• Biomedical Science Letters
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• v.18 no.1
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• pp.71-75
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• 2012

The PLA2G4A catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid, which is metabolized into lipid-based cellular hormones that regulate inflammatory response. The circulating blood cell numbers can be influenced by stress, infection or inflammation. Quantitative blood cell count traits analysis for the 19 SNPs in the PLA2G4A gene in the Korean Association Resource (KARE) cohort (7551 subjects) was performed. The only one SNP (rs10752979) in the all blood cell count was satisfied with the Bonferroni corrected P-value (<0.00263). Furthermore, 6 of the 19 SNPs in the PLA2G4A gene showed a weak or moderate association with blood cell count (P-values: 0.0048~0.042), suggesting the clue of an association between the PLA2G4A gene and blood cell count, especially white blood cell count. This study may provide insight into the genetic basis of blood cell count related with reaction of infection.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.1889-1891
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• 2004

The differential white blood cell count plays an important role in the diagnosis of different diseases. It is a tedious task to count these classes of cell manually. An automatic counter using computer vision helps to perform this medical test rapidly and accurately. Most commercial-available automatic white blood cell analysis composed mainly 3 steps including segmentation, feature extraction and classification. In this paper we concentrate on the first step in automatic white-blood-cell analysis by proposing a segmentation scheme that utilizes a benefit of active contour. Specifically, the binary image is obtained by thresolding of the input blood smear image. The initial shape of active is then placed roughly inside the white blood cell and allowed to grow to fit the shape of individual white blood cell. The white blood cell is then separated using the extracted contour. The force that drives the active contour is the combination of gradient vector flow force and balloon force. Our purposed technique can handle very promising to separate the remaining red blood cells.

• Clinical and Experimental Pediatrics
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• v.45 no.3
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• pp.325-330
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• 2002

Purpose : We intended to find out how the red cell transfusion would change the white cell count in preterm neonates under treatment in the intensive care unit. We also speculated whether the magnitude of such a change could indicate a potential neonatal infection. Methods : Total white blood cell count, total neutrophil count, and band count were compared and analyzed retrospectively on 33 preterm neonates who received red cell transfusions in our hospital's intensive care unit over a period of two years and a month. Results : We found a mean change of $1.33{\times}10^3/mm^3$ and $0.55{\times}10^3/mm^3$ in total white blood cell count and total neutrophil count in the first eight hours following the red cell transfusion. No significant change was observed in band count between pre and post-red cell transfusion. Conclusion : A mild increase in the white blood cell count caused by an increase in neutrophil count was observed temporarily following the red cell transfusion. But the white blood cell count returned to the pre-transfusion level in about 24 hours, indicating that such a low level of increase cannot be interpreted as an infection of a preterm neonate.

• Journal of Chest Surgery
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• v.2 no.1
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• pp.105-114
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• 1969

This experiment was carried out to study the responses of cellular component of blood and bone marrow to cold and also the changes of coagulation during cooling. Forty-two mongrel dogs were subjected to hypothermia by ice-water surface cooling technique. Lowest body temperature ranged from 21-23 degree. Dogs were divided into 3 groups,Group I, 12 dogs: pentothal anesthesia for 3 hours, Group II, 20 dogs;hypothermic group and Group III,10 dogs;postsplenectomy hypothermic group. Results were summarized as follows: 1. Hemoglobin, hematocrit and red blood cell count significantly increased when animals were cooled, and increase was noted in similar magnitude among the animals of Group I. 2. White blood cell count extremely decreased after cooling and effect of splenectomy on white blood cell count was not apparent. No significant changes were seen among Group I. 3. Differential count of white blood cell when cooled showed relative increase of polymorphonuclear neutrophil and decrease of lymphocyte. 4. There was marked decrease of platelets when body temperature reached to 21-23degree and essentially. no changes was noted in Group I. 5. Clotting time, bleeding time, plasma prothrombin time, recalcification time, and fibrinolysis showed no significant changes when dogs were cooled. Clot retration and prothrombin consumption during hypothermia appeared to be poor. In Group II, bleeding time decreased after splenctomy and when body temperature was lowered, plasma prothrombin time, clot retraction, and prothrombin consumption decreased. Decreased bleeding time and poor clot retraction were noted in Group I. 6. It was found that megacaryocyte count decreased even though platelet count of peripheral blood markedly diminsished when animals were cooled. There was some tendency of erythroid hyperplasia noted during hypothermia.

