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Diagnosis, Pathology, and Taxonomy of Perkinsus sp. Isolated from the Manila clam Ruditapes philippinarum in Korea

  • Choi, Kwang-Sik;Park, Kyung-Il;Cho, Moon-Jae;Soudant, Philippe
    • Journal of Aquaculture
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    • v.18 no.3
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    • pp.207-214
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    • 2005
  • We report on the diagnosis, pathology, and taxonomy of Perkinsus sp. infection in Manila clams (Ruditapes philippinarum) from Korean waters. Amplimers were designed from internal portions of the non-transcribed spacer (NTS) of P. atlanticus for molecular diagnosis of Perkinsus infection. PCR-based identification methods and an in situ hybridization assay were developed for detection of Perkinsus sp. in live tissues as well as in histological preparations. Hybridization signals were observed around the nucleus of trophozoites. Positive results from PCR and in situ hybridization indicated that Korean Perkinsus sp. is genetically identical with P. atlanticus reported in Europe, which is currently synonymous with P. olseni reported from Australia. Microscopic morphological features of different lift stages of Perkinsus sp. appeared very similar to those of P. atlanticus. Severely infected clams often exhibited white nodules on their mantles and gills as a consequence of inflammation. In lightly to moderately infected clams, Perkinsus sp. was mainly found in gill tissues, whereas the protozoan parasites were found in digestive tracts, gonadal tissues, and foot tissues of heavily infected clams. It is likely that the gills are the portal of the infection and that P. olseni spreads to other tissues as the infection advances. In conclusion, by considering the taxonomic priority of P. olseni, Korean Perkinsus sp. is accepted as P. olseni. P. olseni appears to be common on tidal flats on the western and southern Korean coasts and is considered to be a pathogen capable of causing mass mortality of clams.

A Study on the Evaluation of Berthing Energy of Large-Sized Container Ships with the effect of Shallow Waters (대형 컨테이너선의 천수역 영향을 고려한 접안에너지 산출에 관한 연구)

  • Kim Chol-Seong;Lee Yun-Sok;Lee Chung-Ro;Cho Ik-Soon
    • Journal of Navigation and Port Research
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    • v.29 no.8 s.104
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    • pp.673-678
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    • 2005
  • In order to improve the safety of ship berthing and the efficiency of berth operation in the harbour, the berthing energy acting on a ship in berthing maneuver need to be estimated properly. The berthing energy is used as one of the criteria to determine the maximum permissible load q{ fender as well as important factors to establish the berthing speed and the required power of tug-boat for pilot and ship operator. Some problems of berthing energy are discussed on the basis of the hydrodynamic aspects. Then, series calculations of berthing energy are carried out considering the effect of water depth on added mass and the ship shape for container series from 1,600TEU to 12,000TEU.

The Identification of Human Unsafe Acts in Maritime Accidents with Grey Relational Analysis

  • Liu, Zhengjiang;Wu, Zhaolin
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2004.08a
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    • pp.139-145
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    • 2004
  • It is well known that human errors is involved in most of maritime accidents. For the purpose of reducing the influence of human elements on maritime activities, it is necessary to identify the human unsafe acts in those activities. The commonly used methods in identification of human unsafe acts are maritime accident statistics or case analysis. With the statistics data, people could roughly identify what kinds of unsafe acts or human errors have played active role in the accident, however, they often neglected some active unsafe acts while overestimated some mini-unsafe acts because of the inherent shortcoming of the methods. There should be some more accurate approaches for human error identification in maritime accidents. In this paper, the application of technique called grey relational analysis (GRA) into the identification of human unsafe acts is presented. GRA is used to examine the extent of connections between two digits by applying the, methodology of departing and scattering measurement to actual distance measurement. Based on the statistics data of maritime accidents occurred in Chinese waters in last 10years, the relationship between the happening times of maritime accidents and that of unsafe acts are established with GRA. In accordance with the value of grey relational grade, the identified main human unsafe acts involved in maritime accidents are ranked in following orders: improper lookout, improper use of radar and equivalent equipment, error of judgment, act not in time, improper communication, improper shiphandling, use of unsafe speed, violating the rule and ignorance of good seamanship. The result shows that GRA is an effective and practical technique in improving the accuracy of human unsafe acts identification.

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Algal Pigments and their Degradation Products in Suspended and Sinking Particulate Material in the Gulf of Mexico (멕시코 만의 부유 및 침강고형물에 존재하는 조류색소 및 그 분해산물)

  • Noh, Il
    • Journal of Environmental Science International
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    • v.5 no.2
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    • pp.153-170
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    • 1996
  • Photosynthetic pigments and their degradation products in suspended and sinking particles collected from the Gulf of Mexico waters 1987~88, were measured using High Performance liquid Chromatography (HPIC). The short term variations in flux rates of chlorophylls and carotenoids as well as-their degradation products were compared at the mesoscale cyclonic and anticyclonic circulation features (cold joie rink and warm core ring). Chlorophyll a was the predominant porphyrin of suspended particulate matter at both CCR -and WCR. Among carotenoid pigments, 19'-hexanoyloxyfucoxanthin, which is a biomarker of prymnesiophytes, was dominant pigment at both rinds. Phaeophorbide a, which is produced through the Brazing processes of grazers, was the predominant degraded pigment in sinking particles ai the study aiea. Total pigment flux in CCR was an order of magnitude higher than that in WCR. Less than l% of the standing stock of the pigments measured sank out of the upper 200m of the WCR on any given day. Thus, suspended particulate matter in Gulf of Medico was not recycled rapidly.

