• 한국조경학회지
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• 제44권2호
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• pp.96-107
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• 2016

본 연구는 창경궁 내 식물원이 조성되기 이전에 나타난 한국 근대 식물원의 효시를 찾고자 하는 시도이다. 이를 위해 1876년부터 1910년의 개항기에 일본, 러시아, 영국에 파견된 사절단이나 윤치호 및 유길준과 같은 해외 유학생이 작성한 기행문 형식의 글을 실마리 삼아 당시 한국 지식인들이 식물원을 이해하고 전유하는 양상을 읽어내려 하였다. 연구 결과, 식물원에 관한 내용은 1880년대까지만 하더라도 기행문에 등장하지 않았다. 여러 식물원을 안내하려고 했던 일본 측의 계획과 달리 일본에 파견된 조선 사절단들은 식물원을 방문하지 않고 관련 기록도 남기지 않는 등 식물원에 대한 흥미를 보이지 않았다. 반면, 1890년대 이후 러시아, 유럽, 미국 등지에 파견된 사절단이나 유학생들은 식물원에 관심을 보이기 시작하였다. 그들은 식물원을 문명국인 서구의 대표적 문물이자 필수품으로 판단하고 학문 발전과 계몽의 도구로서 국내에 도입하고자 노력하였다. 비록 개항기 한국 지식인들의 식물원의 필요성에 대한 인식 및 주장이 실제 식물원 조성으로 이어지지는 못하였으나, 이들의 기록과 견해는 문명의 표상으로서 식물원의 이미지를 공고히 하였다는 점에서 중요하다고 판단된다. 본 연구는 한국 근대 식물원 개념의 태동을 구체화하고 1909년 창경궁 내 식물원의 설립을 이해하는 데 밑거름을 제공하는 기초연구로서 의의를 갖는다.

• 한국산학기술학회논문지
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• 제13권11호
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• pp.5076-5082
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• 2012

본 연구는 교사의 권위가 약화되고, 교사와 학생의 관계가 각박해지고, 교사의 역할과 전문성 문제에서 논의가 엇갈리고 있는 우리 교육의 현 시점에서, 유가(儒家)전통의 교사상을 재 고찰해보는 것을 목적으로 하였다. 이를 위해 교사상을 권위상(權威像), 인애상(仁愛像), 사범상(師範像)으로 나누어 논의하였다. 유가의 전통에서 교사의 권위는 이성적 권위이며, 사회적 제도와 교사의 사회적 역할의 산물이고, 더 중요한 것은 권위보다는 화해를 강조하고 있었다. 유가전통에서는 인(仁)을 근본으로, 예(禮)를 그 방식과 수단으로, 교사와 학생간의 사랑과 정을 중시하고 있었다. 또 유가에서는 인간의 모범으로서, 교사의 양호한 도덕품성과 전문적 자질을 요구하고 있었다. 교사의 전문성과, 사회적 역할 그리고 인애(仁愛)의 정신이 교사의 권위를 형성하였고, 또 전체적인 유가 전통의 아름다운 교사상을 형성해왔던 것으로 보인다. 따라서 본고는 서양의 문화와 가치관의 영향을 많이 받고 있는 동양 사회의 현실에서, 우리의 교육현장에서 다시금 동양전통의 가치관과 그 의미를 재 사고해 볼 것을 권고하고 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권11호
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• pp.1644-1651
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• 2014

Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the foreign gene expression levels are also low because of their random integration in the host genome. In this study, we investigated the homologous recombination on the porcine ${\beta}$-casein gene locus using a knock-in vector for the ${\beta}$-casein gene locus. We developed the knock-in vector on the porcine ${\beta}$-casein gene locus and isolated knock-in fibroblast for nuclear transfer. The knock-in vector consisted of the neomycin resistance gene (neo) as a positive selectable marker gene, diphtheria toxin-A gene as negative selection marker, and 5' arm and 3' arm from the porcine ${\beta}$-casein gene. The secretion of enhanced green fluorescent protein (EGFP) was more easily detected in the cell culture media than it was by western blot analysis of cell extract of the HC11 mouse mammary epithelial cells transfected with EGFP knock-in vector. These results indicated that a knock-in system using ${\beta}$-casein gene induced high expression of transgene by the gene regulatory sequence of endogenous ${\beta}$-casein gene. These fibroblasts may be used to produce transgenic pigs for the production of therapeutic proteins via the mammary glands.

