• BMB Reports
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• v.40 no.6
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• pp.944-951
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• 2007

3-Hydrogenkwadaphnin (3-HK) is a daphnane-type diterpene ester isolated from Dendrostellera lessertii (Thymelaeaceae) with high differentiation and apoptotic potency in leukemic cells without any measurable adverse effects on normal cells (Moosavi et al., 2005b). In this study, we report that 3-HK (12 nM) has the ability to cease proliferation, induce differentiation and apoptosis in chronic myelogenous leukemia (CML) K562 cell line. The treated cells lost erythroid properties and differentiated along the megakaryocytic lineage based on the morphological features apparent after Wright-Giemsa staining, DNA content analysis and the expression of cell surface marker glycoprotein IIb as analyzed by flow cytometry. Moreover, using Hoechst 33258 and Annexin V double staining indicated the occurrence of apoptosis among the treated cells. On the other hand, restoration of the depleted GTP pool size by exogenous addition of guanosine ($50{\mu}M$) reduced the effect of the drug regarding the extent of differentiation while no further enhancement of 3-HK effect was obtained by addition of exogenous hypoxanthine ($100{\mu}M$). These interesting results necessitate further investigation regarding the mechanism of action of this unique anti-leukemic agent.

• The Korean Journal of Cytopathology
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• v.6 no.1
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• pp.62-66
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• 1995

Peripheral neuroepithelioma (PNE) of soft tissue is a malignant neuroectodermal tumor arising from peripheral (nonautonomic) nerve. It nay occur in both children and adults, and are highly aggressive neoplasms that rapidly give rise to metastatic disease and death. We exprienced a case of peripheral neuroepithelioma of soft tissue in the upper arm in a 18-year-old female. Cytologic features revealed small round cells with scanty cytoplasm occurring both singly and in clusters. The clusters frequently tended to form Homer-Wright rosettes. The cells had a round to oval nucleus with fine chromatin and inconspicuous nucleoli in a hemorrhagic background.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.7
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• pp.3073-3076
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• 2012

Terpinen-4-ol is a terpene found in the rhizome of Plai (Zingiber montanum (Koenig) Link ex Dietr.). In this study apoptogenic activity and mechanisms of cell death induced by terpinen-4-ol were investigated in the human leukemic MOLT-4 cell line. Terpinen-4-ol exhibited cytotoxicity in MOLT-4 cells, with characteristic morphological features of apoptosis by Wright's staining. The mode of cell death was confirmed to be apoptosis by flow cytometric analysis after staining with annexin V-FITC and propidium iodide. A sub-G1 peak in DNA histograms of cell cycle assays was observed. Terpinen-4-ol induced-MOLT-4 cell apoptosis mediated through an intrinsic pathway involving the loss of mitochondrial transmembrane potential (MTP) and release of cytochrome c into the cytosol. In addition, terpinen-4-ol also induced apoptosis via an extrinsic pathway by caspase-8 activation resulting in the cleavage of cytosolic Bid. Truncated-Bid (tBid) translocated to mitochondria and activated the mitochondrial pathway in conjunction with down-regulation of Bcl-2 protein expression. Caspase-3 activity also increased. In conclusion, terpinen-4-ol can induce human leukemic MOLT-4 cell apoptosis via both intrinsic and extrinsic pathways.

• BMB Reports
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• v.50 no.10
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• pp.522-527
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• 2017

A large number of transcriptional activation domains (TADs) are intrinsically unstructured, meaning they are devoid of a three-dimensional structure. The fact that these TADs are transcriptionally active without forming a 3-D structure raises the question of what features in these domains enable them to function. One of two TADs in human glucocorticoid receptor (hGR) is located at its N-terminus and is responsible for ~70% of the transcriptional activity of hGR. This 58-residue intrinsically-disordered TAD, named tau1c in an earlier study, was shown to form three helices under trifluoroethanol, which might be important for its activity. We carried out heteronuclear multi-dimensional NMR experiments on hGR tau1c in a more physiological aqueous buffer solution and found that it forms three helices that are ~30% pre-populated. Since pre-populated helices in several TADs were shown to be key elements for transcriptional activity, the three pre-formed helices in hGR tau1c delineated in this study should be critical determinants of the transcriptional activity of hGR. The presence of pre-structured helices in hGR tau1c strongly suggests that the existence of pre-structured motifs in target-unbound TADs is a very broad phenomenon.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.2145-2148
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• 2012

Objective: This study concerns expression of PBK/TOPK during differentiation of HL-60 leukemic cells induced by tetradecanoyl phorbol acetate (TPA). Methods: Wright-Giemsa staining was performed to observe morphological changes in the HL-60 cells, and flow cytometry was used to assess the cell cycle and CD11b, CD14, CD13, and CD33 expression. PBK/TOPK levels were determined by Western blot analysis. Results: After treating HL60 cells with $5.1{\times}10^{-9}$ mmol/L of TPA for three days, the number of nitroblue-tetrazolium-positive cells and CD11b, CD13, and CD14 expression increased, whereas the PBK/TOPK levels decreased. Conclusions: TPA can inhibit proliferation and induce differentiation of HL60 cells of the granulocytic or monocytic lineage. PBK/TOPK expression was downregulated during this process, whereas the Pho-PBK/TOPK expression was increased.

