• Mycobiology
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• v.49 no.1
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• pp.61-68
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• 2021

Agaricus bisporus, commonly known as the button mushroom, is widely cultivated throughout the world. To breed new strains with more desirable traits and improved adaptability, diverse germplasm, including wild accessions, is a valuable genetic resource. To better understand the genetic diversity available in A. bisporus and identify previously unknown diversity within accessions, a phylogenetic analysis of 360 Agaricus spp. accessions using single-nucleotide polymorphism genotyping was performed. Genetic relationships were compared using principal coordinate analysis (PCoA) among accessions with known origins and accessions with limited collection data. The accessions clustered into four groups based on the PCoA with regard to genetic relationships. A subset of 67 strains, which comprised a core collection where repetitive and uninformative accessions were not included, clustered into 7 groups following analysis. Two of the 170 accessions with limited collection data were identified as wild germplasm. The core collection allowed for the accurate analysis of A. bisporus genetic relationships, and accessions with an unknown pedigree were effectively grouped, allowing for origin identification, by PCoA analysis in this study.

• Journal of Mushroom
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• v.20 no.4
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• pp.249-253
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• 2022

Fungi that infect mushrooms and cause diseases are called mycoparasitic fungi. Understanding the diversity of mycoparasitic fungi in wild mushrooms is important for the mushroom industry, as they can parasitize cultivated mushrooms. However, few studies have been conducted on the diversity of mycoparasitic fungi in wild mushrooms in South Korea. Upon surveying mushroom flora, we found a mycoparasitic fungus and analyzed its strain using molecular phylogeny and morphological examination. The strain was identified as Sepedonium laevigatum, which is an unrecorded species in South Korea. In this study, we described the cultural and microscopic characteristics of this strain of fungus.

• Journal of Mushroom
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• v.18 no.1
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• pp.84-90
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• 2020

Wild-type isolates of H. erinaceus were collected from different geographical areas of Korea. Nineteen isolates were cultured on mushroom substrate for producing fruiting bodies. Of these, 14 isolates formed pinheads and fruiting bodies on the substrate. The morphological and cultivation characteristics of fruiting bodies were categorized by pinheading, fruit body formation, spine types, fresh weights, and colors. Microstructures, including spines, spores, and basidia on the fruiting bodies were observed using scanning electron microscopy. The H. erinaceus isolates demonstrated different PCR polymorphisms produced by universal fungal primers (UFPs) and were classified into four groups based on their high genetic diversity.

• 한국균학회소식:학술대회논문집
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• 2014.10a
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• pp.21-21
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• 2014

The importance of utilizing biological resources has become magnified and it has been a big issue to share the benefit among nations as Nagoya Protocol began in 2010. This study was conducted to research the diversity and distribution of wild mushrooms, and to survey the traditional mushroom knowledge of the people in Jejudo which is a volcanic island having a distinctive climate and forest environment. The research sites were Dongbaekdongsan, Keuneonggot, Hallasan National Park, Muryeongarioreum, Saryeonisupgil and other important area where mushrooms are growing spontainously in Jejudo. A total of 511 species comprising 2 phylums, 8 classes, 20 orders and 74 genera were identified from 1600 specimens collected from 2006 to 2012. In previous studies, a total of 561 species comprising 69 families and 99 genera were investigated. As a result, a total of 755 species comprising 23 orders, 87 families and 263 genera were documented in Jejudo. In this study, 137 species were newly identified as unrecorded species in Jejudo and 9 species, Amanita gemmata, Tricholoma aurantiipes, Panellus violaceofulvus, Leucopaxillus septentrionalis, Bondarzewia montana, Psilocybe argentipes, Boedijnopeziza insititia, Sarcoscypha occidentalis for. occidentalis and Morchella patula var. semilibera were the first record for Korea. Also, 7 species, Amanita gemmata, Tricoloma aurantiipes, Panellus violaceofulvus, Leucopaxillus septentrionalis, Boedijnopeziza insititia, Sarcoscypha occidentalis for. occidentalis and Morchella patula var. semilibera were known as only growing in Jejudo. The traditional knowledge was collected from visiting and questionnaire survey in 50 villages in Jejudo. A total of 23 mushrooms were found in which 12 species were used for food, 2 species were poisonous, 6 species were medicinal, 2 species were used for folk religion and 3 species were used for play purposes. Macrolepiota procera was the most commonly used as an edible mushroom and Chlorophyllum neomastoidea was the most well known poisonous mushroom. Also, 267 cases of traditional knowledge about using mushrooms as a food and medicine were collected. This study has significance for supplementing previous studies about distribution of wild mushrooms in Jejudo and documenting unrecorded species in Korea. Also, it is valuable by providing important data of traditional knowledge for using mushrooms since old times.