• 대한한방소아과학회지
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• 제29권4호
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• pp.39-66
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• 2015

Objectives The purpose of this study is to investigate the effects of Sesamum indicum extracted (SEI) on atopic dermatitis in an in-vitro and in-vivo experiment using a MC/9 murine mast cells and a NC/Nga mouse. Methods In-vitro experiment, IL-4, IL-5, IL-6, IL-13, TNF-${\alpha}$ and GM-CSF mRNA expression were evaluated by Real-time PCR, IL-13, MIP-$1{\alpha}$ production by ELISA and manifestations of NFAT-1, NFAT-2, c-jun, c-fos, NF-${\kappa}B$ p65 transcription factors by western blotting. In-vivo experiment, we measured WBC, Eosinophil, Neutrophil, and serum IL-5, IL-13 in NC/Nga atopic dermatitis mouse, IL-5, IL-13, IFN-${\gamma}$, IL-4 in the spleenocyte culture supernatant by ELISA, the absolute cell numbers of CD4+, CD8+, +Gr-1+CD11b, B220+CD23+ in the axillary lymph node (ALN), peripheral blood mononuclear cells (PBMCs) and dorsal skin tissue, IL-5, IL-13 by Real-time PCR, the distribution of tissue inflammation and cellular infiltration by H&E and toluidine blue. Results SEI decreased IL-4, IL-5, IL-6, IL-13, GM-CSF, TNF-${\alpha}$ mRNA expression, IL-13, MIP-$1{\alpha}$ production and the expression of transcription factors including NFAT-1, c-jun, NF-${\kappa}B$ p65 in MC/9 murine mast cells. SEI orally administration decreased cell number of WBC, Eosinophil, the level of serum IgE, total cell number of ALN and dorsal skin tissue, absolute cell number of CD4+, CD8+, B220+CD23+ in the ALN. SEI orally administration also increased absolute cell number of CD8+/CD3+ and decreased Gr-1+/CD11b+ in PBMCs, decreased CD4+ in dorsal skin tissue, inhibited IL-5, IL-13 mRNA expression. Infiltration levels of inflammatory immune cells, mast cells and thickness of epidermis decreased in dorsal skin tissue. Conclusions SEI can regulate allergic inflammatory response suppressed the gene expression and production of cytokines that mediate allergic reactions, and will be able to be effectively utilized in the treatment of atopic dermatitis future.

• IMMUNE NETWORK
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• 제3권1호
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• pp.69-77
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• 2003

Background: Celecoxib, a COX-2 specific inhibitor, has recently been used for the treatment of rheumatoid arthritis. However, the molecular and cellular mechanisms of celecoxib against RA inflammation remain to be defined. To elucidate the action mechanism of celecoxib on inflammatory cells, we investigated the effect of celecoxib on the production of two important mediators of inflammation, nitric oxide and PGE2 Methods: RAW 264.7 cells stimulated with LPS were preincubated with various concentrations of celecoxib (from $10^{-8}$ to $10^{-5}$ M) and $10{\mu}M$ hydrocortisone, respectively. The production of NO and PGE2, the end products of iNOS and COX-2 genes, were estimated in culture supernatants by Greiss method and EIA, respectively. The expression of iNOS gene, COX-2 gene, $NF-{\kappa}B$, and $I-{\kappa}B$ were determined by RT-PCR and western blot analysis. Results: Celecoxib and hydrocortisone inhibited the production of NO and PGE2 in dose dependent manner, when RAW 264.7 cells were stimulated with LPS. The expression of iNOS was also down-regulated by celecoxib and hydrocortisone. Interestingly, COX-2 gene differentially expressed according to the dose of celecoxib, a decrease with lower dose ($10^{-8}$ M) but an increase with higher dose ($10^{-5}$ M). $NF-{\kappa}B$ binding activity was decreased by lower dose of celecoxib, whereas was not affected by higher dose of it. The expression of $I-{\kappa}B$ was suppressed by higher dose of celecoxib. Conclusion: The celecoxib strongly suppressed the production of NO and PGE2 in LPS-stimulated RAW264.7 cells. The decrease of NO seems to be linked to the inhibition of iNOS by celecoxib. The lower and higher dose of celecoxib differentially regulated the COX-2 expression and $NF-{\kappa}B$ activity.

• 대한본초학회지
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• 제22권3호
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• pp.117-125
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• 2007