• Journal of Korean Academy of Nursing
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• v.15 no.2
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• pp.25-33
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• 1985

This study intended to investigate the volume of respiration according to the postoperative time and positions among the upper abdominal surgery patients. Tidal volume and vital capacity were measured in three positions-supine, left lateral and sitting position-at preoperatively, 12 hours postoperatively and 36 hours postoperatively. Thirteen male and seven female patients who were admitted for elective abdominal surgery under general anesthesia were the subjects of the study. Those patients with cardiopulmonary problems, obesity and smoking habit were excluded from the study. The study was conducted from March 15 to June 30, 1985 in Seoul National University Hospital. Tidal volume and vital capacity were measured by Wright spirometer in various positions at preoperatively, 12 hours postoperatively and 36 hours postoperatively. The results were as following: 1) Vital capacity was significantly decreased at 12 hours preperatively and 36 hours postoperatively than preoperatively. Vital capacity was not significantly different in Various positions, but sitting position revealed better than left lateral and supine position. Tidal volume was not significantly different in each position. 2) Male patients showed significantly higher than female patients in tidal volume and vital capacity. Vital Capacity was not significantly different by sex in each position, but vital capacity was higher in sitting position than in lateral and supire position. 3) There was not significantly different in tidal volume and vital capacity according to the type of incision and positions, vital capacity was higher in sitting positionthan in left lateral and supine position.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.1876-1881
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• 2003

When a projectile travels at high speed underwater, supercavitating flow arises, in which a huge cavity is generated behind the projectile so that only the nose, i.e., the cavitator, of the projectile is wetted, while the rest of it should be surrounded by the cavity. In that case, the projectile can achieve very high speed due to the reduced drag. Furthermore if the nose of the body is shaped properly, the attendant pressure drag can be maintained at a very low value, so that the overall drag is also reduced dramatically. In this study, shape optimization technique is employed to determine the optimum cavitator shape for minimum drag, given certain operating conditions. Shape optimization technique is also used to solve the potential flow problem for any given cavitator, which is a free boundary value problem having the cavity shape as unknown a priori. Analytical sensitivities are derived for various shape parameters in order to implement a gradient-based optimization algorithm. Simultaneous optimization technique is proposed for efficient cavitator shape optimization, in which the cavity and cavitator shape are determined in a single optimization routine.

• Journal of Biosystems Engineering
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• v.11 no.2
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• pp.4-13
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• 1986

뿌리작물(作物)을 수확(收穫)하기 위(爲)하여 진동(振動)을 이용(利用)한 4절(節)링크로 구성(構成)되는 시작기(試作機)를 설계(設計), 제작(製作) 및 실험을 수행(遂行)하였다. 수확기(收穫機)의 작동중(作動中) 발생(發生)하는 불균형력(不均衡力)을 상쇄(相殺)시키기 위(爲)한 힘의 평형장치(平衡裝置)를 설계(設計)하기 위(爲)하여 각(各) 요소(要素)의 가속도(加速度) 및 동력학적(動力學的)인 힘을 분석(分析)하였고, 진동기구(振動機構)에 의(依)하여 발생(發生)되는 이론적(理論的)인 토크와 측정(測定)된 토크를 비교분석(比較分析)하였다. 설계(設計)된 힘의 평형장치(平衡裝置)는 불균형력(不均衡力)의 대부분(大部分)을 상쇄(相殺)시켜주고, 이론적(理論的)인 토크는 실측(實測)토크의 변화경향(變化傾向)과 잘 일치(一致)하였다.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.10a
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• pp.89-110
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• 2003

The limitations of conventional soil and groundwater contamination remediation technologies have motivated a search for innovative technologies; particularly in situ technologies that do not require extraction of contaminants from the subsurface. All engineered in situ remediation systems require that the contaminant be mixed with a remedial compound. Horizontal flow treatment wells (HFTWs), an innovative technology that consists of a pair of dual-screened treatment wells, were used at a trichloroethylene (TCE) contaminated site to efficiently achieve this mixing of contaminant and remedial compound in order to effect in situ bioremediation (McCarty et al., 1998). In this paper, the potential of HFTWs to treat chlorinated aliphatic hydrocarbons (CAHs) as well as other soil and groundwater contaminants of concern, such as nitroaromatic compounds (NACs), perchlorate, and methyl-tert-butyl ether (MTBE), is examined. Through a combination of laboratory studies, model analyses, and field evaluations, the effectiveness of this innovative technology to manage these contaminants is investigated.

• Korean Journal of Veterinary Research
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• v.46 no.4
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• pp.387-393
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• 2006

Progressing to the iron deficiency anemia was experimentally induced in 4 clinically healthy dogs by repeated phlebotomy to characterize hematologic features, serum iron values, and RBC indices. Abnormal RBC morphologies were also evaluated semiquantitatively on Wright's-stained blood films. Hematologic abnormalities in early stage of anemia included decreased both hematocrit and hemoglobin, and reticulocytosis, with no changes in mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were represented. In intermediate stage, decreased serum iron concentration with microcytosis and hypochromia were prominent. In late stage, red cell distribution width and Mentzer's index were out of reference ranges in the majority of dogs. In this study microcytic anemia was appeared at the hemoglobin range of 5.1-7.2 g/dl. On most sampling days, platelet counts and white blood cells were within the reference ranges, with some minor variations. Iron deficiency was not necessarily associated with microcytic anemia. Judging from the sequential changes of both MCV and MCHC, 3 patterns of anemia were sequentially observed: initially normocytic normochromic, intermediate normocytic hypochromic or normocytic normochormic, and finally microcytic hypochromic. The most frequent morphologic abnormalities were target cells. Occasional elliptocyte, acanthocyte, stomatocyte, kinzocyte, dacrocyte and schistocyte were also noted on the blood films.