• The Plant Pathology Journal
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• v.30 no.1
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• pp.25-32
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• 2014

Fusarium head blight (FHB; scab) caused mainly by Fusarium graminearum is a devastating disease of wheat and barley around the world. FHB causes yield reductions and contamination of grain with trichothecene mycotoxins such as deoxynivalenol (DON) which are a major health concern for humans and animals. The objective of this research was to develop an easy seed or seedling inoculation assay, and to compare these assays with whole plant resistance of twenty-nine Korean winter wheat cultivars to FHB. The clip-dipping assay consists of cutting off the coleoptiles apex, dipping the coleoptiles apex in conidial suspension, covering in plastic bag for 3 days, and measuring the lengths of lesions 7 days after inoculation. There were significant cultivar differences after inoculation with F. graminearum in seedling relative to the controls. Correlation coefficients between the lesion lengths of clip-dipping inoculation and FHB Type II resistance from adult plants were significant (r=0.45; P<0.05). Results from two other seedling inoculation methods, spraying and pin-point inoculation, were not correlated with adult FHB resistance. Single linear correlation was not significant between seed germination assays (soaking and soak-dry) and FHB resistance (Type I and Type II), respectively. These results showed that clip-dipping inoculation method using F. graminearum may offer a real possibility of simple, rapid, and reliable for the early screening of FHB resistance in wheat.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.2
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• pp.187-195
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• 2008

Polyphenol oxidase (PPO) is implicated in discoloration of white salted noodles and other wheat based foods. PPO activity was evaluated to determine the effect on discoloration of noodle dough sheets prepared from 25 Korean wheat flours during storage and to screen experimental lines with low PPO activity in 52 Korean wheats. PPO activity was assayed with whole-seed and performed with L-dihydroxyphenylalanine (L-DOPA) as substrates. Absorbance by L-DOPA assay of 25 Korean wheats was from 0.285 to 1.368 at 475 nm. PPO activity was significantly related with grain characteristics, including 1000-kernel weight and grain colors. In flour characteristics, PPO activity positively correlated with ash and protein content (r = 0.658, P < 0.001 and r = 0.424, P < 0.05, respectively) and negatively correlated with $L^*$ value of flour (r = 0.412, P < 0.05). In the changes of color of noodle dough sheet, $L^*$ and $b^*$ values consistently decreased and $a^*$ value increased during storage. PPO activity negatively correlated with $L^*$ value of noodle dough sheet during storage (r = 0.566, P < 0.01 at 2 hr, r = 0.547, P < 0.01 at 24 hr, and r = 0.509, P < 0.01 at 48 hr). But, no significant relationship was found in between PPO activity, $a^*$ and $b^*$ values during storage. The 52 Korean wheat lines examined in this study were divided into 3 different groups, low (< 0.500), medium (0.501-0.999) and high level (> 1.000), on the basis of the level of PPO activity. Twenty two Korean wheat lines showed low level of PPO activity and Suwon 252, 277 and 280 showed lower PPO activity (< 0.200) than others.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.1
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• pp.27-37
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• 2014

This study was carried out to characterize the color of noodle sheet made from Korean wheat cultivars, which were then evaluated on grain and flour properties, in order to enhance the breeding-line selection for noodle wheat. In particular, a rapid method was applied to investigate the effects of iron (Fe) contents on the lightness of noodle sheet for the selection of breeding-lines with high Fe contents. Wheat flour lightness of Korean wheat cultivars had a negative correlation with ash (r = -0.634, P<0.01), protein (r = -0.635, P<0.01) and total polyphenols (r = -0.493, P<0.05). The noodle sheet color showed a positive correlation with lightness, but a negative correlation with test weight, 1000 kernels weight, PPO activity and total polyphenol contents. Also, upon the wheat flour properties, the ash, protein and total polyphenol contents had a negative correlation with the lightness of noodle sheet, and this relation varied depending on the year and cultivars. The Fe content had a negative relation with the color of wheat flour and the lightness of noodle sheet, and the potassium hexacyanoferrate(II) trihydrate (PHT) test resulted in blue color. There was no correlation between Fe contents and the color of grains with PHT treatment, but after grounding the whole grains, a negative correlation was found between Fe contents and redness (r = -0.665, P<0.001) and yellowness (r = -0.658, P<0.001) of whole wheat flour with PHT treatment. On the other hand, the lightness of wheat flour with PHT treatment showed a negative relation with Fe content.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.3
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• pp.205-216
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• 2010

