• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.937-943
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• 2014

Polymorphisms in miRNA binding sites have been shown to affect miRNA binding to target genes, resulting in differential mRNA and protein expression and susceptibility to common diseases. Our purpose was to predict SNPs (single nucleotide polymorphisms) within miRNA binding sites of inflammatory genes in relation to gastric cancer. A complete list of SNPs in the 3'UTR regions of all inflammatory genes associated with gastric cancer was obtained from Pubmed. miRNA target prediction databases (MirSNP, Targetscan Human 6.2, PolymiRTS 3.0, miRNASNP 2.0, and Patrocles) were used to predict miRNA target sites. There were 99 SNPs with MAF>0.05 within the miRNA binding sites of 41 genes among 72 inflammation-related genes associated with gastric cancer. NF-${\kappa}B$ and JAK-STAT are the two most important signaling pathways. 47 SNPs of 25 genes with 95 miRNAs were predicted. CCL2 and IL1F5 were found to be the shared target genes of hsa-miRNA-624-3p. Bioinformatic methods could identify a set of SNPs within miRNA binding sites of inflammatory genes, and provide data and direction for subsequent functional verification research.

• Journal of Korean Neurosurgical Society
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• v.63 no.4
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• pp.477-486
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• 2020

Objectives : Due to recent developments and the wide application of percutaneous transforaminal discectomy (PTED) in China, we herein compare its clinical effects with microendoscopic discectomy (MED) for the treatment of lumbar disc herniation in terms of recurrence and revision rates. Methods : Six databases, namely, PubMed, EMBASE, Cochrane Library, Ovid, China National Knowledge Infrastructure and Wanfang, were searched by computer. The literature was screened according to inclusion and exclusion criteria, and the quality of the included literature was evaluated. After extracting the data from the papers, Review Manager 5.2 software (Cochrane Collaboration, Oxford, UK) was applied to analyze these data. Finally, sensitivity and publication bias analyses of the results were conducted. Results : A total of 12 studies consisting of 2400 patients were included in this meta-analysis. A comparison of PTED with MED revealed higher postoperative recurrence and postoperative revision rates for PTED (odds ratio [OR] recurrence, 1.60; 95% confidence interval [CI], 1.01 to 2.53; p=0.05 and OR revision, 1.77; 95% CI, 1.18 to 2.64, p=0.006). Conclusion : PTED has a number of advantages because it is a minimally invasive surgery, but its recurrence and revision rates are higher than MED. Therefore, MED should not be completely replaced by PTED.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.2
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• pp.707-711
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• 2013

Purpose: To investigate the clinical features and prognosis of papillary thyroid carcinoma (PTC) with a background of benign disease. Method: A total of 709 patients with papillary thyroid carcinoma undergoing surgical resection were analyzed retrospectively. In 147 patients who underwent surgery for benign thyroid disease, incidental PTC (IPC group) were identified by intraoperative or postoperative pathological examination of surgical specimens but were not detected by preoperative imaging studies. In the other group, according to the pathological examination with or without co-existing benign thyroid disease, 253 cases were clarified as concomitant PTC and 309 cases were clarified as dominant PTC. Results: Incidental PTC was more common in women, about 85.7%, the mean age was $47.6{\pm}11.3$ years old. Average tumor diameter was $4.4{\pm}2.2$ mm, multiple lesions accounted for 12.9% (19/147), and the cervical lymph node metastasis rate was 6.1% (9/147). After radical resection 8 cases recurred, the median time of recurrence was about 12 months (0.5 to 162), there was no tumor-related death. The tumor-free survival rates were 97.3%, 95.9%, 91.5%, and 79.3% in 1, 5, 10 and 14 year respectively. Conclusion: Incidental PTC with a background of benign lesions is common, and the generally good prognosis can be attributed to tumor early detection and early treatment. On the intraoperative finding of incidental PTC, lobectomy (unilateral) or total thyroidectomy (bilateral) should be the first choice, but with a postoperative pathologic finding of incidental PTC, further treatment, such as completion thyroidectomy or immediate lymph dissection is not necessary. Central lymph node dissection is also not needed unless lymphadenectasis is present.

• Journal of Power Electronics
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• v.16 no.2
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• pp.685-694
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• 2016

This paper presents a reliability assessment model for the power semiconductors used in wind turbine power converters. In this study, the thermal loadings at different timescales of wind speed are considered. First, in order to address the influence of long-term thermal cycling caused by variations in wind speed, the power converter operation state is partitioned into different phases in terms of average wind speed and wind turbulence. Therefore, the contributions can be considered separately. Then, in regards to the reliability assessment caused by short-term thermal cycling, the wind profile is converted to a wind speed distribution, and the contribution of different wind speeds to the final failure rate is accumulated. Finally, the reliability of an actual power converter semiconductor for a 2.5 MW wind turbine is assessed, and the failure rates induced by different timescale thermal behavior patterns are compared. The effects of various parameters such as cut-in, rated, cut-out wind speed on the failure rate of power devices are also analyzed based on the proposed model.