• The Korean Journal of Mycology
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• v.42 no.2
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• pp.104-132
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• 2014

This study was conducted to survey the diversity and distribution of mushrooms growing spontaneously on Jeju Island in the last eight years. Approximately 2,000 specimens of mushrooms were collected in Gotjawal, Hallasan Natural Park, and Oreum, the habitats where a rich diversity of mushrooms was expected, from 2006 to 2013. They were identified as 74 families, 213 genera and 511 species. Thus, including 69 families, 99 genera and 561 species from the previous study conducted in 2005, a total of 83 families, 257 genera and 740 species of wild mushrooms have been recorded. This study would support new information for fungal flora in Korea and preliminary data for further studies on mushroom diversity.

• The Korean Journal of Mycology
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• v.44 no.4
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• pp.289-295
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• 2016

Lentinula edodes is an edible wild mushroom that can be found in mountainous regions of the Korean peninsula. Wild oak mushrooms were collected from Quercus mongolica at an elevation of more than 1,000 m on Mt. Jungwang and Mt. Gariwang in Gangwon province. We examined 10 oak mushroom strains to evaluate the genetic similarity among strains. Genetic similarity was determined based on the analysis of microsatellite markers (Led A2, Led A8, Led B2, Led B6, and Led D6) registered in the National Center for Biotechnology Information. We also performed dual culture tests on potato dextrose agar for 2 months at $25^{\circ}C$. The observed heterozygosity across all microsatellites ranged from 0.00 and 0.60 among 5 microsatellite markers, and the polymorphism information content values of Led A2, Led A8, Led B2, Led B6, and Led D6 were 0.0000, 0.8144, 0.6194, 0.4892, and 0.5702, respectively (mean value = 0.4987). Confrontation lines between strains were formed for almost all combinations. In conclusion, the oak mushroom populations of Mt. Jungwang and Mt. Gariwang have mixed gene pools. However, further studies are needed to identify genetic similarities and variations among these populations.

• Mycobiology
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• v.38 no.1
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• pp.17-25
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• 2010

The common split-gilled mushroom, Schizophyllum commune is found throughout the world on woody plants. This study was initiated to evaluate conditions for favorable vegetative growth and to determine molecular phylogenetic relationship in twelve different strains of S. commune. A suitable temperature for mycelial growth was obtained at $30^{\circ}C$. This mushroom grew well in acidic conditions and pH 5 was the most favorable. Hamada, glucose peptone, Hennerberg, potato dextrose agar and yeast malt extract were favorable media for growing mycelia, while Lilly and glucose tryptone were unfavorable. Dextrin was the best and lactose was the less effective carbon source. The most suitable nitrogen sources were calcium nitrate, glycine, and potassium nitrate, whereas ammonium phosphate and histidine were the least effective for the mycelial growth of S. commune. The genetic diversity of each strain was investigated in order to identify them. The internal transcribed spacer (ITS) regions of rDNA were amplified using PCR. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 129 to 143 bp and 241 to 243 bp, respectively. The sequence of ITS1 was more variable than that of ITS2, while the 5.8S sequences were identical. A phylogenetic tree of the ITS region sequences indicated that the selected strains were classified into three clusters. The reciprocal homologies of the ITS region sequences ranged from 99 to 100%. The strains were also analyzed by random amplification of polymorphic DNA (RAPD) with 20 arbitrary primers. Twelve primers efficiently amplified the genomic DNA. The number of amplified bands varied depending on the primers used or the strains tested. The average number of polymorphic bands observed per primer was 4.5. The size of polymorphic fragments was obtained in the range of 0.2 to 2.3 kb. These results indicate that the RAPD technique is well suited for detecting the genetic diversity in the S. commune strains tested.

