• Journal of Acupuncture Research
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• v.18 no.1
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• pp.186-201
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• 2001

Purpose ; In order to study the effect of Houttuyniae herbal acupuncture on growth of melanoma B 16 and immunity in mice, the Control group with normal saline acupunchure after subcutaneous inoculation of B16BL6 cells, the Sample I with Houttuyniae herbal acupuncture manufactured by distilled water extraction method after subcutaneous inoculation of B16BL6 cells and the Sample II with Houttuyniae herbal acupuncture manufactured by alcohol extraction method after subcutaneous inoculation of B16BL6 cells were divided. Methods ; To evauluate the effect of Houttuynias herbal acupuncture on growth of melanoma B16 in mice, the weight of mouse melanoma, body weight, spleen index( $\sqrt(Weight of spleen/Body weight){\times}100$), lymphocytes numbers in mouse peripheral blood and spleen tissue, the percentage of CD4+ T cells, CD8+ and the ratio of CD4+/CD8+ in mouse peripheral blood and spleen tissue are measured. Results ; In study of the weight of mouse melanoma, Houttuyniae herbal acupuncture groups(Sample I, Sample II) showed statistically significant inhibitory effect. And in study of effect of reduction of change in body weight of mouse, Sample I showed statistically significant inhibitory effect, too. In study of reduction of spleen index, lymphocytes numbers in mouse spleen tissue, and the percentage of CD4+ and the ratio of CD4+/CD8+ T cells in mouse peripheral blood, Houttuyniae herbal acupuncture groups(Sample I, Sample II ) showed with statistically significant inhibitory effect. Also, Sample I showed inhibitory effect of reduction of the percentage of CD8+ T cells in mouse peripheral blood and spleen tissue and Sample II showed inhibitory effect of reduction of the percentage of CD4+ and the ratio of CD4+/CD8+ T cells in mouse spleen tissue with statistical significance. Conclusions ; The inhibitory effect of reduction of lymphocytes numbers in mouse peripheral blood and IL-2 productivity, Houttuyniae herbal acupuncture groups(Sample I, Sample II) didn't show significant effect.

• Applied Biological Chemistry
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• v.48 no.3
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• pp.273-279
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• 2005

To develop cosmetics using Jindalae flowers (Rhododendron mucronulatum), the surface tensions of extracts were measured and the properties and stability of cream with extracts were investigated. The surface tension of 0.1% ethanol extract was 30.42 mN/m and that of distilled water was 72.2 mN/m. The surface tension of cream with 0.1% ethanol extract was similar to that of sample cream and the measured pH were weakly alkalic. The surface tension of 1% ethanol extract was the lowest value of 24.98 mN/m, the measured pH of cream with 1% ethanol extract was weakly acidic and the particle size of cream was stable. According to an oscillatory test, linear viscoelastic region was extended by adding of 1% water extract and 1% ethanol extract to cream, indicating that the cream had greater enhanced resistance for preserving inner structure as compared to outside stress.　Besides, as a result of the diminished loss angle of ethanol extract cream, the elasticity of cream was increased more than that of sample cream and cream with 0.1% ethanol extract. In contrast, in the case of the increased loss angle of water extract cream, the viscosity of cream was increased. In conclusion, Rhododendron mucronulatum can be deliberated as a cosmetic material because 0.1% water and ethanol extracts showed efficacious physiological activities and cream with 1% extracts could extend linear viscoelastic region.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.245-253
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• 2010

The base sequences representing human- and cow-specific 168 rRNA gene markers identified in a T-RFLP analysis were recovered from clone libraries. The human- and cow-specific primers were designed from these sequences and their specificities were analyzed with fecal DNAs from human, cow, and pig. The AllBac primer set showed positive results for all human, cow, and pig samples, whereas the human-specific primer set showed positive result only for the human sample but not for the cow or pig samples. Likewise, the cow-specific primer set showed positive results only for the cow sample but not for the human or pig samples. Real-time PCR assay with these primers was developed for the identification and quantification of fecal pollution in the river water. The human- and cow-specific markers were detected in the order of 9 $\log_{10}$ copies per gram wet feces, which were two orders of magnitude lower than those of total Bacteroidales. For the river water samples, the human-specific marker was detected in $1.7-6.2\;\log_{10}$ copies/100 ml water, which was 2.4-4.9 orders of magnitude lower than those of total Bacteroidales. There was no significant correlation between total Bacteroidales and conventional fecal indicators, but there was a high correlation between Bacteroidales and the human-specific marker. This assay could reliably identify and quantify the fecal pollution sources, enabling effective measures in the watersheds and facilitating water quality management.

