontium-90 levels in the milk produced in Korea were far below the maximum permissible level recommended by ICRP.

• IMMUNE NETWORK
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• 제10권6호
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• pp.206-211
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• 2010

Background: Dendritic cell (DC)-based tumor vaccine is an attractive modality for the treatment of colon cancer because it has been recurred and produced few side effects in patients. Secretory glycoprotein 90K has been found at elevated level in various cancer tissues and sera. We investigated to establish a more effective DC vaccine for the treatment of colon cancer in which the levels of 90K are elevated. Methods: We obtained the concentrated 90K from 293T cells stably expressing 90K. DCs were cultured from peripheral blood monocytes, and a DC vaccine pulsed with tumor lysate was compared with a DC vaccine pulsed with 90K. We measured the functional activity for CTLs by using IFN-${\gamma}$-enzyme linked immunoabsorbent spot (ELISPOT) assay. Results: DCs pulsed with tumor lysate+90K exhibited the enhanced T cell stimulation, polarization of $\ddot{i}$ T cell toward Th1. The CTLs generated by DCs pulsed with 90K efficiently lysed HCT116 cells. The results indicate that 90K-speicifc-CTLs can recognize 90K proteins naturally presented by colon cancer cells. Conclusion: Our study suggests that 90K-specific CTLs generated by 90K-pulsed DCs could be useful effector cells for immunotherapy in colon cancer.

• 한국안광학회지
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• 제5권2호
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• pp.99-105
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• 2000

작업환경에서 시력저하가 나타나는 굴절이상의 종류별 분포는 안경사 100명, 사진기사 100명, 대학생 100명중 단성근시 35% 근시성 단난시 2% 근시성 복난시 30.6% 혼합난시 6.26% 원시성 복난시 3.74% 정시 22.4%의 순으로 나타났다. 양안 굴절이상의 근시도수 분포는 -0.50~-2.00 Dptr가 전체 대상자중 14%, -2.00~-6.00 Dptr 이상이 16% -6.00 Dptr 이상이 5%로 나타났으며, 근시성 단난시의 분포는 C-0.25(90, 180)~C-2.00 Dptr Axis(90, 180) 2%, 근시성 복난시의 분포는 S-0.25 C-0.25 Dptr Axis(90, 180)~S-1.00 C-1.00 Dptr Axis(90, 180)이 22.6%, S-1.00 C-1.00 Dptr Axis(90, 180)~S-2.00 C-2.00 Dptr Axis(90, 180)이 8%로 나타났다. 혼합난시의 분포는 S+0.25 C-0.25 Axis(90, 180)~S+2.00 C-2.00 Axis(90, 180)가 6.26%, 원시성 복난시의 분포는 S+0.25 C+0.25 Axis(90, 180)~S+1.00 Dptr Axis(90, 180)가 3.4%, S+1.00 C+1.00 Axis(90, 180)~S+2.00 C+2.00 Dptr Axis(90, 180)가 0.34%의 순으로 나타났다.

• Biomolecules & Therapeutics
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• 제24권5호
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• pp.453-468
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• 2016

There is a conserved ATPase domain in topoisomerase II (topo II) and heat shock protein 90 (Hsp90) which belong to the GHKL (gyrase, Hsp90, histidine kinase, and MutL) family. The inhibitors that target each of topo II and Hsp90 are intensively studied as anti-cancer drugs since they play very important roles in cell proliferation and survival. Therefore the development of dual targeting anti-cancer drugs for topo II and Hsp90 is suggested to be a promising area. The topo II and Hsp90 inhibitors, known to bind to their ATP binding site, were searched. All the inhibitors investigated were docked to both topo II and Hsp90. Four candidate compounds as possible dual inhibitors were selected by analyzing the molecular docking study. The pharmacophore model of dual inhibitors for topo II and Hsp90 were generated and the design of novel dual inhibitor was proposed.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2004년도 International Meeting of the Microbiological Society of Korea
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• pp.141-146
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• 2004

SPIN90 was identified to farm molecular complex with $\betaPIX$, WASP and Nck. This complex shows that SPIN90 interacts with Nck in a manner dependent upon cell adhesion to extracellular matrix, but $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex was stable even in suspended cells. This suggests that SPIN90 serves as an adaptor molecule to recruit other proteins to Nck at focal adhesions. SPIN90 was phosphorylated by ERK1, which was, itself, activated by cell adhesion and platelet-derived growth factor. Such phosphorylation of SPIN90 likely promotes the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex and Nck. It thus appears that the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex with Nck is crucial for stable cell adhesion and can be dynamically modulated by SPIN90 phosphorylation that is dependent on cell adhesion and ERX activation. SPIN90 directly binds syndapin I, syndapin isoform II-1 and II-s via its PRD region in vitro, in vivo and also associates with endocytosis core components such as clathrin and dynamin. In neuron and fibroblast, SPIN90 colocalizes with syndapins as puntate form, consistent with a role for SPIN90 in clathrin-mediated endocytosis pathway. Overexpression of SPIN90 N-term inhibits receptor-mediated endocytosis. Interestingly, SPIN90 PRD, binding interface of syndapin, significantly blocks internalization of transferrin, demonstrating SPIN90 involvement in endocytosis in vivo by interacting syndapin. Depletion of endogenous SPIN90 by introducing $\alpha-SPIN90$ also blocks receptor-mediated endocytosis. Actin polymerization could generate farce facilitating the pinch-out event in endocytosis, detach newly formed endocytic vesicle from the plasma membrane or push out them via the cytosol on actin tails. Here we found that SPIN90 localizes to high actin turn over cortical area, actin-membrane interface and membrane ruffle in PDGF treated cells. Overexpression of SPIN90 has an effect on cortical actin rearrangement as filopodia induction and it is mediated by the Arp2/3 complex at cell periphery. Consistent with a role in actin organization, CFP-SPIN90 present in actin comet tail generated by PIP5 $kinase\gamma$ overexpression. Therefore this study suggests that SPIN90 is functional linker between endocytosis and actin cytoskeleton.

