• The Plant Pathology Journal
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• 제33권3호
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• pp.345-349
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• 2017

Tomato chlorosis virus (ToCV), a member of the genus Crinivirus, has caused an epidemic disease in tomato worldwide. ToCV is phloem-limited and transmitted by whiteflies in a semi-persistent manner, but not by mechanical inoculation. Experimental propagation of ToCV has been performed primarily by using whitefly-mediated inoculation. To develop a simple and convenient method for transmission of ToCV, we investigated grafting single-leaflets from tomato plants infected with ToCV to recipient tomato seedlings. Forty-one of 46 tomato seedlings tested were grafted successfully with single-leaflets infected with ToCV. Among them, 36 seedlings (87.8%) were systemically infected with ToCV and developed typical symptoms. Our results demonstrated that single-leaflet grafting could provide a sufficient amount of inoculum for the transmission of ToCV to the grafted seedlings.

• Mycobiology
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• 제46권4호
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• pp.440-447
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• 2018

Dual biocontrol of both insects and plant pathogens has been reported for certain fungal entomopathogens, including Beauveria bassiana and Lecanicillum spp. In this study, we demonstrate, for the first time, the dual biocontrol potential of two fungal isolates identified by morphological and phylogenetic analyses as Isaria javanica. Both these isolates caused mortality in the greater wax moth, and hence can be considered entomopathogens. Spores of the isolates were also pathogenic to nymphs of the green peach aphid (Myzus persicae), with an $LC_{50}$ value of $10^7spores/mL$ 4 days after inoculation and an $LT_{50}$ of 4.2 days with a dose of $10^8spores/mL$. In vitro antifungal assays also demonstrated a strong inhibitory effect on the growth of two fungi that are pathogenic to peppers, Colletotrichum gloeosporioides and Phytophthora capsici. These results indicate that I. javanica isolates could be used as novel biocontrol agents for the simultaneous control of aphids and fungal diseases, such as anthracnose and Phytophthora blight, in an integrated pest management framework for red pepper.

• 한국응용곤충학회지
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• 제63권1호
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• pp.75-76
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• 2024

몇 종의 주요 농작물 해충에 대한 식물추출물(cinnamon extract 5% + citronella oil 10% + citrus oil 30% + derris extract 10% + neem extract 20% + penetrating surfactant 25%)의 살란 효과를 분무법으로 검정하였다. 노린재류의 경우 알이 부화하는 과정에서 치사하는 경향을 보였다. 식물추출물(500배액) 처리시 썩덩나무노린재(Halyomorpha halys), 톱다리개미허리노린재(Riptortus clavatus), 홍비단노린재(Eurydema dominulus), 온실가루이(Trialeurodes vaprarorium), 담배가루이(Bemisia tabaci), 파밤나방(Spodoptera exigua), 검거세미밤나방(Agrotis ipsilon)에 대한 최종 살란 효과는 100%로 높게 나타나, 향후 산업화가 가능할 것으로 생각된다.

• The Plant Pathology Journal
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• 제18권4호
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• pp.179-186
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• 2002

Four newly developed cotton mutants (M-111, M-7662, M-358 and M-218) were compared for their resistance against Cotton leaf curl virus(CLCuV) together with commercial resistant (CIM-443, CIM-482, CIM-473, FH-900 and FH-901) and susceptible (5-12) varieties by artificial inoculation through grafting and under natural field conditions. Infectivity and success of grafting were 100% in all cases. None of the grafted plants were found immune or asymptomatic. All the grafted mutants and most of their single plant progeny rows (SPPRs) showed highly resistant responses as the symptoms displayed by these mutants were milder than the commercial cultivars. Grafted mutants also had delayed disease reactions as they took more time (25-30 days) to produce disease symptoms, as compared with resistant commercial varieties that produced disease 18-22 days after inoculation. Growth of the grafted SPPRs of tested mutants was normal, which is an indication that there will be no production losses. Observations under natural infestation of whitefly showed that two SPPRs of M-ll/CE and M-7662-1/2 and one resistant variety CIM-443 exhibited slight incidence of disease, while one SPPR of M-l1/59 and S-12 were moderately susceptible and highly susceptible with 21％ and 97.l％ disease incidence, respectively. This study also showed that plants displaying more disease symptoms through grafting were easily infected under natural conditions. These results suggest that preference should be given to those plants that exhibited highly resistant responses after artificial inoculation.