Objective : Lonicera japonica (Caprifoliaceae) has long been used for treatment of infectious diseases in oriental countries. The aim of this study was to investigative the effect by which the aqueous extract from flower of L. japonica (LJFAE) inhibited the lipopolysaccharide (LPS)-induced inflammatory mediators in murine macrophages, RAW 264.7 cells Methods : The dried flowers of L. japonica were extracted with distilled water at $100^{\circ}C$ for 7 h. The extract was filtered through 0.45 ${\mu}m$ filter, freeze-dried. The dried extract was dissolved in Hank's balanced salt solution (HBSS) and filtered through 0.22 ${\mu}m$ filter before use. Accumulated nitrite, an oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess reaction. The levels of prostaglandin E2 (PGE2), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-1$\beta$ (IL-1$\beta$), and IL-6 production, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were measured by enzyme-linked immunosorbent assay and Western blot analysis. Results: LJFAE (10-400 ${\mu}g$/ml) per se had no cytotoxic effect in unstimulated macrophages, but LJFAE concentration-dependently reduced NO, PGE2, TNF-, IL-l, and IL-6 production and COX-2 activity caused by stimulation of LPS. The levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with LJFAE in a concentration dependent manner. Conclusions : These results suggest that LJFAE suppress the NO and PGE2production in macrophages by inhibiting iNOS and COX-2 expression and these properties may contribute to the anti-inflammatory activity of Lonicera japonica.

• Journal of Periodontal and Implant Science
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• 제40권3호
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• pp.105-110
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• 2010

Purpose: It has been reported that low-level semiconductor diode lasers could enhance the wound healing process. The periodontal ligament is crucial for maintaining the tooth and surrounding tissues in periodontal wound healing. While low-level semiconductor diode lasers have been used in low-level laser therapy, there have been few reports on their effects on periodontal ligament fibroblasts (PDLFs). We performed this study to investigate the biological effects of semiconductor diode lasers on human PDLFs. Methods: Human PDLFs were cultured and irradiated with a gallium-aluminum-arsenate (GaAlAs) semiconductor diode laser of which the wavelength was 810 nm. The power output was fixed at 500 mW in the continuous wave mode with various energy fluencies, which were 1.97, 3.94, and 5.91 $J/cm^2$. A culture of PDLFs without laser irradiation was regarded as a control. Then, cells were additionally incubated in 72 hours for MTS assay and an alkaline phosphatase (ALPase) activity test. At 48 hours post-laser irradiation, western blot analysis was performed to determine extracellular signal-regulated kinase (ERK) activity. ANOVA was used to assess the significance level of the differences among groups (P<0.05). Results: At all energy fluencies of laser irradiation, PDLFs proliferation gradually increased for 72 hours without any significant differences compared with the control over the entire period taken together. However, an increment of cell proliferation significantly greater than in the control occurred between 24 and 48 hours at laser irradiation settings of 1.97 and 3.94 $J/cm^2$ (P<0.05). The highest ALPase activity was found at 48 and 72 hours post-laser irradiation with 3.94 $J/cm^2$ energy fluency (P<0.05). The phosphorylated ERK level was more prominent at 3.94 $J/cm^2$ energy fluency than in the control. Conclusions: The present study demonstrated that the GaAlAs semiconductor diode laser promoted proliferation and differentiation of human PDLFs.

• Biomolecules & Therapeutics
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• 제23권2호
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• pp.189-194
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• 2015

P450 1A2 is responsible for the metabolism of clinically important drugs and the metabolic activation of environmental chemicals. Genetic variations of P450 1A2 can influence its ability to perform these functions, and thus, this study aimed to characterize the functional significance of three P450 1A2 allelic variants containing nonsynonymous single nucleotide polymorphisms (P450 $1A2^*8$, R456H; $^*15$, P42R; $^*16$, R377Q). Variants containing these SNPs were constructed and the recombinant enzymes were expressed and purified in Escherichia coli. Only the P42R variant displayed the typical CO-binding spectrum indicating a P450 holoenzyme with an expression level of ~ 170 nmol per liter culture, but no P450 spectra were observed for the two other variants. Western blot analysis revealed that the level of expression for the P42R variant was lower than that of the wild type, however the expression of variants R456H and R377Q was not detected. Enzyme kinetic analyses indicated that the P42R mutation in P450 1A2 resulted in significant changes in catalytic activities. The P42R variant displayed an increased catalytic turnover numbers ($k_{cat}$) in both of methoxyresorufin O-demethylation and phenacetin O-deethylation. In the case of phenacetin O-deethylation analysis, the overall catalytic efficiency ($k_{cat}/K_m$) increased up to 2.5 fold with a slight increase of its $K_m$ value. This study indicated that the substitution P42R in the N-terminal proline-rich region of P450 contributed to the improvement of catalytic activity albeit the reduction of P450 structural stability or the decrease of substrate affinity. Characterization of these polymorphisms should be carefully examined in terms of the metabolism of many clinical drugs and environmental chemicals.