• Journal of the Korean Chemical Society
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• v.21 no.5
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• pp.372-378
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• 1977

The following new techniques have been developed: (A); To a 500ml of sample water, it was adjusted pH 10 with ammonia-anmonium citrate, added 10ml of 1${\%}$ sodium diethyldithiocarbamate and extracted three times with 10ml of CHCl3. The extract was shaken with 10ml of 0.05N $HCl-4{\times}10^{-4}M\;HgCl_2$. The aqueous solution was added 2ml of 2N KCl and washed two times with 10ml of pure $CHCl_3$, and then recorded square wave polarograms. (B); To a 500ml of sample water adjusted pH 10 with ammonia-ammonium citrate, it was added 2ml of 1${\%}$ 8-hydroxyquinoline and extracted three times with 10ml $CHCl_3$. The separated $CHCl_3$ phase was shaken with 10ml of 0.2 N HCl. The aqueous solution was recorded polarograms directly. These methods can be used for determination of the ppb order of metal in water with an error of ${\pm}10{\%}$. The method (B) can not be used for the determination of zinc on account of the free 8-hydroxyquinoline.

• Journal of Environmental Policy
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• v.7 no.3
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• pp.121-140
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• 2008

The impervious cover ratio has been used as an important measure for tracing water environment characteristics in watershed. Impervious cover is divided into total impervious cover and effective impervious cover, and its size varies depending on the land use characteristics of a watershed. Total impervious cover can be easily measured using existing land use maps or land cover map, while it takes a considerable amount of time and labor to measure the effective impervious cover, as water flow should be identified at each site. This study is intended to calculate the total impervious cover and effective cover of a sample site, compare their characteristics, and find a method to apply effective and total impervious cover ratios toward watershed management. The analysis of the sample site showed that the effective impervious cover rate(39.7%) was less than the total impervious cover rate(43%). This suggests that it would be acceptable, in terms of time and cost, if total impervious cover is applied as the representative impervious cover ratio of a watershed considering that it was used as basic data to analyze the effect that impervious cover has on the water environment.

• Journal of environmental and Sanitary engineering
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• v.23 no.4
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• pp.37-44
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• 2008

In this study we developed the analytical methods for the determination of three neurotoxin; anatoxin-a, saxitoxin and neosaxitoxin using HPLC/FLD system and this analytical methods were applied to real sample; algae culture and algae extracts. For the HPLC/FLD analysis of anatoxin-a samples were concentrated on WCX(Weak Cation Exchanger) SPE and then anatoxin-a in concentrate was derivatized with NBD-F solution. Supernatant was injected on HPLC system. For the HPLC/FLD analysis of saxitoxin and neosaxitoxin samples were separated on the column and then derivatizied by post column reactor for fluorescen detection. For post column reaction of saxitoxin we feed two kinds of reaction solution; Oxidizing Reagent of which composition was periodic acid(7mM) in 50mM potassium phosphate buffer, pH 9 and acidifying reagent of which Composition was 0.5M acetic acid. The LOD value for anatoxin-a, saxitoxin and neosaxitoxin in HPLC/FLD method was 24.3 ng. $35{\mu}g/L$, $27{\mu}g/L$ respectively. We determined the anatoxin-a content of lyophilized anabaena flos-aquae and $20{\mu}g/g$ d.w. of anatoxin-a was detected. We analyzed saxitoxin and neosaxitoxin in algae culture media and extracts of lypopyllized algal cell cultured and that of Deachung reservior. Saxitoxin and neosaxitoxin in real sample were below the limit of detection. Although there are various water treatment processes for removing neurotoxins were suggested no process give simultaneous and complete removal of neurotoxins. It was cocluded that nanofiltration which reject material by size can be a process for removal of neurotoxins.

• Korean Journal of Ecology and Environment
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• v.42 no.3
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• pp.303-316
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• 2009

Benthic macroinvertebrate communities collected from eight different streams in South Korea were analyzed to compare community and biological indices across different levels of water pollution. The Self-Organizing Map (SOM) was utilized to provide overview on association of the proposed indices. The sample sites were accordingly clustered according to the gradient of pollution on the SOM. While the general trends of the indices were commonly observable according to different levels of pollution, the detailed differences among the indices were also illustrated on the SOM. The conventional diversity and evenness indices tended to be high even though the water quality state was poor representing relatively weak gradient at polluted sites, while the index presenting the saprobic degree such as family biotic index showed the stronger gradient at the polluted area and was robust to present the gradient. Our results also confirmed the general characterization of two indices: The Shannon index is more strengthened by the number of species occurring at the sample sites, while the Simpson index is more influenced by the degree of evenness among the species. The patterning based on the SOM was efficient in comparatively characterizing the proposed indices to present ecological states and water quality.