• 농업과학연구
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• 제24권1호
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• pp.21-28
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• 1997

전국 일원의 시설 재배 농가 중 353 농가를 방문하여 해충 및 천적의 분포를 조사한 결과 전체 조사 농가의 73%에서 총 52종의 해충이 발생되고 있었으며 이 중 꽃노랑총채벌레, 점박이응애, 온실가루이, 목화진딧물, 복숭아혹진딧물 등이 주요 해충으로 조사되었다. 천적의 발생은 아주 미미하였으나 방문 농가의 11%인 40개소의 시설재배 농사에서 확인하였으며 이는 해충이 발생한 농가의 약 17%에서 볼 수 있었다. 주요 천적으로는 전체 조사 천적 중 진디벌류가 63%, 무당벌레류가 20%, 애꽃노린재류가 8%를 차지하였으며 이들 이외에도 꽃등에류, 포식응애류, 풀잠자리류, 좀벌류, 혹파리류가 채집되었다. 또한 주요 작물별 해충과 천적의 발생정도를 살펴본 결과 토마토에서는 온실가루이, 오이에서는 꽃노랑총채벌레, 목화진딧물, 참외 및 수박에서는 꽃노랑총채벌레와 응애류, 고추에서는 복숭아혹진딧물과 꽃노랑총채벌레, 딸기에서는 응애류와 진딧물류 등이 주요해충으로 조사되었다.

• The Plant Pathology Journal
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• 제34권3호
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• pp.199-207
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• 2018

Tomato chlorosis virus (ToCV) is a whitefly-transmitted and phloem-limited crinivirus. In 2013, severe interveinal chlorosis and bronzing on tomato leaves, known symptoms of ToCV infection, were observed in greenhouses in Korea. To identify ToCV infection in symptomatic tomato plants, RT-PCR with ToCV-specific primers was performed on leaf samples collected from 11 tomato cultivating areas where ToCV-like symptoms were observed in 2013 and 2014. About half of samples (45.18%) were confirmed as ToCV-infected, and the complete genome of 10 different isolates were characterized. This is the first report of ToCV occurring in Korea. The phylogenetic relationship and genetic variation among ToCV isolates from Korea and other countries were also analysed. When RNA1 and RNA2 are analysed separately, ToCV isolates were clustered into three groups in phylogenetic trees, and ToCV Korean isolates were confirmed to belong to two groups, which were geographically separated. These results suggested that Korean ToCV isolates originated from two independent origins. However, the RNA1 and RNA2 sequences of the Yeonggwang isolate were confirmed to belong to different groups, which indicated that ToCV RNA1 and RNA2 originated from two different origins and were reassorted in Yeonggwang, which is the intermediate point of two geographically separated groups.

• The Plant Pathology Journal
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• 제18권5호
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• pp.231-236
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• 2002