Few spring sowing have been conducted on winter cereal crops for whole-crop silage use. Experiments were conducted during 2007 and 2008 at the Chungnam Agricultural Research & Extension Services. The objectives of this study were compared the spring sowing with the optimum season's sowing on growth, yield and feed quality in five winter cereal crops. The treatments consisted of 5 winter cereal crops, Youngyang (Barley, Spring habit I), Keumkang (Wheat, Spring habit II), Gogu(Rye, Spring habit estimated III), Shinyoung (Triticale, Spring habit estimated III), Samhan(Oat, Spring habit estimated II), and 3 planting dates, 18 October (optimum season's sowing), 23 February and 10 March in spring. Heading days as affected by spring sowing compared to optimum season sowing were delayed by 16~20 days in barley, wheat, rye and triticale, and 9 days in oat. The clipping dates at the optimal harvesting stage of each crop for round-baled silage in spring sowing was 8 June (yellow ripe stage) in barley, 25 May (10 days after heading) in rye, and 17 June in wheat (yellow ripe stage), triticale (milky stage) and oat (milky stage). The accumulative temperature from emergence to heading was significantly decreased as affected by spring sowing compared to optimum season's sowing, but that of sowing to emergence and that of heading to maturing was similar. The rate of spikes per tillering surveyed at each clipping date was 62.0-73.1 percent in barley, wheat, triticale and oat, and 56.0 percent in rye compared to that of optimum season sowing. The dry matter yield in spring sowing compared to 18 October was obtained about 71.7 percent in barley, 60.6 percent in wheat, 46.2 percent in rye, 70.2 percent in triticale and 110.9 percent in oat. It were increased in acid detergent fiber (ADF), neutral detergent fiber (NDF) and crude protein content, but decreased in digestible dry matter content(DDM) and relative feed value (RFV). The yield of DDM by spring sowing was decreased in barley, wheat, rye and triticale, but increased in oat. The yield of dry matter and DDM were higher in oat and triticale than that of barley, wheat and oat. So, regardless to clipping dates and cropping system, the appropriated crop for spring sowing was oat, and subsequently triticale and barley. It was not adopted for spring sowing in rye because of low rate of no. of spikes per tillers and yield. It was necessary eliminated winter growing nature by earlier sowing at the late of February after overwinter.

• Fisheries and Aquatic Sciences
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• v.18 no.2
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• pp.159-164
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• 2015

A feeding experiment was conducted to determine the effect of dietary distillers dried grain (DDG) on the growth and body composition of juvenile abalone, Haliotis discus hannai. Three diets were formulated to contain 0% (DDG0), 30% DDG (DDG1) replacing wheat flour and 30% DDG (DDG2) replacing fish meal and wheat flour. A commercial feed (CF) was also compared with experimental diets. Three replicate groups of abalone averaging $0.6{\pm}0.01g$ were fed one of the four diets for 14 weeks. The highest survival rate was observed in the abalone fed the DDG2 diet, which was higher than that of abalone fed the DDG0 diet. Weight gain, shell length, shell width and soft body weight of the abalone were not affected by dietary DDG and CF diet (P > 0.05). Proximate and amino acid composition of the soft whole body were not affected by dietary DDG and CF diet (P > 0.05). The result of this experiment suggests that DDG is a good ingredient to replace fish meal and wheat flour in the diet and could be used up to 30% in the diet without negative effects on the growth performance of juvenile abalone Haliotis discus hannai.

• Proceedings of the Korean Society of Life Science Conference
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• 2003.10a
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• pp.14-27
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• 2003

The genomes of Arabidopsis and rice have been fully sequenced. Genomic sequencing provides global information about genome structure and organization. A comprehensive research account of our recent studies conducted on genome painting, comparative genomics and genome fusion is provided in order to project the prospects of plant cytogenetic research in post-genomics era. Genome analysis by GISH using genome painting is demonstrated as an excellent means suitable for visualization of a whole genome, since total genomic DNA representing the overall molecular composition of the genome is used as a probe. FISH on extended DNA fibers has been developed for high-resolution FISH and has contributed to determining the copy number and order of genes. We have also mapped a number of genes involving starch synthesis on wheat chromosomes by FISH and compared the position of these genes on linkage map of rice. Macro synteny between wheat and rice can be observed by comparing the location of these genes in spite of the fact that the size of DNA per chromosome differs by 20 fold in two. Moreover, to approach our goal towards making bread and udon noodles from rice flour in future by incorporating bread making and the noodle qualifies in rice, we have been successful in introducing large genomic DNA fragments containing agronomically important genes of wheat into a rice by successive introduction of large insert BAC clones, there by expanding genetic variability in rice. We call this method genome fusion.

