• Journal of Preventive Medicine and Public Health
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• 제1권1호
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• pp.43-49
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• 1968

Epidemiological and statistical observations were made of fire hazards that occurred during the past 18 years, 1948 to 1965. Injury and mortality rates for all ages were computed chronologically. For the years of 1955, 1961 and 1965, all fire accidents were epidemiologically analysed to draw characteristic patterns in relation to the seasonal and 24 hour distribution, causes and sites of accidents etc.. Fire hazards observed herein are the categorys E 916 of the International Classification of Causes of Death, 1955, and includes all accidents caused by fire and explosion of combustible materials. The following conclusion was made: 1. The average number of annual deaths due to fire was 183 and the number of the in jured due to the same cause was 335. The mortality rate per 100,000 population was 0.8 and the ratio of injuries per death was 1.83. 2. The casually rate including both the dead and injured was 5.0 per 100,000 in Seoul, the highest among the provinces and followed by 3.4 in Cheju -Do, 2.1 in Kangwon-Do, 1.7 in Kyunggi-Do accordingly. The other provinces had a range of 0.6 to 1.2. 3. The monthly distribution of fro accidents showed that the winter months, December through February, had more frequent accidents, while the summer season, June through August had less. The 24 hour distribution of accidents showed more cases from 12:00 to 18:00 and less from 4:00 to 10:00 hours. 4. The per cent distribution of causes of accidents showed; 90.0% for careless, 10.0% for arson. The cause of carelessness was further breakdown into; 15.0% for kitchen fire places, 13.8% for fire playing, 9,4% for electrical heating and wires, 8.3% for fuels, 6.3% for matches, 5.2% for ash dumps and the remaining for others. 5. The accidents as classified by place revealed that 56.8% of the total occurred at the common dwelling houses, 11.3 at various industrial workshops, 9.3% at the street shops and the remaining at the miscellaneous places.

• 한국어병학회지
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• 제29권1호
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• pp.35-43
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• 2016

전복의 면역, 생리 및 생태에 관한 기초 연구는 매우 부족하며 전복 질병에 관한 연구 또한 어류에 비하여 극히 취약한 상태이다. 외국의 경우 전복 치패에서 vibrios로 인한 피해 사례가 많이 보고되어 있으나 우리나라에서는 세균성 질병으로 인한 전복 폐사에 관한 보고가 드물다. 그러나 폐사체로부터 vibrios가 높은 빈도로 분리되고 있어 이들 Vibrio속 세균과 전복 폐사와의 관련성에 대한 검토가 필요하다. 현재 전복 양식 현장에서는 질병으로 인한 피해를 최소화하기 위해 항균제를 사용하고 있지만 치료 효과는 미미한 수준이다. 이에 대한 대책으로 probiotics에 대한 요구가 증가하고 있어 본 연구에서는 인체에 위해하지 않은 균을 분리하여 전복 probiotics로서의 개발 가능성을 알아보았다. 잘 숙성된 김치액에서 분리된 균들 중 다양한 vibrios에 대하여 항균력을 나타낸 KC16-2을 candidate probiotic 균으로 선발하였다. KC16-2는 생화학적 특성과 16S rRNA gene sequence에 근거하여 Bacillus amyloliquefaciens로 동정되었다 (B. amyloliquefaciens KC16-2). B. amyloliquefaciens KC16-2는 in vitro 실험에서 병원성 비브리오에 대하여 광범위한 생장 억제 효과를 보였으며, 온도 $15{\sim}25^{\circ}C$의 해수에서 4일까지 일정수를 유지하였으므로 겨울 이외의 기간에는 사용이 가능하다고 판단되었다. 또한 담즙에 의해 생장이 억제되었으나 시간 경과에 따라 처음 투여 농도보다 증가하는 양상이 관찰되어 담즙이 있는 곳에서도 생존 가능하다고 판단되었다. 시판 사료에 KC16-2를 혼합하여 12주간 전복에 투여하였을 때 대조구에 비해 다소 좋은 성장을 보였으나 통계학적으로 유의한 차이는 없었다. 한편 전복에 병원성을 가진 Vibrio tubiashii의 인위감염실험에서 probiotic사료를 공급한 수조에서 폐사율을 약 50% 감소시키는 결과를 나타냄으로써 기회성 vibrios로 인한 전복의 폐사를 줄이기 위하여 KC16-2의 사용이 긍정적으로 검토될 수 있음을 시사하였다.