• Parasites, Hosts and Diseases
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• v.35 no.1
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• pp.19-24
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• 1997

The infection status of anisakid larvae was examined in 290 marine fish of 25 species and in 108 cephalopods of 3 species purchased in Bayuquan region, Yingko city nearby the coast of the Bohai Sea from may to August 1992. A total of 7,327 laivae were collected from 156 fish of 19 species and 8 squids of one species. The 3rd-stage larvae of Anisokis simplex were collected from 121 fish (63..4%) of 15 species (N : 191) and from 8 squids (14.8%) of one species (N : %4), and they were total, 5,992 (81.8%). Out of remaining 1,335 larvae, 154 (2.1%) were classified as Rvnnqscnris type B from 23 fish of 4 species, 1,013 (13.8%) as avnnosccris type C from 79 fish of 13 species, 164 (2.2%) as Hvsterothylacium China type V from 20 fish of 4 species, 3 (0.04%) as Raphinoscnris from 3 fish of 2 species and one was Pseunotewonovc necipiens larva. Key words: Bohai Sea, Anisokinae, larvae , Anisckis, HvsterothWLacium, Thwnnascaris, Raphinqscaris , Pseudoterranova.

• Biomolecules & Therapeutics
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• v.23 no.5
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• pp.400-406
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• 2015

MicroRNAs (miRNAs) are a family of non-coding RNA that are able to adjust the expression of many proteins, including ATP-binding cassette transporter and organic cation transporter. We sought to evaluate the effect of miR-511 on the regulation of OATP1B1 expression by free fatty acids. When using free fatty acids to stimulate Chang liver cells, we found that the expression of miR-511 increased significantly while the expression of OATP1B1 decreased. We also proved that SLCO1B1 is the target gene of miR-511 with a bioinformatics analysis and using the dual luciferase reporter assay. Furthermore, the expressions of SLCO1B1 and OATP1B1 decreased if transfecting Chang liver cells with miR-511, but did not increase when transfecting the inhibitors of miR-511 into steatosis cells. Our study indicates that miR-511 may play an important role in the regulation of OATP1B1 expression by free fatty acids.

• Journal of Microbiology and Biotechnology
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• v.30 no.11
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• pp.1688-1696
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• 2020

Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH₄⁺-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.

• Proceedings of the Ginseng society Conference
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• 1998.06a
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• pp.190-198
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• 1998

1 . Experimental study Preventive effect of Korea Red Ginseng (KPG) on hypertensive retinal arteriolosis in rabbits was studied. The results as follows: Blood pressure: Hypertensive group (B) was obviously raised up in comparing with that in normal group(A) and in hypertension + KRG group(C). Ocular fundus:Changes in B group including the retinal arteriospasm, crossing arterioveous, exudation and edema. But C group showed lightly. Light microscope: HE stained vascular damage in retina including thickness hyalimisation, execdates and edema Electron microscope: The endothelial cells were arranged irregularly, different shape and showed cytoplasm loose and vacuole. Immunohistochemistry: Ginseng can regulating endothelin-1, angiotension-ll, endothelium grow factor expre,j,iion and secreation in retinal blood clrultion. 2. ClinicAl Study 66 of hypertensive patients (42 men, 23 women,48-68 years old)and 20 normal person (7 men, 13 women,47-68 years old) were administrated(p.0.) by HRG (3g per day for 6 weeks). The results showed that marked effective rate and total effective rate were 53cyo and 60.6alo respectively and no severe side effects were found. The above results suggest that Ginseng have a difinite hypotensive effect and a role of preventing hyperfine sloe arteriosclerosis.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.1
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• pp.459-464
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• 2006

It has been well proved that shiphandling simulators are efficient and useful facilities for training and education of cadets and seafarers. Most of the maritime universities and many maritime training institutes all over the world have installed shiphandling simulators, which play important roles in maritime education and training. However, most of the Shiphandling simulators are standalone facilities with diversities on system architectures, layouts and functions. STCW78/95 requires simulators used for simulator-based training shall be suitable for the selected objectives and training tasks. To ensure the shiphandling simulator facilities meet the requirements of STCW convention and other expanded applications, collaborated research and coordination are needed in development and application of shiphandling simulators. Performance standard should be established for shiphandling simulator systems considering the advanced research needs as well as the needs in education, training, and assessment of competence. Standardizing and exchanging shiphandling mathematical models will improve critical performance of the system. Cooperated research on model course and training assessment approaches will enhance the training standard. In addition, the rapid spread of the internet technology has shown a promising future of application of shiphandling simulators through internet. Research has been carried out on internet based integration of multiple shiphandling simulators. A multi-agent based system, including necessary hardware, has been developed. Collaborated operation of the system can be of benefit in filling the gaps of the technical and operational level and methodology between maritime universities, enhancing mutual understanding of the navigation customs and culture background among cadets and seafarers from different countries, facilitating communication and maritime English training, and extending the functions of shiphandling simulators.

• Parasites, Hosts and Diseases
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• v.58 no.2
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• pp.181-184
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• 2020

Blastocystis, an enteric protist, has been reported to be an important cause of protozoal gastrointestinal manifestations in humans and animals worldwide. Animals harboring certain Blastocystis subtypes (STs) may serve as a potential source of human infection. However, information about the prevalence and genetic diversity of Blastocystis in alpacas is limited. In the present study, a total of 366 fecal samples from alpacas in Shanxi Province, northern China, were examined for Blastocystis by PCR amplification of the small subunit rRNA gene, followed by sequencing and phylogenetic analysis. The prevalence of Blastocystis in alpacas was 23.8%, and gender difference in the prevalence of Blastocystis was observed. The most predominant Blastocystis ST was ST10, followed by ST14 and ST5. The detection of ST5, a potentially zoonotic genotype, indicates that alpacas harboring ST5 could be a potential source of human infection with Blastocystis. These data provide new insight into the prevalence and genetic diversity of Blastocystis in alpacas.