• Title/Summary/Keyword: Women infertility

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Molecular Genetic Analysis of Microdeletions in Y Chromosome from Korean Male Infertility Patients (한국인 남성 불임환자에서 Y염색체내 미세결실의 분자유전학적 분석)

  • Yoon, Hyun-Soo;Lee, Jeong-Hen;Seo, Ju-Tae;Kim, Hae-Jung;Lee, Dong-Ryul;Jeon, Jong-Sik;Cho, Jung-Hyun;Kim, Moon-Kyoo;Lee, Moo-Sang;Roh, Sung-Il
    • Clinical and Experimental Reproductive Medicine
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    • v.23 no.3
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    • pp.367-377
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    • 1996

  • Genes on the long arm of Y chromosome, particularly interval 6, are believed to playa critical role in human spermatogenesis. The objective of this study was to validate a sequenced-tagged site(STS)-mapping strategy for the detection of Yq microdeletion and to use this method to determine the proportion of men with Yq microdeletions in idiopathic, obstructive, nonobstructive azoospermia, severe OATS and in normal males. We analyzed three STS markers mapped to interval 6 within long arm of the Y chromosome from 106 nonobstructive, 30 obstructive azoospermia, 15 severe OATS patients, and normal 42 males in Korean men. By PCR, we tested leukocyte DNA, for the presences of STS markers(DAZ, sY129 and sY134) and SRY gene as internal control. And PCR results were confirmed by Southern hybridization, and were investigated by SSCP analysis for DAZ gene mutation. None of 42 normal males and 30 obstructive azoospermia had microdeletions, Of the 15 severe OATS typed with DAZ, sY129 and sY134, 3(20.0%) patients failed to amplify 1 or more STS markers, and of the 106 nonobstructive azoospermia typed with DAZ, sY129 and sY134, 12(11.3%) patients failed to amplify 1 or more STS markers. From these results, high prevalence(12.4%) of Yq deletion(DAZ, sY129, sY134) in men with nonobstructive idopathic azoospermia and severe OATS were observed in Korean infertility patients. To avoid the infertile offspring by assisted reproductive technique using ICSI or ROSI, genetic diagnosis will be needed in IVF-ET program.

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Analysis of Factors Affecting Survival and Pregnancy Rate in Frozen-thawed Embryo Transfers (동결수정란 이식주기에서 수정란 융해 후 생존율과 임신율에 영향을 미치는 요인)

  • Kim, Jeong-Wook;Byun, Hye-Kyung;Youm, Hye-Won;Jun, Jin-Hyun;Park, Yong-Seog;Song, In-Ok;Song, Ji-Hong;Choi, Bum-Chae;Koong, Mi-Kyoung;Jun, Jong-Young;Kang, Inn-Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.1
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    • pp.59-65
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    • 2000

  • Objective: The purpose of this study was to determine the important factors affecting survival and pregnancy rate in frozen-thawed embryo transfer cycles. Methods: we performed retrospective analysis in 738 cycles of frozen-thawed embryo transfers, in relation to the insemination methods, the freezing stage of embryo, patient's age, infertility factors and the origin of injected sperm in ICSI cycles. After conventional IVF or ICSI, the supernumerary PN stage zygotes or multicellular embryos were cryopreserved by slow freezing protocol with 1,2-propanediol (PROH) as a cryoprotectant. Results: The survival rates of thawed embryos were 69.3% (1585/2287) in conventional IVF group and 71.7% (1645/2295) in ICSI group. After frozen-thawed embryo transfers, 27.0% (92/341) and 32.0% (109/341) of pregnancy rates were achieved in conventional IVF and ICSI group, respectively. There were no significant difference in the survival and pregnancy rates according to the insemination methods, the freezing stage and patient's age. However, the pregnancy rate (36.2%) of male factor infertility was significantly higher than the tubal (27.2%) and other female factor infertility (22.9%). In ICSI group, the origin of injected sperm did not affect the outcome of frozen-thawed embryo transfer cycles. Conclusion: The present study demonstrates that acceptable clinical outcomes can be achieved after the transfer of frozen-thawed embryos regardless of the stage of embryos for freezing, the patient's age and the origin of injected sperm.

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