• 한국식품저장유통학회지
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• 제18권3호
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• pp.310-318
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• 2011

양파의 매운 맛과 냄새를 감소시켜 섭취가 용이하도록 흑양파를 제조하고자 하였으며, 제조조건을 선발하기 위해서 숙성온도, 양파 크기, 그리고 양파 외부의 처리방법을 다르게 하여 흑양파를 제조하고, 각각의 흑양파 물 추출물에 대한 항산화 효과를 평가함으로서 흑양파를 새로운 식품 소재로 사용할 수 있는 흑양파 제조의 기초자료를 조사하였다. 온도에 따른 흑양파 제조효과에서 온도 증가에 따라 갈변효과가 증가하였다. 양파의 크기별은 100 g의 작은 양파와 뿌리와 줄기를 자른 양파의 갈변효과가 좋았다. 생양파보다 제조한 흑양파의 색도와 ${\Delta}E$ 값은 크게 증가하였으며 $90^{\circ}C$일 때 5.53 이었고, 총 페놀 함량은 온도의 증가에 따라 증가하는 경향을 보였는데 $90^{\circ}C$에서 9.19 mg/g이었다. $90^{\circ}C$에서 껍질 있는 작은 양파의 총 페놀함량은 11.62 mg/g으로 가장 높았다. 각 항산화 효과를 놓고 본 때 $90^{\circ}C$에서 껍질있는 작은 흑양파의 효과가 가장 좋았으며, DPPH 라디칼 소거능은 48%에 달하였고 아질산염 소거능도 타 처리구 보다 좋은 결과이었다. 흑양파 제조방법에 따른 기능성 성분을 분석한 결과 박피하지 않고 뿌리와 줄기를 자른 90-S-C 처리구가 갈변도, 총 페놀의 함량이 가장 높게 검출되었다. 이상의 결과를 종합하여 볼 때 $90^{\circ}C$에서 껍질로 싸여 있는 100 g 작은 양파의 갈변효과가 가장 우수하였다.

• 원예과학기술지
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• 제31권2호
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• pp.179-185
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• 2013

파프리카 수경재배용으로 사용되고 있는 암면을 대체하기 위해 개발 배지인 Phenolic foam(LC, Oasis Co.)의 물리특성에 맞는 양액공급방법을 구명하기 위해 양액공급 방법을 누적일사량 $100J{\cdot}cm^{-2}$에 주당 1회 공급량 100mL(100-100)를 대조구로 $90J{\cdot}cm^{-2}$에 주당 1회 공급량 90mL(90-90), $90J{\cdot}cm^{-2}$에 주당 1회 공급량 80mL(90-80) 등 3처리를 두고 Rockwool 배지와 함께 비교하였다. 양액공급 방법별 배액률은 100-100이 Rockwool에서 33.8%, LC에서 36.7%로 높았고 90-80이 Rockwool에서 30.4%, LC에서 33.7%로 낮았다. Rockwool 배지의 함수률과 EC는 63.6-68.9%, $4.4-5.dS{\cdot}m^{-1}$, LC 배지는 52.9-58.8%, $5.5-6.5dS{\cdot}m^{-1}$를 유지하였다. 100-100과 90-90 처리는 90-80 처리보다 생육이 촉진되어 과실이 크고 평균과중이 무거웠다. 착과수는 90-80 처리가 Rockwool 배지에서 주당 8.0개, LC 배지에서 8.3개로 가장 많았다. 상품과수는 90-90 처리가 가장 많았고 비상품 과수는 90-80 처리가 소과와 배꼽썩음과의 발생이 많아 주당 1.7-1.8개로 가장 많았다. 수량은 90-90 처리가 높았다.

• 한국환경보건학회지
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• 제20권3호
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• pp.61-77
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• 1994

Sprague Dawley rats were exposed to electric fields (6,000 V, 10 kV/m, 30 min/day, 6 days per week) and injected strontium 90 (681 kbq/rat, one time) through abdominal cavity (strontium 90 and electric field complexed exposure group). In parallel, series with the electric field exposure only, strontium 90 injection only and control groups were run. Every group was consisted of 110 rats (55 male and 55 female). This animal experiment was performed from May to December in 1993. This results were conducted to investigate the effect of electric field for 11 weeks. The results are summarized as follows: 1. Ornithine decarboxylase (ODC) activity in rat's bone marrow cells: The ODC values was significantly increased in Sr$^{90}$ injection group and Sr$^{90}$ and electric field complexed exposure group as compared with that of control group (p<0.05). The ODC value was significantly decreased in electric field and Sr$^{90}$ complexed exposure group in comparison with Sr$^{90}$ injection group (p<0.05). The ODC values of electric field only exposure group was not different to that of control group (p>0.05). 2. The amount of Sr$^{90}$ accumulation in the femur, kidney and spleen:The accumulation amount of Sr$^{90}$ in the femur of Sr$^{90}$ injection group represented higher value than that of electric field and Sr$^{90}$ complexed exposure group (p<0.05). In the kidney and spleen, the difference between electric field and Sr$^{90}$ complexed exposure group and Sr$^{90}$ injection group wasn't observed. 3. The counts of white cells in blood of Sr9?injection group was decreased as compared with the value from control group and electric field and Sr$^{90}$ complexed exposure group (p<0.05). The rat's body weight, red blood cell counts and the weight data of liver, kidney and spleen did not show differences among four groups.