• Journal of Pharmacopuncture
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• 제16권2호
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• pp.33-40
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• 2013

Objective: This study was conducted to investigate the amino-acid compositions of pharmacopuncture extracts taken from the body and from the tail of Buthus martensii Karsch, which are frequently prescribed in Oriental medicine. Methods: Amino acids in hot water and 70% ethanol extracts taken from the scorpion's whole body and from its tail were screened by using high performance liquid chromatography (HPLC). The experiments were performed with linearity, precision and accuracy. Results: The results of the amino-acid-composition analysis showed that the Buthus martensii Karsch extracts contained various amino acids such as aspartic acid, histidine, alanine, tyrosine, and cystine. The amino-acid analysis showed that the hot water extract was more beneficial than the ethanol extract, except for histidine. The amino acids from the tail and the body of the scorpion were compared, and the concentration of aspartic acid in the extract from the scorpion's tail was two times that found in the extract from its body. The results of validation experiments were all satisfactory. Conclusion: Studies on the ingredients in extracts from a scorpion other than buthotoxin may demonstrate that the antiepileptic efficacy, anticancer activity, anti-thrombotic action and analgesic effect are enhanced. Using only the tail of the scorpion when pharmacopuncture is dispensed may be beneficial because the extracts from the tail of the scorpion have higher potency than those from the whole body.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.85-85
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• 2019

Objective: In this study, the anti-stress effects of extract of Hydrangeae Dulcis Folium (EHDF) or ethalonic extract of Opuntiaficus-indica (EOF) of natural extracts from Jeju Island were investigated. Methods: We performed measurement of whole-body cortisol level and behavioral experiments including the novel tank test (NTT) or the open field test (OFT) to assess stress responses in zebrafish. To induce physical stress, we used the net handling stress (NHS). Fish were treated with EOF or EHDF for 6 min before they were exposed to stress. And then, we sacrificed fish for collecting body fluid from whole-body or conducted behavioural tests, including novel tank test and open field test, were evaluated to observe anxiety-like behaviours and locomotion. We used the cortisol enzyme-linked immunoassay kit to measure the amount of cortisol in each zebrafish sample. Results: The results indicate that increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with both EOF and EHDF (P < 0.05). Moreover, compared with the unstressed group, which was not treated with NHS, the whole-body cortisol level was significantly increased by treatment with NHS. Compared with the NHS-treated stressed control group, pre-treatment with each EHDF and EOF for 6 min significantly prevented the NHS-increased whole-body cortisol level (P < 0.05). Conclusions: In conclusion these results suggest that both EOF and EHDF pretreatment may prevent stress responses and that its mechanism of action may be related to its positive effects on cortisol release.

• YAKHAK HOEJI
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• 제56권1호
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• pp.1-8
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• 2012

In this study, we investigated the anti-obese activity of sevennight extract in high fat diet-ob/ob C57BL/6J mice by oral administered for 1 weeks. Mate water extract (MATEWi) was found to lower whole body and epididymal adipose tissue weights and lowered plasma levels of triglyceride (TG) and total cholesterol (TC), HDL and LDL, compared to those in high fat fed ob/ob group. These results suggest that Mate extract ameliorates obesity through activation of lipogenic enzymes and FA oxidation resulting from phosphorylation of AKT and GSK-$3{\beta}$, and could be developed as a potential therapeutic agent for obese mices.

• International Journal of Human Ecology
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• 제16권2호
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• pp.1-9
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• 2015

This study investigated the effects of persimmon-dyed clothing materials upon thermophysiological responses and subjective comfort sensations during exercise and rest in a warm environment. Six healthy, untrained women participated in two separate testing sessions, with cotton materials dyed with astringent persimmon extract (DC) and undyed cotton materials (UDC). The physical characteristics associated with heat and moisture transfer were improved in DC; also, stiffness, anti-drapery stiffness and crispness in the primary hand values were higher in DC. The experimental protocol consisted of a 10-min rest, 15-min exercise on a treadmill (at ${7km{\cdot}h^{-1}}$) and 25-min recovery at $28{\pm}0.2^{\circ}C$ and $50{\pm}3%\;RH$. The results were as follows: When wearing DC rather than UDC, mean body temperature, heart rate, heat storage and body mass loss were significantly lower during the whole experimental period. Clothing microclimate temperature showed different profiles between the two clothing materials, being lower with DC than UDC during the first half of exercise and the second half of recovery. Clothing microclimate humidity was significantly lower with DC than UDC during the whole experimental period. When wearing UDC, subjects felt significantly warmer and less comfortable during exercise, and sensed greater humidity during exercise and recovery. These results suggest that eco-friendly clothing materials dyed with astringent persimmon extract can reduce exercise-induced heat load and improve subjective sensations when exercising and resting in a warm environment, due to greater heat dissipation from the body to the outside environment compared with undyed clothing materials.

