• Title/Summary/Keyword: Water soluble fraction

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Physiological Effects and Sensory Characteristics of Mulberry Fruit Wine with Chongilppong (청일뽕 오디를 이용하여 제조된 침출주의 관능평가 및 생리활성연구)

  • 김현복;이용우;이완주;문재유
    • Journal of Sericultural and Entomological Science
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    • v.43 no.1
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    • pp.16-20
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    • 2001
  • The study was carried out to investigate sensory characteristics of Chongilppong mulberry fruit wine, as well as biological effects of subfraction of MeOH extracts. In the cholesterol-induced hyperlipidemia rats administered with subfraction of mulberry fruit extract, serum triglyceride, GPT and GOT level in serum were generally decreased. Therefore, the above results suggested that mulberry fruit water soluble fraction can help to maintain normal liver functions and to protect hyperlipidemia.

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Spinacine from panax ginseng

  • Han, Young-Nam;Ryu, Si-Yong;Han, Byung-Hoon;Woo, Lin-Keun
    • Archives of Pharmacal Research
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    • v.10 no.4
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    • pp.258-259
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    • 1987
  • An alkaloid was isolated form water-soluble fraction of Panax ginseng roots. It was characterized by spectroscopic data and dynthesis as 4, 5, 6, 7-tetrahydroimidazo (4, 5-c) pyridine-6-carboxylic acid or spinacine, which was first isolated from the plant kingdom.

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Phenolic Compounds from Barks of Pinus densiflora siebold et Zuccarini and Their NO Production Inhibitory Activities.

  • Kwon, Young-Min;Yeom, Seung-Hwan;Kim, Min-Ki;Lee, Jae-Hee;Lee, Min-Won
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.374.1-374.1
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    • 2002
  • The barks o.f Pinus densiflara representative Pinus species graws in Korea, have been used for oriental traditional medicine as the remedies for rheumatitis. hemorrhage and cancer. Water soluble fraction of 80% acetone extracts from the barks af Pinus densiflera (PDB) showed nitrogen monoxide(NO) production inhibitory activity in IFN-${\gamma}$. LPS stimulated RAW 264.7 cell. (omitted)

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Effect of Azadirachta indica flower extract on basal and experimentally elevated blood glucose in rats

  • Waliullah, S;Javed, Kalim;Jafri, MA;Singh, S
    • Advances in Traditional Medicine
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    • v.8 no.3
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    • pp.302-310
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    • 2008
  • Azadirachta indica A. Juss (N.O. Meliaceae), popularly known, as 'Neem' is an indigenous tree widely available in India. Almost every part of the tree has long been used in Unani system of medicine for the treatment of a variety of human ailments. The flowers have been mentioned as a remedy useful in controlling diabetes mellitus. The present study had been designed to investigate the hypoglycemic/anti-hyperglycemic effects of the methanolic extract of the flowers of A. indica (Gule-Neem) and its different fractions on normal, glucose fed hyperglycemic, adrenaline induced hyperglycemic and alloxan induced diabetic rats. The methanolic extract was resolved into water soluble and water insoluble fractions. Water soluble portion of the methanolic extract was found to possess significant blood sugar lowering effect in glucose-fed and adrenaline-induced hyperglycemic rats but it did not show such effect in normal and alloxan induced mild and severe diabetic rats. Water-soluble portion was fractionated by employing the polarity criterion with ethyl acetate and butanol. The ethyl acetate fraction was further fractionated into phenolic and non-phenolic fractions. Hypoglycemic effect of these fractions was also evaluated. The results suggest that the flowers of A. indica contain at least two different constituents, responsible for the said activity. These investigations validate the use of flowers of A. indica in diabetes by Unani physicians.

Thermal and Hygroscopic Properties of Indoor Particulate Matter Collected on an Underground Subway Platform

  • Ma, Chang-Jin;Lee, Kyoung-Bin;Zhang, Daizhou;Yamamoto, Mariko;Kim, Shin-Do
    • Asian Journal of Atmospheric Environment
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    • v.9 no.3
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    • pp.228-235
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    • 2015
  • In order to clarify the thermal and hygroscopic properties of indoor particulate matter (PM) in a semiclosed subway space, which is critically important for understanding of the distinctive particle formation processes as well as the assessment of their health effects, the size-resolved PMs (i.e., $PM_{2.5}$ and $PM_{10-2.5}$) were intensively collected on the platform of Miasageori station on the Seoul Subway Line-4. The elemental concentrations in soluble and insoluble fractions were determined by PIXE from the bulkily pretreated $PM_{2.5}$. The thermal and hygroscopic characteristics of individual particles were investigated via a combination of the unique pretreatment techniques (i.e., the high-temperature rapid thermal process and the water dialysis) and SEM-EDX analysis. Iron and calcium were unequaled in insoluble and soluble $PM_{2.5}$ fractions, respectively, with overwhelming concentration. The SEM-EDX's elemental net-counts for the pre- and post-pyrolyzed PMs newly suggest that magnesium and several elements (i.e., silica, aluminum, and calcium) may be readily involved in the newly generated subway fine PM by a high-temperature thermal processing when trains are breaking and starting. Through the water dialysis technique, it turned out that calcium has meaningful amount of water soluble fraction. Furthermore, the concentrations of the counter-ions associated with the calcium in subway $PM_{10-2.5}$ were theoretically estimated.