• 한국지리정보학회지
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• 제17권1호
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• pp.61-69
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• 2014

본 연구에서는 서울에서 직경 $10{\mu}m$이하의 미세먼지에 대한 공간분석을 수행하였다. PM10은 폐암과 질병에 의한 사망율을 증가시키는 주요 원인이기 때문에 PM10의 공간적 분포는 서울의 대기오염에서 중요한 관심 사항이다. 본 연구에서 PM10의 공간적 분포 분석은 2010년과 2011년 서울시 미세먼지의 월 평균자료에 의해 모니터링 되었다. PM10의 공간보간은 PM10의 공간분포 특성을 잘 반영하는 IDW 방법을 적용하였고, PM10의 월 평균 공간분포는 서울시의 서부지역(영등포)이 초봄과 겨울철에 서울시의 북부지역 보다 높은 농도의 공간 분포를 나타내었다. 또한 2010년과 2011년 PM10 농도 분포의 비교에서 2011년 강남구와 송파구의 PM10 농도는 2010년 연평균 공간분포 보다 증가하였다. 서울시 PM10의 공간 분포가 영등포구, 강남구, 청량리 등 특정지역에서 높은 경향을 나타내고 있어서 이에 대한 관리방안의 수립이 요구된다.

• 대한수의학회지
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• 제40권3호
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• pp.563-573
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• 2000

Periodic outbreaks of Newcastle disease (ND) caused by velogenic viscerotropic ND virus (vvNDV) has become a major concern in Korea nowadays. Throughout last epidemic, the winter season in 2000, most chicken flocks infected early, under 2-4 weeks of age, showed high mortality up to 50-100%. Serum samples collected from 201 breeder, 284 layer and 112 broiler chicken flocks were examined to evaluate the efficacy of various vaccination methods and programs routinely used for mass vaccination in the field poultry farms. Despite repeated live vaccination, most poultry flocks vaccinated by drinking water route using nipple water supply system failed to produce solid active immune response to NDV during the growing time. In the present study, we applied the spray vaccination technique using Ulvavac or Desvac sprayer to the experimental poultry flocks and examined the efficacy of live vaccination effects induced by it under field condition. Measurable antibody to NDV as well as early protection against vvNDV challenge were found in poultry flocks vaccinated by spray route. Further, we did not found significant post vaccination reactions caused by spray vaccination if properly administered. These data indicate that the spray vaccination will be safe and reliable mass vaccination method for the prevention of ND.

• 한국수산과학회지
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• 제28권5호
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• pp.667-676
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• 1995

군산 연안 조간대에 있어서 1993년 6월에서 1994년 5원까지 조사한 동죽(M. veneriformis)의 침강과 가입 및 성장, 생산량에 대하여 조사하였다. 동죽의 부유유생의 높은 밀도는 1993년 6월 19일에서 7월 3일에 관찰되었으며, 침강된 치패의 크기는 250um에서 350um였고, 평균밀도는 약 $601\;inds\cdot\;m^{-3}$이었다. 유생의 침강에 의한 가입은 주로 1993년 6월 중순에서 7월 중순에 이루어 졌으며, 유생의 침강시 저질에 따른 선택성은 없었다 그러나 침강한 치패는 환경(저질, 노출시간, 성패의 밀도)에 의하여 사망율이 결정되었다. 평균 각장에 의하여 추정한 동죽의 성장은 가입 초기에 있어서 과소평가 되었으며, 성장기는 11월 중순까지이고, 겨울철에는 정지하였다. 0세군의 연간생산은 2,900 g\cdot\;m^{-2}\;\cdot\;yr^{-1}$으로 나타났으며, 회전율은 약 4.94 (Crisp, 1984)와 4.91 (Ricker, 1978)로 추정 되었다.