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The Massive Blooms of Gonyaulax polygramma (Gonyaulacales, Dinophyceae) in the Southern Coastal Areas of Korea in Summer, 2009 (2009년 남해안 전역에 발생된 고니아룩스 적조에 관한 연구)

  • Cho, Eun-Seob
    • Journal of Environmental Science International
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    • v.20 no.12
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    • pp.1521-1531
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    • 2011
  • This study was carried out to determine the characteristics of the marine environment, including nutritional content, in order to clearly understand the outbreaks of Gonyaulax polygramma in the southern coastal areas in August, 2009. Samples were collected at 13 sites and water temperature and salinity were measured using a CTD. Field surveys were twice: the first between August 5-7, the second between August 22-24. The cell density of G. polygramma was 6,500-10,000 cells $ml^{-1}$ during the $1^{st}$ survey, whereas during the $2^{nd}$ survey the range of the cells was recorded from 8,000 to 12,500 cells $ml^{-1}$. Cochlodinium polykrikoides ranged from 0 to 105 cells $l^{-1}$ during the field survey. In water environments, the majority stations during the $1^{st}$ survey showed a nearly homogeneous water column below $1^{\circ}C$ in temperature, as well as similar profiles of salinity. However, the stratification between the surface and bottom was observed in the $2^{nd}$ survey. Regarding nutrients, Dissolved Inorganic Nitrogen (DIN) in the surface ranged from 0.144 to 0.236 mg $l^{-1}$ during the $1^{st}$ survey, and 0.082-0.228 mg $l^{-1}$ during the $2^{nd}$ survey. DIP (Dissolved Inorganic Phosphorus) did not show any difference in concentration between the $1^{st}$ and $2^{nd}$ survey. During August of 2009, the wind speed in the southern waters remained at around ${\leq}2\;m\;s^{-1}$ for about 60% of time, and there was very little precipitation during the month. Irradiance of ${\geq}10$ hr was shown in the late of August. It is thought that a low level of DIN and salinity play an important role as an essential factor for rapid growth, wide distribution and longer duration of red tide in G. polygramma.

Reorganization of the International Fisheries Regime in the East Sea - Case by Korea and Japan Squid Angling Fisheries - (동해에 있어서 한국과 일본 오징어채낚기어업의 재편방향)

  • 김대영
    • The Journal of Fisheries Business Administration
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    • v.32 no.2
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    • pp.51-72
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    • 2001
  • This study intends to review the development of Squid Angling Fisheries of Korea and Japan and subsequent changes in the fisheries regime as well as management conditions and to examine reorganization directions. Although catches and productivity are all increasing in Korea and Japan as the squid resource in the East Sea is improving, the number of fishing vessels and the conditions of fisheries management are showing a symmetrical relationship. The former is increasing in Korea and decreasing in Japan while the latter is aggravating in Korea and rather stable in Japan. And while the competition between the two countries for more fishing grounds and catches are not that serious since the resource is relatively increasing, the catch and price competition with other domestic fisheries is a more significant problem. Furthermore, even though light restriction is implemented in both countries in order to suppress over-competition, it is adopted as a means to curtail management costs and block over-investment rather than to adjust international or inter-fisheries circumstances. Japan has been implementing the TAC system on squid resources since 1998. However, it has a different meaning than as a resource management method in its original sense because it is based on the highest catch level of the past and takes into consideration the fishermen's management conditions. In case of Korea, it is necessary to establish a plan to strengthen its fisheries management competence to address its shrinking catch in the Japanese waters. The developments mentioned so far have appeared amidst the increase of squid resources, and if such conditions disappear, problems such as international allocation of catches and fishing seasons, access to fishing grounds, and domestic conflicts among different fisheries will emerge.

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GROWTH OF TRANSPLANTED PORTUGAL AND OLYMPIA OYSTERS IN THE KOREAN COASTAL WATERS (Portugal굴(Crassostrea angulata) 및 Olympia굴(Ostrea Iurida)의 이식 성장에 관한 연구)

  • BAE Gyung-Man;BAE Pyung-Arm
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.5 no.1
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    • pp.17-22
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    • 1972
  • This results concerning the growth of transplanting of the oyster seed, Crassostrea angulata and Ostrea Iurida, which were transplanted from Japan by air, and cultivated by the raft suspended method at the coast of Yeongoo-Ri, Koje-Goon, located on the southern coast of Korea from January to December 1970, are as follows, 1. The mean shell height of O. lurida was 36.4mm, and the shape of the shell was round and small. On the other hand, the mean shell height of C. angulata was 87.4mm and the shell length was 52.3mm, and the shape of the shell was oval and large, 2. O. lurida and C. angulata oysters grew well when the water temperature was above $16^{\circ}C$ and the specific gravity ranged from 1.0171 to 1.0236 from June to July. 3. There was a little difference in relative valiance between shell length and height of O, lurida but significant difference was shown in C. angulata from July to November. 4. The mortality rate of O. lurida was $35.2\%$ and that of C. angulata was $21.7\%$ respectively.

