• 식물병연구
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• 제13권1호
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• pp.50-52
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• 2007

2004과 2005년 2년간 경남농업기술원 함양 약초 전시포장에 재배중인 윤판나물에서 S. rolfsii에 의한 흰비단병이 발생되었다. 병징은 줄기와 지제부위가 수침상으로 물러지고 부패하면서 암갈색으로 되고 시들어 말라 죽는다. 병반부와 지제부위의 토양 표면에 흰색의 곰팡이가 솜털처럼 생기고 감자한천 배지에서 갈색의 둥근 균핵을 많이 형성하였으며 크기는 1$\sim$3mm였다. 균사의 폭은 $3{\sim}10{\mu}m$이며 균사생육 적온은 30$^{\circ}C$였으며 균사특유의 clamp connection이 관찰되었다. 윤판나물에서 발생한 병징과 균학적 특징 및 병원성을 검정한 결과, 이 병을 Sclerotium rolfsii Saccardo에 의한 윤판나물 흰비단병으로 명명하고자 한다.

• 식물병연구
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• 제13권1호
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• pp.53-56
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• 2007

2004년과 2005년 2년간 경남농업기술원 시험포장내 울타리 부근에 재배중인 호박 과실에서 S. rolfsii에 의한 호박 흰비단병이 발생되었다. 병징은 과실이 수침상으로 물러지고 부패하면서 병반부위에 흰색의 곰팡이가 솜털처럼 생기고 갈색의 둥근 균핵을 많이 형성하였으며 크기는 1$\sim$3 mm였다. 이병과실이 지표면과 닿은 부위에도 흰색 균사와 갈색 균핵을 형성하였다 균사의 폭은 $7{\sim}8{\mu}m$이며, 균사생육적온과 균핵 형성은 30$^{\circ}C$에서 가장 좋았으며, 균사특유의 clamp connection이 관찰되었다. 호박 과실에서 발생한 병징과 균학적 특징 및 병원성을 검정한 결과, 이 병을 Sclerotium rolfsii Saccardo에 의한 호박 흰비단병으로 명명하고자 한다.

• 한국균학회지
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• 제41권3호
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• pp.197-199
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• 2013

2011년 7월 경상남도농업기술원 시험포장에서 S. rolfsii에 의한 옥수수 흰비단병 증상이 발생되었다. 병징은 줄기가 수침상으로 물러지고 갈색으로 변하면서 시들어 말라 죽는다. 병반부와 토양 표면에 흰색의 곰팡이가 발생하며 갈색의 작은 둥근 균핵이 형성되었다. 감자한천배지에서 균총은 흰색이고 잘 자라며 갈색의 작은 둥근 균핵을 많이 형성하였다. 균핵의 크기는 1~3 mm이며 균사의 폭은 $4{\sim}8{\mu}m$였다. 균사생육과 균핵형성 적온은 $30^{\circ}C$이었다. 균사 특유의 clamp connection이 관찰되었다. 옥수수에서 발생한 병징과 병원균의 균학적 특징을 조사한 결과, 이 병을 Sclerotium rolfsii Saccardo에 의한 옥수수 흰비단병으로 명명하고자 제안한다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.92.1-92
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• 2003

In order to develop an effective spray program for control of apple white rot with reduced use of fungicides, the control efficacy of several fungicides that has been intensively used for control of the disease was assessed. They were sprayed on the same tree with 15 day interval from late May to early August. Just prior to and after each spray, 100 fruits were bagged with two layered fruit bag to limit the chemical application in only one time, and the disease incidence and latent infection frequency on the bagged apples were examined. Some fungicides such as folpet, iminoctadine-triacetate and azoxystrobin showed a high post-infectional activity even though the former two are non- systemic. Folpet suppressed symptom development, iminoctadine-triacetate reduced infection frequency and azoxystrobin acted in both ways. When those fungicides were ！！ adopted in a spray program, once in a cropping season, their post-infectional activity became much greater. This activity shown by the non-systemic fungicides was supposed to be derived from the peculiar infection process of the white rot fungus of which the pathogen is usually remain latent in the corked cells of lenticel until the apple reach mature stage.