• 한국환경과학회지
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• 제18권10호
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• pp.1171-1179
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• 2009

The solitary ascidian, Halocynthia aurantium, which is commonly called the sea peach because of its coloration and general shape, is a valuable organism of benthic marine population in the northern region of the East Sea, Korea. It is seldom found at a depth of less than 10 meters and the sea peach is frequently observed in large populations between 20 and 100 meters. It appears to prefer attachment to vertical rocks faces and artificial cement blocks exposed to the currents. Mass mortality and reduction of resources in sea peach, H. aurantium, were occurred in the benthic area of the northern region of the East Sea because of the rapid fluctuation of environmental factors such as temperature and salinity due to mass rainfall in summer and going up north of a strong warm current in winter. Therefore, we examined the effects of temperature and salinity on embryonic development of fertilized eggs, tadpole larva to metamorphosis, and attachment to siphon development. Laboratory-raised larvae were studied using a two-factorial experimental design with four levels of temperature(8, 12, 16 and $20^{\circ}C$) and four levels of salinity(20, 25, 30 and 34 psu). The ascidian larvae of H. aurantium survived environmental conditions between temperature of $8{\sim}20^{\circ}C$ and salinity of 25~34 psu and exhibited positive growth at $8{\sim}16^{\circ}C$ and 30~34 psu. Fertilized eggs have not developed at lower salinity of 20 psu irrespective of temperature range tested and have showed an abnormal development at the salinity of 25 psu between higher temperatures of 20 and $24^{\circ}C$. This result suggests that temperature increase and salinity reduction depending on environmental fluctuation may have significant impacts on population variation of H. aurantium in the northern region of the East Sea.

• 한국동물위생학회지
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• 제26권2호
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• pp.135-144
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• 2003

Fowl typhoid is a septicemic disease of turkeys and chickens. The antigen is Salmonella(S) gallinarum, which cross-reacts with S pullorum because of common antigen. The organism is maintained in the ovary of carrier birds and transmitted vertically in the york. It is also transmitted horizontally through feces and broken eggs. The symptoms are weakness, drowsiness, wing drooping, hyperexcitability, paresis and diarrhea. In the many cases, the birds are found dead in the morning before any symptoms have been noticed. this experiment was carried out to investigate the characteristics of S gallanarum and scrutinized about the control systems. 1. Fowl typhoid was came about in the winter than the other seasons. The average of mortality was 46.3%. And it was came about not only adult poultry but also chicks. 2. S gallinarum was isolated from liver and spleen in infected chickens. Total isolated strains were 60. 3. The organisms were all extinct at 60$^{\circ}C$, 30mins. 4. A survival rate was higher in underground water than a secondary distilled water. It was detected in underground water until 30 days. 5. When being exposed to the solar light, it became extinct almost within 24 hours. 6. It was survived in a refrigerator until 12 months. 7. It was extinct among 5 mins in 0.1% phenol and 2% formalin. 8. When the cultured organism fluid was inoculated in SD-rat abdominal cavity, rat died within 24 hours. 9. Pullorum-typhoid related program must disciplines in order to destroy the fowl typhoid, and breeding system must be converted to cage system.

• Child Health Nursing Research
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• 제5권2호
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• pp.136-150
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• 1999

The subjects, under 18 old, 2,694 children who visited ER during 1998, were surveyed by the Triage and other statitical data. The results were as follows 1. The male to female ratio was 1.7 : 1, and the toddler(1-4 old) was the majority. 2. Triage ; critical 1.3%, acute 14.6%, urgent 29.5%, nonemergent 54.6%. 3. The reasons of visiting ER ; 1) The children had diseases(46.5%), injury (36.9%), TA(5.6%) and toxication(1.0%). 2) In diseases, male to female ratio was 1.5 : 1 and in injury, male to female was 2 : 1. 3) Among the children having in TA, 12-18 old groups was the majority(34.9%). 4. The time of visiting : the 20:00 - 22:00 was the majority(16.9%). 5. By monthly and seasonal distribution ; Jan. (9.7%), Mar. and May(9% respectively, Dec. and July(6.7%) respectively. The children who visted ER in spring and autumn showed higher portion than those of summer and winter. 6. Results ; admission(27.4%), discharge(68.4%), operations(2.8%), and DOA and DAA(0.4%), The mortality of the infancy and toddler groups was 83.3%. The infancy group showed the highest rate of admission. 7. The time of staying in ER ; 1-2 hrs was the major group(23.3%) and the average was 4.6 hours. 8. By clinical departments ; Ped. was 34.4%, PS was 20.8%, Dental Surgery was 10.3% and Dermatology was 0.9%. 9. The types of visting ; the group who visited by themselves was 80%, transfer from the primary and secondary clinic was 17% and OPD was 3.0%. 10. The traffic means . by the own cars and taxi were 87.6%, by hospital ambulance was 6.1% and by 119 ambulance was 4.3%.