Bemisia tabaci Genn. is an important pest worldwide because of its ability to cause damage by direct feeding and its role as a vector of some viruses including geminiviruses. The first report of Tobacco leaf curl virus (TLCV), a Geminiviruses, in Indonesia was in 1932 when the virus was found infecting tobacco plants in Central Java. The characteristic symptoms of TLCV included upward curling of the leaf edge, vein thickening, and sometimes the occurrence of enation on the underside of the leaves. Basic studies were carried out to elucidate the characteristics of TLCV transmission by its vector, B. tabaci. A single whitefly was able to transmit the virus and the efficiency of transmission was increased when the number of adult whiteflies was increased up to 20 per plant. Inoculation access period of 1 h could cause transmission up to 20% and the optimum inoculation access period was 12 h. Acquisition access period of 30 minutes resulted in 70% transmission while 1(10% transmission occurred with a 24-h acqui-sition access period. The virus was proven to be persistently but not transovarially transmitted. Discrete fragments of 1.6 kb were observed when polymerase chain reaction method was applied to detect the virus in viruliferous nymphs and individual adults of B. tabaci, while no bands were obtained from non-viruliferous nymphs and adults.

• 식물병연구
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• 제12권1호
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• pp.1-4
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• 2006

2001년 이후 2005년까지 북측의 씨감자 생산은 남북 협력으로 순조롭게 진행되어 일부는 이미 3급종자(G3)까지 공급되어 증식되고 있는 실정이다. 특히 씨감자 생산 체계의 상위단계인 조직 배양과 양액씨감자(G0) 생산에서는 성공적이었으며, 다음 단계인 1급(G1)$\sim$4급종자(G4)를 어떻게 무병, 격리 조건에서 감염율을 적게 유지, 증식, 관리하느냐에 달려 있다고 해도 과언이 아닐 것이다. 벌써 상위단게 씨감자 생산의 효과가 나타나기 시작하고 있어, 특히 남북한의 지속적인 협력이 절실하다고 할 수 있다.

• 한국응용곤충학회지
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• 제39권1호
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• pp.5-12
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• 2000

임의증폭 다형 DNA(RAPD)와 미토콘드리아 12S, 16S rRNA 유전자의 제한단편 DNA 표식자에 의해 한국에서 발생하는 담배가루이 개체군들의 biotype을 판별하였다. 진천의 장미 온실과 서울 내곡동의 포인세티아 온실에서 발생한 담배가루이는 일본, 이스라엘, 호주의 B biotype 과 동일한 DNA 단편들을 보유하였다. 여러 지역의 노지 콩 (Glycine max), 고구마 (Ipomea batatas), 들깨 (Perilla frutescens)에서 채집된 담배가루이 개체군들은 일본 시코쿠의 인동덩굴(Lonicera japonica)에서 채집된 담배가루이와 같은 DNA로 표식되었다. 이들 non-B biotype은 한국, 일본 등 극동아시아 지역에 고유한 계통으로 보인다. 최근 한국에서 발견된 담배가루이 Bemisia tabaci(Gennadius)의 주사전자현미경 관찰에 의한 형태적 특정을 온실 원예작물의 주요해충인 온실가루이 Trialeurodes vaporariorum (Westwood)와 비교하여 기재하였다.

• 한국응용곤충학회지
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• 제37권2호
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• pp.117-121
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• 1998

온실가루이의 발생량은 성충과 약충 모두 토마토보다 오이에서 많았다. 끈끈이 트랩에 온실가루이는 오이에 접종후 3일째부터 유인되기 시작하여 3회의 발생최성기를 보였으며 온실가루이좀벌은 접종 15일째부터 성충이 유인되었다. 온실가루이 성충은 오이잎의 최상위 부분에 주로 서식하고 있었으며 약충은 초기에는 상위부분에서, 40일째 이후에는 하위부분에서 주로 발생하였다. 온실가루이좀벌 성충은 중상위 부분에서 많이 발생하였으나 부위별 차이는 거의 없었으며, 온실가루이좀벌에 의한 온실가루이 mummy는 오이의 중하위부분에서 발생이 많았다. 온실가루이의 방제를 위하여 온실가루이좀벌을 방사하는 시기는 황색끈끈이 트랩에 온실가루이 성충이 1주일 동안 5마리 정도 잡히기 시작할 때 온실가루 이좀벌mummy를 오이 5주당 50마리(1tag)씩 방사한 경우 방사후 40일정도에 방제효과가 인정되었다.