Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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PBK/TOPK Expression During TPA-Induced HL-60 Leukemic Cell Differentiation

  • Liu, Yu-Hong (Department of Hematology, The Affiliated Hospital of Yan'an University) ;
  • Gao, Xue-Mei (Department of Hematology, The Affiliated Hospital of Yan'an University) ;
  • Ge, Fan-Mei (Department of Hematology, The Affiliated Hospital of Yan'an University) ;
  • Wang, Zhe (Department of Pathology, School of Basic Medicine, Fourth Military Medical University) ;
  • Wang, Wen-Qing (Department of Pathology, School of Basic Medicine, Fourth Military Medical University) ;
  • Li, Xiao-Yong (Department of General Surgery, The Affiliated Hospital of Yan'an University)
  • Published : 2012.05.30
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Abstract

Objective: This study concerns expression of PBK/TOPK during differentiation of HL-60 leukemic cells induced by tetradecanoyl phorbol acetate (TPA). Methods: Wright-Giemsa staining was performed to observe morphological changes in the HL-60 cells, and flow cytometry was used to assess the cell cycle and CD11b, CD14, CD13, and CD33 expression. PBK/TOPK levels were determined by Western blot analysis. Results: After treating HL60 cells with $5.1{\times}10^{-9}$ mmol/L of TPA for three days, the number of nitroblue-tetrazolium-positive cells and CD11b, CD13, and CD14 expression increased, whereas the PBK/TOPK levels decreased. Conclusions: TPA can inhibit proliferation and induce differentiation of HL60 cells of the granulocytic or monocytic lineage. PBK/TOPK expression was downregulated during this process, whereas the Pho-PBK/TOPK expression was increased.

Keywords

References

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