• Journal of Veterinary Science
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• 제22권3호
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• pp.40.1-40.12
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• 2021

Background: The microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen. Objectives: The present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees. Methods: A procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration. Results: UR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 10⁴ spores/bee, and the stable detection level was ≥ 2.40 × 10⁵ spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 10⁴ copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min. Conclusions: UR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection.

• 해양환경안전학회지
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• 제23권5호
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• pp.513-523
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• 2017

본 연구는 섬진강 하구역에서 2017년 2월 대조기 만조와 간조, 3월 소조기 간조 동안 염분 구배에 따른 식물플랑크톤 생리활성도를 평가하였다. 또한 2월 대조기 조사에서 나타난 특이적인 pH 변화특성을 파악하기 위해 추가적인 재현실험을 병행하였다. 현장조사 결과에 의하면, 만조와 간조에 수층 혼합에 의한 수직적인 염분의 차이는 미미하였지만, 담수역으로 향할수록 염분이 낮아져 수평적인 염분구배가 뚜렷하였다. 염분과 영양염(질산염+아질산염: R=0.997, p<0.001; 규산염: R=0.979, p<0.001)과의 강한 음의 상관관계를 보였고, 이는 담수 기원의 질소와 규소의 높은 농도의 영양염류가 해수와 혼합되는 과정에서 희석되고 있다는 것을 의미한다. 반면, 인산염농도는 염분이 15 psu를 기점으로 고염분의 유역에서는 상대적으로 높은 농도가 관찰되었고, 이는 수심이 얕은 광양만의 퇴적물이 조석에 의한 수층혼합으로 재부유되어 인산염 재용출로 인해 수층에 일정량 기여하였다는 것을 의미한다. 섬진강 하구역에서 동계 2월과 3월 우점한 식물플랑크톤은 Eucampia zodiacus로 나타났고, 그들은 전체 군집의 70% 이상을 차지하였다. 특히, 3월 생물 활성도를 평가한 결과, 염분과 chlorophyll a(R=0.82)뿐만 아니라 active chlorophyll a(R=0.80)과 강한 양의 상관관계를 보였다(p<0.001). 특히 담수의 영향을 강하게 받는 상류 정점에서는 해수종 규조류가 염분충격으로 생리활성을 잃어 최대 75 % 사멸율을 기록하였다. 2월 대조기 조사에서는 상대적으로 형광값이 높은 정점에서 pH가 높게 나타났고, 이는 높은 밀도로 우점한 규조류의 광합성 작용으로 $CO_2$가 다량 흡수되어 pH 상승효과를 가져온 것으로 판단되었다. 또한 2월 현장식물플랑크톤 군집을 농축하여 저염분 환경에서 생물농도구배에 따른 pH 변화를 파악한 결과, 염분충격에 의해 사멸한 식물플랑크톤의 영향으로 광합성 활성도의 감소를 가져왔고, 우점 규조류의 사멸로 인해 박테리아가 현저하게 증식하였다. 그 결과 박테리아가 규조류를 생분해하는 과정에서 $CO_2$가 발생하여 pH 의 감소를 유발하였다. 결과적으로 섬진강하구역에서는 염분 구배에 따른 생물의 광합성 활성도의 차이와 생물사멸이 뚜렷하게 관찰되었고, 이는 pH의 증감을 유발하는 중요한 인자로 파악되었다.