• Journal of Mushroom
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• v.20 no.4
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• pp.208-217
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• 2022

Mushrooms in Naejangsan National Park between May and September of 2021 have been surveyed. In this period, a total of 4 divisions, 9 classes, 25 orders, 72 families, 171 genera, and 381 species, including 3 climate-sensitive biological indicator species were found. The order in which the most diverse array of species was observed is Agaricales, which includes 24 families, 64 genera, and 170 species. Among these, the genus Russula was dominant, with 30 species, followed by the genus Amanita with 27 species. Among the 12 grids we investigated, species diversity was greatest in grid F5, in which 56 species of mushrooms were found. In particular, a large number of ectomycorrhizal mushrooms, including Russula spp. and Lactarius spp. were recognized. We presume that the gentle slopes and the low occurrence of Sasa borealis in this area may create a favorable environment for wild mushrooms. In corroboration, some grids (e.g. F6, F8, and F10) covering steep slopes and harboring large numbers of Sasa borealis contained only 19 species. Based on DNA sequence analysis, the NJ21064 was identified as Chlorophyllum hortense, which is newly recorded in Korea.

• Journal of Mushroom
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• v.21 no.1
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• pp.22-32
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• 2023

To investigate the effect of thinning intensity on environmental factors and ectomycorrhizal mushroom fruiting in forest ecosystems, we studied canopy closure, throughfall, soil temperature, soil moisture, light response of understory vegetation, and ectomycorrhizal mushroom fruiting in a 10-year-old pine forest after 34%, 45%, and 60% thinning. Canopy closure was significantly higher in the 34% treatment and control plots, ranging from 80-85% in April. However, in November, all thinning treatment plots showed a decrease of approximately 5-10% compared with the control plot. The 60% treatment plot had over 200 mm of additional throughfall compared with the control plot, and monthly throughfall was significantly higher by more than 100 mm in October. The soil temperature in each treatment plot increased significantly by up to 1℃ or more compared with the control plot as the thinning rate increased. The soil moisture increased by more than 5% in the thinning treatment plots during rainfall, particularly in the 34% treatment plot, where the rate of moisture decrease was slower. The photosynthetic rate of major tree species (excluding Pinus densiflora)was highest in Quercus mongolica, with a rate of 7 µmolCO₂·m^-2·s^-1. At a lightintensity of 800 μmol·m^-2·s^-1, Q. mongolica showed the highest photosynthetic level of 6 ± 0.3 μmolCO₂·m^-2·s^-1 in the 45% treatment. The photosynthetic rate of Fraxinus sieboldiana and Styrax japonicus increased as the thinning intensity increased. The Shannon-Wiener index of mycorrhizal mushrooms did not significantly differ among treatments, but the fresh weight of mushrooms was approximately 360-840 g higher in the 34% and 45% treatments than in the control. Additionally, the fresh weight of fungi in the 60% treatment was 860 g less than that in the control. There were more individuals of Amanita citrina in the control than in the thinning treatment, while Suillus bovinus numbers increased by more than 10 times in the 34% thinning treatment compared with the control.

• Journal of Mushroom
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• v.3 no.4
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• pp.145-153
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• 2005

Two hundred seventy-four microorganisms were isolated from the soil of abandoned mines around for selecting the heavy metal-degrading strains and their microbial diversity was analyzed. The specimens of macrofungi were isolated from the soil of abandoned mines around for selecting the heavy metal-degrading strains. Eunseong and Dogok in Gyeongsangbuk-do, for selecting the heavy metal-degrading strains. Soil was also collected from same location. Contents of heavy metals (Cd, Cu, Pb, Zn, Ni, Cr, and As) were determined spectrometically in fruiting bodies of forty eight wild macrofungi specimens with soils. When the analysed their transfer factor from soil to fruiting body, Amanita volvata have the highest transfer factor of cadmium and arsenic. And Mycena pura showed the highest in mercury, Marasmius pulcherripes in zinc, Laccaria laccata in nickel, and Collybia confluens in chrome. When compare the population of mushrooms between the contaminated mines and Mt. Chiak as the healthy area, Genus Russula and Collybia were collected both area, but Leucocoprinus, Coprinus, Suillus, Lepiost, Gyroporus, Lepista, Microstoma, Stropharia, and Agrocybe were only in the contaminated mine area.