• Journal of Nutrition and Health
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• v.18 no.3
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• pp.234-241
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• 1985

The purpose of this study was to investigate changes in nutritive quality of fermented wheat bran prepared by culturing with a microorganism, Aspergillus oryzae, in an attempt to improve the quality of protein in feedstuff. After incubation of wheat bran with Aspergillus oryzae, the contents of chemical composition, including amino acids, riboflavin and amino-nitrogen were increased, but the level of nitrogen free extract was reduced. The effects of supplementation of fermented wheat bran on the rat diets were evaluated by measuring growth rate, feed efficiency and biological values, such as NPU, PER and NPR. Sixty four male Sprague Dawley rats of 5-6 weeks of age were adopted for the feeding trial for 10 days period, and levels of dietary protein were set at 10%. The %contribution of protein from casein, wheat bran and fermented wheat bran for the other dietary treatments were, in the order, 50-50-0%, 50-30-20%, 50-20-30% and 50-0-50%, respectively. In the result of this study, no significant difference were observed in the amount of feed intake body weight gain and feed efficiency. On the whole, the values of NPU, PER and NPR of all the fermented wheat bran groups appeared to be higher than those of the group whose half of the protein was from wheat bran.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.9
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• pp.1463-1468
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• 2004

This study was conducted to examine the effects of feeding diets containing bioorganic power (BP) flour, wheat flour and rice flour on blood glucose and lipid level in streptozotocin-induced diabetic rats. BP flour was composed with the mixture of defatted flour (16 g soybean, 12 g wheat, 8 g barley), 2 g red ginseng and 2 g whole wheat flour per 100 g diet. Experimental groups were divided into non-diabetic normal and 4 diabetic groups containing control, 40% BP, 41.6% wheat and 41.6% rice flours with basal diet. BP group was significantly increased body weight gain and decreased plasma glucose compared with the diabetic control, wheat and rice flour groups (p<0.05). Food efficiency ratio of diabetic rats were significantly lower than that of the normal rats. The concentrations of total cholesterol and triglyceride in plasma and atherogenic index were significantly decreased in BP, wheat and rice diabetic groups than diabetic control (p<0.05). The concentration of total cholesterol and triglyceride in liver was significantly lower in BP group as compared with the diabetic control, wheat and rice flour groups (p<0.05). In conclusion, the results indicated that BP flour feeding improved plasma glucose level, total cholesterol and triglyceride in diabetic rats.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.10
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• pp.1307-1313
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• 2007

In order to evaluate the utilization of immature green wheat kernels as food processing material, these experiments were carried out to analyze general composition, free sugars, free amino acids, mineral content and color of 3 wheat varieties according to their heading dates. Contents such as moisture, crude protein, crude lipid and ash were gradually decreased according to the days after heading of 3 wheat varieties, while crude fiber and starch were gradually increased. Maltose and maltotriose contents in immature green wheat kernels were high but its rapidity decreased by closing to ripeness. Contents of free amino acids showed a tendency to decrease, among which alanine content was the highest around 25 days after heading, and then glutamic acid, GABA, glycine in order. Contents of total free amino acid were decreased by closing to ripeness. Among 3 wheat varieties, its contents of Guru wheat was the highest. As wheat matured, ${\beta}-glucan$ contents were decreased from 1.5% to 0.28% on 43 days after heading. Mineral contents of 3 wheat varieties were generally increased by closing to ripeness after coming into ears, and K, Mg were especially prominent. As wheat matured, lightness was decreased, while redness and yellowness were increased. Considering chemical composition and color, the immature green wheat were produced through blanching the spikes harvested before the yellow ripe stage. The harvested green wheat cereals is able to eat raw wheat or cook it as food processing material.

• Applied Biological Chemistry
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• v.35 no.2
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• pp.92-98
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• 1992

Some physicochemical properties and molecular structure of cow pea, mung bean and acorn starches(mook-forming starches) and red bean, wheat and sweat potato starches(mook-unforming starches) were investigated. Amylose contents of cow pea, mung bean and acorn starch were higher than the others. Cow pea starch was similiar to mung bean starch in gelatinization characteristics by Brabender amylogram but cold viscosity of red bean starch and peak viscosity of sweet potato starch were especially high. Whereas viscosity of wheat starch was low in whole temperature range. Amylose molecules of larger molecular size$(above\;5{\times}10^5\;molecular\;weight)$ of three mook-forming starches were more than shoes of red bean and wheat starch. Chain distribution ratios$(DP\;35{\sim}55\;to\;DP\;10{\sim}20)$ of cow pea, mung bean and acorn amylopectin were higher than thoes of red bean, sweet potato and wheat amylopectin.