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On the Trophic Correlation between Tintinnids and Dinoflagellates in Masan Bay, Korea (마산만의 유종섬모충류와 와편모류간의 포식-피포식 상관관계에 대하여)

  • YOO Kwang-Il;LEE Joon-Baek
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.3
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    • pp.230-236
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    • 1987
  • The correlation between tintinnid and dinoflagellate by means of seasonal variation of standing crops was investigated at two selected stations in Masan Bay, well-known as a red tide zone in southern coastal waters of Korea, during the period from January 1981 to December 1982. The most dominant dinoflagellates mainly belonged to $20-60\;{\mu}m$ of size class, and Gymnodinium and Prorocentrum were predominant from spring to summer season. Of tintinnid, Favella spp. were most dominant and associated with dinoflagellate blooms at the same season. Especially, Favella spp. were most positively correlated with $40-60\;{\mu}m$ size class of dinoflagellate, and also represented higher multiple corrleation with outer station (St.2), comprising relatively large species of Gymonodinium and Protogonyaulax, than with inner station (St. 1), dominated by Prorocentrum blooms. Thus, the interspecific food selection by size and morphology between tintinnid including Favella and dinoflagellate is recognized and it is considered to be an important factor influenecing on the prey-predator relationship in lower trophic level in the surveyed area.

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Changes in Fish Viral Disease Outbreaks in the Coastal Area of Korea Due to Increasing Water Temperature, an Impact of Climate Change (기후변화에 기인한 연안 수온상승에 따른 연안 어류의 바이러스성 질병 발생 예측)

  • Kim, Wi-Sik;Kim, Seok-Ryel;Park, Myoung-Ae;Lee, Joon-Soo;Avunje, Satheesha;Kim, Do-Hyung;Oh, Myung-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.46 no.5
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    • pp.582-588
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    • 2013
  • The impact of global warming on aquatic systems has been a priority research area in the past decade. However, the possibility that increased temperatures will cause shifts in viral disease outbreaks has not been well addressed. In the present study, with increasing water temperature (WT) in the coastal area of Korea, we estimated the possibility of changes in fish viral diseases. From the present time, WT may rise between 0.62 and $1.7^{\circ}C$ by 2050, and the effect on aquaculture could be more adverse than benefitial. Red seabream iridovirus disease (RSIVD) and viral nervous necrosis (VNN) cause high mortality above 22 and $24^{\circ}C$, respectively, and outbreaks could commence earlier and persist for prolonged periods. Nevertheless, the period of occurrence of viral hemorrhagic septicemia (VHS), which outbreaks at a lower WT (< $18^{\circ}C$), could be shorter than the current infectious period. Thermal stress in fish causes reductions in growth and immunocompetence; thus, increases in summer WT can lead to the development of new viral diseases. WT has a strong influence on fish population dynamics; therefore, entry of new viruses and changes in the prevalence of infection can be expected if carrier fishes are introduced or migrate to Korean waters.

Biotechnological Potential of Korean Marine Microalgal Strains and Its Future Prospectives

  • Hong, Ji Won;Kang, Nam Seon;Jang, Hyeong Seok;Kim, Hyung June;An, Yong Rock;Yoon, Moongeun;Kim, Hyung Seop
    • Ocean and Polar Research
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    • v.41 no.4
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    • pp.289-309
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    • 2019
  • Marine microalgae have long been used as food additives and feeds for juvenile fish and invertebrates as their nutritional content is beneficial for humans and marine aquaculture species. Recently, they have also been recognized as a promising source for cosmeceutical, nutraceutical, and pharmaceutical products as well as biofuels. Marine microalgae of various species are rich in multiple anti-oxidant phytochemicals and their bioactive components have been employed in cosmetics and dietary supplements. Oil contents in certain groups of marine microalgae are extraordinarily rich and abundant and therefore have been commercialized as omega-3 and omega-6 fatty acid supplements and mass production of microalgae-based biodiesels has been demonstrated by diverse research groups. Numerous natural products from marine microalgae with significant biological activities are reported yearly and this is attributed to their unique adaptive abilities to the great diversity of marine habitats and harsh conditions of marine environments. Previously unknown toxin compounds from red tide-forming dinoflagellates have also been identified which opens up potential applications in the blue biotechnology sector. This review paper provides a brief overview of the biotechnological potentials of Korean marine microalgae. We hope that this review will provide guidance for future marine biotechnology R&D strategies and the various marine microalgae-based industries in Korea.