• Journal of the Korean Wood Science and Technology
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• 제38권6호
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• pp.568-578
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• 2010

본 연구에서는 다양한 목재부후균의 polychlorinated biphenyls (PCBs) 생분해 가능성을 구명하고자 7종의 백색부후균을 이용하여 PCB 혼합물 중 가장 분해가 어렵다고 알려진 Aroclor 1254와 1260을 대상으로 분해력이 우수한 목재부후균을 선발하였다. 그리고 선발된 목재부후균을 이용하여 GC 분석을 통해 Aroclor 1254, 1260의 분해율을 조사하였다. 고체배지 저항성 테스트를 통해 우수분해 균주로 선발된 Cystidodontia isubellina 는 다른 균주에 비해 균사생장량의 억제가 가장 적었으며 상대적으로 생장속도도 빠른 특징을 나타냈다. 선발된 C. isubellina에 의한 Aroclor 1254와 1260의 분해율 분석 결과, Aroclor 1254배양 13일째 57.57%로 가장 높은 분해율을 나타냈다. Aroclor 1260 역시 배양일수가 증가함에 따라 분해율 역시 증가하는 경향을 보였으며, 배양 13일째 49.43%로, 두 혼합물 모두 기존의 다른 연구에서보다 높은 분해율을 나타냈다.

• Journal of Microbiology
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• 제42권1호
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• pp.37-41
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• 2004

The textile industry wastewater has been decolorized efficiently by the white rot fungus, Irpex lacteus, without adding any chemicals. The degree of the decolorization of the dye effluent by shaking or stationary cultures is 59 and 93%, respectively, on the 8th day. The higher level of manganese-dependent peroxidase (MnP) and non-specific peroxidase (NsP) was detected in stationary cultures than in the cultures shaken. Laccase activities were equivalent in both cultures and its level was not affected significantly by the culture duration. Neither lignin peroxidase (LiP) nor Remazol Brilliant Blue R oxidase (RBBR ox) was detected in both cultures. The absorbance of the dye effluent was significantly decreased by the stationary culture filtrate of 7 days in the absence of Mn (II) and veratryl alcohol. In the stationary culture filtrate, three or more additional peroxidase bands were detected by the zymogram analysis.

• 미생물학회지
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• 제38권4호
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• pp.250-250
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• 2002

This study confirmed that white rot fungus Irpex lacteus was able to metabolize 2,4,6-trinitrotoluene (TNT) with two different initial transformations. In one metabolic pathway of TNT a nitro group was removed from the aromatic ring of TNT. Hydride-Meisenheimer complexes of TNT (H/sup -/-TNT), colored dark redo were confirmed as the intermediate in this transformation by comparison with the synthetic compounds. 2,4-Dinitrotoluene as a following metabolic product was detected, and nitrite produced by denitration of $H^-$-TNT supported this transformation. In the other TNT pathway, nitro groups in TNT were successively reduced to amino groups via hydroxylamines. Hydroxylamino-dinitrotoluenes and amino-dinitrotoluenes were identified as the intermediates. The activity of a membrane-associated aromatic nitroreductase was detected in the cell-free extract of I. lacteus. This enzyme catalyzed the nitro group reduction of TNT with NADPH as a cofactor, Enzyme activity was not observed in the presence of molecular oxygen.

• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.292-295
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• 2002

A chromate-resistant white rot fungus, Inonotus cuticularis, abundant in oak trees, was isolated for chromate removal and detoxification of chromate. Inonotus cuticularis was also investigated for an optimal waste treatment system. The screened cells were able to reduce an initial chromate concentration of as high as 1,300 ppm. Cell growth kinetics showed that the optimum culture conditions in flasks were at $33^{\circ}C$ and pH 4.2. Furthermore, the cells were able to remove $54\%$ of the initial chromate by a two-stage operation based on the combination of a fermentor and airlift reactor.

• Journal of Microbiology
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• 제38권4호
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• pp.250-254
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• 2000

This study confirmed that white rot fungus Irpex lacteus was able to metabolize 2,4,6-trinitrotoluene (TNT) with two different initial transformations. In one metabolic pathway of TNT a nitro group was removed from the aromatic ring of TNT. Hydride-Meisenheimer complexes of TNT (H$\^$-/-TNT), colored dark redo were confirmed as the intermediate in this transformation by comparison with the synthetic compounds. 2,4-Dinitrotoluene as a following metabolic product was detected, and nitrite produced by denitration of H$\^$-/-TNT supported this transformation. In the other TNT pathway, nitro groups in TNT were successively reduced to amino groups via hydroxylamines. Hydroxylamino-dinitrotoluenes and amino-dinitrotoluenes were identified as the intermediates. The activity of a membrane-associated aromatic nitroreductase was detected in the cell-free extract of I. lacteus. This enzyme catalyzed the nitro group reduction of TNT with NADPH as a cofactor, Enzyme activity was not observed in the presence of molecular oxygen.

• 한국식물병리학회지
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• 제14권1호
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• pp.99-101
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• 1998

A severe brown rot on peach fruit caused by a Phytophthora sp. has occurred at peach orchards in Taegu of Korea from late June to early August in 1997. Infected fruits showed irregularly round or circular water soaking brown regions. In the severe case, fruits were entirely rotten and surface of the fruits were wrinkled. Occasionally, white mycelia and abundant sporangia were developed on the surface of fruit. Inner tissues of the fruits were also discolored to brown. The causal fungus was identified as Phytophthora cactorum based on following characteristics. Sporangia were ovoid, conspicuously papillate, caducous and measured as 28.4~48.1$\times$21.9~37.2 (av. 39.9$\times$30.4) ${\mu}{\textrm}{m}$. Sexuality of the fungus was homothallic. Oogonia were 25.0~34.0 (av. 29.9) ${\mu}{\textrm}{m}$ in size. Most antheridia were paragynous and measured av. 10.5$\times$13.0 ${\mu}{\textrm}{m}$. Optimum temperature for mycelia growth was around 25~3$0^{\circ}C$. However none of the isolates grew under 7$^{\circ}C$ and over 35$^{\circ}C$. The fungus revealed high pathogenicity to fruits, shoots and leaves of peach, apple and pear with different degrees. Phytophthora fruit rot of peach caused by Phytophthora cactorum has not been reported in Korea previously.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.800-805
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• 2007

Two endoglucanases with processive cellulase activities, produced from Fomitopsis palustris grown on 2% microcrystalline cellulose(Avicel), were purified to homogeneity by anion-exchange and gel filtration column chromatography systems. SDS-PAGE analysis indicated that the molecular masses of the purified enzymes were 47 kDa and 35 kDa, respectively. The amino acid sequence analysis of the 47-kDa protein(EG47) showed a sequence similarity with fungal glycoside hydrolase family 5 endoglucanase from the white-rot fungus Phanerochaete chrysosporium. N-terminal and internal amino acid sequences of the 35-kDa protein(EG35), however, had no homology with any other glycosylhydrolases, although the enzyme had high specific activity against carboxymethyl cellulose, which is a typical substrate for endoglucanases. The initial rate of Avicel hydrolysis by EG35 was relatively fast for 48 h, and the amount of soluble reducing sugar released after 96 h was $100{\mu}g/ml$. Although EG47 also hydrolyzed Avicel, the hydrolysis rate was lower than that of EG35. Thin layer chromatography analysis of the hydrolysis products released from Avicel indicated that the main product was cellobiose, suggesting that the brown-rot fungus possesses processive EGs capable of degrading crystalline cellulose.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.215.3-215
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• 2005

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.93.1-93
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• 1999

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.228.1-228
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• 1999

No Abstract, See Full Text