Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid

  • Peng, Jin Fu (Department of Pharmacy, The Third Xiangya Hospital, Central South University) ;
  • Liu, Li (Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University) ;
  • Guo, Cheng Xian (Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University) ;
  • Liu, Shi Kun (Department of Pharmacy, The Third Xiangya Hospital, Central South University) ;
  • Chen, Xiao Ping (Institute of Clinical Pharmacology, Central South University) ;
  • Huang, Li Hua (Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University) ;
  • Xiang, Hong (Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University) ;
  • Huang, Zhi Jun (Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University) ;
  • Yuan, Hong (Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University) ;
  • Yang, Guo Ping (Center of Clinical Pharmacology, The Third Xiangya Hospital, Central South University)
  • Received : 2015.01.16
  • Accepted : 2015.07.23
  • Published : 2015.09.01
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Abstract

MicroRNAs (miRNAs) are a family of non-coding RNA that are able to adjust the expression of many proteins, including ATP-binding cassette transporter and organic cation transporter. We sought to evaluate the effect of miR-511 on the regulation of OATP1B1 expression by free fatty acids. When using free fatty acids to stimulate Chang liver cells, we found that the expression of miR-511 increased significantly while the expression of OATP1B1 decreased. We also proved that SLCO1B1 is the target gene of miR-511 with a bioinformatics analysis and using the dual luciferase reporter assay. Furthermore, the expressions of SLCO1B1 and OATP1B1 decreased if transfecting Chang liver cells with miR-511, but did not increase when transfecting the inhibitors of miR-511 into steatosis cells. Our study indicates that miR-511 may play an important role in the regulation of OATP1B1 expression by free fatty acids.

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References

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