Removal Characteristics of Arsenic from Abandoned Metal Mining Tailings by Electrokinetic Technique (동전기법에 의한 폐 중금속광산 퇴적토 내의 비소제거 특성)

  • Shin Hyun-Moo;Yoon Sam-Seok
    • Journal of Environmental Science International
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    • v.15 no.3
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    • pp.279-286
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    • 2006
  • Electrokinetic technique was considered in removing arsenic from the abandoned mining tails. In order to estimate the removal characteristics of arsenic, the sequential extraction analysis and desorption experiment were carried out prior to the application of electrokientic process. The result of sequential extraction analysis indicated that the water soluble and exchangeable fraction, easily leachable to ground water, were very low as much as about 2.5% and the fraction except residual (38.3%), possibly extractable under very acidic or alkalic environment, was about 59%. In the result of desorption test using four different kinds of electrolytes, the mixture of citric acid and sodium dodecyl sulfate (SDS) showed the highest desorption efficiency as much as 77.3%. The removal efficiencies of arsenic from mining tailings by electrokinetic process under the different electrolyte environments were slightly low and resulted in the following order: citric acid + SDS (18.6%) > 0.1 $NHNO_3$ (8.1%) > HAc (7.4%) > Distilled water(6.6%). Also, arsenic in soil matrix was moved favorably in the direction of anodic rather than cathodic region, which is opposite trend with cationic metal ions generally existing in soil, because anionic form of arsenic is dominated in acidic soil caused by the movement of acid front form anode.

Inhibitory Activity of the Fruit Extract of Gardenia jasminoides on Monoamine Oxidase (치자추출물의 Monoamine Oxidase 저해활성)

  • Park, Tae-Kyu;Hwang, Keum-Hee
    • Korean Journal of Pharmacognosy
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    • v.38 no.2 s.149
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    • pp.108-112
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    • 2007
  • We examined the inhibitory activities against monoamine oxidase (MAO) of Gardenia jasminoides in vitro and in vivo methods. Methanolic extract and ethylacetate fraction of Gardenia jasminoides fruit showed a significant inhibitory activity on MAO-A and MAO-B in vitro. The IC$_{50}$ values of each fraction on MAO-A and MAO-B are as fo11owed; total methanol extracts 1.23 and 1.34 mg/ml, EtOAc fraction 0.72 and 0.77 mg/ml. Water-soluble fraction also showed IC$_{50}$ values of 0.81 mg/ml on MAO-B. MAO-A activity was increased by the oral administration of ethanolic extract of G. jasminoides, while MAO-B activity was decreased. The concentration of serotonin of brain tissue administrated of ethanolic extract of G. jasminoides is slightly increased in rat. This tendency is not different from the activity of deprenyl which is a well known MAO inhibitor was used as a positive control. Consequently, we suggest that G. jasminoides may have the effects on the inhibitory activity against MAO This activity of G. jasminoides is considerable for development of functional materials for treatment and control of depression, dementia, Parkinson' disease, stress and promoting exercise, etc.

The Nitro-scavenging Effects by the Component of Cassiae Torae Semen (결명자의 아질산염 소거작용)

  • Do, Jeong-Ryong;Kim, Seon-Bong;Park, Yeung-Ho;Park, Yeung-Beom;Choi, Jae-Sue;Kim, Dong-Soo
    • Korean Journal of Food Science and Technology
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    • v.25 no.5
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    • pp.526-529
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    • 1993
  • The present study was conducted to investigate nitrite-scavenging ability of the components of Cassiae torae semen, Cassia tora L. The nitrite-scavenging effects of fractions obtained from methanol extract of Cassiae torae semen, Cassia tora L. were strong in ethyl acetate fraction, chloroform fraction, water soluble fraction and ethyl ether fraction in order. Compound D showed the strongest nitrite-scavenging effects among compound A, B, C, D separated from Cassiae torae semen. Compound D separated from Cassiae torae semen possessed ten-fold stronger the nitrite-scavenging effect than ascorbic acid.

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Apigenin Derivatives of Paulownia coreana Uyeki Leaves

  • Si, Chuan-Ling;Kim, Jin-Kyu;Kwon, Dong-Joo;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.2
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    • pp.83-87
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    • 2006
  • The leaves of Paulownia coreana Uyeki were extracted with acetone-$H_2O$ (7:3, v/v), concentrated under reduced pressure and fractionated successively with n-hexane, methylene chloride and ethyl acetate, leaving residual water soluble fraction. A portion of the resulting aqueous soluble powder was chromatographed on a Sephadex LH-20 column using aqueous methanol and ethanol-hexane as washing solvents. Three apigenin derivatives were isolated and identified as apigenin-7-O-${\beta}$-D-glucpyranoside, apigenin-7-O-${\beta}$-D-glucuronopyranoside and apigenin-7-O-[${\beta}$-D-glucuronopyranosyl($1{\rightarrow}2$)-O-${\beta}$-D-glucuronopyranoside] by spectroscopic methods including NMR and FAB-MS.

Purification and Characterization of Crystalins by Aqueous Two-Phase Extraction

  • Bermudez, Ondrea;Forciniti, Daniel
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.6
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    • pp.395-401
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    • 2001
  • Crystallins are a family of water-soluble proteins that constitute up to 90% of the wa-ter-soluble proteins in mammalian eye lenses, We present in this paper an alternative purification method for these proteins using polyethylene glycol/dextran aqueous two-phase extraction. Un-der the appropriate conditions, we were able to recover the γ-crystallin fraction essentially free of the remaining proteins. High concentrations of salt at a neutral pH maximize the recovery of γ-crystallins in the top phase and minimize the contamination by the other proteins present in the lenses. The proposed protocol decreases the separation time by about 50%. The complex partition behavior observed for these proteins reflects a delicate balance between protein/phase-forming species(various polymers and salts) and protein interactions. This is evidenced, in part, by the role played by the largest proteins in this group as a "pseudo"phase-forming species.

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