• Biomedical Science Letters
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• v.24 no.4
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• pp.334-340
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• 2018

The cytoplasmic elicitor, nucleotide-binding domain and leucine-rich repeat containing domain receptors (NLRs) is well established molecules in its role in inflammatory response. Among 22 NLR receptors, NOD2 is one of the intensively studied genes of elucidating for the inflammatory bowel disease and Crohn's disease as well. Recent research have accumulated that common genetic mutations in Parkinson's disease (PD) are increasingly related to the susceptibility to Crohn's disease. In this study, with the Korean Genome and Epidemiology Study, we aimed to perform the association between NOD2 polymorphisms and blood cell counts [WBC (white blood cell) count, RBC (red blood cell) count, platelet count], which linked supposedly to cytoplasmic inflammatory responses with clinical specialty. Linear regression analyses were performed, controlling for residential area, sex, and age as covariates. As a results, 12 SNPs from NOD2 gene were significantly associated with WBC counts (Bonferroni correction P-value criteria < 0.05/23=0.00218). In this study, we could ensure an association with NOD2 gene and WBC counts. This is the first report to have relationship between SNPs of NOD2 gene and WBC counts.

• Journal of Korean Physical Therapy Science
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• v.24 no.2
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• pp.1-8
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• 2017

Background: To determine the effect of Aerobic exercise(AE) on body temperature and blood components in smoking male subjects. Methods: 15 subjects were randomly assigned to Smoking group(smoking, n=8) and Non-smoking group(Non-smoking, n=7). To measure body temperature and blood components. For evaluation of body temperature, the Infrared Thermography, IT was used, and blood components was measured using the Complete Blood cell Count(CBC). Results: The results shows that White blood cell (WBC) was significantly (p<0.05) increased in Smoking group. and no significantly difference between groups(p<0.05). Regarding body temperature, was significantly (p<0.05) increased in Smoking group and Non-somking group. and no significantly difference between groups(p<0.05). Conclusion: Aerobic exercise can increase White blood cell and body temperature in smokers.

• Journal of Chest Surgery
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• v.38 no.10 s.255
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• pp.669-674
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• 2005

Background: There are several studies that report the relationship between leukocytosis and cardiovascular disease mortality. Most of these studies stand on the basis that atherosclerosis is mediated by inflammatory process. By the same token, preoperative white blood cell count is suggested as an independent predictable factor of postoperative mortality and morbidity in coronary artery bypass grafting. The purpose of this study is to define the influence of preoperative white blood cell count on postoperative morbidity and mortality after coronary artery bypass grafting. Material and Method: The medical records of the 133 patients who had undergone isolated coronary artery bypass grafting at Inha University Hospital from 1996 to 2003 were reviewed. Patients were evenly divided into four groups, and named as group A, B, C, and D respectively based on their preoperative white blood cell count in ascending order. The number of patients in each group were 33 with exception of 34 in group A. The range of white blood cell count were from $1.3\times10^3/{\mu}L\;to\;5.9\times10^3/{\mu}L\;in\;group\;A,\; from\;6.0\times10^3/{\mu}L\;to\;7.0\times10^3/{\mu}L\;in\;group\;B,\;from\;7.1\times10^3/{\mu}L\;to\;8.9\times10^3/{\mu}L$ in group C, and from $8.9\times10^3/{\mu}L\;to\;16.9\times10^3/{\mu}L$ in group D. Result: The number of patients with recent myocardial infarction was 0 in group A, $2(6.1\%)$ in group B, $4(12.1\%)$ in group C, and $8(24.3\%)$ in D group, showing proportional increase to the white blood cell count (p<0.01). There were six postoperative deaths; $1(2.9\%)$ in group A, $1(3.0\%)$ in group B, $2(2.6\%)$ in group C, and $2(6.1\%)$ in group D (p=0.44), showing no significant difference between the groups. Postoperative wound infection occurred in 3 patients; all 3 patients were in group D, showing that postoperative wound infection is closely related to the preoperative white blood cell count. Conclusion: The association between preoperative white blood cell count and postoperative mortality could not be defined. The incidence of postoperative wound infection was found to be proportional to the preoperative white blood cell counts.