• 한국미생물·생명공학회지
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• 제25권5호
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• pp.527-533
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• 1997

From apple skin, we isolated a bacterial strain which strongly inhibited the growth of apple white rot fungus, Botryosphaeria dothidea. The isolated strain, designated as SS279, was identified to be the genus Bacillus. The antifungal activity of Bacillus sp. SS279 was found in the culture filtrate. The production of antifungal substances occurred during logarithmic phase and was the highest when cultures reached the stationary growth phase. The optimum ranges of temperature and pH for its production were 25-30$\circ$C and 4.5-9.0, respectively. The culture filtrate of Bacillus sp. SS279 exhibited a strong inhibitory effect on the spore germination and germ tube elongation of B. dothidea. Autoclaved culture filtrate of Bacillus sp. SS279 showed only a slight decrease in antifungal activity, indicating that the Bacillus sp. SS279 produce heat-stable antifungal substances. In in vivo bioassay, Bacillus sp. SS279 also showed antagonistic activity against apple white rot caused by B. dothidea.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1147-1151
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• 2007

Biodegradation of endocrine-disrupting bisphenol A was investigated with several white rot fungi (Irpex lacteus, Trametes versicolor, Ganoderma lucidum, Polyporellus brumalis, Pleurotus eryngii, Schizophyllum commune) isolated in Korea and two transformants of T. versicolor (strains MrP 1 and MrP 13). I. lacteus degraded 99.4% of 50 mg/l bisphenol A in 3 h incubation and 100% in 12 h incubation. which was the highest degradation rate among the fungal strains tested. T. versicolor degraded 98.2% of 50 mg/l bisphenol A in 12 h incubation. Unexpectedly, the transformant of the Mn-repressed peroxidase gene of T. versicolor, strain MrP 1, degraded 76.5% of 50 mg/l bisphenol A in 12 h incubation, which was a lower degradation rate than wild-type T. versicolor. The removal of bisphenol A by I. lacteus occurred mainly by biodegradation rather than adsorption. Optimum carbon sources for biodegradation of bisphenol A by I. lacteus were glucose and starch, and optimum nitrogen sources were yeast extract and tryptone in a minimal salts medium; however, bisphenol A degradation was higher in nutrient-rich YMG medium than that in a minimal salts medium. The initial degradation of endocrine disruptors was accompanied by the activities of manganese peroxidase and laccase in the culture of I. lacteus.

• 환경생물
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• 제34권3호
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• pp.201-207
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• 2016

The hazards associated with the polycyclic aromatic hydrocarbons (PAHs) are known to be recalcitrant by their structure, but white rot fungi are capable of degrading recalcitrant organic compounds. Phlebia brevispora KUC9045 isolated from Korea was investigated its efficiency of degradation of four PAHs, such as phenanthrene, anthracne, fluoranthene, and pyrene. And the species secreted extracellular laccase and MnP (Manganese dependent peroxidase) during degradation. P. brevispora KUC9045 demonstrated effective degradation rates of phenanthrene (66.3%), anthracene (67.4%), fluoranthene (61.6%), and pyrene (63.3%), respectively. For enhancement of degradation rates of PAHs by the species, Remazol Brilliant Blue R (RBBR) was preferentially supplemented to induce ligninolytic enzymes. The biodegradation rates of the three PAHs including phenanthrene, fluoranthene, and pyrene were improved as higher concentration of Remazol Brilliant Blue R was supplemented. However, anthracene was degraded with the highest rate among four PAHs after two weeks of the incubation without RBBR addition. According to the previous study, RBBR can be clearly decolorized by P. brevispora KUC9045. Hence, the present study demonstrates simultaneous degradation of dye and PAHs by the white rot fungus. And it is considered that the ligninolytic enzymes are closely related with the degradation. In addition, it indicated that dye waste water might be used to induce ligninolytic enzymes for effective degradation of PAHs.