• 한국언론정보학보
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• 제28권
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• pp.111-140
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• 2005

일제는 중일전쟁 발발 이후 내선일체론에 근거한 황민화 정책을 실시하고, 병참기지론에 기반하는 전쟁동원체제의 구축을 서둘렀다. 중일전쟁을 위해서는 조선인을 동원할 필요가 있었고, 그러기 위해서는 조선인의 황민화가 이루어져야만 했기 때문이다. 또한 일제는 황민화와 전쟁동원을 위해 전쟁의 성격과 목적을 정당화하는 시도를 해야만 했다. 본 연구는 이런 일제의 지배정책을 다룬 $\ll$동아일보$\gg$와 $\ll$조선일보$\gg$의 사설을 분석한 것이다. 먼저 전쟁의 성격과 목적에 관한 사설에서 두 신문은 중일전쟁 발발직후 한 동안 일본은 부득이하게 동양의 평화를 위해 전쟁에 나섰다고 주장했고, 일본군의 승전 소식이 있을 때마다 이를 대대적으로 선전했다. 1938년 말 이후 두 신문은 중일전쟁은 동양을 서구로부터 해방시키기 위한 전쟁이고, 일본은 그 해방자라고 주장했다. 다음으로 황민화 정책과 관련해 두 신문은 초기에는 주로 신사참배나 궁성요배를 정당화하고 권유하는 논조를 보였을 뿐이다. 이후 두 신문 모두 지원병제도에 대해서는 '완전한 국민'이 될 수 있는 기회를 얻었다고 절대적인 찬성 입장을 보였던 반면에 3차 조선교육령에 대해서는 조선어 교육의 폐지를 염려하며 소극적이나마 반대의사를 표명했다. 1939년에 들어서서 두 신문 모두 '일본 정신'을 강조하며 내선일체를 적극적으로 주장하기 시작했다. 마지막으로 전쟁동원 정책에 관한 사설에서 두 신문은 전쟁 직후 한 동안 자중을 요구하는 논조만을 보이다가, 곧 여기에서 벗어나 적극적으로 전쟁협력을 촉구하는 논조를 보이기 시작했다. 1939년에 들어서서 '일본 정신'을 기반으로 하여 적극적으로 전쟁에 협력해야 한다는 주장들이 사설에서 나타났고, 1940년에 가서는 이제 국가주의적이고 전체주의적인 동원체제를 강력히 주장하기에 이르렀다. 이 같은 논조는 일제의 강력한 언론통제와 신문사 내 경영진 및 언론인의 의식전환이 모두 작용한 결과였고, 결국 두 신문이 일제의 지배방식에 순응해 독자들에게 내선일체와 전쟁협력을 강요했다는 역사적 평가를 듣게 만들었다.

• Journal of Nutrition and Health
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• 제36권9호
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• pp.908-917
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• 2003

It has been proposed that oxidative modification of LDL (oxLDL) plays a significant role in the pathogenicity of atherogenesis. We tested the hypothesis that chitin and chitosan may function as antioxidants with respect to 0.1 mg cholesterol/ml LDL incubated with 5 $\mu$ M Cu$^2$$^{+}$alone or in the P338Dl mouse macrophage system using L-ascorbic acid as a standard classical antioxidant. The degree of oxLDL formation was ascertained by the relative electrophoretic mobility (rEM) in the combination of thiobarbituric acid reactive substances (TBARS) levels, and the cytotoxicity of oxLDL was detected by macrophage viability. The oxLDL uptake and foam cell formation of macrophages were measured by Oil Red O staining. Incubation with Cu$^2$$^{+}$and macrophages increased rEM of LDL and stimulated TBARS formation. Culture of macrophages with LDL in the presence 5 $\mu$ M Cu$^2$$^{+}$induced macrophage death. In cell-free system 200 $\mu$g/ml water-soluble chitosan and chitosan-oligosaccharide blocked oxLDL formation. Water-soluble chitosan and chitosan-oligosaccharide blocked oxLDL formation near-completely relative to L-ascorbic acid, whereas water-soluble chitin and chitin-oligosaccharide had no measurable antioxidant effect. In macrophage system water-soluble chitosan and chitosan-oligosaccharide blocked oxidation of LDL with a significant increase in cell viability, and decreased TBARS in medium. As for the inhibitory effect on macrophage foam cell formation, chitosan and its oligosaccharide, but not watersoluble chitin, revealed the effectiveness. The endothelial expression of lectin-like oxLDL receptor-1 (LOX-1) was tested by Western blot analysis, and chitosan, chitosan-oligosaccharide and chitin-oligosaccharide blocked LOX-1 expression. These results indicate that water-soluble chitosan and its oligosaccharide showed the inhibitory effect on Cu$^2$$^{+}$-induced LDL oxidation of macrophages, and chitosan, chitosan-oligosaccharide and chitin-oligosaccharide had blocking effect on oxLDL receptor expression in the human umbilical vein endothelial system. Thus, water-soluble chitosan and its oligosaccharides possess anti-atherogenic potentials possibly through the inhibition of macrophage LDL oxidation or endothelial oxLDL receptor expression depending on chemical types.l types.