• The Journal of Internal Korean Medicine
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• v.25 no.4
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• pp.117-128
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• 2004

Objectives : To examine the effects of Gojineumja on white rats which deteriorated immunity caused by Methotrexate(MTX), first of all, MTX was fed to the rats once a day for 4 day. Methods : After the immune response of the rats are deteriorated, dried extracts of Gojineumja(GJE) mixed in water was fed to the white rats once a day for l4days. The next conclusion was made by examining the rates of B-cells and T-cells of the peripheral blood and the changes in rates of CD4+ T-cells and CD8+ T -cells of the blood sampled from the spleen and peripheral region. Especially the count of CD3+ CD4+ T-cells of the peripheral blood and the count of CD3+ CD4+ T-cells of the spleen the count of CD4+/ CD8+ T-cell of the peripheral blood and the spleen proved the significant effect of increasing immune responses statistically. Results :(1) The following are the summary of the results. (2) The percentage of B lymphocyte of peripheral blood was increased significantly in GJE group as compared with control group. (3) The percentage of CD3+ CD4+ T-cell of peripheral blood was increased significantly in GJE group as compared with control group. (4) The percentage of CD3+ CD8+ T-cell of peripheral blood was not different statistically. (5) The percentage of CD4+/ CD8+ T-cell of peripheral blood was increased significantly in GJE group as compared with control group. (6) The percentage of CD3+ CD4+ T-cell of spleen was increased significantly in GJE group as compared with control group. (7) The percentage of CD3+ CD8+ T-cell was not different statistically. (8) The percentage of CD4+ /CD8+ T-cell was not different statistically. Conclusions : Gojineumja has an effect of increasing immune responses on white rats with deteriorated immunity caused by MTX.

• Journal of agricultural medicine and community health
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• v.35 no.1
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• pp.67-76
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• 2010

Objectives: This study was attempted to identify the relationship between white blood cell counts and the metabolic syndrome. Methods: This study included 394 adults who visited the medical checkup center placed in Gwangju, January 1, 2008 to December 31, 2008. Index of blood test and physical checkup were performed on the study such as triglyceride, HDL-cholesterol, fasting sugar and white blood cell counts. Logistic regression analysis was used to evaluate the association between white blood cell counts, white blood cell differential count and metabolic syndrome with an adjustment age and smoking status. Results: The prevalence rate of metabolic syndrome was 25.3% among males and 13.3% among females, and was particularly high among males in their 40s. The increase in white blood cell counts lead to high prevalence of metabolic syndrome for both males and females. As white blood cell counts increased, the values of body mass index and cardiovascular risk factors were increased significantly. The odds ratio for elevated white blood cell counts increased significantly in the subjects with each components of the metabolic syndrome compared to the subjects without them. The lymphocyte counts in the white blood cell differential counts were higher in patients with metabolic syndrome than in those without. Conclusions: High level of white blood cell counts in normal range can be used as indicator in chronic inflammation. Increased white blood cell counts were significantly associated with metabolic syndrome.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.20 no.2
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• pp.58-63
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• 2012

An altitude chamber, also known as a hypobaric chamber, is a device used during aerospace or high terrestrial altitude research or training to simulate the effects of high altitude on the human body. Although data from altitude chamber researches using experimental animals have been accumulated, studies in the humans exposed to hypobaric conditions are seldomly reported. Despite the importance of altitude chamber flight training in the field of aviation physiology, the hematological analysis of post-flight physiological changes has rarely been performed. The aims of the present study were to investigate the alterations in blood components during altitude chamber flight and to determine whether the differences between pre- and post-flight values are significant. Sixty experienced pilots in the Republic of Korea Air Force were enrolled in the altitude chamber flight training. Venous blood samples were obtained before and immediately after the flight. Compared with the pre-flight values($6.32{\times}10^3/mm^3$, $5.02{\times}10^6/mm^3$, 15.61 g/dL, respectively), white blood cell count, red blood cell count and hemoglobin level were significantly increased after the flight($6.77{\times}10^3/mm^3$, $5.44{\times}10^6/mm^3$, 16.26 g/dL; p=0.006, p=0.012, p<0.001, respectively). These alterations may be attributable to the exposure to hypobaric hypoxia, 100% oxygen supply for denitrogenation, considerable rise and fall in altitude and psychophysical stress due to these factors. In further studies, experimental groups and methods should be individualized to ensure objectivity and diversification. In addition, multiple time-frame analyses regarding the changing pattern of each blood component are also required to elucidate the physiological process for adapting to the high terrestrial altitude exposure.