• Journal of Microbiology
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• 제43권6호
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• pp.503-509
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• 2005

The production of manganese peroxidase (MnP) by Irpex lacteus, purified to electrophoretic homogeneity by acetone precipitation, HiPrep Q and HiPrep Sephacryl S-200 chromatography, was shown to correlate with the decolorization of textile industry wastewater. The MnP was purified 11.0-fold, with an overall yield of $24.3\%$. The molecular mass of the native enzyme, as determined by gel filtration chromatography, was about 53 kDa. The enzyme was shown to have a molecular mass of 53.2 and 38.3 kDa on SDS-PAGE and MALDI-TOF mass spectrometry, respectively, and an isoelectric point of about 3.7. The enzyme was optimally active at pH 6.0 and between 30 and $40^{\circ}C$. The enzyme efficiently catalyzed the decolorization of various artificial dyes and oxidized Mn (II) to Mn (III) in the presence of $H_2O_2$. The absorption spectrum of the enzyme exhibited maxima at 407, 500, and 640 nm. The amino acid sequence of the three tryptic peptides was analyzed by ESI Q- TOF MS/MS spectrometry, and showed low similarity to those of the extracellular peroxidases of other white-rot basidiomycetes.

• Journal of the Korean Wood Science and Technology
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• 제32권6호
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• pp.14-22
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• 2004

The effect of cadmium ions on ligninolytic and decolourizing activities in cultures of two white-rot fungi, Cerrena unicolor and Trametes versicolor, were examined. Cadmium was added to the shallow stationary cultures growing on a liquid mineral medium. Both examined strains sorbed Cd ions in the first 24 hr of incubation. An appreciable stimulation of the activity of extracellular laccase (LAC) and inhibition of the extracellular manganese-dependent peroxidase (MnP) were simultaneously observed when 25 mgL^-1 and 50 mgL^-1 of cadmium ions were added to the cultures. On the other hand, the addition of cadmium ions also resulted in stimulating the decolorization activity of C. unicolor to decolorize Remazol Brilliant Blue R (RBBR) in the cultures, but decreasing it in the culture of T. versicolor, which is compared to the inhibition of MnP activity in this fungus. Our data indicate that the presence of Cd(II) ions can affect the ligninolytic activity of white-rot fungi. It was found that C. unicolor is a strain resistant to the presence of Cd ions in the liquid culture media, and has a potential to use this strain for bioremediation of sites contaminated with both heavy metals and aromatic pollutants.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.704-708
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• 2001

The effect of nutrient nitrogen on the degradation of pentachlorophenol (PCP) by Phanerochaete chrysosporium in a liquid culture was investigated. PCP disappeared at almost the same rate in both nutrient nitrogen-sufficient (NS) and -limited (NL) sttionary cultures. However, more pentachloroanisole (PCA) was accumulated in the NS culture than in the NL culture. The effect of nitrogen on the degradation of PCA was also tested in both cultures. PCA disappeared faster in the NL culture than in the NS culture, indicating that the lower accumulation of PCA during the degradation of PCP in the NL culture was due to the faster degradation of PCA in the NL culture than in the NS culture. In another experiment, PCA was added to shaking cultures rather than stationary cultures to search for any other metabolite(s). While no other metabolite but PCA was found in the NS stationary culture, 2,4,5,6-tetrachloro-2,5-cyclohexadiene-1,4-dione(TCHD) was found as the only indentifiable product in the NL shaking culture. Thus, PCP would appear to be metabolized to TCHD via PCa or directly oxidized to TCHD by lignin peroxidase. Since all the above results indicate that no innocuous metabolite was formed during the degradation of PCP by the fungus, it is quite feasible to use the fungus in the biotreatment of PCP.