• Korean Journal of Medicinal Crop Science
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• 제25권6호
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• pp.404-410
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• 2017

Background: Hair loss related syndromes are increasing due to environmental pollution and stress. Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanol extracts from the flowers of Calendula officinalis, fruit body of Phellinus linteus, and the whole plant of Houttuynia cordata on the growth of CCD-986 cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells. Methods and Results: All sample extracts at all concentrations, except for that from P. linteus fruit body at $500{\mu}g/m{\ell}$, were cytotoxic to CCD-986 cells. However, none of the sample extracts were cytotoxic to HFDPC. The lipid differentiation of 3T3-L1 cells regulates hair regeneration via secretion of platelet derived growth factor. The 70% ethanol extract of H. cordata whole plant promoted hair growth. Adipogenesis rate significantly increased in a treatment concentration-dependent manner. Conclusions: These results suggest that 70% ethanol extracts of C. officinalis flower, P. linteus fruit body and H. cordata could be used for the development of hair care products.

• The Korea Journal of Herbology
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• 제30권1호
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• pp.43-49
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• 2015

Objectives : In this study, the anti-stress effects of ethanolic extract of Opuntiaficus-indica (OF70E) were investigated. Methods : To determine the effects of OF70E on physical stress, changes in whole-body cortisol level or behavior were monitored in zebrafish. After treatment with 0.9% saline or OF70E for 6 min, all fish underwent net handling stress (NHS), which induced physical stress. And then, we conducted open field test (OFT) or sacrificed fish for collecting body fluid from whole-body. We used the cortisol enzyme-linked immunoassay kit to measure the amount of cortisol in each zebrafish sample. Results : In result, compared with normal group which were not treated by NHS, whole-body cortisol levels were significantly increased in stressed-control group. Compared with control group, pretreatment with OF70E at concentrations of 25, 50 and 100 mg/L for 6min significantly inhibited the increase of whole-body cortisol levels induced by NHS(p<0.05). To anti-stress effects of OF70E on behavior, we conducted OFT after the induction of NHS following pretreatment of OF70E. As results of OFT, compared with unstressed-normal group, distance moved was significantly decreased by induction of NHS in stressed-control group (p<0.05). OF70E-pretreatment blocked decreases of distance moved increased by NHS (p<0.05). And meandering movement, immobility and turn angle were significantly increased by NHS in stressed-control group compared with unstressed-normal group (p<0.05). OF70E-pretreatment prevented the increases of meandering movement immobility and turn angle by NHS (p<0.05). Conclusions : In conclusion these results suggest that OF70E-pretreatment may prevent stress responses.

• Proceedings of the Optical Society of Korea Conference
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• 한국광학회 2006년도 하계학술발표회 논문집
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• pp.351-353
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• 2006

• Korean Journal of Pharmacognosy
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• 제34권4호통권135호
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• pp.339-343
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• 2003

We investigated the effects of Rhus verniciflua Stokes (RVS) on high fat diet- induced obesity in C57BL/6 that received ad libitum exposure to a high fat diet for 8 weeks. Male C57BL/6 mice weighing $20.1{\pm}0.95\;g$ were divided into four groups and the body weight and food intake were weighed for 8 weeks. After the administration of RVS extract, the liver, subcutaneous and retroperitoneal adipose tissue in the mice were weighed and common blood count in whole blood and biochemical parameters in plasma were measured. It was founded that body weight increase was significantly suppressed by the oral administration of RVS extract in group with high fat diet. Weight of subcutaneous adipose tissue and concentration of triglyceride in plasma from mice which were administrated RVS extract were lower than obesity control group. These results suggest that RVS extract may effect on inhibition of diet-induced obesity.

• Korean journal of applied entomology
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• 제34권2호
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• pp.100-105
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• 1995

A whole body extract (WBE), a crude nuclear fraction, of aphids was prepared and used to identify the proteins which bound specifically to 5'-upstream regions of the transcription initiation site of the aphid ribosomal RNA gene (rDNA). While DNA fragment (-263/-195) was bound by only one specific 53 kDa protein, two DNA fragments, A(-194/23) and B(-393/-264), were commonly bound by three proteins (52 kDa, 50 kDa and 40 kDa). It was also revealed that the formation of he DNA-protein complex requires a cation.

• Archives of Pharmacal Research
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• 제13권1호
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• pp.113-116
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• 1990

People in some tropical countries used to use some plant extracts in folklore medicine as treatment of diabetes melitus. Of these plants is Calligonum comsum ("orta"). The present work deals with the biochemical effects of the whole plant water extract given orally to the albino rats, normals and alloxan diabetics, as to fulfil its hypoglycemic effects on rats. The results showed that "orta" extract produced a hypoglycemic effect indicated by the decrease in blood sugar level. It was observed that the diabetic state in rats treated with 300 mg/kg body weight of "orta" extract, was alleviated, showeing normal levels of blood glucose, liver fat and cholesterol contents. Liver proteins were still below the normal level in the rats. Gibenclamide, a hypoglycemic drug, was used for comparison with "ora" extract. Although it did alleviate the diabetic state, yet the liver fat and cholesterol contents were still higher than those of normal. Also the liver proteins were lower than the control levels.