• The Journal of Internal Korean Medicine
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• v.10 no.1
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• pp.81-91
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• 1989

It has been known for a long time that Palmiwon water extract can be prescribed for the treatment of deficiency of Yang in oriental medicine. But the exact mechanism of it has not been Known yet. In the present experiments, it was attempted to explain the effect of Palmiwon water extract on the serum Hormone level in rabbits and the antifatigue in mice. Palmiwon water extract oral intake showed a increase of serum hormone level in rabbits and the effects of the antifatigue in mice. The results obtained were as follows. 1. The administration of this medicine was observed to cause continuously a significant increase of the testosterone content in the serum from the 7th day to the 21th day. 2. The estradiol centent in the serum, as compared with the control experiment, was seen to increase significantly by the administration of this medicine from the 7th day to the 21th day. 3. The LH and FSH content in the serum, as compared with the control experiment, indicated a tendency toward an increase by the administration of this medicine from the 7th day to the 21 th day, but with no significant difference. 4. In the effect to Palniwon on the antifatigue in mice, swimming time was prolonged in both sample A, B groups than control group, and significance revealed in both sample A, B groups. These data suggest that Palmiwon water extract can increase serum hormone level and that it can have therapeutic effectiveness to the antifatigue.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.3
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• pp.20-27
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• 2008

This study was conducted to investigate the influence of thinning and pruning on characteristics of forest stand, mesopore ratio and soil water content at the Pinus koraiensis stands in Gwangneung, Gyeonggido. The Pinus koraiensis had been planted in 1976 and thinning and pruning were carried out in 1996. A sample area survey was conducted at experimental plots (thinned and unthinned) in 1998 and 2005, and mesopore ratio and soil water content have been monitored from 2000 to present. Average tree height of the thinned plot increased from 10.9m to 13.2m and from 10.3m to 12.8m for the unthinned plot. Average D.B.H of the thinned plot increased from 15.9cm to 21.1cm and from 14.5cm to 16.7cm for the unthinned plot during the period 1998-2005. Crown density at the thinned plot increased from 81.5% to 95.0% and from 89.5% to 95.0% for the unthinned plot during the period 1998-2005. Mesopore ratio (pF2.7) of A layer soil at the thinned plot was 40.1% while that of the unthinned plot was 37.3%. Changes of mesopore ratio at unthinned plot were not associated with stand age, but those at thinned plot had increased and then decreased, showing declining of the practice effect. Average soil water content at the thinned plot were 23.7% and 22.4% for the unthinned plot. Soil watercontents at both plots have been increased with increase in stand age. But the difference of soil watercontent at each plot has been decreased, especially at the depth of 10cm.

• Bulletin of the Korean Chemical Society
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• v.27 no.4
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• pp.563-567
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• 2006

The application of a synergistic solvent extraction by the formation of ternary complex with pyrocatechol violet (PV) and benzalkonium chloride (BC) was studied for determination of trace Zn(II) in water samples. The pH of sample solution and the amount of PV and BC added were optimized for the formation of the stable complex, a proper solvent was selected for the effective extraction, and the concentration of nitric acid was fixed for the back extraction of the complex from the solvent. After the ionic strength of 100 mL sample solution was adjusted to 0.1 M by adding NaCl and the pH was fixed at 9 with a carbonate buffer, 1.0 mL of 2% PV solution was added to form Zn(II)-PV complex then the Zn(II)-PV/BC ternary complex was made by adding 1.0 mL of 10% BC solution. The ternary complex was extracted into 10 mL of MIBK. And the ternary complex was back-extracted with 10 mL of 1.0 mol/L nitric acid to determine Zn(II) by a flame atomic absorption spectrophotometer (flame-AAS). The interference of concomitant ions on the extraction of Zn(II) was investigated. This procedure was applied to the analysis of three real samples such as Dalbang-dam water, laboratory tap water and Jungnajin seawater. The recoveries of Zn(II) in spiked samples were 86.58-104.1%.